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Journal of Integrative Agriculture 2024年第5期23卷 1685-1702页

作者： Qianqian Chen Qian Zhao Baoxing Xie Xing lu Qi Guo Guoxuan Liu Ming Zhou Jihui Tian Weiguo lu kang Chen Jiang Tian Cuiyue Liang Root Biology Center State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of Natural Resources and EnvironmentSouth China Agricultural UniversityGuangzhou 510642China College of Natural Resources and Environment South China Agricultural UniversityGuangzhou 510642China Henan Academy of Crops Molecular Breeding Henan Academy of Agricultural SciencesZhengzhou 450002China

Bacteria play critical roles in regulating soil phosphorus(P) cycling. The effects of interactions between crops and soil P-availability on bacterial communities and the feedback regulation of soil P cycling by the bacterial community modifications are poorly understood. Here, six soybean(Glycine max) genotypes with differences in P efficiency were cultivated in acidic soils with long-term sufficient or deficient P-fertilizer treatments. The acid phosphatase(AcP) activities, organic-P concentrations and associated bacterial community compositions were determined in bulk and rhizosphere soils. The results showed that both soybean plant P content and the soil AcP activity were negatively correlated with soil organic-P concentration in P-deficient acidic soils. Soil P-availability affected the ɑ-diversity of bacteria in both bulk and rhizosphere soils. However, soybean had a stronger effect on the bacterial community composition, as reflected by the similar biomarker bacteria in the rhizosphere soils in both P-treatments. The relative abundance of biomarker bacteria Proteobacteria was strongly correlated with soil organic-P concentration and AcP activity in low-P treatments. Further high-throughput sequencing of the phoC gene revealed an obvious shift in Proteobacteria groups between bulk soils and rhizosphere soils, which was emphasized by the higher relative abundances of Cupriavidus and Klebsiella, and lower relative abundance of Xanthomonas in rhizosphere soils. Among them, Cupriavidus was the dominant phoC bacterial genus, and it was negatively correlated with the soil organic-P concentration. These findings suggest that soybean growth relies on organic-P mineralization in P-deficient acidic soils, which might be partially achieved by recruiting specific phoCharboring bacteria, such as Cupriavidus.

关键词： organic phosphorus acid phosphatase soybean bacterial community phoC-harboring bacteria rhizosphere

Automated federated learning‐based adversarial attack and defence in industrial control systems

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IET Cyber-Systems and Robotics 2024年第2期6卷 109-127页

作者： Guo-Qiang Zeng Jun-Min Shao kang-Di lu Guang-Gang Geng Jian Weng College of Cyber Security Jinan UniversityGuangzhouChina National Joint Engineering Research Center of Network Security Detection and Protection Technology Jinan UniversityGuangzhouChina College of Information Science and Technology Donghua UniversityShanghaiChina

With the development of deep learning and federated learning(FL),federated intrusion detection systems(IDSs)based on deep learning have played a significant role in securing industrial control systems(ICSs).However,adversarial attacks on ICSs may compromise the ability of deep learning-based IDSs to accurately detect cyberattacks,leading to serious ***,in the process of generating adversarial samples,the selection of replacement models lacks an effective method,which may not fully expose the vulnerabilities of the *** authors first propose an automated FL-based method to generate adversarial samples in ICSs,called AFL-GAS,which uses the prin-ciple of transfer attack and fully considers the importance of replacement models during the process of adversarial sample *** the proposed AFL-GAS method,a lightweight neural architecture search method is developed to find the optimised replacement model composed of a combination of four lightweight basic ***,to enhance the adversarial robustness,the authors propose a multi-objective neural archi-tecture search-based IDS method against adversarial attacks in ICSs,called MoNAS-IDSAA,by considering both classification performance on regular samples and adver-sarial robustness *** experimental results on three widely used intrusion detection datasets in ICSs,such as secure water treatment(SWaT),Water Distribution,and Power System Attack,demonstrate that the proposed AFL-GAS method has obvious advantages in evasion rate and lightweight compared with other four ***,the proposed MoNAS-IDSAA method not only has a better classification performance,but also has obvious advantages in model adversarial robustness compared with one manually designed federated adversarial learning-based IDS method.

关键词： adversarial attacks adversarial robustness automated federated learning Industrial control system intrusion

Coordinatively unsaturated cobalt single-atom nanozymes for visual pesticides detection by smartphone-based platform

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Nano Research 2024年第4期17卷 2298-2307页

作者： Fangning Liu Zhe Li Hengya Wei Peng Xu Ge kang Shicheng Zhu Tingting Wang Ruxue He Chuanxia Chen Yizhong lu School of Materials Science and Engineering University of JinanJinan 250022China

By adjusting the coordination environment of single-atom catalysts,the enzyme-like activity can be finely tuned for highly sensitive ***,we demonstrated that coordinatively unsaturated cobalt-nitrogen sites doped within porous carbon(SA-CoN_(3))could serve as highly efficient oxidase *** with the typical planar four-coordination structure(SA-CoN_(4)),the as-obtained single-atom Co nanozymes anchored by three nitrogen atoms are found to display much higher oxidase-like catalytic *** theoretical and experimental analysis revealed that the coordinatively unsaturated Co sites could facilitate adsorption and activation of O_(2) molecule and thus improve their oxidase-like *** on the enhanced oxidase-like activity of SA-CoN_(3),a paper/smartphone sensor for organophosphorus pesticides(OPs)was successfully constructed and used to quantify glyphosate in environmental and food samples with a low detection limit of 0.66μ*** work not only highlights the important role of coordination unsaturation of SA nanozymes for promoting oxidase-like activity,but also provides an easy and cost-effective way to conduct effective quantification of OPs in the field.

关键词： single-atom nanozymes asymmetric organophosphorus pesticides oxygen adsorption smartphone

Design,preparation,application of advanced array structured materials and their action mechanism analyses for high performance lithium-sulfur batteries

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Journal of Energy Chemistry 2024年第2期89卷 266-303,I0007页

作者： Nanping Deng Xiaofan Feng Yongbing Jin Zhaozhao Peng Yang Feng Ying Tian Yong Liu lu Gao Weimin kang Bowen Cheng State Key Laboratory of Separation Membranes and Membrane Processes/National Center for International Joint Research on Separation Membranes School of Textile Science and EngineeringTiangong UniversityTianjin 300387China Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) Nankai UniversityTianjin 300071China

Lithium-sulfur battery(LSB)has brought much attention and concern because of high theoretical specific capacity and energy density as one of main competitors for next-generation energy storage *** widely commercial application and development of LSB is mainly hindered by serious“shuttle effect”of lithium polysulfides(Li PSs),slow reaction kinetics,notorious lithium dendrites,*** various structures of LSB materials,array structured materials,possessing the composition of ordered micro units with the same or similar characteristics of each unit,present excellent application potential for various secondary cells due to some merits such as immobilization of active substances,high specific surface area,appropriate pore sizes,easy modification of functional material surface,accommodated huge volume change,enough facilitated transportation for electrons/lithium ions,and special functional groups strongly adsorbing Li *** many novel array structured materials are applied to battery for tackling thorny problems mentioned *** this review,recent progresses and developments on array structured materials applied in LSBs including preparation ways,collaborative structural designs based on array structures,and action mechanism analyses in improving electrochemical performance and safety are ***,we also have detailed discussion for array structured materials in LSBs and constructed the structure-function relationships between array structured materials and battery ***,some directions and prospects about preparation ways,functional modifications,and practical applications of array structured materials in LSBs are *** hope the review can attract more researchers'attention and bring more studying on array structured materials for other secondary batteries including LSB.

关键词： Array structured materials Preparation methods and structural designs Action mechanism analyses Advanced Li-S batteries Excellent electrochemical performances and safety

Efficient and multiplex gene upregulation in plants through CRISPR-Cas-mediated knockin of enhancers

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Molecular Plant 2024年第9期17卷 1472-1483页

作者： Qi Yao Rundong Shen Yang Shao Yifu Tian Peijin Han Xuening Zhang Jian-kang Zhu Yuming lu Shanghai Collaborative Innovation Center of Agri-Seeds Joint Center for Single-Cell BiologySchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Shanghai Center for Plant Stress Biology Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China University of the Chinese Academy of Sciences BeijingChina College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China institute of Advanced Biotechnology and School of Medicine Southern University of Science and TechnologyShenzhen 518055China

Geneupregulation through genome editing is important for plant research and *** insertion of short transcriptional enhancers(STEs)into gene promoters may offer a universal solution akin to transgene-mediated overexpression while avoiding the drawbacks associated with ***,we introduce an“in locus activation”technique in rice that leverages well-characterized STEs for refined,heritable,and multiplexed gene *** address the scarcity of potent enhancers,we developed a large-scale mining approach and discovered a suite of STEs that are capable of enhancing gene expression in rice *** in locus integration of these STEs into eight rice genes resulted in substantial tran-scriptional upregulation in the edited plants,with up to 869.1-fold increases in their transcript ***-ploying a variety of STEs,we achieved delicate control of gene expression,enabling the fine-tuning of key phenotypic traits such as plant *** approach also enabled efficient multiplexed gene upregu-lation,with up to four genes activated simultaneously,significantly enhancing the nicotinamide mononucleotide metabolic ***,heritability studies from the To to T3 generations confirmed the stable and heritable nature of STE-driven gene ***,our work demon-strates that coupled with STE mining,leveraging genome editing for in locus activation and gene upregu-lation holds great promise to be widely adopted in fundamental plant research and crop breeding.

关键词： genome editing short transcriptional enhancers gene upregulation crop breeding

Hybrid Method: Review, Modularization and Application

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Systems Science and Systems Engineering 1999年第4期9卷 2-8页

作者： XU Yi\|ping,\ CHEN Xi\|kang,\ lu Yan Institute of Systems Science, Academia Sinica, Beijing 100080,China

The objective of this approach is to introduce the idea of modular design into the construction of hybrid model, thus, each step has it own unique function, the entries of superior data decrease and the directions of data flow are more clear. Although the approach itself cannot guarantee the accuracy of the target table which depends on the accuracy of the survey data, experience of experts and assumption in deriving, it does can help constructors to arrange their thoughts, thereby subjective errors could be brought down significantly. As an example, a case analysis is employed to show the effectiveness of the method in detail.

关键词： hybrid method coefficient updating method input\|output model

用航测技术测绘数字化工点地形图和横断面图的研究

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铁道学报 2006年第5期28卷 118-124页

作者：王智卢建康铁道第二勘察设计院测绘分院四川成都610031

利用航测技术测绘大比例尺工点图和横断面图,是铁路勘测设计部门重点研究的关键技术问题之一,其主要难点在于采用小比例尺航摄,精度达不到要求;采用大比例尺航摄,航摄费用过高。本文作者利用GPS全球定位技术使航测外业控测精度由分米级... 详细信息

利用航测技术测绘大比例尺工点图和横断面图,是铁路勘测设计部门重点研究的关键技术问题之一,其主要难点在于采用小比例尺航摄,精度达不到要求;采用大比例尺航摄,航摄费用过高。本文作者利用GPS全球定位技术使航测外业控测精度由分米级达到厘米级;航测内业采用数字摄影测量工作站,全面开展数字测图,精度得到了提高。在理论分析和精度估算后,选定合适的航摄比例尺,采用一些技术措施,结合郑西客运专线勘测设计试验证明,用航测方法测绘工点地形图和横断面图的精度满足铁路勘测设计的要求,并总结出一套作业方法和要求。数字化产品也为设计阶段进行计算机辅助设计创造了条件。

关键词：航测数字化工点地形图横断面

Electrostatic Encapsulation of Cobalt Ions into Crystalline Framework Derived Polymer Aerogel:Ultra-light,Pressure Resistant,Hydrophobic,Photothermal Conversion,Heat Insulation and Infrared Stealth

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Chinese Journal of Polymer Science 2024年第7期42卷 946-957,I0007页

作者： Yi-Fan kang Jin-lu Tang Ming Song Wei Wang Jia-Cheng Ma Zhan-You Ji Tong Liu Wen-Huan Huang China Key Laboratory of Chemical Additives for China National Light Industry College of Chemistry and Chemical EngineeringShaanxi University of Science and TechnologyXi’an710021China College of New Energy Xi’an Shiyou UniversityXi’an710065China Xi’an Key Laboratory of Functional Supramolecular Structure and Materials College of Chemistry and Materials ScienceNorthwest UniversityXi’an710127China

A variety of electromagnetic wave absorption materials(EMWAs)have been reported,but the integration of powder materials and multifunctional devices should be investigated in-depth to adapt to practical ***,carbon-coated cobalt composites were prepared by adsorbing magnetic metal cations into an anionic crystalline framework through an electrostatic encapsulate *** reflection loss(RL_(min))of-40.49 d B and effective absorption bandwidth(EAB)of 5.36 GHz(RLlulose(CMC)aerogel to create an ultra-lightweight composite aerogel that is compressive resistant and heat-holding while also having photothermal conversion *** hydrophobic modification makes it more widely *** study provides a new strategy for EWAMs to integrate versatility and improve their application prospects.

关键词： Electrostatic incorporation Magnetic nanoparticle Co@CN composites Electromagnetic wave absorbing Metal-organic framework

Exploring the precision redox map during fasting-refeeding and satiation in ***

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Stress Biology 2023年第1期3卷 187-200页

作者： Xinhua Qiao lu kang Chang Shi Aojun Ye Dongli Wu Yuyunfei Huang Minghao Deng Jiarui Wang Yuzheng Zhao Chang Chen National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China School of Basic Medical Sciences of Southwest Medical University Luzhou 646000China University of Chinese Academy of Sciences Beijing 100049China School of Pharmacy East China University of Science and TechnologyShanghai 200237China

Fasting is a popular dietary strategy because it grants numerous advantages,and redox regulation is one mecha-nism ***,the precise redox changes with respect to the redox species,organelles and tissues remain unclear,which hinders the understanding of the metabolic mechanism,and exploring the precision redox map under various dietary statuses is of great *** redox-sensitive *** strains stably expressing genetically encoded redox fluorescent probes(Hyperion sensing H_(2)O_(2) and Grx1-roGFP2 sensing GSH/GSSG)in three organelles(cytoplasm,mitochondria and endoplasmic reticulum(ER))were constructed in two tissues(body wall muscle and neurons)and were confirmed to respond to redox *** H_(2)O_(2) and GSSG/GSH redox changes in two tissues and three organelles were obtained by confocal microscopy during fasting,refeeding,and *** found that under fasting condition,H_(2)O_(2) decreased in most compartments,except for an increase in mitochondria,while GSSG/GSH increased in the cytoplasm of body muscle and the ER of *** refeeding,the redox changes in H_(2)O_(2) and GSSG/GSH caused by fasting were reversed in most organelles of the body wall muscle and *** the sati-ated state,H_(2)O_(2) increased markedly in the cytoplasm,mitochondria and ER of muscle and the ER of neurons,while GSSG/GSH exhibited no change in most organelles of the two tissues except for an increase in the ER of *** study systematically and precisely presents the redox characteristics under different dietary states in living animals and provides a basis for further investigating the redox mechanism in metabolism and optimizing dietary guidance.

关键词： Redox Fasting Refeeding Satiation Hyperion Grx1-roGFP2 Caenorhabditis elegans