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Synergistic regulation of drought-responsive genes by transcription factor OsbZIP23 and histone modification in rice

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Journal of Integrative Plant Biology 2020年第6期62卷 723-729页

作者： Wei Zong Jun Yang Jie Fu lizhong xiong National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China

Thousands of differentially expressed genes(DEGs)have been identified in rice under drought stress ***,the regulatory mechanism of these DEGs remains largely ***,we report an interplay between histone H3K4me3 modification and transcription factor Osb ZIP23 in the regulation of a dehydrin gene cluster under drought stress conditions in *** the H3K4me3 modification level was increased,the dehydrin gene expression levels were increased,and the binding levels of Osb ZIP23 to the promoter of the dehydrin genes were also ***,the H3K4me3 modification and dehydrin gene expression levels were downregulated in the osbzip23 mutant under drought stress *** study uncovers a collaboration between transcription factor and H3K4me3 modification in the regulation of droughtresponsive genes,which will help us to further understand the gene regulation mechanism under stress conditions in plants.

关键词： responsive conditions. drought

Improving the resistance of the rice PTGMS line Feng39S by pyramiding blast,bacterial blight,and brown planthopper resistance genes

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The Crop Journal 2022年第4期10卷 1187-1197页

作者： Dabing Yang lizhong xiong Tongmin Mou Jiaming Mi National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070HubeiChina

Knowledge of rice(Oryza sativa L.)genes and various DNA markers can be used in genomic breeding programs aimed at developing improved elite rice *** used an efficient genomic breeding approach to pyramid four resistance genes(Pi2,Xa23,Bph14,and Bph15)in the popular photoperiod-and thermosensitive genic male sterile(PTGMS)rice line *** performed foreground selection for the target genes,followed by recombinant selection and background *** process reduced the sizes of the genomic segments harboring the target genes(566.8 kb for Pi2,1143.9 kb for Xa23,774.7 kb for Bph14,and 1574.9 kb for Bph15)and accelerated the recovery of the recurrent parent genome to proportions ranging from 98.77%to 99.16%,thus resulting in four near-isogenic *** assemble the four resistance genes in Feng39S,we performed a double-way cross combined with foreground and background selection to generate two improved lines of Feng39S(Pi2+Xa23+Bph14+Bph15)with a recurrent parent genome recovery of 98.98%.The two lines showed agronomic performance,grain quality,and fertility–sterility transition characteristics similar to those of the original Feng39S *** newly developed PTGMS lines and corresponding hybrid combinations were resistant to various field blast isolates and seven representative isolates of bacterial *** the seedling stage,the lines also showed resistance against brown *** study provides an efficient and accurate genomic breeding approach for introducing desirable traits into PTGMS lines.

关键词： Bacterial blight resistance Blast resistance Brown planthopper resistance Genomic breeding PTGMS line Two-line hybrid rice

GID1 modulates stomatal response and submergence tolerance involving abscisic acid and gibberellic acid signaling in rice

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Journal of Integrative Plant Biology 2015年第11期57卷 954-968页

作者： Hao Du Yu Chang Fei Huang lizhong xiong National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural University

Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Gibberellic acid （GA） and abscisic acid （ABA） play critical roles in the developmental programs and environmental responses, respectively, through complex signaling and metabolism networks. However, crosstalk between the two phytohormones in stress responses remains largely unknown. In this study, we report that GIBBERELLIN-INSENSITIVE DWARF 1 （GID1）, a soluble receptor for GA, regulates stomata[ develop- ment and patterning in rice （Oryza sativa L.）. The gidl mutant showed impaired biosynthesis of endogenous ABA under drought stress conditions, but it exhibited enhanced sensi- tivity to exogenous ABA. Scanning electron microscope and infrared thermal image analysis indicated an increase in the stomatal conductance in the gidl mutant under drought conditions. Interestingly, the gidl mutant had increased levels of chlorophyll and carbohydrates under submergence conditions, and showed enhanced reactive oxygen species （ROS）-scavenging ability and submergence tolerance compared with the wild-type. Further analyses suggested that the function of GID1 in submergence responses is partially dependent on ABA, and GA signaling by GID1 is involved in submergence tolerance by modulating carbohydrate consumption. Taken together, these findings suggest GID1 plays distinct roles in stomatal response and submergence tolerance through both the ABA and GA signaling pathways in rice.

关键词： Abscisic acid carbohydrateconsumption gibberellic acid stomata Oryza sativa

A structural view of the conserved domain of rice stress-responsive NAC1

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Protein & Cell 2011年第1期2卷 55-63页

作者： Qingfeng Chen Quan Wang lizhong xiong Zhiyong Lou National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070China Structural Biology Laboratory Tsinghua UniversityBeijing 100084China College of Life Sciences and Tianjin State Laboratory of Protein Science Nankai UniversityTianjin 300071China

The importance of NAC(named as NAM,ATAF1,2,and CUC2)proteins in plant development,transcription regulation and regulatory pathways involving proteinprotein interactions has been increasingly *** report here the high resolution crystal structure of SNAC1(stress-responsive NAC)NAC domain at 2.5Å.Although the structure of the SNAC1 NAC domain shares a structural similarity with the reported structure of the ANAC NAC1 domain,some key features,especially relating to two loop regions which potentially take the responsibility for DNA-binding,distinguish the SNAC1 NAC domain from other reported NAC ***,the dimerization of the SNAC1 NAC domain is demonstrated by both soluble and crystalline conditions,suggesting this dimeric state should be conserved in this type of NAC ***,we discuss the possible NAC-DNA binding model according to the structure and reported biological evidences.

关键词： stress-responsive NAC 1 NAC family DNA binding rice crystal structure

Determination of rice panicle numbers during heading by multi-angle imaging

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The Crop Journal 2015年第3期3卷 211-219页

作者： Lingfeng Duan Chenglong Huang Guoxing Chen lizhong xiong Qian Liu Wanneng Yang College of Engineering Huazhong Agricultural University Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River Huazhong Agricultural University National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research Huazhong Agricultural University

Plant phenomics has the potential to accelerate progress in understanding gene functions and environmental responses. Progress has been made in automating high-throughput plant phenotyping. However, few studies have investigated automated rice panicle counting. This paper describes a novel method for automatically and nonintrusively determining rice panicle numbers during the full heading stage by analyzing color images of rice plants taken from multiple angles. Pot-grown rice plants were transferred via an industrial conveyer to an imaging chamber. Color images from different angles were automatically acquired as a turntable rotated the plant. The images were then analyzed and the panicle number of each plant was determined. The image analysis pipeline consisted of extracting the i2 plane from the original color image, segmenting the image, discriminating the panicles from the rest of the plant using an artificial neural network, and calculating the panicle number in the current image. The panicle number of the plant was taken as the maximum of the panicle numbers extracted from all 12 multi-angle images. A total of 105 rice plants during the full heading stage were examined to test the performance of the method. The mean absolute error of the manual and automatic count was 0.5, with 95.3% of the plants yielding absolute errors within ± 1. The method will be useful for evaluating rice panicles and will serve as an important supplementary method for high-throughput rice phenotyping.

关键词： Plant phenotyping Rice panicle number Multi-angle imaging Image analysis

Integrative Regulation of Drought Escape through ABA-Dependent and -Independent Pathways in Rice

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Molecular Plant 2018年第4期11卷 584-597页

作者： Hao Du Fei Huang Nai Wu Xianghua Li Honghong Hu lizhong xiong National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070 China

Many plants have evolved a drought escape （DE） mechanism to shorten their life cycle when facing water- deficit conditions. While drought tolerance has been intensively investigated, the genetic and molecular mechanisms of DE remain elusive. In this study, we found that low water-deficit treatment （LWT） at the early stage of rice development can trigger early flowering and reduced tiller numbers. LWT induced the accu- mulation of abscisic acid （ABA）, which in turn has feed-back effects on light perception and circadian clock by synchronously regulating many flowering-related genes to promote early flowering. Moreover, some of light receptors, circadian components, and flowering-related genes including OsTOC1, Ghd7, and PhyB were found to be involved in LWT in an ABA-dependent manner, whereas some of the other flowering- related genes including OsGI, OsELF3, OsPRR37, and OsMADS50 were involved in the regulation of DE independent of ABA. In addition, we found that strigolactones and OsTB1 are involved in the tillering inhibition under LWT, which is independent of the flowering pathway in rice. Taken together, *** provide compelling evidence that DE in rice is coordinately regulated by multiple pathways during the reproduction （flowering） switch.

关键词： Oryza drought escape abscisic acid strigolactone circadian clock

Detecting quantitative trait loci for water use efficiency in rice using a recombinant inbred line population

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Chinese Science Bulletin 2011年第14期56卷 1481-1487页

作者： ZHOU GuangSheng LIU Fang CAO JinHua YUE Bing xiong lizhong College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070. China

Breeding rice with high water use efficiency (WUE) can ameliorate water shortage through water-saving ***,WUE is a complex quantitative trait and very few studies have been conducted to measure WUE *** this study,a recombined inbred line population derived from a cross between an indica lowland rice and upland japonica rice was used to dissect the genetic control of WUE by fine-monitored water supply *** trait loci (QTL) were scanned for 10 traits including heading date (HD),water-consumption per day (water/d),shoot weight gain per day (shootw/d),root weight gain per day (rootw/d),kernel weight gain per day (kernelw/d),average WUE at whole plant level (WUEwhole/d),average WUE for up-ground biomass (WUEup/d),average WUE for grain yield (WUEyield/d),average economic index (econindex/d),and average root/shoot ratio per day (ratio/d).The results show that most of the traits were significantly correlated to each ***-four QTL (LOD ≥ 2.0) were detected for econindex,econindex/d,WUEyield,WUEyield/d,WUEup,WUEup/d,WUEwhole,WUEwhole/d,kernelw,kernelw/d,rootw,and water/d by composite interval *** QTLs are located on chromosomes 1,2,4,6,7,8,and *** QTLs accounted for 4.97%-10.78% of the phenotypic variation *** of these QTLs overlapped with previously reported drought resistance QTLs detected in this *** results provide useful information for further dissection of the genetic basis and marker-assisted selection of WUE in rice.

关键词：水分利用效率数量性状位点重组自交系群体籼型水稻检测分子标记辅助选择水资源短缺问题复合区间作图法

Rice Gene Network Inferred from Expression Profiling of Plants Overexpressing OsWRKY13, a Positive Regulator of Disease Resistance

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Molecular Plant 2008年第3期1卷 538-551页

作者： Deyun Qiu Jun Xiao Weibo Xie Hongbo Liu Xianghua Li lizhong xiong Shiping Wang National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research (Wuhan) Huazhong Agricultural UniversityWuhan 430070 China

Accumulating information indicates that plant disease resistance signaling pathways frequently interact with other pathways regulating developmental processes or abiotic stress responses. However, the molecular mechanisms of these types of crosstalk remain poorly understood in most cases. Here we report that OsWRKY13, an activator of rice resistance to both bacterial and fungal pathogens, appears to function as a convergent point for crosstalk among the pathogen-induced salicylate-dependent defense pathway and five other physiologic pathways. Genome-wide analysis of the expression profiles of OsWRKY13-overexpressing lines suggests that OsWRKY13 directly or indirectly regulates the expression of more than 500 genes that are potentially involved in different physiologic processes according to the classification of the Gene Ontology database. By comparing the expression patterns of genes functioning in known pathways or cellular processes of pathogen infection and the phenotypes between OsWRKY13-overexpressing and wildtype plants, our data suggest that OsWRKY13 is also a regulator of other physiologic processes during pathogen infection. The OsWRKY13-associated disease resistance pathway synergistically interacts via OsWRKY13 with the glutathione/ glutaredoxin system and flavonoid biosynthesis pathway to monitor redox homeostasis and to putatively enhance the biosynthesis of antimicrobial flavonoid phytoalexins, respectively, in OsWRKY13-overexpressing lines. Meanwhile, the OsWRKY13-associated disease resistance pathway appears to interact antagonistically with the SNACl-mediated abiotic stress defense pathway, jasmonic acid signaling pathway, and terpenoid metabolism pathway via OsWRKY13 to suppress salt and cold defense responses as well as to putatively retard rice growth and development.

关键词： abiotic stress bacterial blight microarray Oryza sativa signal transduction.

Mutant Resources for the Functional Analysis of the Rice Genome

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Molecular Plant 2013年第3期6卷 596-604页

作者： Nili Wang Tuan Long Wen Yao lizhong xiong Qifa Zhang Changyin Wu National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research Huazhong Agricultural University Wuhan 430070 China

Rice is one of the most important crops worldwide, both as a staple food and as a model system for genomic research. In order to systematically assign functions to all predicted genes in the rice genome, a large number of rice mutant lines, including those created by T-DNA insertion, Ds/dSpm tagging, Tos17 tagging, and chemical/irradiation mutagenesis, have been generated by groups around the world. In this study, we have reviewed the current status of mutant resources for functional analysis of the rice genome. A total of 246 566 flanking sequence tags from rice mutant libraries with T-DNA, Ds/dSpm, or Tos17 insertion have been collected and analyzed. The results show that, among 211 470 unique hits, inserts located in the genic region account for 68.16%, and 60.49% of nuclear genes contain at least one insertion. Currently, 57% of non-transposable-element-related genes in rice have insertional tags. In addition, chemical/irradiation-induced rice mutant libraries have contributed a lot to both gene identification and new technology for the identification of mutant sites. In this review, we summarize how these tools have been used to generate a large collection of mutants. In addition, we discuss the merits of classic mutation strategies. In order to achieve saturation of mutagenesis in rice, DNA targeting, and new resources like RiceFox for gene functional identification are reviewed from a perspective of the future generation of rice mutant resources.

关键词： functional genomics mutagenesis mutants rice.