检索结果-南通市图书馆

Allosteric DNAzyme-based encoder for molecular information transfer

Chinese Chemical Letters 2024年第7期35卷 235-241页

作者： Minghao Hu Tianci Xie Yuqiang Hu longjie li Ting Wang Tongbo Wu School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Oncology Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China GI Cancer Research Institute Tongji HospitalHuazhong University of Science and TechnologyWuhan 430030China School of life Science and Technology Wuhan Polytechnic UniversityWuhan 430023China State Key Laboratory of Bioelectronics National Demonstration Center for Experimental Biomedical Engineering EducationSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China

Dynamic DNA nanotechnology plays a significant role in nanomedicine and information science due to its high programmability based on Watson-Crick base pairing and nanoscale *** DNA machines and networks have been widely used in various fields,including molecular imaging,biosensors,drug delivery,information processing,and logic *** serve as crucial components for information compilation and transfer,allowing the conversion of information from diverse application scenarios into a format recognized and applied by DNA ***,there are only a few encoder designs with DNA ***,the molecular priority encoder is hardly *** this study,we introduce allosteric DNAzyme-based encoders for information *** design of the allosteric domain and the recognition arm allows the input and output to be independent of each other and freely *** pre-packaged mode design achieves uniformity of baseline dynamics and dynamics *** also integrated non-nucleic acid molecules into the encoder through the aptamer design of the allosteric ***,we developed the 2^(n)-n encoder and the EndoⅣ-assisted priority encoder inspired by immunoglobulin's molecular structure and effector *** our knowledge,the proposed encoder is the first enzyme-free DNA encoder with DNA output,and the priority encoder is the first molecular priority encoder in the DNA reaction *** encoders avoid complex operations on a single molecule,and their simple structure facilitates their application in complex DNA circuits and biological scenarios.

关键词： DNAzyme Encoder Nucleic acids DNA circuit DNA strand displacement Dynamic DNA nanotechnology

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex

引用

Chinese Chemical Letters 2024年第5期35卷 330-337页

作者： Yang Qin Jiangtian li Xuehao Zhang Kaixuan Wan Heao Zhang Feiyang Huang limei Wang Hongxun Wang longjie li Xianjin Xiao Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pancreatic Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Wuhan Polytechnic University Wuhan 430023China

Strand displacement reaction is a crucial component in the assembly of diverse DNA-based nanodevices,with the toehold-mediated strand displacement reaction representing the prevailing ***,the single-stranded Watson-Crick sticky region that serves as the trigger for strand displacement can also cause leakage reactions by introducing crosstalk in complex DNA ***,we proposed the toeless and reversible DNA strand displacement reaction based on the Hoogsteen-bond triplex,which is compatible with most of the existing DNA *** demonstrated that our proposed reaction can occur at pH 5 and can be reversed at pH *** also observed an approximately linear relationship between the degree of reaction and pH within the range of pH 5-6,providing the potential for precise regulation of the ***,by altering the sequence orientation,we have demonstrated that our proposed reaction can be initiated or regulated through the same toeless mechanism without the requirement for protonation in low pH *** on the proposed reaction principle,we further constructed a variety of DNA nanodevices,including two types of DNA logic gates that rely on pH 5/pH 9 changes for initiating and reversing:the AND gate and the OR *** also successfully constructed a DNA Walker based on our proposed reaction modes,which can move along a given track after the introduction of a programmable DNA sequence and complete a cycle after 4 *** findings suggest that this innovative approach will have broad utility in the development of DNA circuits,molecular sensors,and other complex biological systems.

关键词： DNA strand displacement Toeless Triplex DNA strands Reversible DNA circuit DNA walker

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

空间约束下电动汽车无线充电系统磁耦合结构优化

引用

电工技术学报 2024年第12期39卷 3581-3588页

作者：徐先峰吴慧玲杨雄政卢勇李陇杰长安大学能源与电气工程学院西安710064

电动汽车无线充电系统在尺寸大小与位置偏移等因素的影响下,会导致系统传输能力下降。为了在空间约束条件下提升系统的输出功率、传输效率及抗偏移能力,针对位置偏移与底盘高度不同的实际情况,在经典D4线圈的基础上分别设计了收发端非对... 详细信息

电动汽车无线充电系统在尺寸大小与位置偏移等因素的影响下,会导致系统传输能力下降。为了在空间约束条件下提升系统的输出功率、传输效率及抗偏移能力,针对位置偏移与底盘高度不同的实际情况,在经典D4线圈的基础上分别设计了收发端非对称D4和收发端非对称D4Q双层线圈磁耦合结构,以提高耦合系数和抗偏移能力。在不同方向的偏移和旋转的情况进行仿真测试,表明收发端非对称D4Q双层线圈在出现横向偏移300 mm、纵向偏移400 mm、传输距离230 mm和旋转偏移45°以内具备无线电能传输能力。通过搭建实验平台对偏移进行测试与验证,结果表明,所提出的收发端非对称D4Q磁耦合结构的最大输出功率相对于收发端对称D4磁耦合结构增加约60.34%,最大传输效率增大11%。

关键词：电动汽车无线充电补偿电路拓扑磁耦合结构平台搭建

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Triplex-structure based DNA circuits with ultra-low leakage and high signal-to-noise ratio

引用

Chinese Chemical Letters 2024年第1期35卷 465-470页

作者： Huan liu Zhihao Ming Yuanpeng Zhang Qidong Xia Hao Hu Ruijie liu Yuheng liao Yizhou liu Xiao liu Xiaoping Zhang longjie li Shaogang Wang Xianjin Xiao Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department and Institute of Urology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department and Institute of Urology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China School of life Science and Technology Wuhan Polytechnic UniversityWuhan 430030China Department of Laboratory Medicine Tongji hospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

DNA circuits are powerful tools in various applications such as logical computation,molecular diagnosis and synthetic *** is a major problem in constructing complex DNA *** directly affects the output signal and harms the circuit’s performance *** the traditional DNA circuits,the gate complex is a duplex *** are insufficient energy barriers to prevent spontaneous detachment of strands,resulting in a leak ***,we have developed triplex-structure based DNA circuit with ultra-low leakage and high signal-to-noise ratio(SNR).The triplex structure improves the stability in the absence of *** the same time,the driving force of the strand displacement cascades reduces the influence of the triplex structure on the desired *** SNR of the DNA circuit was increased to 695,while the desired reaction rate remained 90%of the conventional translator *** triplex-structure mediated leakage prevention strategy was further tested at different temperatures and in DNA translator and seesaw *** also constructed modular basic logic gates with a high efficiency and low *** this basis,we further constructed triplex-structure based tertiary DNA logic circuits,and the SNR reached 295,which,to the best of our knowledge,was among the highest of the *** believe that our scheme provides a novel,valid,and general tool for reducing leakages,and we anticipate that it will be widely adopted in DNA nanotechnology.

关键词： Leakage DNA strand displacement cascades Triplex DNA circuits Molecular programming

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于RGB图像处理预测哈密瓜叶片叶绿素研究

引用

中国农机化学报 2024年第6期45卷 149-155页

作者：李龙杰史勇刘彦岑郭俊先新疆农业大学机电工程学院乌鲁木齐市830052 新疆智能农业装备重点实验室乌鲁木齐市830052

为提高植物叶绿素检测设备的普遍性和实用性,通过研究手机和单片机拍摄的RGB图像与植物叶片叶绿素含量有无拟合关系,以图像处理的方式进行叶绿素预测的相关试验,为将来基于深度学习的植物叶绿素动态无损检测提供试验依据。通过OpenCV对... 详细信息

为提高植物叶绿素检测设备的普遍性和实用性,通过研究手机和单片机拍摄的RGB图像与植物叶片叶绿素含量有无拟合关系,以图像处理的方式进行叶绿素预测的相关试验,为将来基于深度学习的植物叶绿素动态无损检测提供试验依据。通过OpenCV对图像提取感兴趣区域(RoI),并进行均值滤波、高斯滤波和中值滤波,对原图和三种滤波后的图像进行三通道颜色特征分离,利用最小二乘法(LS)将颜色特征参数的多种组合与叶绿素实测值进行拟合分析,发现4种图像中均值滤波的拟合效果都普遍较好。在均值滤波中,手机K40拍摄的图像存在(B-G-R)/(B+G)特征组合与叶片叶绿素拟合决定系数为0.912。单片机ESP32_CAM拍摄的图像存在(G-B)B/(R+G)特征组合与叶片叶绿素拟合决定系数为0.778。运用梯度运算将均值滤波的RoI进行迭代处理,发现K40的决定系数略微下降,ESP32_CAM的决定系数出现好转。通过对K40与ESP32_CAM进行预测模型验证,两者都表现为随机森林(RF)回归模型的性能最好,在K40中训练集决定系数为0.953、训练集均方根误差为1.161,预测集决定系数为0.930、预测集均方根误差为1.516,在ESP32_CAM中训练集决定系数为0.794、训练集均方根误差为2.510,预测集决定系数为0.695、预测集均方根误差为2.985。

关键词：哈密瓜叶绿素 RGB图像图像识别回归预测

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Eff ects of environmental factors on the temporal and spatial variations in branch and leaf CO_(2)effl ux of Larix gmelinii ***-rupprechtii Mayr

引用

Journal of Forestry Research 2023年第4期34卷 1007-1019页

作者： longjie li Xiangzhen Wang Zhongkui Jia Key Laboratory for Silviculture and Conservation of the Ministry of Education College of ForestryBeijing Forestry UniversityBeijing 100083People’s Republic of China

The CO_(2)effl ux of branches and leaves plays an important role in ecosystem carbon *** a carbon fl ux system,the effl ux of Larix gmelinii ***(Dahurian larch)was investigated in 27 years(immature),31 years(near-mature),and 47 years(mature)stands at diurnal,seasonal,and spatial scales(direction and height)as well as its connection with environmental factors from May to October *** variation in effl ux was a single peak,and the maximum occurring between 14:00 and 16:*** variation also exhibited a single peak,with the maximum in late July and the minimum in early *** May to September,effl ux on the south side was the largest among the three stands,and mean values on the south side of 27 year-old,31 year-old,and 47 year-old trees were 0.50,0.97 and 1.05μmol·m^(–2)·s^(–1),*** minimum occurred on the north *** for the maximum in July and September in the 27 year-old stand in the middle of the canopy,the maximum effl ux in the upper canopy,and the means in the 27 year-old,31 year-old,and 47 year-old stands were 0.49,0.96 and 1.04μmol·m^(-2)·s^(-1),respectively;the minimum occurred in the lower *** and relative humidity infl uenced seasonal variations in effl *** variation in temperature sensitivity coeffi cient(Q 10)was opposite that of temperature,increasing with decreasing *** the spatial scale,maximum Q 10 occurred in the mid *** the effl ux and temperature data in diff erent locations,it is possible to better estimate effl ux variations in each stand.

关键词： CO_(2)effl ux Temperature sensitivity coeffi cient(Q 10) Temporal and spatial variations Environmental factors

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

高温高盐碎屑岩油藏注气用复合磺酸盐起泡剂性能

引用

石油学报（石油加工） 2024年第4期40卷 1117-1126页

作者：李隆杰葛际江刘加元李珂欣潘妍阎更平中国石油大学(华东)石油工程学院山东青岛266580 中国石油塔里木油田公司塔中采油气管理区新疆库尔勒841000 山东石油化工学院化学工程学院山东东营257061 中国石油塔里木油田公司勘探开发研究院新疆库尔勒841000

碎屑岩油藏岩石表面带负电,注气用起泡剂应优先选择阴离子表面活性剂。α-烯烃磺酸钠(AOS)是常用的阴离子类起泡剂,但在高盐含量水中溶解性差。将AOS和烷基二苯醚二磺酸钠(B2)复配,形成在矿化度1.12×10^(5) mg/L模拟地层水中具有较好... 详细信息

碎屑岩油藏岩石表面带负电,注气用起泡剂应优先选择阴离子表面活性剂。α-烯烃磺酸钠(AOS)是常用的阴离子类起泡剂,但在高盐含量水中溶解性差。将AOS和烷基二苯醚二磺酸钠(B2)复配,形成在矿化度1.12×10^(5) mg/L模拟地层水中具有较好溶解性的起泡剂AOS51(m(AOS)∶m(B2)=5∶1)。在温度110℃、压力30 MPa条件下,AOS51发泡率与在高盐条件下性能最好的羟磺基甜菜碱类起泡剂S23相当,而泡沫稳定性略逊于S23。静态吸附实验结果表明,AOS51在质量分数0.05%~1.0%范围内吸附量低于0.30 mg/g;起泡剂质量分数小于0.20%时AOS51与S23吸附量差异不大,但当起泡剂质量分数大于0.20%时S23的吸附量明显高于AOS51。岩心流动实验结果表明,质量分数0.10%和0.20%的AOS51在气测渗透率为80 mD和180 mD岩心中形成泡沫的阻力因子分别大于65、75和125、270,同时还表现出选择性流度降低特征;相同条件下AOS51流度调控能力优于S23。

关键词：碎屑岩高温高盐油藏注气起泡剂吸附量阻力因子 α-烯烃磺酸钠羟磺基甜菜碱

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

金属离子Mn^(2+)对α-烯烃磺酸钠/月桂酰胺丙基羟磺基甜菜碱蠕虫状胶束形成的调控作用研究

引用

日用化学工业（中英文） 2024年第6期54卷 621-629页

作者：储鹏举余晓玲侯克期王文慧李隆杰葛际江中国石油大学(华东)石油工程学院山东青岛266580 中国石化江苏油田分公司石油工程技术研究院江苏扬州225009

Mn^(2+)可诱导阴离子表面活性剂α-烯烃磺酸钠(AOS)与两性离子表面活性剂月桂酰胺丙基羟磺基甜菜碱(LHSB)的混合物形成蠕虫状胶束。在表面活性剂质量分数1%~5%范围内,研究了AOS与LHSB质量比、Mn^(2+)质量分数、表面活性剂质量分数及温... 详细信息

Mn^(2+)可诱导阴离子表面活性剂α-烯烃磺酸钠(AOS)与两性离子表面活性剂月桂酰胺丙基羟磺基甜菜碱(LHSB)的混合物形成蠕虫状胶束。在表面活性剂质量分数1%~5%范围内,研究了AOS与LHSB质量比、Mn^(2+)质量分数、表面活性剂质量分数及温度对蠕虫状胶束黏弹性的影响。结果表明,随着AOS与LHSB质量比从1:9变为9:1以及Mn^(2+)质量分数的增加,表面活性剂溶液黏度先增大后减小。体系的零剪切黏度随表面活性剂质量分数指数增加。常温下研究了表面活性剂黏度的变化规律,测得AOS与LHSB质量比为3:7时体系黏度最大,且当表面活性剂质量分数为2%时的最佳Mn^(2+)含量为0.5%,此时零剪切黏度为9030.9 mPa·s。用冷冻透射电镜(cryo-TEM)观察胶束形态,发现此时溶液中蠕虫状胶束较长,网络结构紧密,呈现出良好的线性黏弹性。表面活性剂质量分数、Mn^(2+)质量分数对蠕虫状胶束的形成有较大影响。

关键词：阴离子表面活性剂两性离子表面活性剂蠕虫状胶束金属离子调控

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Sensitive detection of alkaline phosphatase based on terminal deoxynucleotidyl transferase and endonuclease Ⅳ-assisted exponential signal amplification

引用

Journal of Pharmaceutical Analysis 2022年第4期12卷 692-697页

作者： Weicong Ye longjie li Zishan Feng Bocheng Tu Zhe Hu Xianjin Xiao Tongbo Wu Department of Pharmaceutical Analysis School of PharmacyTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Biopharmaceutics School of Life Science and TechnologyWuhan Polytechnic UniversityWuhan430023China Department of Translational Medicine Institute of Reproductive HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Alkaline phosphatase(ALP)is widely expressed in human *** plays an important role in the dephosphorylation of proteins and nucleic ***,quantitative analysis of ALP plays a vital role in disease diagnosis and the development of biological detection *** deoxynucleotidyl transferase(TdT)catalyzes continuous polymerization of deoxynucleotide triphosphates at the 30-OH end of single-stranded DNA in the absence of a *** this study,we developed a highly sensitive and selective method based on TdT and endonuclease Ⅳ(Endo Ⅳ)to quantify ALP *** ALP hydrolyzes the 30-PO_(4) end of the substrate and generates 30-OH,TdT can effectively elongate the 30-OH end with deoxynucleotide adenine triphosphate(dATP)and produce a poly A tail,which can be detected by the poly T *** Ⅳ digests the AP site in poly T probes to generate a fluorescent signal and a new 30-OH end,leading to the generation of exponential fluorescence signal *** substrate for TdT elongation was optimized,and a limit of detection of 4.3×10^(-3) U/L was achieved for ALP by the optimized substrate *** method can also detect ALP in the cell lysate of a single *** work has potential applications in disease diagnosis and biomedical detection.

关键词： Alkaline phosphatase Terminal deoxynucleotidyl transferase EndonucleaseⅣ Exponential amplification

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于数字图像的大田哈密瓜不同时期冠层相对叶绿素含量检测研究

引用

浙江农业学报 2024年第3期36卷 651-661页

作者：刘彦岑郭俊先郭阳史勇黄华李龙杰张振振新疆农业大学机电工程学院新疆乌鲁木齐830052 新疆农业大学数理学院新疆乌鲁木齐830052

为探究快速无损获取大田哈密瓜完整冠层相对叶绿素含量的可行性,首先,利用自制的图像采集小车搭载面阵相机,获取不同水肥处理下大田哈密瓜伸蔓期、开花期、膨果期的378张完整冠层图像;然后,对图像进行处理后,提取32种颜色特征和6种纹理... 详细信息

为探究快速无损获取大田哈密瓜完整冠层相对叶绿素含量的可行性,首先,利用自制的图像采集小车搭载面阵相机,获取不同水肥处理下大田哈密瓜伸蔓期、开花期、膨果期的378张完整冠层图像;然后,对图像进行处理后,提取32种颜色特征和6种纹理特征,分析图像特征与冠层相对叶绿素含量的相关性;接着,对图像特征进行预处理后,选取相应的主成分作为模型输入,分别建立用于预测不同时期冠层相对叶绿素含量的多元线性回归(MLR)、支持向量机回归(SVR)、随机森林(RF)模型。对比建模效果发现,SVR模型的效果最好,在伸蔓期、开花期、膨果期,该模型预测值与实测值回归的决定系数分别为0.73、0.73、0.83,均方根误差分别为0.90、0.91、0.76。研究表明,利用数字图像技术能实现对大田哈密瓜不同时期冠层相对叶绿素含量的快速无损检测,可为大田哈密瓜田间管理提供技术参考。

关键词：哈密瓜冠层图像叶绿素图像特征支持向量机回归关键生育期

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：