检索结果-南通市图书馆

Neural Regeneration Research 2012年第22期7卷 1688-1694页

作者： jiang lu Kehuan lu Dongsheng Li State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangxi University Nanning 530004 Guangxi Zhuang Autonomous Region China Department of Anesthesiology Taihe Hospital Hubei University of Medicine Shiyan 442000 Hubei Province China Hubei Key Laboratory of Embryonic Stem Cell Research Taihe Hospital Hubei University of Medicine Shiyan 442000 Hubei Province China

In the present study, we investigated the dynamic expression of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway related factors in the process of in vitro hippocampal neural stem/progenitor cell differentiation from embryonic Sprague-Dawley rats or embryonic Kunming species mice, using fluorescent quantitative reverse transcription-PCR and western blot analyses. Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors. Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche. Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells.

关键词： neural stem cells neural progenitor cells fibroblast growth factor 8 Sonic Hedgehog signalpathway secretion dynamic differentiation neurons neural regeneration

Distribution and localization of fibroblast growth factor-8 in rat brain and nerve cells during neural stem/progenitor cell differentiation

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2012年第19期7卷 1455-1462页

作者： jiang lu Dongsheng Li Kehuan lu State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangxi University Nanning 530004 GuangxiZhuang Autonomous Region China Department of Anesthesiology Taihe Hospital Hubei University of Medicine Shiyan 442000 Hubei Province China Hubei Key Laboratory of Embryonic Stem Cell Research Taihe Hospital Hubei University of Medicine Shiyan 442000 Hubei ProvinceChina

The present study explored the distribution and localization of fibroblast growth factor-8 and its potential receptor, fibroblast growth factor receptor-3, in adult rat brain in vivo and in nerve cells during differentiation of neural stem/progenitor cells in vitro. Immunohistochemistry was used to examine the distribution of fibroblast growth factor-8 in adult rat brain in vivo. Localization of fibroblast growth factor-8 and fibroblast growth factor receptor-3 in cells during neural stem/progenitor cell differentiation in vitro was detected by immunofluorescence. Flow cytometry and immunofluorescence were used to evaluate the effect of an anti-fibroblast growth factor-8 antibody on neural stem/progenitor cell differentiation and expansion in vitro. Results from this study confirmed that fibroblast growth factor-8 was mainly distributed in adult midbrain, namely the substantia nigra, compact part, dorsal tier, substantia nigra and reticular part, but was not detected in the forebrain comprising the caudate putamen and striatum. Unusual results were obtained in retrosplenial locations of adult rat brain. We found that fibroblast growth factor-8 and fibroblast growth factor receptor-3 were distributed on the cell membrane and in the cytoplasm of nerve cells using immunohistochemistry and immunofluorescence analyses. We considered that the distribution of fibroblast growth factor-8 and fibroblast growth factor receptor-3 in neural cells corresponded to the characteristics of fibroblast growth factor-8, a secretory factor. Addition of an anti-fibroblast growth factor-8 antibody to cultures significantly affected the rate of expansion and differentiation of neural stem/progenitor cells. In contrast, addition of recombinant fibroblast growth factor-8 to differentiation medium promoted neural stem/progenitor cell differentiation and increased the final yields of dopaminergic neurons and total neurons. Our study may help delineate the important roles of fibroblast growth fa

关键词： fibroblast growth factor-8 fibroblast growth factor receptor-3 neural stem/progenitor celldifferentiation dopaminergic neurons midbrain neural regeneration

Study of parametric roll in oblique waves using a three-dimensional hybrid panel method

在线全文

学校读者我要写书评

暂无评论

Journal of Hydrodynamics 2020年第1期32卷 126-138页

作者： Min Gu Shu-xia Bu jiang lu jiangsu Key Laboralory of Green Ship Technology China Ship Scientific Research CenterWiuxi 214082.China

A reliable numerical tool for the prediction of the parametric roll is important in the development of the second generation intact stability *** main difficulty of the prediction of the parametric roll comes from the significant coupling of the roll with other motions,especially the heave and pitch *** this paper,the numerical method for determining the parametric roll is studied and the results are benchmarked against the model test ***,a 3 degrees-of-freedom(DOF)time domain hybrid panel method is validated by experimental data in regular oblique waves and irregular head ***,the verified 3D O F method is extended to a fully coupled 6 DOF method with the course ***,taking the C11 containership as the test case,the parametric roll in oblique waves is computed,and the effects of the surge,sway and yaw motions on the parametric roll are evaluated with the developed mathematical *** results show that the effects of the surge and sway motions on the parametric roll is insignificant,while the effects of the yaw motion are more *** results of the roll amplitudes and the standard deviation of the roll angles show that the model based on 3 DOF gives relatively conservative results,and can be directly used for the stability assessment of the parametric roll.

关键词： 6DOF mathematical model irregular oblique waves second generation intact stability in IMO

Some geometrical iteration methods for nonlinear equations

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Applied Mathematics(A Journal of Chinese Universities) 2008年第1期23卷 25-30页

作者： lu Xing-jiang QIAN Chun Dept. of Math Zhejiang Univ. Hangzhou 310027 China. College of Statist. and Comput. Sci. Zhejiang Gongshang Univ. Hangzhou 310018China.

This paper describes geometrical essentials of some iteration methods （e.g. Newton iteration, secant line method, etc.） for solving nonlinear equations and advances some geometrical methods of iteration that are fle... 详细信息

关键词： nonlinear equation iteration geometric method.

Molecular Cloning and Preliminary Analysis of a Fragile Site Associated Gene

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2006年第5期19卷 392-398页

作者： YI-WEN CAO CHUAN-lu jiang TAO jiang Department of Echography People＇s Armed Police General Hospital of Heilongjiang Province Harbin 150076 Heilongjiang China Department of Neurosurgery the Second Affiliated Hospital Harbin Medical University Harbin 150001 Heilongjiang China Department of Neurosurgery Neurosurgery Institute and Tiantan Hospital PUMC and CAMS Beijing 100050 China

Objective To analyze the molecular coining of a fragile site-associated gene. Methods Genomic Chinese hamster ovary （CHO） DNA library was constructed using high molecular weight CHO DNA partially digested with MboI restriction enzyme from cultured CHO cells. Screening of genomic DNA library followed the established procedures. Genomic CHO in the positive clones was sequenced. Appropriate primers were designed for the reverse transcriptase-polymerase chain reactions （RT-PCR）. The RT-PCR products were cloned into a pCRII TOPO vector and confirmed by DNA sequencing. Antibodies were prepared using synthetic peptides as antigens by immunizing the rabbits. Immunohistochemical analyses were performed to evaluate the expression of the novel gene in different tissues. Results To investigate the molecular mechanism underlying the initial events of mdrla amplification, we cloned lq31 fragile site DNA. Strikingly, we found that this fragile site contained a novel gene which was designated as a fragile site-associated （FSA） gene. FSA encoded an unusually large mRNA of - 16 kb. Full-length human FSA eDNA was cloned. FSA mRNA was expressed in many cultured cells and tissue types. Immunohistochemical analyses also revealed an expression pattern of the encoded proteins in postmitotic, well-differentiated epithelial compartments of many organs, including colon, mammary glands, ovary, prostate, and bladder. Conclusion FSA plays an important role in regulating mammalian epithelial cell growth and differentiation.

关键词： Fragile site DNA amplification Epithelial differentiation

A User-Friendly SSVEP-Based BCI Using Imperceptible Phase-Coded Flickers at 60Hz

在线全文

学校读者我要写书评

暂无评论

China Communications 2022年第2期19卷 1-14页

作者： lu jiang Weihua Pei Yijun Wang State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China University of Chinese Academy of Sciences Beijing 100049China Chinese Institute for Brain Research Beijing 102206China

A brain-computer interface(BCI)system based on steady-state visual evoked potentials(SSVEP)was developed by four-class phase-coded *** elicited by flickers at 60Hz,which is higher than the critical fusion frequency(CFF),were compared with those at 15Hz and *** components in electroencephalogram(EEG)were detected using task related component analysis(TRCA)*** analysis with 17 subjects indicated that the highest information transfer rate(ITR)was 29.80±4.65bpm with 0.5s data length for 60Hz and the classification accuracy was 70.07±4.15%.The online BCI system reached an averaged classification accuracy of 87.75±3.50%at 60Hz with 4s,resulting in an ITR of 16.73±*** particular,the maximum ITR for a subject was 80bpm with 0.5s at *** the BCI performance of 60Hz was lower than that of 15Hz and 30Hz,the results of the behavioral test indicated that,with no perception of flicker,the BCI system with 60Hz was more comfortable to use than 15Hz and *** analysis revealed that SSVEP with higher signal-to-noise ratio(SNR)corresponded to better classification performance and the improvement in comfortableness was accompanied by a decrease in *** study demonstrates the feasibility and potential of a user-friendly SSVEP-based BCI using imperceptible flickers.

关键词： brain-computer interface electroencephalogram steady-state visual evoked potentials imperceptible flickers phase coding task related component analysis

Specific function and modulation of teleost monocytes/macrophages: polarization and phagocytosis

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2019年第3期40卷 146-150页

作者： Xin-jiang lu Jiong Chen Laboratory of Biochemistry and Molecular Biology School of Marine Sciences Ningbo University Ningbo Zhejiang 315211 China Key Laboratory of Applied Marine Biotechnology of Ministry of Education Ningbo University Ningbo Zhejiang 315211 China

Macrophages exist in most tissues and play a variety of functions in *** fish species are found in most aquatic environments throughout the world and are quite diverse for a group of vertebrate *** to whole genome duplication and en vironme ntal adaptati on,teleost monocytes/macrophages possess a variety of different functions and modulations compared with those of mammals.A deeper understanding of teleost monocytes/macrophages in the immune system will not only help develop teleost-specific methods of disease prevention but will also help improve our understanding of the various immune mechanisms in *** this review,we summarize the differences in polarizati on and phagocytosis of teleost and mammalian macrophages to improve our understanding of the various immune mechanisms in vertebrates.

关键词： Teleost Monocytes/Macrophages Phagocytosis Cytokine producti on Comparative immunology

Geometric interpretation of several classical iterative methods for linear system of equations and diverse relaxation parameter of the SOR method

在线全文

学校读者我要写书评

暂无评论

Applied Mathematics(A Journal of Chinese Universities) 2013年第3期28卷 269-278页

作者： lu Xing-jiang LEI Lai-i Department of Mathematics Zhejiang University Civil Engineering Laboratory of Macao

Two kinds of iterative methods are designed to solve the linear system of equations, we obtain a new interpretation in terms of a geometric concept. Therefore, we have a better insight into the essence of the iterative methods and provide a reference for further study and design. Finally, a new iterative method is designed named as the diverse relaxation parameter of the SOR method which, in particular, demonstrates the geometric characteristics. Many examples prove that the method is quite effective.

关键词： linear equation iterative method geometric explanation diverse relaxation parameter SORmethod.

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2014年第22期9卷 2002-2007页

作者： Qing Li jiang lu Xianyu Wang Department of Anesthesiology Taihe HospitalHubei University of Medicine Anesthesiology Research Institude Taihe HospitalHubei University of Medicine

Propofol and remifentanil alter intracellular Ca^2＋ concentration （[Ca^2＋]i） in neural stem/progen-itor cells by activating γ-aminobutyric acid type A receptors and by reducing testosterone levels. However, whether this process affects neural stem/progenitor cell proliferation and differenti-ation remains unknown. In the present study, we applied propofol and remifentanil, alone or in combination, at low, moderate or high concentrations （1, 2–2.5 and 4–5 times the clinically effective blood drug concentration）, to neural stem/progenitor cells from the hippocampi of newborn rat pups. Low concentrations of propofol, remifentanil or both had no noticeable effect on cell proliferation or differentiation; however, moderate and high concentrations of propofol and/or remifentanil markedly suppressed neural stem/progenitor cell proliferation and differen-tiation, and induced a decrease in [Ca^2＋]i during the initial stage of neural stem/progenitor cell differentiation. We therefore propose that propofol and remifentanil interfere with the prolifer-ation and differentiation of neural stem/progenitor cells by altering [Ca^2＋]i. Our ifndings suggest that propofol and/or remifentanil should be used with caution in pediatric anesthesia.

关键词： nerve regeneration propofol remifentanil neural stem cells neural progenitor cells proliferation apoptosis differentiation [Ca^2+]i neural regeneration

The role of the gut microbiome in chronic liver diseases:Present insights and future outlook

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2023年第5期22卷 441-443页

作者： lu jiang Jian-Gao Fan Division of Pediatric Gastroenterology and Nutrition Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China Shanghai Key Lab of Pediatric Gastroenterology and Nutrition Shanghai 200092China Shanghai Institute for Pediatric Research Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Gastroenterology Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China

The human gastrointestinal tract harbors trillions of microbes including bacteria,viruses,and fungi that facilitate digestion and nutrient absorption,thereby affecting host metabolism and *** liver communicates with t... 详细信息

关键词： metabolism diseases thereby