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Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development

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Horticulture Research 2016年第1期3卷 124-133页

作者： Danielle Costenaro da silva Vítor da silveira Falavigna Marianna Fasoli Vanessa Buffon Diogo Denardi Porto Georgios Joannis Pappas Jr Mario Pezzotti Giancarlo Pasquali luís fernando revers Graduate Program in Cell and Molecular Biology Center for BiotechnologyUniversidade Federal do Rio Grande do SulPorto Alegre91501-970RSBrazil Dipartimento di Biotecnologie Universitàdegli Studi di VeronaVerona37134Italy Embrapa Uva e Vinho Bento Gonçalves95701-008RSBrazil Embrapa semiárido Petrolina56302-970PEBrazil Department of Cell Biology Universidade de BrasíliaBrasília70910-900DFBrazil syngenta seeds Ltda UberlândiaMG38407-049Brazil Modesto CA 95354USA

The Dof(DNA-binding with one finger)protein family spans a group of plant transcription factors involved in the regulation of several functions,such as plant responses to stress,hormones and light,phytochrome signaling and seed *** we describe the Dof-like gene family in grapevine(Vitis vinifera L.),which consists of 25 genes coding for *** extensive in silico characterization of the VviDofL gene family was ***,the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray(cv Corvina)and real-time PCR(cv Pinot Noir)*** phylogenetic analysis comparing grapevine sequences with those of Arabidopsis,tomato,poplar and already described Dof genes in other species allowed us to identify several duplicated *** diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological ***,distinct expression patterns were identified between samples analyzed,corroborating such *** expression results indicate that several VviDofL genes perform their functional roles mainly during flower,berry and seed development,highlighting their importance for grapevine growth and *** identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir.

关键词： involvement organs assessed

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Characterization of an Early Berry Development Grapevine somatic Variant (Vitis labrusca L. cv. Isabel Precoce)

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American Journal of Plant sciences 2014年第26期5卷 3848-3865页

作者： Gisele Passaia Márcia Margis-Pinheiro Flavio Bello Fialho Fernanda sbeghen Diogo Denardi Porto luís fernando revers Laboratory of Plant Molecular Biology Genetics Department Federal University of Rio Grande do Sul Porto Alegre Brazil Laboratory of Plant Molecular Genetics Embrapa Grape and Wine Bento Gon&ccedilalves Brazil

Over the past 10 years significant advances have been made towards the description of genetics and molecular mechanisms controlling grapevine berry growth. Regardless of this, many aspects of early fruit morphogenesis and its development control remain to be elucidated. In an attempt to understand gene expression patterns associated with the berry growth development, the contrasting phenotype between the cv. Isabel (Vitis labrusca L.) and its early berry development mutant “Isabel Precoce” has been explored by a candidate gene approach. “Isabel Precoce” (Vitis labrusca L.) was confirmed as an EDV (Essentially Derived Variety) of Isabel, with a 30-35-day reduction in the berry growth phase when compared to the wild type and thus, it constitutes an informative model to investigate many aspects of fruit growth and development. Phenotypic analysis showed that “Isabel Precoce” develops fruits that are smaller in diameter and volume despite of following similar development kinetics. The expression of many genes associated with plant growth and development (MIKCC-type MADs box genes), sugar transport and with the control of flavonoid biosynthetic pathway have been evaluated. The majority of the genes presented a remarkably similar transcription profile. However, a higher induction of transcript accumulation for some genes has been observed in the “Isabel Precoce” genetic background.

关键词： Fruit Development Gene Expression Molecular Biology Grapevine

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Integrated Product and Process Design Using a reverse Engineering system

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厦门大学学报（自然科学版） 2002年第S1期41卷 184-185页

作者： A Ajmal T s Tat school of Engineering South Bank UniversitySchool of Engineering South Bank University UKUK

Computer-integrated manufacturing (CIM) and revers e engineering (RE) have changed drastically the concept of product re-design, pla nning and manufacture of components. However, the main problems currently facing the developers of reverse engineering system, is the time consuming digitis ation of 3D data and the conversion of large amounts of data into a concise and manageable format and linking it to a CAD/CAM system. Automated 3-D profile gen eration, measurements and inspection of manufactured component represents one of the important functions in reverse engineering and in the improvements in produ ct quality in rapid product developments. The paper presents a novel methodology for the development of a reverse enginee ring technique for use in the rapid product development in a CIM environment . The system developed provides integration, data capture and manipulation, dat a transfer between a CAD, CAM, Computer-aided inspection (CAI) and a 3-D profi le scanning system. An efficient scanning strategy has been developed for scann ing and surfaces data acquisition. The products were scanned using a laser scann ing system with a dedicated scan control card and the associated software packag es. A versatile rig was developed for the ease of data gathering of the profile scanning process. The surface data collected was then used to build a mathematic al surface model, which was then used to develop a virtual 3-D model of the pro duct. The resulting surface model provides the geometrical input to the subseque nt machining operation using either a CNC system or other manufacturing operatio n e.g. dies casting/mould casting etc. The prototypes developed were inspected u sing a state-of-the art CNC-CMM that was integrated to the CAD/CAM system. si nce the scanning/digitised data captured by laser scanning probes requires no ma nual editing, significant time saving over most non-contact probe systems was a chieved. since the creation of an accurate CAD model of a redesign

关键词： rapid product development reverse engineering C omputer-Integrated Manufacturing (CIM) re-engineering Co-ordinate Measuring Machines (CMM)

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Novel functional association of rat testicular membrane-associated cytosolic glutathione s transferases and cyclooxygenase in vitro

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Asian Journal of Andrology 2005年第2期7卷 171-178,页

作者： s.Neeraja B.Ramakrishna A.s.sreenath G.V.Reddy P.R.K.Reddy P.Reddanna Department of Animal sciences School of Life SciencesUniversity of HyderabadHyderabad 500046India

Aim:To analyze the role of cytosolic glutathione s-transferases (cGsTs) and membrane-associated cytosolic GsTs (macGsTs) in prostaglandin biosynthesis and to evaluate the possible interaction between glutathione s-transferases (GsTs) and cyclooxygenase (COX) in ***:sDs-PAGE analysis was undertaken for characterization of GsTs,thin layer chromatography (TLC) to monitor the effect of GsTs on prostaglandin biosynthesis from arachi- donic acid (AA) and spectrophotometric assays were done for measuring activity levels of COX and ***: sDs-PAGE analysis indicates that macGsTs have molecular weights in the range of 25-28 *** a coupled assay involving GsTs,arachidonic acid and cyclooxygenase-1,rat testicular macGsTs produced prostaglandin E2 and F2~, while the cGsTs caused the generation of prostaglandin D2,E2 and F_(2α).In vitro interaction studies on GsTs and COX at the protein level have shown dose-dependent inhibition of COX activity by macGsTs and vice *** effect, however,is not seen with *** inhibitory effect of COX on macGsT activity was relieved with increasing concentrations of reduced glutathione (GsH) but not with 1-chloro 2,4-dinitrobenzene (CDNB).The inhibition of COX by macGsTs,on the other hand,was potentiated by ***:We isolated and purified macGsTs and cGsTs from rat testis and analyzed their involvement in prostaglandin *** studies reveal a revers- ible functional interaction between macGsTs and COX in vitro,with possible interactions between them at the GsH binding site of macGsTs.

关键词： glutathione s-transferase cyclooxygenase arachidonic acid glutathione prostaglandins

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彭玉麟与中法战争

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衡阳师范学院学报 2017年第1期38卷 66-69页

作者：鲁风萍华中师范大学历史文化学院湖北武汉430079

中法战争期间彭玉麟前往广东办理防务,先后与两个总督合作。他协助张树声时,由于抗法态度不一,矛盾重重,他提出的很多有效措施未被采纳,广东防务收效甚微。后清政府任命张之洞担任两广总督,彭玉麟向他提出的一系列措施基本上被采纳,并... 详细信息

中法战争期间彭玉麟前往广东办理防务,先后与两个总督合作。他协助张树声时,由于抗法态度不一,矛盾重重,他提出的很多有效措施未被采纳,广东防务收效甚微。后清政府任命张之洞担任两广总督,彭玉麟向他提出的一系列措施基本上被采纳,并得到全力支持。彭、张之间亲密合作,严密设防,广东没有受到法国入侵,保证了地方的安全。但在取得镇南关—谅山大捷、战局发生扭转后,由于清政府采取李鸿章的"避战求和"政策,下令停战撤防,张之洞同意撤防,二者的关系随之发生变化,此时彭玉麟提出的善后措施没有施行。总之,彭玉麟的防务措施不仅与钦、督之间关系有关,还与中枢对法是战是和的态度有着密切的联系。

关键词：中法战争彭玉麟总督防务

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痛风雏鹅肠道菌群通过TLR4/MyD88/NF-κB通路促进肾损伤的研究

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畜牧兽医学报 2021年第2期52卷 543-552页

作者：朱道仙吴植陆江黄涛刘莉江苏农牧科技职业学院动物医学院泰州225300 江苏农牧科技职业学院宠物科技学院泰州225300 江苏省兽用生物制药高技术研究重点实验室泰州225300

旨在研究痛风雏鹅的肠道菌群对肾损伤的影响及作用机制。本研究选取具有典型痛风特征的雏鹅15只,相同日龄及生长环境下的健康雏鹅15只,利用16s rDNA测序技术分析盲肠内容物中菌群的差异;用改良苦味酸法测定血清肌酐(Cr),用酶比色法测定... 详细信息

旨在研究痛风雏鹅的肠道菌群对肾损伤的影响及作用机制。本研究选取具有典型痛风特征的雏鹅15只,相同日龄及生长环境下的健康雏鹅15只,利用16s rDNA测序技术分析盲肠内容物中菌群的差异;用改良苦味酸法测定血清肌酐(Cr),用酶比色法测定血清尿酸(UA),用脲酶-谷氨酸脱氢酶法测定血清尿素氮(BUN);用RT-qPCR和Western blot法测定肾组织Toll样受体4(TLR4)、髓样分化因子(MyD88)、核转录因子-κB(NF-κB)、白细胞介素1β(IL^-1β)、肿瘤坏死因子ɑ(TNF-ɑ)及白细胞介素6(IL-6)的mRNA和蛋白表达量。并将肠道菌群移植给无菌小鼠进行验证。结果显示:1)与健康雏鹅比较,痛风雏鹅肠道菌群16s rDNA测序所得操作分类单元(OTUs)数量、Chao 1指数及shannon指数均显著降低(P<0.01),变形菌门、埃希菌属、变形菌属及肠球菌属等细菌丰度显著增加,痛风雏鹅肠道菌群在免疫系统、细菌感染、膜传输及核苷酸代谢等代谢途径参与度较高;2)与健康雏鹅比较,痛风雏鹅肾组织中TLR4、MyD88、NF-κB、IL^-1β、TNF-ɑ及IL-6的mRNA表达及蛋白表达均显著升高(P<0.05或P<0.01),且这些分子表达量与Cr、BUN及UA呈正相关,与埃希菌属、变形菌属及肠球菌属等菌属丰度呈强正相关关系;3)移植痛风雏鹅肠道菌群小鼠的肾小管发生变性,肾组织TLR4、MyD88、NF-κB的mRNA表达量及血中Cr、BUN、肾损伤因子-1(KIM-1)等水平显著高于移植健康雏鹅菌群的小鼠;给予移植痛风雏鹅菌群小鼠TAK-242后,可回调这些变化。综上表明,痛风雏鹅肠道菌群发生了变化,并可激活LTR4/MyD88/NF-κB通路,诱发炎症反应,促进肾损伤的发生。

关键词：痛风雏鹅肠道菌群 16s rDNA测序 LTR4/MyD88/NF-κB通路肾损伤

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Evaluation of combination gene therapy with PTEN and antisense hTERT for malignant glioma in vitro and xenografts

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南京医科大学学报（自然科学版） 2007年第5期27卷 440-440页

作者： You, Y. P. Geng, X. Z. Zhao, P. FU, Z. Wang, C. Z. Chao, s. W. Liu, N. lu, A. L. Gardner, K. Pu, P. Y. Kong, C. s. Ge, Y. Judge, s. I. V. Li, Q. D. Q Nanjing Med Univ Affiliated Hosp 1 Dept Neurosurg Nanjing 210029 Peoples R China NCI Ctr Canc Res Lab Receptor Biol ＆ Gene Express N1H Bethesda MD 20892 USA. Tianjin Med Univ Gen Hasp Dept Neurosurg Tianjin 300052 Peoples R China Univ Maryland Sch Med VA Maryland Hlth Care Syst MS Ctr Excellence East Baltimore MD 21201 USA Univ Maryland Sch Med Dept Neurol Baltimore MD 21201 USA

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基于微波射频技术的三维智能雷达倒车系统设计

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通化师范学院学报 2020年第2期41卷 1-4页

作者：刘皖苏卢彪宿州学院信息工程学院安徽宿州234000 宿州学院安徽宿州234000

为了解决传统二维雷达倒车系统的缺点,设计了基于微波射频技术的智能三维倒车雷达系统.首先设计了雷达系统的总体框架、各类功能模块的具体功能和工作原理.然后,进行了系统硬件模块设计.包括微波射频水平测距模块、地面测距模块、温度... 详细信息

为了解决传统二维雷达倒车系统的缺点,设计了基于微波射频技术的智能三维倒车雷达系统.首先设计了雷达系统的总体框架、各类功能模块的具体功能和工作原理.然后,进行了系统硬件模块设计.包括微波射频水平测距模块、地面测距模块、温度补偿模块和智能语音警报模块.最后,进行了系统软件设计,实现了智能三维倒车雷达系统的具体功能.结合所设计的温度补偿算法对系统进行精确测试,系统可实现水平精确测距、地面凸起或凹坑的精准识别、语音警报和LCD屏上实时显示水平与地面监测距离.

关键词：微波射频温度补偿智能三维语音警示

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人端粒酶逆转录酶永生化MRC-5细胞方法的建立

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中华实验和临床病毒学杂志 2015年第2期29卷 177-179页

作者：吴萌殷建忠朱武洋鲁茁壮昆明医科大学公共卫生学院昆明650500 中国疾病预防控制中心病毒病所

目的通过携带人端粒酶逆转录酶（hTERT）的慢病毒颗粒转染法,制备可用于人巨细胞病毒（HCMV）培养的永生化MRC-5细胞.方法以携带hTERT基因的质粒pLXsN-hTERT为分子基础,通过PCR构建慢病毒载体质粒pLVX-EFl ***-EF1 a-hTERT质粒与Lenti... 详细信息

目的通过携带人端粒酶逆转录酶（hTERT）的慢病毒颗粒转染法,制备可用于人巨细胞病毒（HCMV）培养的永生化MRC-5细胞.方法以携带hTERT基因的质粒pLXsN-hTERT为分子基础,通过PCR构建慢病毒载体质粒pLVX-EFl ***-EF1 a-hTERT质粒与Lenti-X HTXPackaging Mix2混合后转染293T细胞,并经Real-time PCR定量所获得的慢病毒颗粒,在慢病毒原液感染MRC-5细胞后连续传代获得永生化的MRC-5T细胞,并观察HCMV在MRC-5T细胞中的感染特性.结果获得了表达hTERT基因的慢病毒颗粒LVX-EF1a-hTERT,制备了可用于HCMV培养的永生化MRC-5细胞.结论建立了人端粒酶逆转录酶永生化MRC-5细胞的方法,为永生化原代细胞等其他种类细胞提供了技术保障和物质基础.

关键词：细胞培养的巨细胞病毒 HIV-1逆转录酶

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APPLICATION OF GREY sYsTEM THEORY TO PROCEssING OF MEAsURING DATA IN reversE ENGINEERING

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Transactions of Nanjing University of Aeronautics and Astronautics 2003年第1期20卷 36-41页

作者：平雪良周儒荣安鲁陵南京航空航天大学CAD/CAM工程研究中心

The processing of measuri ng data plays an important role in reverse engineering. Based on grey system the ory, we first propose some methods to the processing of measuring data in revers e engineering. The measured data usually have some abnormalities. When the abnor mal data are eliminated by filtering, blanks are created. The grey generation an d GM(1,1) are used to create new data for these blanks. For the uneven data sequ en ce created by measuring error, the mean generation is used to smooth it and then the stepwise and smooth generations are used to improve the data sequence.

关键词： reverse engineering gr ey system theory digitization data processing grey generation

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