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Discovery of mitochondrial chimeric-gene associated with cytoplasmic male sterility of HL-rice

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Chinese Science Bulletin 2002年第9期47卷 744-747页

作者： yl Ping WANG Li SUN Qingping ZHU Yingguo The Key Laboratory of Ministry of Education for Plant Developmental Biology and Institute of Genetics College of Life SciencesWuhan UniversityWuhan 430072China

The mitochondrial genome libraries of HL-type sterile line(A) and maintainer line(B) have been constructed. Mitochondrial gene, atp6, was used to screen libraries, due to the different Southern and Northern blot results between sterile and maintainer line. Sequencing analysis of positive clones proved that there were two copies of atp6 gene in sterile line and only one in maintainer line. One copy of atpt6 in sterile line was same to that in maintainer line; the other showed different flanking sequence from the 49th nucleotide downstream of the termination codon of atp6 gene. A new chimeric gene, orfH79, was found in the region. OrfH79 had homology to mitochondrial gene cox Ⅱ and orf107, and was special to HL-sterile cytoplasm.

关键词： cytoplasmic male sterility mitochondria chimeric gene.

An Efficient and Convenient Cyclization Method for the Synthesis of Flavans

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Chinese Chemical Letters 1998年第9期9卷 805-807页

作者： Hou, LM Huang, CS Cen, W Li, Y Li, yl Lanzhou Univ Inst Organ Chem Lanzhou 730000 Peoples R China Lanzhou Univ Natl Lab Appl Organ Chem Lanzhou 730000 Peoples R China Qingdao Univ Dept Chem Qingdao 266071 Peoples R China

A convenient cyclization method for the flavans synthesis is described. BF3 was used for the first time as an efficient catalyst to effect the cyclization of 1,3-diarylpropan-1-ols.

关键词： flavans cyclization synthesis

Fe/Cu gradient composite materials prepared by laser sintering

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Chinese Science Bulletin 1997年第23期42卷 2014-2016页

作者： Li, yl Guan, QF Wang, CS Hu, JD Jilin Univ Technol Dept Met Mat Engn Changchun 130025 Peoples R China

USLNG CO2 laser beam as a heat source to sinter whole P/M （powder metallurgy） green com-pact is a new technique. As reported in refs. [1--4], the advantages of the laser sintering ofCu-base and Fe-base green compact... 详细信息

关键词： Cu Fe/Cu gradient composite materials prepared by laser sintering

压电型机械门控离子通道组件1在大鼠压力性损伤中的作用机制

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中国组织工程研究 2025年第8期29卷 1578-1584页

作者：孙家琪卞璐史文涛吴学潮鲁晓杰江南大学附属中心医院神经外科江苏省无锡市214026 无锡市神经外科研究所江苏省无锡市214026

背景:压力性损伤的发生机制复杂,哪些因素在其发生中起到核心作用,这些因素具体又是如何发生的尚不完全清楚。目的:探讨压电型机械门控离子通道组件1(piezo-type mechanosensitive ion channel component 1,PIEZO1)与压力性损伤发生的... 详细信息

背景:压力性损伤的发生机制复杂,哪些因素在其发生中起到核心作用,这些因素具体又是如何发生的尚不完全清楚。目的:探讨压电型机械门控离子通道组件1(piezo-type mechanosensitive ion channel component 1,PIEZO1)与压力性损伤发生的关系。方法:①细胞实验:采用不同浓度的PIEZO1激动剂Yoda1干预人永生化角质形成细胞(HaCaT),检测细胞活性、钙离子内流及PIEZO1、凋亡相关蛋白的表达。②动物实验:采用随机数字表法将12只SD大鼠随机分为4组,每组3只:对照组大鼠不进行任何处理,1,2,3 mm组分别采用厚度1,2,3 mm的磁铁在大鼠背部两侧压迫1 h,建立压力性损伤模型,造模后切除全部创面组织,分别进行苏木精-伊红、Masson、免疫荧光染色及Western blot检测。结果与结论:①细胞实验:活/死细胞染色结果显示,随着Yoda1浓度(0,2.5,5,10μmol/L)的升高,HaCaT细胞凋亡增加;随着Yoda1浓度(0,5,10μmol/L)的升高,HaCaT细胞钙离子内流增加,并且随着处理时间的延长,钙离子内流增多;Western blot检测结果显示,与对照组(0μmol/L Yoda1干预)比较,5,10μmol/L Yoda1干预可提升HaCaT细胞中BAX、TG2、PIEZO1蛋白的表达,降低Bcl-2蛋白的表达;②动物实验:苏木精-伊红与Masson染色结果显示,3个实验组压迫部位皮肤结构被破坏,皮下脂肪液化坏死,胶原蛋白稀疏且排列紊乱,并且随着磁铁厚度(压力)的增加,压迫部位皮肤组织结构破坏程度加重;免疫荧光染色与Western blot检测结果显示,与对照组比较,3个实验组大鼠BAX、TG2、Yap1与PIEZO1蛋白表达升高,Bcl-2蛋白表达降低,并且随着磁铁厚度(压力)的增加,相关蛋白表达值改变更加明显;③结果表明:皮肤受压会激活PIEZO1使钙离子大量内流,随着所受压力的不断增加,最终导致钙超载后的细胞凋亡。

关键词：压力性损伤压电型机械门控离子通道组件1 PIEZO1 钙超载 HaCaT细胞

基于FLAC^(3D)的高抽巷围岩稳定性数值模拟分析

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煤矿现代化 2025年第1期34卷 52-57,65页

作者：芦盛亮山西潞安化工集团余吾煤业有限责任公司山西屯留046100

为提高高抽巷的稳定性,本文采用数值模拟的手段分析了S1302高抽巷在开挖过程中的支承压力分布规律,采用分段掘进的方法共计掘进了3次。研究结果表明,高抽巷在开挖过程中,左右两侧的侧向支承压力分布特征并不相同。在靠近实体煤一侧,侧... 详细信息

为提高高抽巷的稳定性,本文采用数值模拟的手段分析了S1302高抽巷在开挖过程中的支承压力分布规律,采用分段掘进的方法共计掘进了3次。研究结果表明,高抽巷在开挖过程中,左右两侧的侧向支承压力分布特征并不相同。在靠近实体煤一侧,侧向支承压力随距离增加逐渐缩小。在靠近回风平巷一侧,侧向支承压力随距离增加迅速下降,当超过S1302回风平巷影响范围后,侧向支承压力逐渐增加至原岩应力,然后保持不变。

关键词：高抽巷数值模拟支承压力原岩应力

Atemoyacin A: A new bis-tetrahydrofuranyl annonaceous acetogenin from Annona atemoya H.

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Chinese Journal of Chemistry 1995年第3期13卷 263-266页

作者： CHEN, WS YAO, ZJ ZHANG, YB XU, YZ WU, yl CHINESE ACAD SCI SHANGHAI INST ORGAN CHEMSTATE KEY LAB BIOORGAN & NAT PROD CHEMSHANGHAI 20032PEOPLES R CHINA CHINESE ACAD SCI S CHINA INST BOTCANTON 510650PEOPLES R CHINA

Atemoyacin A, a new annonaceous acetogenin was isolated from the alcoholic extract of the seeds of Annona atemoya Hort (Annonaceae). Its structure and relative configuration were elucidated on the basis of the spectra... 详细信息

关键词： ANONNA ATEMOYA HORT ANNONACEOUS ACETOGENIN ATEMOYACIN A STRUCTURAL EluCIDATION RELATIVE CONFIGURATION

Expression and anti-apoptotic mechanism of Spodoptera littoralis Nucleopolyhedrovirus P49 protein

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Chinese Science Bulletin 2002年第8期47卷 668-671页

作者： Pei, ZF Qi, YP Liu, yl Xiao, HZ Wuhan Univ Inst Virol Wuhan 430072 Peoples R China

A novel cloned Spodoptera littoralis Nucleo-polyhedrovirus (SINPV) p49 gene is able to suppress apop-tosis of insect cells Sf9 triggered by virus. The amino acid sequence of P49 expressed in baculovirus expression system is the same as predicted, indicating that the expression of P49 is correct. Metabolic labeling revealed that p49 was able to be expressed both in the early and late phases after the viral infection, and only in the late phase was the expression driven by polyhedra promoter, but the amount of expression was higher than that of wtSlNPV. In summary, the early gene of SINPV p49 as well as p35 of AcMNPV is able to be expressed in the late phase, but its promoter is weaker compared with polyhedra promoter. In vitro, P49 can be cut by Bm caspase and human caspase-3, yielding 10 and 40 ku fragments. Purified P49 blocks the substrate cleavage by Bm caspase and human caspase-3, showing that P49 inhibits downstream caspases in the apoptotic pathway.

关键词： apoptosis p49 gene Caspase.

EPOXIDATION　OF　OLEFINS　WITH　IODOSylBENZENE　CATALYZED　BY　POLYMER－SUPPORTED　MANGANESE　PORPHYRINS

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Chinese Chemical Letters 1996年第4期7卷 329-332页

作者： You, JS Yu, XQ Xiao, YF Wu, yl Lan, ZW SICHUAN UNIV DEPT CHEMCHENGDU 610064PEOPLES R CHINA

Polymer- bound manganese (Ⅲ) porphyrins are effective catalysts for the epoxidation of olefins by iedosylbenzene.

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The osmium isotopic compositions of Cenozoic basalts and peridotite xenoliths from Anhui and Jiangsu provinces

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Chinese Science Bulletin 1997年第7期42卷 577-580页

作者： Zhi, XC Zheng, YF Du, AD Sun, yl Department of Earth and Space Sciences University of Science and Technology of China Hefei China National Research Center of Geoanalysis Beijing China

THE determination of contents of trace (10-12—10-9) siderophile elements, Re and Os, and osmium isotopic compositions in mantle-derived rocks is very useful. It has been a new approach to exploring interactions between the mantle and the crust, and the chemical structure of the mantle. This can be done by combination with isotopic dating and tracing

关键词： osmium isotope spinel Iherzolite continental basalt mantle.