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(U-Th)/He热年代学方法研究进展及其应用

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矿物学报 2024年第2期44卷 273-286页

作者：胡儒权武丽艳中国科学院地球化学研究所矿床地球化学国家重点实验室贵州贵阳550081 中国科学院大学北京100049

(U-Th)/He热年代学方法基于矿物中U、Th、Sm发生衰变产生和累积的4He进行定年。由于低的封闭温度和对近地表地质过程的敏感性,因此在定量揭示地壳浅部所经历冷却及剥露事件的时间-空间-温度特征方面具有不可替代的优势。本文简要回顾了(... 详细信息

(U-Th)/He热年代学方法基于矿物中U、Th、Sm发生衰变产生和累积的4He进行定年。由于低的封闭温度和对近地表地质过程的敏感性,因此在定量揭示地壳浅部所经历冷却及剥露事件的时间-空间-温度特征方面具有不可替代的优势。本文简要回顾了(U-Th)/He定年原理并对不同测试方法的优缺点进行评述,在此基础上总结探讨了(U-Th)/He热年代学在辐射损伤影响机制、退火动力学以及4He扩散模型方面取得的进展以及存在的不足,并对如何完善这些不足进行未来展望。此外本文还综述了(U-Th)/He热年代学方法在约束成矿时代、造山带剥露历史研究、矿床保存性评价方面的应用实例以及部分局限性。

关键词： (U-Th)/He热年代学辐射损伤退火动力学 4He扩散模型地质应用

新叙事轻喜剧新解读——评新编川剧《梦回东坡》

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四川戏剧 2024年第1期 45-48页

作者：艾莲吴丽燕四川省社会科学院文学研究所四川省社会科学院

新编川剧《梦回东坡》以梦境为剧情,以东坡为核心,塑造出一个立体生动、别具一格的苏东坡形象。一是采用散文化的叙事技巧,形成了形散神不散的新叙事特点,将苏东坡的人生与感悟集中于黄州、惠州、儋州、常州四地,高度适应舞台时空;二是... 详细信息

新编川剧《梦回东坡》以梦境为剧情,以东坡为核心,塑造出一个立体生动、别具一格的苏东坡形象。一是采用散文化的叙事技巧,形成了形散神不散的新叙事特点,将苏东坡的人生与感悟集中于黄州、惠州、儋州、常州四地,高度适应舞台时空;二是巧用巴蜀方言,表现人物幽默风趣的品质,并别出心裁借人物之口戏仿苏东坡名篇做《赤壁赋》方言版,颇具轻喜剧特色;三是注重新解读,以和解重构剧中人与人、人与内心的关系,让观众深刻体悟苏东坡的达观心态。

关键词：《梦回东坡》新叙事轻喜剧新解读

切除联合自体角膜缘干细胞移植术治疗原发性翼状胬肉的研究

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浙江创伤外科 2024年第2期29卷 229-231页

作者：吴丽燕金燕娟浙江省杭州市西溪医院杭州310023 建德市中西医结合医院

目的探讨翼状胬肉切除联合自体角膜缘干细胞移植术治疗原发性翼状胬肉的临床效果及安全性。方法回顾性研究2019年1月至2023年1月在本院接受原发性翼状胬肉手术治疗的66例患者病历资料,将66例患者根据手术方式的不同分为对照组(n=33,翼... 详细信息

目的探讨翼状胬肉切除联合自体角膜缘干细胞移植术治疗原发性翼状胬肉的临床效果及安全性。方法回顾性研究2019年1月至2023年1月在本院接受原发性翼状胬肉手术治疗的66例患者病历资料,将66例患者根据手术方式的不同分为对照组(n=33,翼状胬肉切除联合游离结膜瓣移植术)及试验组(n=33,翼状胬肉切除联合自体角膜缘干细胞移植术)。比较两组临床效果、术后结膜瓣愈合时间、角膜上皮修复时间,记录术前及术后1个月、3个月、6个月泪膜破裂时间(TBUT)变化及术后并发症发生率和术后6个月的复发率。结果试验组患者治疗后的临床疗效高于对照组,术后结膜瓣愈合时间、角膜上皮修复时间明显短于对照组,术后的1月、3月、6月时TBUT时间明显长于对照组(P0.05)。结论翼状胬肉切除联合自体角膜缘干细胞移植术治疗原发性翼状胬肉具有良好的治疗效果和安全性,可降低术后复发率,且不增加术后并发症发生率。

关键词：原发性翼状胬肉翼状胬肉切除自体角膜缘干细胞移植术效果

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基于水作用下红黏土的承载力试验研究

土工基础 2024年第3期38卷 547-551页

作者：陈道松吴丽艳崔玮广西华安建设工程质量检测有限公司南宁530021 广西华蓝岩土工程有限公司南宁530001

以广西崇左地区红黏土为研究对象,开展水作用下承载力特性相关试验研究,结果表明:红黏土土体中水分蒸发迁移后,土体表面及土体产生裂隙,表面上的裂隙呈环状不规则形态,断面上的裂隙呈上宽下窄的形态。在遇水后,水沿裂隙渗入土体中,裂隙... 详细信息

以广西崇左地区红黏土为研究对象,开展水作用下承载力特性相关试验研究,结果表明:红黏土土体中水分蒸发迁移后,土体表面及土体产生裂隙,表面上的裂隙呈环状不规则形态,断面上的裂隙呈上宽下窄的形态。在遇水后,水沿裂隙渗入土体中,裂隙面土体崩解,裂隙中充满水,致使土体软化,承载力降低。对天然含水率状态及浸泡10 h状态下的红黏土,采用轻型动力触探和载荷试验研究其承载力的变化,发现以动力触探试验击数推定的承载力与载荷试验确定的承载力降低幅度基本一致。施工工程中如遇基槽遇水,可采用轻型动力触探试验判定其软化程度,对实际工程有很好参考价值。

关键词：红黏土承载力裂隙轻型动力触探载荷试验水作用

Determining the effects of LFHV-PEF treatment on the water distribution and vigor of aged rice seeds using LF-NMR

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International Journal of Agricultural and Biological Engineering 2022年第4期15卷 190-196页

作者： Ensi Cheng Ping Song Tiangang Hou liyan wu Benhua Zhang College of Engineering Shenyang Agricultural UniversityShenyang 110866China College of Information and Electrical Engineering Shenyang Agricultural UniversityShenyang 110866China School of Mechanical and Electrical Engineering Suqian UniversitySuqian 223800JiangsuChina

This study aimed to investigate the effect of low-frequency high-voltage pulsed electric field (LFHV-PEF) treatment on the germination of aged rice seeds. Aged rice seeds were subjected to LFHV-PEF treatment with different electric field strengths, and low-field nuclear magnetic resonance (LF-NMR) was performed to acquire the LF-NMR data of rice seeds at different germination periods during a standard seed germination test to analyze their internal patterns of water state and distribution. Optimal treatment conditions were determined based on the physicochemical data collected during germination, and the improvements in seed vigor were verified. The findings indicated that during germination, the contents of bound and semi-bound water within the aged rice seeds initially increased and then decreased, whereas free water and total water contents increased continuously and rapidly. Side peaks were also observed within the seeds. Under the LFHV-PEF treatment, the semi-bound water within the seeds was more easily converted to free water, and the water absorption rate, germination potential, germination rate, germination index, and vigor index of these seeds improved. Further, the optimal electrical field strength was 12 kV. By analyzing the internal patterns of water state and distribution in seeds, the mechanism by which electric field treatment improved seed vigor was elucidated, thus, providing theoretical support and data evidence for research on water absorption during the germination of rice seeds, and methods for improving seed vigor.

关键词： aged rice seeds germination water phase seed vigor LF-NMR LFHV-PEF

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分子植物育种 2024年第8期22卷 2464-2472页

作者：尹梦莹蔚亚楠龚亚菊桂敏鲍锐黎志彬吴丽艳杜光辉云南大学资源植物研究院昆明650500 云南省农业科学院园艺作物研究所昆明650205

黄萎病是茄子生产中的重要病害之一,严重影响茄子的产量和品质。WRKY转录因子在植物抗病中扮演着重要的角色,但在茄子黄萎病抗性中的作用不明。本研究从黄萎病高抗材料野生蒜芥茄的转录组数据中,筛选获得WRKY家族中的SsWRKY22和SsWRKY3... 详细信息

黄萎病是茄子生产中的重要病害之一,严重影响茄子的产量和品质。WRKY转录因子在植物抗病中扮演着重要的角色,但在茄子黄萎病抗性中的作用不明。本研究从黄萎病高抗材料野生蒜芥茄的转录组数据中,筛选获得WRKY家族中的SsWRKY22和SsWRKY33基因,利用PCR技术克隆获得目的基因,并对其进行生物信息学和表达模式分析。结果表明,SsWRKY22和SsWRKY33基因的ORF序列大小分别为528和1073 bp,编码175个和357个氨基酸;蛋白分子量分别为19.19和40.46 kD,均属于亲水性不稳定蛋白,其中,Ss WRKY22编码的蛋白属于酸性蛋白,而Ss WRKY33编码的蛋白属于碱性蛋白,亚细胞定位预测两个基因编码的蛋白均定位于细胞核上;此外,SsWRKY22和SsWRKY33分别属于Ⅱ类和Ⅰ类WRKY转录因子,具有不同的蛋白结构。系统发育分析结果表明,Ss WRKY22与野生潘那利番茄、番茄和马铃薯中的WRKY22亲缘关系较近;而Ss WRKY33与辣椒中的WRKY33亲缘关系最近。利用RT-qPCR对SsWRKY22和SsWRKY33基因在蒜芥茄不同部位(根,茎,叶)的表达模式进行分析,发现二者的表达具有组织特异性,且在根部的表达量均为最高。在接种黄萎病病菌后,SsWRKY22和SsWRKY33基因在蒜芥茄根部的表达量显著下调,表明SsWRKY22和SsWRKY33可能是蒜芥茄响应黄萎病胁迫的负调控因子。本研究首次克隆了云南野生蒜芥茄的WRKY转录因子中的SsWRKY22和SsWRKY33基因并解析其表达模式,为后续SsWRKY22和SsWRKY33的功能鉴定提供基础。

关键词：蒜芥茄黄萎病 SsWRKY22 SsWRKY33 表达模式

Transcriptome Analysis of a Wild Eggplant Germplasm M239 in Response to Verticillium dahliae Infection

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 591-609页

作者： liyan wu Jie Cheng Yaju Gong Rui Bao Zhibin Li Min Gui Guanghui Du Institute of Horticultural Crops Yunnan Academy of Agricultural SciencesKunming650205China Institute of Plant Resources Yunnan UniversityKunming650500China

In this study,wild eggplant germplasm No.M239,which is highly susceptible to Verticillium wilt,was used as the experimental *** physiological and biochemical indices(SOD,PAL,MDA and soluble protein)of M239 roots were measured at different times(0,12,24,36,48,60 and 72 h)post inoculation with Verticillium dahliae,and the key time points for the M239 response to Verticillium wilt infection were ***,RNA-Seq technology was used to screen the differentially expressed genes(DEGs)in M239 roots at 0,12 and 48 h post-inoculation(hpi).The transcriptional results of M239 were also compared with those resistance genes from some reported wild relative Solanum species(*** and ***).Then some DEGs were chosen for validation by qRT–*** results showed that 12 and 48 hpi were the turning points in the changes in all physiological and biochemical indices.A total of 6,783 DEGs were identified by RNA-Seq,including 6,141 DEGs(3,046 upregulated and 3,095 downregulated)at the M_12 h vs.M_0 h,1,903 DEGs(792 upregulated and 1,111 downregulated)at M_48 h vs.M_12 h,and 1,261 DEGs that appeared simultaneously in both *** enrichment analysis showed that there were 5 metabolic pathways enriched from DEGs,which were mostly related to primary metabolism,such as glycolysis,amino acid and ribosome *** with the NCBI non-redundant protein(NR)database,one Ve2 homologous gene and 8 PR protein-related genes were *** factor analysis showed that there were a large number of DEGs,such as MYB,AP2-EREBP,bHLH,NAC and Orphans in the two *** with the reported Verticillium wilt-resistant wild eggplant species,it was found that there were fewer genes and enriched metabolic pathways in the M239 response to Verticillium wilt infection,and it also lacked the response of some known key resistance *** results proved that the above resistance genes and metabolic pathways played a key role in the wild eggplant r

关键词： Wild eggplant Verticillium wilt transcriptome sequencing differentially expressed gene KEGG pathway

风电变桨轴承待机工况滚道磨损系数的确定方法

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机械设计与制造工程 2024年第8期53卷 113-117页

作者：张雨航丁世琛吴莉艳侯建昌吴凤和郭保苏燕山大学机械工程学院河北秦皇岛066004 信发集团有限公司山东聊城252100

为解决风电变桨轴承在机组待机时,受外界风载影响发生微动磨损形成伪布氏压痕的问题,以三排圆柱滚子轴承滚道为研究对象,采取仿真与试验相结合的方法,建立轴承滚子滚道的有限元模型。提出了基于Archard磨损模型与微动磨损试验结合的磨... 详细信息

为解决风电变桨轴承在机组待机时,受外界风载影响发生微动磨损形成伪布氏压痕的问题,以三排圆柱滚子轴承滚道为研究对象,采取仿真与试验相结合的方法,建立轴承滚子滚道的有限元模型。提出了基于Archard磨损模型与微动磨损试验结合的磨损系数确定方法,实现了对风电变桨轴承待机工况滚道磨损系数的修正,有助于延长变桨轴承的使用寿命。

关键词：微动磨损伪布氏压痕变桨轴承磨损系数

Physiochemical and biological characteristics of fouling on landfill leachate treatment systems surface

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Journal of Environmental Sciences 2024年第1期135卷 59-71页

作者： Zhourui Gong Shu Yang Rui Zhang Yangqing Wang Xiaoqing wu liyan Song School of resources and environmental engineering Anhui UniversityHefei 230601China Anhui Shengjin Lake Wetland Ecology National Long-term Scientific Research Base Dongzhi 247230China Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment Ministry of EducationChongqing UniversityChongqing 400045China Chongqing Institute of Green and Intelligent Technology Chinese Academy of SciencesChongqing 400714China Xing Lu Huan Jing Co.LTD. Luzhou 646000China

Fouling of landfill leachate,a biofilm formation process on the surface of the collection system,migration pipeline and treatment system causes low efficiency of leachate transportation and treatment and increases cost for maintenance of those *** addition,landfill leachate fouling might accumulate pathogens and antibiotic resistance genes(ARGs),posing threats to the *** of the landfill leachate fouling and its associated environmental behavior is essential for the management of *** this study,physicochemical and biological properties of landfill leachate fouling and the possible accumulation capacity of pathogens and ARGs were investigated in nitrification(aerobic condition)and denitrification(anaerobic condition)process during landfill leachate biological treatment,*** show that microbial(bacterial,archaeal,eukaryotic,and viral)community structure and function(carbon fixation,methanogenesis,nitrification and denitrification)differed in fouling under aerobic and anaerobic conditions,driven by the supplemental leachate water *** fouling had a higher abundance of nitrification and denitrification functional genes,while anaerobic fouling harbored a higher abundance of carbon fixation and methanogenesis *** forms of leachate fouling had a higher abundance of pathogens and ARGs than the associated leachate,suggesting the accumulation capacity of fouling on biotic ***,aerobic fouling harbored three orders of magnitude higher multidrug resistance genes mex D than its associated *** finding provides fundamental knowledge on the biological properties of leachate fouling and suggests that leachate fouling might harbor significant pathogens and ARGs.

关键词： Landfill leachate fouling Biofilm Microbiome Carbon and nitrogen cycle Pathogens Antibiotic resistance genes