检索结果-南通市图书馆

Recommendation letter for Asian pacific Journal of Tropical Biomedicine(APJTB)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2013年第6期3卷 421-421页

作者： Alcides troncoso Department of Microbiology and Infections Discases School of MedicineBuenos Aires University

Our world has demonstrated to he extremely *** humanitarian missions in developing countries have revealed the monstrous devastation caused by tropical diseases and ***,the people in charge of the political decisions always seem to be far from recognizing this situation when facing the monstrous dose of reality of the transmissible diseases that have predilection for the people in disadvantage.

关键词： APJTB Recommendation letter for Asian pacific Journal of Tropical Biomedicine

Enhancement of the benthic communities around an isolated island in the Antarctic Ocean

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2013年第6期32卷 47-55页

作者： José I Saiz Nuria Anadón Javier Cristobo Oscar García-Alvarez Gerardo García-Castrillo Eduardo López Cruz Palacín Jesús S troncoso Ana Ramos Department of Zoology and Animal Cell Biology University of the Basque CountryBilbao 48080 Spain Department of Organisms and Systems Biology (Zoology) Rodrigo Uría s/n University of OviedoOviedo 33071 Spain Spanish Institute of Oceanography Gijón Oceanographic Centre Príncipe de Asturias 70bisGijón 33212 Spain Department of Zoology and Physical Anthropology University of Santiago de CompostelaSouth Campus Santiago de Compostela 15706 Spain Cantabrian Maritime Museum San Martín de Bajamar s/nSantander 39004 Spain Department of Biology Faculty of Sciences Autonomous University of MadridMadrid 28049 Spain Department of Animal Biology University of BarcelonaAvinguda Diagonal 645 Barcelona 08028 Spain Department of Ecology and Animal Biology Marine Sciences Faculty University of VigoVigo 36310 Spain Spanish Institute of Oceanography Subida a Radio Faro 50-52Vigo 36390 Spain

Insular marine biotas are often richer in faunal diversity than those from the open sea in the same geo- graphical region. The existence of particular island effects were tested under polar conditions by compar- ing infaunal benthic assemblages on Peter I Island with those of similar latitudes in the open ocean at the Bellingshausen Sea and also from the coast of the Antarctic Peninsula. Sampling was carried out aboard the R/V Hesperides during the expedition named BENTART-2003 from 24 January to 3 March 2003. Benthic samples were collected at 18 stations ranging from 90 to 2 044 m depth, using an USNEL-type box corer （BC） dredge. Representatives of 32 higher taxa of invertebrates were found. Univariate and multivariate analyses revealed various patterns in the data. First, significant differences in polychaete abundance were detected between the stations located in the open sea and the remaining sites （island plus mainland sites）. Bivalve abundances were also distinct between island and mainland sampling sites. Sediment columns taken from the island seafloor exhibited the highest rate of bioturbation by the infauna. These findings confirm the model that islands develop distinct assemblages characterized by the enhancement of the benthic commu- nities even in cold waters. Several abiotic factors were measured simultaneously at the seafloor and along the water column to investigate faunal distribution patterns. Significant correlations were found between the benthic assemblages and a combination of two environmental variables：＂island effect＂（measured as a categorical variable） and the redox state of sediments. Richer and more complex benthic assemblages were found in Peter I Island＇s sea bottom, whereas the more depauperate bottoms remained in the open sea.

关键词： Antarctica Bellingshausen Sea benthos Peter I Island PRIMER

Diagnosis of Mycobacterium tuberculosis using molecular biology technology

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2011年第2期1卷 89-93页

作者： Juan Garberi Jorge Labrador Federico Garberi Juan Ezequiel Garberi Julian Peneipil Miguel Garberi Luis Scigliano Alcides troncoso Laboratory of Molecular Biology and Pathology School of MedicineBuenos Aires University Orange Solutions School of MedicineBuenos Aires University Department of Infectious Diseases School of MedicineBuenos Aires University

Objective:To present an integrated molecular biology dedicated system for tuberculosis ***:One hundred and five sputum specimens from patients strongly suspected by clinical parameters of tuberculosis were studied by Ziehl-Neelsen staining,by cultivation on solid medium and by a balanced hemincsted fluorometric PCR system(Orange C3TB) that could preserve worker safety and produce a rather pure material free of potential inhibitors. DNA amplification was performed in a low cost tuberculosis *** double stranded DNA was flurometrically *** whole reaction was conducted in one single tube which would not be opened after adding the processed sample in order to minimize the risk of cross contamination with ***:The assay was able to delect 30 bacillus per sample mL with 99.8%interassay variation *** was positive in 23(21.9%) tested samples(21 of them were smear negative).In our study it showed a preliminary sensitivity of 94.5%for sputum and an overall specificity of 98.7%.Conclusions:Total run time of the test is 4 h with 2.5 real working *** PCR positive samples are also positive by microbiological culture and clinical *** show that it could be a very useful tool to increase detection efficiency of tuberculosis disease in low bacilus load ***,its low cost and friendly using make it feasible to run in poor regions.

关键词： Tuberculosis Sputum Molecular diagnosis Low cost Real-time PCR Mycobacterium tuberculosis Molecular biology technology Sputum sample Microbiological culture

MicroRNAs contribute to ATP-binding cassette transporter-and autophagy-mediated chemoresistance in hepatocellular carcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Hepatology 2019年第4期11卷 344-358页

作者： María V Espelt María L Bacigalupo Pablo Carabias María F troncoso Facultad de Farmacia y Bioquímica Instituto de Química y Fisicoquímica Biológicas Consejo Nacional de Investigaciones Científicas y Técnicas Universidad de Buenos Aires

Hepatocellular carcinoma(HCC) has an elevated mortality rate, largely because of high recurrence and metastasis. Additionally, the main obstacle during treatment of HCC is that patients usually develop resistance to *** drug resistance involves many different mechanisms, including alterations in drug metabolism and processing, impairment of the apoptotic machine, activation of cell survival signaling, decreased drug sensitivity and autophagy, among others. Nowadays, miRNAs are emerging as master regulators of normal physiology-and tumor-related gene expression. In HCC,aberrant expression of many miRNAs leads to chemoresistance. Herein, we particularly analyzed miRNA impact on HCC resistance to drug therapy. Certain miRNAs target ABC(ATP-binding cassette) transporter genes. As most of these miRNAs are downregulated in HCC, transporter levels increase and intracellular drug accumulation decrease, turning cells less sensitive to death. Others miRNAs target autophagy-related gene expression, inhibiting autophagy and acting as tumor suppressors. Nevertheless, due to its downregulation in HCC, these miRNAs do not inhibit autophagy or tumor growth and, resistance is ***, modulation of ABC transporter and/or autophagy-related gene expression or function by miRNAs could be determinant for HCC cell survival under chemotherapeutic drug treatment. Undoubtedly, more insights on the biological processes, signaling pathways and/or molecular mechanisms regulated by miRNAs are needed. Anyway, miRNA-based therapy together with conventional chemotherapeutic drugs has a great future in cancer therapy.

关键词： Hepatocellular carcinoma Chemoresistance ABC transporter Autophagy miRNA

Induction of Defensive Responses in Eucalyptus globulus(Labill) Plants, against Ctenarytaina eucalypti(Maskell) (Hemiptera: Psyllidae)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2012年第5期3卷 589-595页

作者： Christian troncoso Jose Becerra Claudia Perez Victor Hernandez Aurelio San Martin Manuel Sanchez-Olate Darcy Rios Centro de Biotecnologia Laboratorio de Cultivo de Tejidos Vegetales Universidad de Concepcion Concepcion Chile Departamento de Botanica Facultad de Ciencias Naturales y Oceanograficas Universidad de Concepcion Concepcion Chile Departamento de Quimica Facultad de Ciencias Universidad de Chile Santiago Chile Facultad de Ciencias Forestales Universidad de Concepcion Concepcion Chile

This study evaluated the expression of defense compounds from the secondary metabolism of Eucalyptus globulus plants, subjected to direct and indirect stimuli by the insect Ctenarytaina eucalypti (blue gum Psyllid). Results showed that defense responses were activated in plants in all tested cases. Were detected and identified thirty-two compounds in the leaves of treated plants, of which five compounds differed with the control, and all are part of the chemical defenses from the plants, three of them were oxygenated monoterpenes (borneol, exo-2-hydroxy cineole and thymol), a aromatic carboxylic acid (benzoic acid) and a quinone (6-acethyl-flaviolin). The plants induced by volatile compounds and by indirect entomological manner, showed its capability to synthesize defensive compounds without a wound that promotes these responses. Were also found some constitutive secondary metabolites over expressed in the different inductions compared with the control.

关键词： Chemical Defense E. globules Secondary Metabolites Terpenes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

定向射孔在底水稠油油藏开发中的应用

国外测井技术 2008年第2期23卷 27-29页

作者：韩洪宝倪学锋 Juan troncoso 中石化石油勘探开发有限公司北京100031 Repsol Ecuador S．A．Ecuador·Quito

G-Force^TM系统是哈里伯顿公司新推出的一项在任何方向精确定向射孔的技术,通过在南美厄瓜多尔奥联特盆地强边底水稠油油藏大斜度定向井的实际应用,定向射孔新技术延缓了底水锥进速度,达到控水稳油的目的,大大提高了底水稠油油藏原油的... 详细信息

G-Force^TM系统是哈里伯顿公司新推出的一项在任何方向精确定向射孔的技术,通过在南美厄瓜多尔奥联特盆地强边底水稠油油藏大斜度定向井的实际应用,定向射孔新技术延缓了底水锥进速度,达到控水稳油的目的,大大提高了底水稠油油藏原油的最终采收率。

关键词： G-Force^TM 定向射孔底水稠油油藏稳油控水提高采收率

Cryopreservation by Vitrification of Vitis vinifera cv. ＂Red Globe＂ Zygotic Embryos and Effect on the Expression of DNA Methyltransferase Genes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Agricultural Science and Technology(B) 2016年第6期6卷 387-399页

作者： Heriberto Garcia-Coronado Maria-Elena Baez-Flores Rosalba troncoso-Rojas Marisela Rivera-Dominguez Martin-Emesto Tiznado-Hernandez Coordinacion de Tecnologia de Alimentos de Origen Vegetal Centro de Investigacion en Alimentacion y Desarrollo A.C. (CLAD)Hermosillo Sonora CP 83304 Mexico Facultad de Ciencias Quimico Biol6gicas Universidad Autonoma de Sinaloa Culiacan Sinaloa CP 80013 Mexico Coordinacion de Ciencia de los Alimentos Centro de Investigacion en Alimentacion y Desarrollo A.C. (CLAD) HermosilloSonora CP 83304 Mexico

Mexico is a large producer of table grape （Vitis vinifera L.） and therefore it is important to develop protocols to store the grape varieties germplasm. The objective of the present work was to design a protocol for the cryopreservation by vitrification of zygotic embryos of V. vinifera cv. ＂Red Globe＂ and evaluate possible epigenetics changes. The plant vitrification solution 2 （PVS2） was utilized before the utilization of liquid nitrogen （LN）. The effect of this protocol on embryo viability was tested by the triphenyl-tetrazolium chloride solution, as well as by the in vitro development of grape embryos into plantlet. A cDNA expression library of grape zygotic embryos was created to isolate expressed sequence tags of several DNA methyltrasferases. Gene expression of domains rearranged methyltransferase type 1 （DMR1） and DNA （cytosine-5）-methyltransferase 1 （MET1-2） isozymes was analyzed by quantitative reverse transcriptase PCR. The optimal conditions for vitrification were 10 min in 50% PVS2, followed by I0 min in 100% PVS2. Under these conditions, about 30% of plantlet was obtained from embryos after cryopreservation. It was recorded a reduction in the MET1-2 gene expression, which plays a role in the maintenance of DNA methylation. It is possible to cryopreserve viable grape zygotic embryos, although the treatment seems to induce alterations in the normal DNA methylation pattern of the zygotic embryo genome.

关键词： Vitis vinifera zygotic embryo cryopreservation vitrification DNA methyltransferase.

The Effect of UV Light and CO2in the Production of Polyunsaturated Aldehydes in Skeletonema costatum(Bacillariophycea)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2014年第24期5卷 3632-3641页

作者： Luiz Felipe C. da Motta Pacheco Eduardo Uribe Jorge Pino José troncoso Andrés Quiróz Laboratório de Producción Primaria Facultad de Cienciasdel Mar Universidad Católica del Norte Coquimbo Chile EWOS Innovation Bergen Norway Laboratório de Química Ecológica Instituto de Química Universidad de La Frontera Temuco Chile

Skeletonema costatum was submitted to two experiments using UV lights and CO2with the purpose of observing changes in the lipids profile and the synthesis of polyunsaturated aldehydes (PUA) after cell disruption. When cells receive CO2 supply, it was noticed that the production of PUA was significantly lower. The same was observed when the culture was treated with a dose of 45.9 kJ·m-2 of ultra-violet A/B ray. The premise to all experiments was the production of 2,4-heptadienal compared to the supply of EPA as substrate. As a result, the same synthesis rate was observed both when the CO2 treatment was applied and in the experiment control. On the other hand, the culture subjected to ultraviolet radiation showed a 68% greater demand with the utilization of the substrate. These observations suggested that EPA was consumed before cell disruption and was probably exuded to the surrounding environment as a sign of stress. Changes in cell morphology could be observed by the migration of the chloroplast nearby the cell wall, where PUA was produced, indicating a defense strategy.

关键词： Diatom Physiology Oxilipins Skeletonema costatum Polyunsaturated Aldehyde (PUA)

Synergism Effect between Phenolic Metabolites and Endogenous Antioxidants in Terms of Antioxidant Activity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Chemical Engineering and Science 2014年第2期4卷 258-265页

作者： María Noguer Ana B. Cerezo M. Luisa Moyá Ana M. troncoso M. Carmen García-Parrilla Nutrición y Bromatología Facultad de Farmacia Universidad de Sevilla Sevilla Spain

Polyphenolic compounds, widely distributed in plant kingdom, have been exhaustively studied for their bioactive properties specially antioxidant activity. However, they are extensively metabolized by human organism and the resulting metabolites are largely responsible for their effects. Furthermore, they may interact with the endogenous antioxidant network being this possibility scarcely studied. Plasma antioxidant network encompasses antioxidant enzymes and other substances such as uric acid. In addition, ascorbic acid is the major compound representing water soluble compartment both in foods and human body. The interaction of this vitamin with phenolic compound is largely unexplored. This work aims to study if there is a synergic effect between phenolic metabolites and main antioxidants (uric and ascorbic acid). For this purpose, the antioxidant activity was evaluated in terms of ORAC (oxygen radical absorbance capacity) and FRAP (ferricreducing antioxidant power) as these tests involved either HAT (Hydrogen Atom Transfer) or SET (Single Electron Transfer) mechanisms. Additionally, a kinetic studied was developed to test if the rate constant presented a synergic effect. Protocatechuic acid, 3,4-dihydroxyphenylacetic acid, 3,4-dihydroxyphenylpropionic acid and 3-hydroxyphenylacetic acid were selected as they were metabolites of polyphenol compounds such as anthocyanins, quercetin, neohesperidin, chlorogenic acid and hesperetin present in wines, orange and strawberries. A synergic effect was proved for the combination of ascorbic acid with 3,4-dihydroxyphenylacetic acid, both in terms of antioxidant activity and potent increase of velocity of the antioxidant reaction that took place.

关键词： Kinetics Ascorbic Acid Uric Acid ORAC FRAP