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中国实验方剂学杂志 2024年第19期30卷 116-123页

作者：常利娅冷玉芳赵紫岑王玉邢阳李东斌兰州大学第一临床医学院兰州730000 兰州大学第一医院兰州730000

目的:采用网络药理学和体内实验结合的方法研究黄芪甲苷(AS-Ⅳ)调控细胞焦亡(Pyroptosis)减轻肠缺血再灌注损伤(IRI)的作用机制。方法:首先从中药系统药理数据库和分析平台(TCMSP)数据库和Swiss Target Prediction数据库中检索获得AS-... 详细信息

目的:采用网络药理学和体内实验结合的方法研究黄芪甲苷(AS-Ⅳ)调控细胞焦亡(Pyroptosis)减轻肠缺血再灌注损伤(IRI)的作用机制。方法:首先从中药系统药理数据库和分析平台(TCMSP)数据库和Swiss Target Prediction数据库中检索获得AS-Ⅳ的对应靶点基因,并从GeneCards数据库中检索得到肠IRI和Pyroptosis相关的靶点基因,通过解螺旋网站绘制Venn图,获得三者的共同靶点基因。通过STRING数据库、Cytoscape软件构建蛋白-蛋白相互作用(PPI)网络筛选共同靶基因并导入Cytoscape软件获得核心靶基因。微生信平台用于基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析并预测AS-Ⅳ调控Pyroptosis减轻肠IRI的作用机制。然后采用随机数字表法,将C57/BL6J小鼠随机分为5组,正常组、模型组、给药组(IR+AS-Ⅳ)、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)激动剂NSS组(IR+AS-Ⅳ+NSS)、NLRP3抑制剂MCC950组(IR+AS-Ⅳ+MCC950)。模型组、给药组、NLRP3激动剂NSS组、NLRP3抑制剂MCC950组采用夹闭肠系膜上动脉45 min恢复灌注2 h建立肠IRI模型,正常组仅分离血管不夹闭。给药组、NLRP3激动剂NSS组、NLRP3抑制剂MCC950组于造模前连续3 d灌胃溶于0.1%二甲基亚砜的黄芪甲苷(50 mg·kg^(-1)),末次灌胃时间为造模前2 h,其余2组灌胃等量的生理盐水。NLRP3激动剂NSS组于造模前1 h腹腔注射4 mg·kg^(-1)的NSS,NLRP3抑制剂MCC950组于造模前1 h腹腔注射10 mg·kg^(-1)的MCC950。再灌注2 h处死小鼠,获取肠组织。采用酶联免疫吸附测定法(ELISA)检测白细胞介素(IL)-18,IL-1β水平,蛋白免疫印迹法(Western blot)检测硫氧还蛋白互作蛋白(TXNIP)、NLRP3、胱天蛋白酶(Caspase)-1、焦孔素D(GSDMD)蛋白表达,Chiu's评分评估小鼠肠组织病理学变化。结果:网络药理学分析表明肠IRI的靶点有1599个,AS-Ⅳ的作用靶点有199个,Pyroptosis的靶点有197个,三者共同靶点有20个。核心靶点有10个,包括NLRP3、TXNIP、沉默信息调节因子1(SIRT1)、高迁移率族蛋白1(HMGB1)、IL-18、GSDMD、基质金属蛋白酶-9(MMP-9)等。共同靶点的KEGG富集筛选出的通路相关性最大的是NOD样受体通路。体内实验结果表明,与正常组比较,模型组Chiu's评分升高,小鼠肠组织IL-18,IL-1β炎症因子水平明显升高(P<0.05),TXNIP、NLRP3、Caspase-1、GSDMD蛋白表达水平明显升高(P<0.05);与模型组比较,给药组和NLRP3抑制剂MCC950组Chiu's评分降低,小鼠肠组织IL-18,IL-1β炎症因子水平明显降低(P<0.05),TXNIP、NLRP3、Caspase-1、GSDMD蛋白表达水平明显降低(P<0.05);与给药组比较,NLRP3激动剂NSS组Chiu's评分升高,小鼠肠组织IL-18,IL-1β炎症因子水平明显升高(P<0.05),NLRP3、Caspase-1、GSDMD蛋白表达水平明显升高(P<0.05);与NLRP3抑制剂MCC950组比较,NLRP3激动剂NSS组Chiu's评分升高,小鼠肠组织IL-18,IL-1β炎症因子水平明显升高(P<0.05),TXNIP、NLRP3、Caspase-1、GSDMD蛋白表达水平明显升高(P<0.05)。结论:网络药理学预测结果与体内实验结果一致,黄芪甲苷通过TXNIP/NLRP3信号通路抑制细胞焦亡以减轻IRI。

关键词：黄芪甲苷肠缺血再灌注损伤硫氧还蛋白结合蛋白(TXNIP)/核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)信号通路细胞焦亡网络药理学

Ecological footprint analysis based on RS and GIS in arid land

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Journal of Geographical Sciences 2005年第1期15卷 44-52页

作者： changBin XIONGliya InstituteofGeographicSciencesandNaturalResourcesResearch CASBeijing100101China

Sustainable development has become a primary objective for many countries and regions throughout the world now. The ecological footprint (EF) is a kind of concise method of quantifiably measuring the natural capital consumption and it can reflect the goal of sustainability. In this paper, the concept, the theory and method of ecological footprint are introduced. On this basis, the study brings forward the method of ecological footprint and capacity prediction. The method is employed for the ecological footprint prediction combining consumption model with population model and the technique is adopted for the ecological capacity (EC) prediction uniting the Geographical Cellular Automata (Geo CA) and Geographic Information System (GIS). The above models and methods are employed to calculate EF and EC in 1995 and 2000 and predict them in 2005 in Hexi Corridor. The result shows that EF is continually increasing, and EC ascended in the anterior 5 years and will descend in the posterior 5 years. This suit of method is of the character of accuracy and speediness.

关键词： RS GIS Cellular Automata ecological footprint arid land Hexi Corridor

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行业特色型高校自动化一流本科专业建设

电气电子教学学报 2024年第2期46卷 52-55页

作者：杨盼盼闫茂德朱礼亚长安大学电子与控制工程学院西安710064

围绕长安大学办学定位、专业特色和学科优势,以自动化专业为改革与实践对象,构建“培养目标、师资队伍、课程体系、教学资源和保障机制”五位一体的专业建设模式,形成“汽车+交通+IT技术”的专业特色,初步建成契合社会经济发展需求、行... 详细信息

围绕长安大学办学定位、专业特色和学科优势,以自动化专业为改革与实践对象,构建“培养目标、师资队伍、课程体系、教学资源和保障机制”五位一体的专业建设模式,形成“汽车+交通+IT技术”的专业特色,初步建成契合社会经济发展需求、行业特色鲜明、广受社会认可的自动化一流本科专业。该模式对于促进自动化专业的建设与发展,培养具有社会责任感与创新精神的一流人才具有重要意义。

关键词：行业特色型高校自动化专业一流专业建设

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药物减轻心肌缺血再灌注损伤机制研究的进展

兰州大学学报（医学版） 2024年第4期50卷 88-94页

作者：邢阳冷玉芳赵潇李东阳兰金辉刘振李东斌王玉常利娅赵紫岑兰州大学第一临床医学院甘肃兰州730000 兰州大学第一医院麻醉手术科甘肃兰州730000 西安交通大学第二附属医院消化内科陕西西安710004 吉林大学白求恩第三临床医学院吉林长春130000

心肌缺血再灌注损伤常见于心肌梗塞、心肌缺血等,其发病率、死亡率高,且缺乏防治措施。积极探究防治心肌缺血再灌注损伤是临床研究的热点,近5年有关药物减轻心肌缺血再灌注损伤及其机制的研究较多,也取得了一些进展。本文着重介绍近5年... 详细信息

心肌缺血再灌注损伤常见于心肌梗塞、心肌缺血等,其发病率、死亡率高,且缺乏防治措施。积极探究防治心肌缺血再灌注损伤是临床研究的热点,近5年有关药物减轻心肌缺血再灌注损伤及其机制的研究较多,也取得了一些进展。本文着重介绍近5年关于药物通过抑制铁死亡、细胞凋亡、坏死性凋亡、细胞焦亡、氧化应激、钙超载、自噬调控、内质网应激、能量代谢等机制减轻心肌缺血再灌注损伤的实验研究和临床观察,及利用纳米技术、冠状动脉内给药技术等防治心肌缺血再灌注损伤的新成果,为心肌缺血再灌注损伤防治和研究提供理论依据。

关键词：药物心肌缺血再灌注器官保护细胞凋亡自噬氧化应激

Autologous hematopoietic stem cell transplantation and conventional insulin therapy in the treatment of children with newly diagnosed type I diabetes： long term follow-up

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Chinese Medical Journal 2014年第14期127卷 2618-2622页

作者： Gu Yi Gong Chunxiu Peng Xiaoxia Wei liya Su chang Qin Miao wang Xi'ou China 100045 China 100045

Background It has been indicated that autologous hematopoietic stem cell transplantation （AHST） is a promising treatment to adults with type 1 diabetes, however, the application of AHST therapy to children with type 1 diabetes still needs more data. The aim of this study was to assess the clinical effect of immune intervention combined with AHST and conventional insulin therapy in the treatment of children with newly diagnosed type 1 diabetes. Methods This 1：2 matched case-control study was comprised of 42 children who were newly diagnosed with type 1 diabetes in the Department of Endocrinology, Beijing Children＇s Hospital from 2009-2010. The case group included 14 patients, who were treated with AHST within the first 3 months after being diagnosed with diabetes at request of their parents during 2009-2010. The control group included 28 patients with newly diagnosed type 1 diabetes at the same period of hospitalization. We compared the baseline and follow-up data of them, including ketoacidosis onset, clinical variables （glycosylated hemoglobin （HbAlc）, insulin dosage and serum C-peptide）. Results The clinical characteristics of the patients was comparable between the case group and the control group. At 6-12 months （（10.7±4.2） months） after AHST treatment, we found 11 patients in the case group did not stop the insulin therapy, three cases stopped insulin treatment for 2, 3 and 11 months, respectively. No diabetic ketoacidosis （DKA） occurred after transplantation in all the patients in the case group. HbAlc in the control group was significant lower than that in the case group （P 〈0.01）, while the insulin dosage and serum C-peptide were not significant different between the two groups （P 〉0.05）. In order to eliminate the honeymoon effect, we performed final follow-up at the 3-5 years （（4.2±1.8） years） after AHST treatment, and found that HbAlc in the control group was still lower than that in the case group （P 〈0.01）; however, the insulin dosage and serum C-peptide

关键词： autologous hematopoietic stem cell transplantation insulin therapy children type 1 diabetes

发展资源在减少青少年外化问题行为中的累积效应及关系模式

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心理学报 2019年第11期51卷 1244-1255页

作者：常淑敏张丽娅王玲晓山东师范大学心理学院

以1219名初一学生为被试进行间隔一年的追踪研究,考察了发展资源对青少年当前和一年后外化问题行为的累积效应及其关系模式,并进一步探讨了性别在关系模式中可能存在的调节作用。结果表明:(1)发展资源对青少年当前和一年后外化问题行为... 详细信息

以1219名初一学生为被试进行间隔一年的追踪研究,考察了发展资源对青少年当前和一年后外化问题行为的累积效应及其关系模式,并进一步探讨了性别在关系模式中可能存在的调节作用。结果表明:(1)发展资源对青少年当前和一年后外化问题行为的减少均具有累积效应,具体而言,发展资源对青少年当前外化问题行为的累积效应呈二次函数的非线性模式,而对青少年一年后外化问题行为的累积效应呈线性模式;(2)性别的调节作用仅在累积发展资源与青少年当前外化问题行为间的二次函数模式中显著。研究结果为有针对性地进行资源构建,预防和减少青少年外化问题行为提供了依据。

关键词：发展资源外化问题行为累积效应关系模式调节作用

Development and Utilization of Water Resources and Countermeasures:A Case Study of Yuan'an County of Yichang City in Hubei Province

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Journal of Landscape Research 2016年第5期8卷 33-35,38页

作者： CHENG chang HU Yong ZHAO liya JIANG Liping GAO Dandan Faculty of Resources and Environmental Science Hubei University Plant Protection College Yunnan Agricultural University

[Objective] To study current development and utilization of water resources in Yuan'an County and countermeasures. [Method] On the basis of analyzing basic characteristics of water resources in Yuan'an County as well as its current development and utilization, problems in the development and utilization of water resources in the study area were pointed out, and feasible countermeasures were proposed correspondingly. [Result] The county is endowed with rich water resources, but the overall use efficiency is low. There are many problems in the development and utilization of local water resources, such as pollution of water source, water and soil loss and so on. [Conclusion] In view of the problems, feasible suggestions were given to provide scientific support for the reasonable development and utilization of water resources in Yuan'an County.

关键词： Water resources Yuan’an County Development and utilization Countermeasure

The Role of Nitric Oxide in Hyperoxic Lung Injury in Premature Rats

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2001年第1期21卷 78-81页

作者：常立文马丽亚张晓慧陈晔 Department of Pediatrics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

To investigate the role of nitric oxide (NO) in hyperoxic lung injury, the 3 day old preterm rats were randomly assigned to four groups: group I (hyperoxia group), group Ⅱ (hyperoxia+N w nitro L arginine methyl ester (L NAME) group), group Ⅲ (air group), and group Ⅳ (air+L NAME) group. Group Ⅰ and Ⅱ were exposed to ≥90 % O 2 for 3 or 7 days. Group Ⅱ and Ⅳ received subcutaneous L NAMEy on daily basis (20 mg/kg). After 3 day or 7 day exposure, the lung wet weight/dry weight ratio (W/D), total protein and malondialdehyde (MDA) in bronchoalveolar lavage fluid (BALF) and lung pathology were examined in all groups. NO content, expression of endothelial NOS (eNOS) and inducible NOS (iNOS) in lungs were measured in group Ⅰ and Ⅲ. Our results showed that after 3 day exposure, group Ⅰ appeared acute lung injury characterized by the increase of MDA content ( P changes were more severe than those after 3 day exposure. After 3 and 7 day exposure, total protein in group Ⅱ was significantly increased as compared with group Ⅰ ( P changes were more obvious in group Ⅱ than in group Ⅰ. Occasionally, mild hemorrhage was detected in the lungs of group Ⅳ. BALF protein content in group IV was higher than that in group Ⅲ after 7 day exposure ( P <0.01). After 3 and 7 day exposure, NO content in BALF were all significantly elevated in group Ⅰ as compared with group Ⅲ ( P <0.01 for all). In the lungs of group Ⅰ, strong immunostaining for iNOS was observed in airway and alveolar epithelia, inflammatory cells, which were stronger than those in group Ⅲ. Expression of iNOS in rats after 7 day hyperoxic exposure was stronger than that after 3 day exposure. Shortly after 7 day exposure, strong

关键词： nitric oxide hyperoxia lung premature infant rat

中考生家庭社会经济地位与学习投入的关系:父母教育期望和父母教养行为的多重中介作用

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中国特殊教育 2018年第12期 75-81页

作者：王玲晓张丽娅常淑敏山东师范大学心理学院济南250014

本研究以490名九年级学生为被试,采用家庭社会经济地位量表、学习投入量表、父母教育期望量表和简式父母教养方式问卷对其进行测查,综合考察家庭社会经济地位与学习投入之间的关系,以及父母教育期望和父母教养行为在其中的中介作用。结... 详细信息

本研究以490名九年级学生为被试,采用家庭社会经济地位量表、学习投入量表、父母教育期望量表和简式父母教养方式问卷对其进行测查,综合考察家庭社会经济地位与学习投入之间的关系,以及父母教育期望和父母教养行为在其中的中介作用。结果发现:(1)家庭社会经济地位、父母教育期望、父母积极教养行为、父母消极教养行为和学习投入间相关均显著。(2)家庭社会经济地位影响学习投入的路径有四条:家庭社会经济地位可以通过父母教育期望间接影响个体的学习投入;家庭社会经济地位可以通过父母积极教养行为间接影响个体的学习投入;家庭社会经济地位可以通过父母教育期望和父母积极教养行为间接影响个体的学习投入;家庭社会经济地位可以通过父母教育期望和父母消极教养行为间接影响个体的学习投入。(3)父母教育期望和父母教养行为在家庭社会经济地位和学习投入间起链式中介作用。

关键词：家庭社会经济地位父母教育期望父母积极教养行为父母消极教养行为学习投入