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WheatGmap:a comprehensive platform for wheat gene mapping and genomic studies

Molecular Plant 2021年第2期14卷 187-190页

作者： Lichao Zhang Chunhao Dong Zhongxu Chen lixuan gui Cheng Chen Danping Li Zhencheng Xie Qiang Zhang Xueying Zhang Chuan Xia Xu Liu Xiuying Kong Jirui Wang Key Laboratory for Crop Gene Resources and Germplasm Enhancement Ministry of Agriculture and Rural Affairs/National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130China Department of Life Science TcuniInc.Chengdu 610041China

Bulked segregant analysis(BSA)is an efficient and low-cost strategy that is widely used to identify causal genes in segregating ***-based methods,such as BSA sequencing(Wenger et al.,2010),bulked segregant RNA sequencing(BSR-seq)(del Viso et al.,2012),and MutMap(Abe et al.,2012),are powerful tools that can be used for rapidly discovering genetic markers and gene *** BSA is increasingly being used in wheat(Triticum aestivum)gene mapping efforts,few user-friendly BSA tools have been developed for researchers lacking a strong bioinformatics ***,we developed the web-based BSA platform WheatGmap(https://***),which integrates multiple BSA mapping models and large amounts of public data to accelerate gene cloning and functional research and facilitate resource sharing.

关键词： aestivum Wheat comprehensive

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Systematic identification of wheat spike developmental regulators by integrated multiomics, transcriptional network, GWAS, and genetic analyses

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Molecular Plant 2024年第3期17卷 438-459页

作者： Xuelei Lin Yongxin Xu Dongzhi Wang Yiman Yang Xiaoyu Zhang Xiaomin Bie lixuan gui Zhongxu Chen Yiliang Ding Long Mao Xueyong Zhang Fei Lu Xiansheng Zhang Cristobal Uauyo Xiangdong Fu Jun Xiao Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Nanjing Agricultural University NanjingJiangsu 210095China Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTaianShandong 271018China Department of Life Science Tcuni Inc.ChengduSichuan 610000China John Innes Centre Norwich Research ParkNorwich NR47UHUK Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China CAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS) Institute of Genetics and Developmental BiologyCASBeijing 100101China

The spike architecture of wheat plays a crucial role in determining grain number,making it a key trait for optimization in wheat breeding *** this study,we used a multi-omic approach to analyze the transcriptome and epigenome profiles of the young spike at eight developmental stages,revealing co-ordinated changes in chromatin accessibility and H3K27me3 abundance during the flowering *** constructed a core transcriptional regulatory network(TRN)that drives wheat spike formation and experimentally validated a multi-layer regulatorymodule involving TaSPL15,TaAGLG1,and *** integrating the TRN with genome-wide association studies,we identified 227 transcription factors,including 42 with known functions and 185 with unknown *** investigation of 61 novel transcription factors using multiple homozygous mutant lines revealed 36 transcription factors that regulate spike architecture or flowering time,such as TaMYC2-A1,TaMYB30-A1,and *** particular interest,TaMYB30-A1,downstream of and repressed by WFzP,was found to regulate fertile spikelet ***,the excellent haplotype of TaMYB30-A1,which contains a C allele at the WFzP binding site,was enriched during wheat breeding improvement in China,leading to improved agronomic ***,we constructed a free and open access Wheat Spike Multi-Omic Database(http://39.***.48.156:8800/#/).Our study identifies novel and high-confidence regulators and offers an effective strategy for dissecting the genetic basis of wheat spike development,with practical value forwheat breeding.

关键词： wheat spike development epigenome TRN TaMYB30-A1 breeding selection

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Boosting wheat functional genomics via an indexed EMS mutant library of KN9204

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Plant Communications 2023年第4期4卷 58-76页

作者： Dongzhi Wang Yongpeng Li Haojie Wang Yongxin Xu Yiman Yang Yuxin Zhou Zhongxu Chen Yuqing Zhou lixuan gui Yi Guo Chunjiang Zhou Wenqiang Tang Shuzhi Zheng Lei Wang Xiulin Guo Yingjun Zhang Fa Cui Xuelei Lin Yuling Jiao Yuehui He Junming Li Fei He Xigang Liu Jun Xiao Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Ministry of Education Key Laboratory of Molecular and Cellular Biology Hebei Key Laboratory of Molecular and Cellular BiologyCollege of Life SciencesHebei Normal UniversityHebei Collaboration Innovation Center for Cell SignalingShijiazhuang 050024China Center for Agricultural Resources Research Institute of Genetics and Development BiologyChinese Academy of SciencesShijiazhuang 050022China University of Chinese Academy of Sciences Beijing 100049China Department of Life Science TcuniIncChengdu 610000China State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life SciencesSchool of Life SciencesCenter for Quantitative BiologyPeking UniversityBeijing 100871China State Key Laboratory of Protein and Plant Gene Research School of Advanced Agricultural SciencesPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China Plant Genetic Engineering Center of Hebei Province Institute of Biotechnology and Food ScienceHebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051China Institute ofCereal and OilCrops Hebei Academy of Agriculture and Forestry SciencesLaboratory of CropGenetics andBreeding ofHebeiShijiazhuang 050035China Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong College of AgricultureLudong UniversityYantai 264025China Centre of Excellence for Plant and Microbial Science(CEPAMS) JIC-CASBeijing 100101China

A better understanding of wheat functional genomics can improve targeted breeding for better agronomic traits and environmental ***,the lack of gene-indexed mutants and the low transformation efficiency of wheat limit in-depth gene functional studies and genetic manipulation for *** this study,we created a library for KN9204,a popular wheat variety in northern China,with a reference genome,transcriptome,and epigenome of different tissues,using ethyl methyl sulfonate(EMS)*** library contains a vast developmental diversity of critical tissues and transition *** capture sequencing of 2090 mutant lines using KN9204 genome-designed probes revealed that 98.79%of coding genes had mutations,and each line had an average of 1383 EMS-type *** identified new allelic variations for crucial agronomic trait-related genes such as Rht-D1,Q,TaTB1,and *** tested 100 lines with severemutations in 80 NAC transcription factors(TFs)under drought and salinity stress and identified 13 lines with altered *** analysis of three lines using transcriptome and chromatin accessibility data revealed hundreds of direct NAC targets with altered transcription patterns under salt or drought stress,including SNAC1,DREB2B,CML16,and ZFP182,factors known to respond to abiotic ***,we have generated and indexed a KN9204 EMS mutant library that can facilitate functional genomics research and offer resources for genetic manipulation of wheat.

关键词： wheat exome capture sequencing EMS mutagenesis functional genomics

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归桃麻子仁丸加减治疗老年2型糖尿病便秘的临床观察

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中医临床研究 2021年第18期13卷 65-67,75页

作者：邢利旋黄少妮林华容林丽莉潮州市中心医院广东潮州521000

目的:观察经典方剂麻子仁丸加用化瘀通络药物当归、桃仁在老年糖尿病便秘患者中的疗效。方法:采用病例随机对照研究方法,随机选取2019-2020年潮州市中心医院老年2型糖尿病患者60例做为研究对象,分为治疗组和对照组各30例。通过观察指标... 详细信息

目的:观察经典方剂麻子仁丸加用化瘀通络药物当归、桃仁在老年糖尿病便秘患者中的疗效。方法:采用病例随机对照研究方法,随机选取2019-2020年潮州市中心医院老年2型糖尿病患者60例做为研究对象,分为治疗组和对照组各30例。通过观察指标糖化血红蛋白(Glycosylated Hemoglobin,HbA1C)变化、便秘症状评分前后变化进行疗效判定。结果:中药治疗组的临床总有效率和症状总积分的改善比西药对照组更具优势,两组之间的差异具有统计学意义;并且对排便周期、大便性状改善情况明显优于对照组。结论:治病必求本是中医学的治疗原则也是优势,同样在糖尿病便秘的治疗中,应从病因病机出发,审证求因,标本同治。血瘀机制在糖尿病便秘发病过程中的影响不容忽视,在润肠通便的基础上加用化瘀通络是可行的,为糖尿病便秘的中医临床治疗提供更加广阔的思路。

关键词：老年糖尿病便秘中医疗法归桃麻子仁丸加减

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