检索结果-南通市图书馆

Rickettsia-like organism infection associated with mass mortalities of blood clam, Tegillarca granosa, in the Yueqing Bay in China

引用

Acta Oceanologica Sinica 2012年第1期31卷 106-115页

作者： ZHU Zewen XU Ting HE Zhongyang wu Xinzhong wu liuji MENG Qingguo Laboratory of Marine Life Science and Technology College of Animal SciencesZhejiang UniversityHangzhou 310029China Fisheries Technical Extension Center of Zhejiang Province Hangzhou 310012China

A series of mass mortalities of the cultured blood clam, Tegillarca granosa, occurred in the Yueqing Bay of China from 2005 to 2009. An obligate intracellular prokaryote, designated as rickettsia-like organism （RLO）, was frequently found in the moribund or dead blood clam sample during ultra- structural examination. These organisms were usually round, ellipsoid or occasionally dumbbell- shaped, ranged from approximately 0.28 to 0.71 #m in size and had a trilaminar cell wall. Two reproductive modes of organisms, transverse binary fission and budding, were observed. The or- ganisms were able to form eosinophilic inclusions. Most inclusions were found within epithelial and connective tissues of the mantle, gills and digestive tube. The biological and morphological char- acteristics indicate that these organisms may belong to the family Rickettsiaceae. RLOs exhibited significant pathogenicity. Cytopathological examinations revealed extensive necrosis and destruc- tion in the infected cell. The degree of tissue destruction was positively related to the number of RLO inclusions in the tissues, and the cytopathological effects were positively related to the number of intracellular RLO. RLOs and their inclusions were discovered throughout different disease areas and in different time periods. The infection intensity of the RLOs was positively correlated with the mortality rate of clams. Therefore, RLO infection might be associated with mass mortalities of cultured blood clams in the Yueqing Bay.

关键词： blood clam Tegillarca granosa rickettsia-like organism (RLO) inclusions mass mortalities

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular and functional characterization of ferritin in abalone Haliotis diversicolor supertexta

引用

Acta Oceanologica Sinica 2012年第3期31卷 87-97页

作者： XIE Jiasong CAO Xiaohua wu liuji LUO Ming ZHU Zewen HUANG Yanqing wu Xinzhong Laboratory of Marine Life Science and Technology College of Animal SciencesZhejiang UniversityHangzhou 310058China

Ferritin is an iron storage protein that plays a key role in the processes of physiology and *** the present study,the authors reported the ferritin gene from abalone Haliotis diversicolor supertexta,which we named *** full-length of hds-ferritin cDNA consisted of 879 bp with an ORF encoding a 171 amino *** acid sequence analysis revealed that hds-ferritin shared highly homology with other *** time PCR and western blot analysis showed that hds-ferritin was distributed ubiquitously in abalone tissues and had the highest expression level in digestive glands,but its transcripts are not modified remarkably by the stimulation with *** recombinant protein was successfully expressed in Escherichia coli BL21 (DE3),and the titre of anti-ferritin antibody was about 1∶*** effects of ROS and RNS on ferritin were analyzed in the present *** results showed that H2O2 played an important role in decreasing hds-ferritin,however NO cation appeared to have a protecting effect on H2O2-medied reduction of hds-ferritin.

关键词： Haliotis diversicolor supertexta ferritin respiratory burst oxidative damage immune response

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Large-Scale Discovery of Non-conventional Peptides in Maize and Arabidopsis through an Integrated Peptidogenomic Pipeline

引用

Molecular Plant 2020年第7期13卷 1078-1093页

作者： Shunxi Wang Lei Tian Haijun Liu Xiang Li Jinghua Zhang Xueyan Chen Xingmeng Jia Xu Zheng Shubiao wu Yanhui Chen Jianbing Yan liuji wu National Key Laboratory of Wheat and Maize Crop Science Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China School of Environmental and Rural Science University of New EnglandArmidaleNSW 2351Australia

Non-conventional peptides(NCPs),which include small open reading frame-encoded peptides,play critical roles in fundamental biological *** this study,we developed an integrated peptidogenomic pipeline using high-throughput mass spectra to probe a customized six-frame translation database and applied it to large-scale identification of NCPs in plants.A total of 1993 and 1860 NCPs were unambiguously identified in maize and Arabidopsis,*** NCPs showed distinct characteristics compared with conventional peptides and were derived from introns,3′UTRs,5′UTRs,junctions,and intergenic ***,our results showed that translation events in unannotated transcripts occur more broadly than previously *** addition,we found that dozens of maize NCPs are enriched within regions associated with phenotypic variations and domestication selection,indicating that they potentially are involved in genetic regulation of complex traits and domestication in *** together,our study developed an integrated peptidogenomic pipeline for large-scale identification of NCPs in plants,which would facilitate global characterization of NCPs from other *** identification of large-scale NCPs in both monocot(maize)and dicot(Arabidopsis)plants indicates that a large portion of plant genome can be translated into biologically functional molecules,which has important implications for functional genomic studies.

关键词： non-conventional peptides small open reading frames peptidogenomics mass spectrometry six-frame translation plants

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The antioxidant protein ZmPrx5 contributes resistance to maize stalk rot

引用

The Crop Journal 2022年第4期10卷 1049-1058页

作者： Shunxi Wang Wencheng Liu Zan Chen Jinghua Zhang Xingmeng Jia Mingyue Gou Xueyan Chen Yuqian Zhang Hehuan Li Yanhui Chen liuji wu National Key Laboratory of Wheat and Maize Crop Science College of AgronomyHenan Agricultural UniversityZhengzhou 450002HenanChina State Key Laboratory of Crop Stress Adaptation and Improvement State Key Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifeng 475004HenanChina School of Environmental and Rural Science University of New EnglandArmidaleNSW 2351Australia

Maize(Zea mays L.)stalk rot is a devastating disease worldwide,causing severe yield *** previous studies have focused on the genetic dissection of maize resistance to stalk rot,the mechanisms of resistance remain largely *** used a comparative proteomics approach to identify candidate proteins associated with stalk rot *** analyses revealed 763 proteins differentially accumulated between Fusarium graminearum and mock-inoculated *** them,the antioxidant protein ZmPrx5,which was up-accumulated in diseased plants,was selected for further ***5 transcripts were present in root,stalk,leaf,ear,and reproductive *** expression of ZmPrx5 in three inbred lines increased significantly upon *** ***5 was localized in the *** to control plants,maize plants overexpressing ZmPrx5 showed increased resistance to *** infection,and ZmPrx5 mutant plants were more susceptible than wild-type ***-associated pathways including plant–pathogen interactions,phenylalanine metabolism,and benzoxazinoid and flavonoid biosynthesis were suppressed in ZmPrx5 homozygous mutant plants compared with wild-type *** suggest that ZmPrx5 positively regulates resistance against stalk rot in maize,likely through defense-oriented transcriptome *** results lay a foundation for further research on the roles of Prx5 subfamily proteins in resistance to plant fungal diseases,and provide a potential genetic resource for breeding disease-resistance maize lines.

关键词： Maize stalk rot Zmprx5 Transgenic Mutant Disease resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

O2-ZmGRAS11 coordinates cell expansion and endosperm filling

引用

Molecular Plant 2022年第2期15卷 212-212页

作者： Chun-Lin Shi liuji wu National Key Laboratory of Wheat and Maize Crop Science College of AgronomyHenan Agricultural UniversityZhengzhou 450046China ANGENOVO 1414 VikenNorway

Maize(Zea mays),a major crop for food and feed,is grown world-wide with a high annual *** grain size and weight of maize are mainly determined by starch and storage proteins that are actively synthesized during endosperm *** early endo-sperm development,the maize endosperm undergoes a series of cell division,differentiation,and expansion *** division and expansion determine the number and size of endosperm cells,respectively,influencing the final grain size and *** cell division mainly occurs during early endosperm development,cell expansion occurs continuously,particularly during the endo-sperm filling stage when starchy endosperm cells are enlarged with storage *** several key genes involved in the regulation of either endosperm filling or cell expansion being identified,how these two processes are coordinated remains elusive.

关键词： mays expansion filling

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Senome-wide Association Studies in Maize： Praise and Stargaze

引用

Molecular Plant 2017年第3期10卷 359-374页

作者： Yingjie Xiao Haijun Liu liuji wu Marilyn Warburton Jianbing Yan National Key Laboratory of Crop Genetic Improvement Huaz.hong Agricultural University Wuhan 430070 China Synergetic Innovation Center of Henan Grain Crops Henan Agricultural University Zhengzhou 450002 China United States of Department of Agriculture Agricultural Research Service Corn Host Plant Resistance Research Unit Box 9555 MS 39762 Mississippi USA These authors contributed equally to this article

Genome-wide association study （GWAS） has become a widely accepted strategy for decoding genotype- phenotype associations in many species thanks to advances in next-generation sequencing （NGS） technol- ogies. Maize is an ideal crop for GWAS and significant progress has been made in the last decade. This review summarizes current GWAS efforts in maize functional genomics research and discusses future prospects in the omics era. The general goal of GWAS is to link genotypic variations to corresponding dif- ferences in phenotype using the most appropriate statistical model in a given population. The current re- view also presents perspectives for optimizing GWAS design and analysis. GWAS analysis of data from RNA, protein, and metabolite-based omics studies is discussed, along with new models and new popula- tion designs that will identify causes of phenotypic variation that have been hidden to date. The joint and continuous efforts of the whole community will enhance our understanding of maize quantitative traits and boost crop molecular breeding designs.

关键词： GWAS omics mixed model population design functional genomics Zea mays

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ligand deprivation activates receptor signaling

引用

Molecular Plant 2022年第12期15卷 1827-1827页

作者： Chun-Lin Shi liuji wu National Key Laboratory of Wheat and Maize Crop Science College of AgronomyHenan Agricultural UniversityZhengzhou 450046China ANGENOVO 1414 VikenNorway

Signaling peptides,together with their receptor kinases and proteins,are involved in a wide range of processes related to plant growth,development,and stress *** Arabidopsis,over 1000 different peptides and 251 leucin... 详细信息

关键词： Arabidopsis stress plant

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ascorbate peroxidase 1 confers resistance to southern corn leaf blight in maize

引用

Journal of Integrative Plant Biology 2022年第6期64卷 1196-1211页

作者： Jinghua Zhang Xingmeng Jia Guan-Feng Wang Shijun Ma Shunxi Wang Qin Yang Xueyan Chen Yuqian Zhang Yajing Lyu Xiaoxu Wang Jiawei Shi Yangtao Zhao Yanhui Chen liuji wu National Key Laboratory of Wheat and Maize Crop Science Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China The Key Laboratory of Plant Development and Environmental Adaptation Biologym Ministry of EducationSchool of Life SciencesShandong UniversityQingdao 266237China State Key Laboratory of Crop Stress Biology in Arid Areas College of AgronomyNorthwest A&F UniversityYangling 712100China School of Environmental and Rural Science University of New EnglandArmidale 2351NSWAustralia

Southern corn leaf blight (SCLB), caused by Bipolarismaydis, is one of the most devastatingdiseases affecting maize production. However,only one SLCB resistance gene, conferring partialresistance, is currently known, underscoring theimportance of isolating new SCLB resistancerelatedgenes. Here, we performed a comparativeproteomic analysis and identified 258 proteinsshowing differential abundance during the maizeresponse to B. maydis. These proteins included anascorbate peroxidase (Zea mays ascorbate peroxidase1 (ZmAPX1)) encoded by a gene locatedwithin the mapping interval of a previously identifiedquantitative trait locus associated with SCLBresistance. ZmAPX1 overexpression resulted inlower H_(2)O_(2) accumulation and enhanced resistanceagainst B. maydis. Jasmonic acid (JA)contents and transcript levels for JA biosynthesisand responsive genes increased in ZmAPX1-overexpressing plants infected with B. maydis,whereas Zmapx1 mutants showed the oppositeeffects. We further determined that low levels of H_(2)O_(2) are accompanied by an accumulation of JAthat enhances SCLB resistance. These resultsdemonstrate that ZmAPX1 positively regulatesSCLB resistance by decreasing H_(2)O_(2) accumulationand activating the JA-mediated defensesignaling pathway. This study identified ZmAPX1as a potentially useful gene for increasing SCLBresistance. Furthermore, the generated datamay be relevant for clarifying the functions ofplant APXs.

关键词： ascorbate peroxidase comparative proteomics disease resistance jasmonic acid maize southern corn leaf blight

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

融合注意力机制的改进Mask-RCNN遥感影像建筑物提取

引用

测绘科学 2024年第1期49卷 79-89页

作者：李健庞留记吴浩王心宇郑州大学地球科学与技术学院郑州450000 华中师范大学城市与环境科学学院武汉430000

针对复杂影像背景和密集建筑物堆叠导致建筑物提取效果不完整,存在误检、漏检等问题,提出了一种改进Mask-RCNN遥感影像建筑物提取方法。利用双通道注意力机制增强目标的有效特征,同时引入特征增强金字塔网络增强网络对遥感影像的上下文... 详细信息

针对复杂影像背景和密集建筑物堆叠导致建筑物提取效果不完整,存在误检、漏检等问题,提出了一种改进Mask-RCNN遥感影像建筑物提取方法。利用双通道注意力机制增强目标的有效特征,同时引入特征增强金字塔网络增强网络对遥感影像的上下文特征信息地提取能力,结合双通道下采样模块减少特征损失,提高模型提取的精度和效率。实验表明,提出的改进Mask-RCNN在建筑物数据集和RSOD数据集上,与多种方法进行实验对比验证,Precision和F1值均高于对比方法,且目标识别的结果更加完整,目标漏检率更低。

关键词：双通道注意力机制改进Mask-RCNN网络多尺度特征金字塔建筑物提取遥感影像

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

玉米自交系齐319对弯孢霉叶斑病抗性的遗传分析

引用

河南农业大学学报 2015年第2期49卷 234-238页

作者：刘翠娜代君丽吴刘记邢小萍李洪连袁虹霞河南农业大学植物保护学院河南郑州450002 河南农业大学农学院河南郑州450002

以玉米自交系齐319(抗)×昌7-2(感)组合的P1、P2、F1、F24个世代为材料,采用植物数量性状主基因+多基因混合遗传模型,分析了该组合苗期及成株期抗病遗传特点,揭示了齐319对玉米弯孢霉叶斑病的抗性遗传规律。结果表明:齐319苗期抗性遗传... 详细信息

以玉米自交系齐319(抗)×昌7-2(感)组合的P1、P2、F1、F24个世代为材料,采用植物数量性状主基因+多基因混合遗传模型,分析了该组合苗期及成株期抗病遗传特点,揭示了齐319对玉米弯孢霉叶斑病的抗性遗传规律。结果表明:齐319苗期抗性遗传符合2对加性-显性主基因+加性-显性多基因模型;成株期抗性遗传符合2对加性-显性主基因+加性-显性多基因模型。控制F2群体苗期与成株期抗性的主基因遗传率分别为65.14%和83.98%,表明齐319对弯孢霉叶斑病的抗性是两对主基因起主要作用。

关键词：玉米齐319 弯孢霉叶斑病抗性遗传分析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：