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Marker-assisted selection to pyramid Fusarium head blight resistance loci Fhb1 and Fhb2 in the high-quality soft wheat cultivar Yangmai 15

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Journal of Integrative Agriculture 2023年第2期22卷 360-370页

作者： HU Wen-jing FU lu-ping GAO De-rong LI Dong-sheng LIAO Sen lu cheng-bin Key Laboratory of Wheat Biology and Genetic Improvement for Low&Middle Yangtze Valley Ministry of Agriculture and Rural AffairsLixiahe Institute of Agricultural SciencesYangzhou 225007P.R.China College of Agronomy&Center for Crop Genome Engineering Henan Agricultural UniversityZhengzhou 45002P.R.China Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Agricultural College Yangzhou UniversityYangzhou 225009P.R.China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009P.R.China College of Agriculture Yangtze UniversityJingzhou 434023P.R.China

Fusarium head blight(FHB)is one of the most detrimental wheat diseases which greatly decreases the yield and grain quality,especially in the middle and lower reaches of the Yangtze River of ***1 and Fhb2 are two major resistance loci against Fusarium *** 15(YM15)is one of the most popular varieties in the middle and lower reaches of the Yangtze River,and it has good weak gluten characters but poor resistance to *** we used Fhb1 and Fhb2 to improve the FHB resistance of YM15 by a molecular marker-assisted selection(MAS)backcrossing *** selection of agronomic traits was performed for each *** successfully selected seven introgressed lines which carry homozygous Fhb1 and Fhb2 with significantly higher FHB resistance than the recurrent parent *** of the introgressed lines had agronomic and quality characters that were similar to *** study demonstrates that the pyramiding of Fhb1 and Fhb2 could significantly improve the FHB resistance in wheat using the MAS approach.

关键词： Fusarium head blight marker-assisted selection Fhb1 Fhb2 resistance breeding wheat

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Influence of high-molecular-weight glutenin subunit deletions at the Glu-A1 and Glu-D1 loci on protein body development, protein components and dough properties of wheat(Triticum aestivum L.)

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Journal of Integrative Agriculture 2022年第7期21卷 1867-1876页

作者： LIU Da-tong ZHANG Xiao JIANG Wei LI Man WU Xu-jiang GAO De-rong BIE Tong-de lu cheng-bin Key Laboratory of Wheat Biology and Genetic Improvement for Low&Middle Yangtze Valley Ministry of Agriculture and Rural Affairs/Lixiahe Institute of Agricultural Sciences of JiangsuYangzhou 225007P.R.China

High-molecular-weight glutenin subunits(HMW-GSs) play a critical role in determining the viscoelastic properties of wheat. As the organelle where proteins are stored, the development of protein bodies(PBs) reflects the status of protein synthesis and also affects grain quality to a great extent. In this study, with special materials of four near-isogenic lines in a Yangmai 18 background we created, the effects of Glu-A1 and Glu-D1 loci deletions on the development and morphological properties of the protein body, protein components and dough properties were investigated. The results showed that the deletion of the HMW-GS subunit delayed the development process of the PBs, and slowed the increases of volume and area of PBs from 10 days after anthesis(DAA) onwards. In contrast, the areas of PBs at 25 DAA, the middle or late stage of endosperm development, showed no distinguishable differences among the four lines. Compared to the wild type and single null type in Glu-A1, the ratios of HMW-GSs to low-molecular-weight glutenin subunits(LMW-GSs), glutenin macropolymer(GMP) content, mixograph parameters as well as extension parameters decreased in the single null type in Glu-D1 and double null type in Glu-A1 and Glu-D1, while the ratios of gliadins(Gli)/glutenins(Glu) in those types increased. The absence of Glu-D1 subunits decreased both dough strength and extensibility significantly compared to the Glu-A1 deletion type. These results provide a detailed description of the effect of HMW-GS deletion on PBs, protein traits and dough properties, and contribute to the utilization of Glu-D1 deletion germplasm in weak gluten wheat improvement for use in cookies, cakes and southern steamed bread in China and liquor processing.

关键词： wheat near-isogenic lines HMW-GS deletion protein body dough property

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QTL mapping of economically important traits in Silkworm (Bombyx mori)

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Science China(Life Sciences) 2004年第5期47卷 477-484页

作者： lu cheng, LI bin, ZHAO Aichun & XIANG Zhonghuai The Key Sericulture Laboratory of the Ministry of Agriculture of China, College of Sericulture & Biotechnology, Southwest Agricultural University, Chongqing 400716, China 1. The Key Sericulture Laboratory of the Ministry of Agriculture of China College of Sericulture & Biotechnology Southwest Agricultural University 400716 Chongqing China

A backcrossed population (BC1) was derived from a cross between C100 and Dazao. AFLP technique was employed for mapping the QTLs. The QTLs for the whole cocoon weight, cocoon shell weight, ratio of cocoon shell, weight of pupae etc. were analyzed and 11 QTLs were detected based on the constructed linkage map. Two QTLs for whole cocoon weight were local-ized on linkage group 6 and 19; three QTLs for cocoon shell weight were localized on linkage group 3, 14 and 19; three QTLs for ratio of cocoon shell were localized on the linkage group 2, 11 and 15, and three QTLs for the weight of pupae were localized on linkage 2, 14 and 19. All these have laid an important base for the marker assisted breeding of the silkworm.

关键词： Bombyx mori,genetic linkage map,AFLP,QTL,green cocoon.

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Effect of high-molecular-weight glutenin subunit deletion on soft wheat quality properties and sugar-snap cookie quality estimated through near-isogenic lines

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Journal of Integrative Agriculture 2018年第5期17卷 1066-1073页

作者： ZHANG Xiao ZHANG Bo-qiao WU Hong-ya lu cheng-bin Lü Guo-feng LIU Da-tong LI Man JIANG Wei SONG Gui-hua GAO De-rong Key Laboratory of Wheat Biology and Genetic Improvement for Low and Middle Yangtze River Valley Wheat Region Ministry of Agriculture/Yangzhou Academy of Agricultural Sciences

High-molecular-weight glutenin subunits（HMW-GSs） play a critical role in determining the viscoelastic properties of wheat dough. The HMW-GSs are encoded by Glu-A1, Glu-B1, and Glu-D1 loci on the long arms of chromosomes 1A, 1B, and 1D, respectively. In the present study, four near-isogenic lines with different HMW-GS deletions and compositions at the Glu-A1 and Glu-D1 loci in Yangmai 18 background were used for quality analysis. Deletion in Glu-D1 showed much weaker gluten quality and dough strength than null Glu-A1 genotype and wild genotype（WT）, based on the measurements of sodium dodecyl sulfate（SDS）-sedimentation, lactic acid solvent retention capacity（SRC）, gluten index, development time, stability time, and alveograph P and L values. The deletion of Glu-D1 did not significantly affect grain hardness, grain protein content, water SRC, sodium carbonate SRC, and sucrose SRC. Double null genotype in Glu-A1 and Glu-D1 and single null genotype in Glu-D1 showed significantly higher cookie diameter, crispness, and lower cookie height compared with single null genotype in Glu-A1 and WT. These indicate that the null Glu-D1 genotype is useful for improvement of biscuit quality, and use of this germplasm would be a viable strategy to develop new wheat varieties for biscuit processing.

关键词： wheat HMW-GS deletion near-isogenic lines cookie quality

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水泵水轮机热力学法现场效率试验及讨论

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水力发电 2005年第8期31卷 59-60,66页

作者：卢承斌江苏省电力试验研究院江苏南京210036

采用热力学方法进行水泵水轮机现场效率试验,具有许多优点,但也有一定的应用条件。以江苏沙河抽水蓄能电站2号机组的试验为例,在详细介绍IEC41规程中的常规方法的基础上,重点阐述了该次试验时采用的改进方法,并对方法的应用及试验考核... 详细信息

采用热力学方法进行水泵水轮机现场效率试验,具有许多优点,但也有一定的应用条件。以江苏沙河抽水蓄能电站2号机组的试验为例,在详细介绍IEC41规程中的常规方法的基础上,重点阐述了该次试验时采用的改进方法,并对方法的应用及试验考核标准进行了深入探讨。

关键词：水泵水轮机热力学现场效率试验

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Identifying the dynamic behaviors in complete reconstruction of Co-based complex precatalysts during electrocatalytic oxygen evolution

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Journal of Energy Chemistry 2025年第1期100卷 226-233页

作者： Jingfang Zhang Danyang Wu Linke Cai Youluan lu Fanpeng cheng Lijuan Shi Qun Yi Yao Liu Yi Huang Key Laboratory of Green Chemical Engineering Process of Ministry of Education Engineering Research Center of Phosphorus Resources Development and Utilization of Ministry of Education Hubei Key Laboratory of Novel Reactor and Green Chemical Technology School of Chemical Engineering & Pharmacy Wuhan Institute of Technology Key Laboratory of Pesticide & Chemical Biology of Ministry of Education Engineering Research Center of Photoenergy Utilization for Pollution Control and Carbon Reduction of Ministry of Education College of Chemistry Central China Normal University College of Materials Science and Engineering Fuzhou University

Transition metal-based nanomaterials have emerged as promising electrocatalysts for oxygen evolution reaction（OER）. Considerable research efforts have shown that self-reconstruction occurs on these nanomaterials under operating conditions of OER process. However, most of them undergo incomplete reconstruction with limited thickness of reconstruction layer, leading to low component utilization and arduous exploration of real catalytic mechanism. Herein, we identify the dynamic behaviors in complete reconstruction of Co-based complexes during OER. The hollow phytic acid（PA） cross-linked CoFe-based complex nanoboxes with porous nanowalls are designed because of their good electrolyte penetration and mass transport ability, in favor of the fast and complete reconstruction. A series of experiment characterizations demonstrate that the reconstruction process includes the fast substitution of PA by OH-to form Co（Fe）(OH)xand subsequent potential-driven oxidation to Co（Fe）OOH. The obtained CoFeOOH delivers a low overpotential of 290 mV at a current density of 10 mA cm-2and a long-term *** experiment results together with theory calculations reveal that the Fe incorporation can result in the electron rearrangement of reconstructed CoFeOOH and optimization of their electronic structure,accounting for the enhanced OER activity. The work provides new insights into complete reconstruction of metal-based complexes during OER and offers guidelines for rational design of high-performance electrocatalysts.

关键词： Complete reconstruction Co-based complex Precatalysts Oxygen evolution reaction

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某厂600 MW超临界机组真空下降诊断分析

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江苏电机工程 2012年第2期31卷 70-73页

作者：卢承斌江苏方天电力技术有限公司江苏南京211102

针对某厂3台600 MW机组真空下降的问题,通过现场查看,以及对运行历史数据的计算分析,发现凝汽器水阻异常增大,诊断出可能原因,经检查和相关措施,有效改善了真空。进一步分析认为,换热管内泥沙及杂质的沉积,与循泵双速改造后的低速运行有... 详细信息

针对某厂3台600 MW机组真空下降的问题,通过现场查看,以及对运行历史数据的计算分析,发现凝汽器水阻异常增大,诊断出可能原因,经检查和相关措施,有效改善了真空。进一步分析认为,换热管内泥沙及杂质的沉积,与循泵双速改造后的低速运行有关,应引起类似机组的重视。

关键词：真空系统凝汽器诊断分析

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“双创”视域下新媒体课程群建设研究——以湖北第二师范学院为例

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湖北第二师范学院学报 2024年第6期41卷 61-66页

作者：王芬鲁成滨湖北第二师范学院新闻与传播学院武汉430205 江汉大学文学院武汉430056

“双创”视域下,时代与国家对新媒体人才提出了更高的要求。当下短视频直播等新媒体兴起,高校从理论到应用的转型,新媒体课程群建设产学融合,成果导向趋势明显。对此,湖北第二师范学院新闻与传播学院致力打造“产、教、研”三位一体教... 详细信息

“双创”视域下,时代与国家对新媒体人才提出了更高的要求。当下短视频直播等新媒体兴起,高校从理论到应用的转型,新媒体课程群建设产学融合,成果导向趋势明显。对此,湖北第二师范学院新闻与传播学院致力打造“产、教、研”三位一体教学平台,辅以融媒体后台系统,实现科技与课程群深层次联动,为新媒体课程群建设提供参考。

关键词： “双创” 新媒体课程群建设产学融合

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Inhibiting ceramide synthase 5 expression in microglia decreases neuroinflammation after spinal cord injury

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Neural Regeneration Research 2025年第10期20卷 2955-2968页

作者： Wei Zhang Yubao lu Ruoqi Shen Yingjie Wu Chenrui Liu Xingxing Fang Liangming Zhang bin Liu Limin Rong Department of Spine Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina National Medical Products Administration(NMPA)Key Laboratory for Quality Research and Evaluation of Cell Products GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina

Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord ***,the precise mechanism remains *** investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial *** found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high ***,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord ***,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron *** ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced ***,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling *** ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological ***2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling ***,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.

关键词： ceramide synthase 5 gasdermin D microglia neuroinflammation NLRP3 nuclear factor kappa B Pla2g7 pyroptosis sphingomyelin metabolism spinal cord injury

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Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain

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Neural Regeneration Research 2025年第9期20卷 2682-2696页

作者： Xiaoyu Zhang Yuqi Liu Fangxia Xu chengcheng Zhou Kaimei lu bin Fang Lijuan Wang Lina Huang Zifeng Xu Department of Anesthesiology International Peace Maternity and Child Health HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Anesthesiology Shanghai General HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Shanghai Key Laboratory of Embryo Original Diseases ShanghaiChina

Protein arginine methyltransferase-6 participates in a range of biological functions,particularly RNA processing,transcription,chromatin remodeling,and endosomal ***,it remains unclear whether protein arginine methyl transferase-6 modifies neuropathic pain and,if so,what the mechanisms of this *** this study,protein arginine methyltransferase-6 expression levels and its effect on neuropathic pain were investigated in the spared nerve injury model,chronic constriction injury model and bone cancer pain model,using immunohistochemistry,western blotting,immunoprecipitation,and label-free proteomic *** results showed that protein arginine methyltransferase-6 mostly co-localized withβ-tubulinⅢin the dorsal root ganglion,and that its expression decreased following spared nerve injury,chronic constriction injury and bone cancer *** addition,PRMT6 knockout(Prmt6~(-/-))mice exhibited pain ***,the development of spared nerve injury-induced hypersensitivity to mechanical pain was attenuated by blocking the decrease in protein arginine methyltransferase-6 ***,when protein arginine methyltransferase-6 expression was downregulated in the dorsal root ganglion in mice without spared nerve injury,increased levels of phosphorylated extracellular signal-regulated kinases were observed in the ipsilateral dorsal horn,and the response to mechanical stimuli was ***,protein arginine methyltransferase-6 appeared to contribute to spared nerve injury-induced neuropathic pain by regulating the expression of heterogeneous nuclear ***,protein arginine methyltransfe rase-6-mediated modulation of hete rogeneous nuclear ribonucleoprotein-F expression required amino atids 319 to 388,but not classical H3R2 *** findings indicated that protein arginine methyltransferase-6 is a potential therapeutic target fo r the treatment of peripheral neuro pathic pain.

关键词： dorsal root ganglion heterogeneous nuclear ribonucleoprotein F neuropathic pain protein arginine methyltransferase-6 sensory neurons

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