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Expression and Purification of Recombinant Hepatitis Delta Virus(HDV) Antigen for Use in a Diagnostic ELISA for HDV Infection Using the High-Density Fermentation Strategy in Escherichia coli

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Biomedical and Environmental Sciences 2016年第6期29卷 417-423页

作者： lu xue xin YI Yao SU Qiu Dong BI Sheng Li National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

Objective Hepatitis Delt a Virus（HDV） antigen is widely used as a capture antigen in ELISAs for the identification of HDV infection; large amounts of recombinant HDV antigen with active antigenicity are required for this purpose. Methods Reconstruct the gene of HDV antigen based on the bias code of Escherichia coli, the recombinant protein expresses by high-density fermentation with fed-batch feeding strategy, and purify by immobilized metal chromatography. The sensitivity and specificity of this antigen detect by ELISA method. Results The expression of HDV antigen can reach 20% of the total cell mass in the soluble form. The recombinant HDV antigen can be conveniently purified（98%） by immobilized metal ion affinity chromatography（IMAC） using the interaction between a His-tag and nickel ions. Production of recombinant HDV antigen can reach 0.5 g/L under conditions of high-density cell fermentation. Applied to the diagnostic ELISA method, the recombinant HDV antigen shows excellent sensitivity（97% for IgM and 100% for IgG） and specificity（100% for IgG and IgM） for the detection of anti-HDV antibodies. Conclusion Expression and purification the recombinant HDV antigen as a candidate protein for application in a diagnostic ELISA for HDV infection. Large-scale production of the protein can be achieved using the high-density fermentation strategy.

关键词： ELISA diagnosis, HDV High-density cell fermentation IMAC

ERβ-activated LINC01018 promotes endometriosis development by regulating the CDC25C/CDK1/CyclinB1 pathway

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Journal of Genetics and Genomics 2024年第6期51卷 617-629页

作者： Ruihui lu Jingwen Zhu xin Li Cheng Zeng Yan Huang Chao Peng Yingfang Zhou Qing xue Department of Obstetrics and Gynecology Peking University First HospitalBeijing 100034China

Endometriosis refers to as an estrogen-dependent *** receptorβ(ERB),the main estrogen receptor subtype which is encoded by the estrogen receptor 2(ESR2)gene,can mediate the action of estrogen in *** selective estrogen receptor modulators can target the ERβ,they are not specific due to the wide distribution of ERβ.Recently,long noncoding RNAs have been implicated in ***,we aim to explore and validate the downstream regulatory mechanism of ERβ,and to investigate the potential role of long intergenic noncoding RNA 1018(LINC01018)as a nonhormonal treatment for *** study demonstrates that the expression levels of ESR2 and LINCo1018 are increased in ectopic endometrial tissues and reveals a significant positive correlation between the ESR2 and LINCo1018 ***,ERβdirectly binds to an estrogen response element located in the LINCO1018 promoter region and activates LINC01018 ***,ERβcan regulate the CDC25C/CDK1/CyclinB1 pathway and promote ectopic endometrial stromal cell proliferation via LINC01018 in *** with these findings,the knockdown of LINC01018 inhibits endometriotic lesion proliferation in *** summary,our study demonstrates that the ERβ/LINC01018/CDC25C/CDK1/CyclinB1 signaling axis regulates endometriosis progression.

关键词： Estrogen receptorβ Long intergenic noncoding RNA 1018 (LINC01018) Endometriosis Cell cycle Proliferation

Impact response and energy absorption of metallic buffer with entangled wire mesh damper

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Defence Technology（防务技术） 2024年第5期35卷 137-150页

作者： Chao Zheng Jun Wu Mangong Zhang xin xue Institute of Metal Rubber&Vibration Noise School of Mechanical Engineering and AutomationFuzhou UniversityFuzhou 350116China Wuhan Second Ship Design and Research Institute Wuhan 430205China

An innovative metallic buffer consisting of series-connected hat-shaped entangled wire mesh damper(EWMD)and parallel springs are proposed in this work to enhance the reliability of engineering *** impact response and the energy dissipation mechanism of hat-shaped EWMD under different quasi-static compression deformations(2-7 mm)and impact heights(100-200 mm)are investigated using experimental and numerical *** results demonstrate distinct stages in the quasi-static mechanical characteristics of hat-shaped EWMD,including stiffness softening,negative stiffness,and stiffness *** loss factor gradually increases with increasing compression deformation before entering the stiffness hardening *** impact loads,the hat-shaped EWMD exhibits optimal impact energy absorption when it enters the negative stiffness stage(150 mm),resulting in the best impact isolation effect of metallic ***,the impact energy absorption significantly decreases when hat-shaped EWMD enters the stiffness hardening ***,quasi-static compression analysis after experiencing different impact loads reveals the disappearance of the negative stiffness ***,with increasing impact loads,the stiffness hardening point progressively shifts to an earlier stage.

关键词： Metallic buffer Hat-shaped EWMD Drop impact Energy absorption characteristics Mechanical behavior

Investigations of methane adsorption characteristics on marine-continental transitional shales and gas storage capacity models considering pore evolution

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Petroleum Science 2024年第4期21卷 2273-2286页

作者： Chen-Gang lu Xian-Ming Xiao Zhen-Qian xue Zhang-xin Chen Yin-Tao Dong Yue Feng Gang Li School of Energy Resources China University of Geosciences (Beijing) School of Civil and Environmental Engineering Nanyang Technological University Chemical and Petroleum Engineering University of Calgary Eastern Institute of Technology (tentative) CNOOC Research Institute

Methane adsorption is a critical assessment of the gas storage capacity(GSC) of shales with geological conditions. Although the related research of marine shales has been well-illustrated, the methane adsorption of marine-continental transitional(MCT) shales is still ambiguous. In this study, a method of combining experimental data with analytical models was used to investigate the methane adsorption characteristics and GSC of MCT shales collected from the Qinshui Basin, China. The Ono-Kondo model was used to fit the adsorption data to obtain the adsorption parameters. Subsequently, the geological model of GSC based on pore evolution was constructed using a representative shale sample with a total organic carbon(TOC) content of 1.71%, and the effects of reservoir pressure coefficient and water saturation on GSC were explored. In experimental results, compared to the composition of the MCT shale, the pore structure dominates the methane adsorption, and meanwhile, the maturity mainly governs the pore structure. Besides, maturity in the middle-eastern region of the Qinshui Basin shows a strong positive correlation with burial depth. The two parameters, micropore pore volume and non-micropore surface area, induce a good fit for the adsorption capacity data of the shale. In simulation results, the depth,pressure coefficient, and water saturation of the shale all affect the GSC. It demonstrates a promising shale gas potential of the MCT shale in a deeper block, especially with low water saturation. Specifically,the economic feasibility of shale gas could be a major consideration for the shale with a depth of 60% in the Yushe-Wuxiang area. This study provides a valuable reference for the reservoir evaluation and favorable block search of MCT shale gas.

关键词： High-pressure methane adsorption Marine-continental transitional shale gas Ono-Kondo model Adsorption thermodynamics Gas storage capacity model

Structural Engineering of Hierarchical Magnetic/Carbon Nanocomposites via In Situ Growth for High-Efficient Electromagnetic Wave Absorption

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Nano-Micro Letters 2024年第9期16卷 262-278页

作者： Xianyuan Liu Jinman Zhou Ying xue Xianyong lu Key Laboratory of Bio‑Inspired Smart Interfacial Science and Technology of Ministry of Education School of ChemistryBeihang UniversityBeijing 100191People’s Republic of China

Materials exhibiting high-performance electromagnetic wave absorption have garnered considerable scientific and technological attention,yet encounter significant *** new materials and innovative structural design concepts is crucial for expanding the application field of electromagnetic wave ***,hierarchical structure engineering has emerged as a promising approach to enhance the physical and chemical properties of materials,providing immense potential for creating versatile electromagnetic wave absorption ***,an exceptional multi-dimensional hierarchical structure was meticulously devised,unleashing the full microwave attenuation capabilities through in situ growth,selfreduction,and multi-heterogeneous interface *** hierarchical structure features a three-dimensional carbon framework,where magnetic nanoparticles grow in situ on the carbon skeleton,creating a necklace-like ***,magnetic nanosheets assemble within this *** impedance matching was achieved by precisely adjusting component proportions,and intelligent integration of diverse interfaces bolstered dielectric *** obtain Fe_(3)O_(4)-Fe nanoparticles/carbon nanofibers/Al-Fe_(3)O_(4)-Fe nanosheets composites demonstrated outstanding performance with a minimum reflection loss(RLmin)value of−59.3 dB and an effective absorption bandwidth(RL≤−10 dB)extending up to 5.6 GHz at 2.2 *** notable accomplishments offer fresh insights into the precision design of high-efficient electromagnetic wave absorption materials.

关键词： Electromagnetic wave absorption Hierarchical structure In situ growth Self-reduction

Random Forest-Based Fatigue Reliability-Based Design Optimization for Aeroengine Structures

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Computer Modeling in Engineering & Sciences 2024年第7期140卷 665-684页

作者： xue-Qin Li lu-Kai Song School of Energy and Power Engineering Beihang UniversityBeijing100191China Department of Mechanical Engineering The Hong Kong Polytechnic UniversityKowloonHong Kong999077China Research Institute of Aero-Engine Beihang UniversityBeijing100191China

Fatigue reliability-based design optimization of aeroengine structures involves multiple repeated calculations of reliability degree and large-scale calls of implicit high-nonlinearity limit state function,leading to the traditional direct Monte Claro and surrogate methods prone to unacceptable computing efficiency and *** this case,by fusing the random subspace strategy and weight allocation technology into bagging ensemble theory,a random forest(RF)model is presented to enhance the computing efficiency of reliability degree;moreover,by embedding the RF model into multilevel optimization model,an efficient RF-assisted fatigue reliability-based design optimization framework is *** the low-cycle fatigue reliability-based design optimization of aeroengine turbine disc as a case,the effectiveness of the presented framework is *** reliabilitybased design optimization results exhibit that the proposed framework holds high computing accuracy and computing *** current efforts shed a light on the theory/method development of reliability-based design optimization of complex engineering structures.

关键词： Random forest reliability-based design optimization ensemble learning machine learning

Building Feedback-Regulation System Through Atomic Design for Highly Active SO_(2)Sensing

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Nano-Micro Letters 2024年第7期16卷 343-357页

作者： xin Jia Panzhe Qiao Xiaowu Wang Muyu Yan Yang Chen Bao-Li An Pengfei Hu Bo lu Jing Xu Zhenggang xue Jiaqiang Xu NEST Lab Department of ChemistryCollege of SciencesShanghai UniversityShanghai 200444People’s Republic of China Shanghai Synchrotron Radiation Facility Zhangjiang LabShanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204People’s Republic of China School of Chemistry and Materials Science University of Science and Technology of ChinaHefei 230026People’s Republic of China Key Laboratory of Organic Compound Pollution Control Engineering(MOE) School of Environmental and Chemical EngineeringShanghai UniversityShanghai 200444People’s Republic of China Shanghai University Instrumental Analysis&Research Center of Shanghai UniversityShanghai 200444People’s Republic of China

Reasonably constructing an atomic interface is pronouncedly essential for surface-related gas-sensing ***,we present an ingen-ious feedback-regulation system by changing the interactional mode between single Pt atoms and adjacent S species for high-efficiency SO_(2)*** found that the single Pt sites on the MoS_(2)surface can induce easier volatiliza-tion of adjacent S species to activate the whole inert S ***,the activated S species can provide a feedback role in tailoring the antibonding-orbital electronic occupancy state of Pt atoms,thus creating a combined system involving S vacancy-assisted single Pt sites(Pt-Vs)to synergistically improve the adsorption ability of SO_(2)gas ***-more,in situ Raman,ex situ X-ray photoelectron spectroscopy testing and density functional theory analysis demonstrate the intact feedback-regulation system can expand the electron transfer path from single Pt sites to whole Pt-MoS_(2)supports in SO_(2)gas *** with wireless-sensing modules,the final Pt1-MoS_(2)-def sensors array can further realize real-time monitoring of SO_(2)levels and cloud-data storage for plant *** a fundamental understanding of the intrinsic link between atomic interface and sensing mechanism is thus expected to broaden the rational design of highly effective gas sensors.

关键词： Feedback-regulation system Atomic interface SO_(2)sensor Single-atom sensing mechanism Intelligent-sensing array

Advance on Chinese Medicine for Hypertensive Renal Damage:Focus on the Complex Molecular Mechanisms

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Chinese Journal of Integrative Medicine 2024年第10期30卷 938-948页

作者： lu Yan XIE xue-na xin Qi-qi YUAN Rong MIAO Yu CONG Wei-hong CHEN Ke-ji Laboratory of Cardiovascular Diseases Xiyuan Hospital of China Academy of Chinese Medical SciencesBeijing100091China School of Pharmacy Macao University of Science and TechnologyTaipaMacao999078China National Clinical Research Center for Chinese Medicine Cardiology Beijing100091China

Hypertensive renal damage(HRD)is a major cause of end-stage renal *** the causes of end-stage renal disease,HRD accounts for nearly 34%of the total number of *** treatment is primarily drug-based,but therapeutic efficacy is less effective and can have serious side *** medicine(CM)has significant advantages in the treatment of *** is rich in various active ingredients and has the property of targeting multiple targets and ***,the regulatory network of CM on disease is complex.A large number of CM have been employed to treat HRD,either as single applications or as part of compound *** key possible mechanisms of CM for HRD include regulation of the renin-angiotensin-aldosterone system,antioxidation,anti-inflammation,rescue of endothelial function,regulation of vasoactive substance secretion and obesity-related factors,*** review summarized and discussed the recent advance in the basic research mechanisms of CM interventions for HRD and pointed out the challenges and future prospects.

关键词： Chinese medicine hypertensive renal damage molecular mechanisms research progress

Microstructure evolution and strengthening mechanism of high -performance powder metallurgy TA15 titanium alloy by hot rolling

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International Journal of Minerals,Metallurgy and Materials 2024年第6期31卷 1426-1436页

作者： Ying Gao Ce Zhang Jiazhen Zhang xin lu Beijing Advanced Innovation Center for Materials Genome Engineering State Key Laboratory for Advanced Metals and MaterialsInstitute for Advanced Ma-terials and TechnologyUniversity of Science and Technology BeijingBeijing 100083China National Engineering Research Center for Advanced Rolling Technology National Engineering Research Center for Flat Rolling EquipmentInstitute of Engin-eering TechnologyUniversity of Science and Technology BeijingBeijing 100083China

Hot deformation of sintered billets by powder metallurgy(PM)is an effective preparation technique for titanium alloys,which is more significant for high-alloying *** this study,Ti–6.5Al–2Zr–Mo–V(TA15)titanium alloy plates were prepared by cold press-ing sintering combined with high-temperature hot *** microstructure and mechanical properties under different process paramet-ers were *** microscope,electron backscatter diffraction,and others were applied to characterize the microstructure evolution and mechanical properties strengthening *** results showed that the chemical compositions were uniformly dif-fused without segregation during sintering,and the closing of the matrix craters was accelerated by increasing the sintering *** block was hot rolled at 1200℃ with an 80%reduction under only two passes without *** strength and elongation of the plate at 20–25℃ after solution and aging were 1247 MPa and 14.0%,respectively,which were increased by 24.5%and 40.0%,respect-ively,compared with the as-sintered alloy at 1300℃.The microstructure was significantly refined by continuous dynamic recrystalliza-tion,which was completed by the rotation and dislocation absorption of the substructure surrounded by low-angle grain *** hot rolling combined with heat treatment,the strength and plasticity of PM-TA15 were significantly improved,which resulted from the dense,uniform,and fine recrystallization structure and the synergistic effect of multiple slip systems.

关键词： elemental powder powder metallurgy titanium alloy hot rolling strength and plasticity