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Adenovirus-mediated and tumor-specific transgene expression of the sodium-iodide symporter from the human telomerase reverse transcriptase promoter enhances killing of lung cancer cell line in vitro

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Chinese Medical Journal 2010年第15期123卷 2070-2076页

作者： SHI Yi-zhen ZHANG Jun liu zeng-li DU Shou-ying SHEN Yong-mei Department of Nuclear Medicine the Second Affiliated Hospital of Soochow University Suzhou 215004 Jiangsu China Department of Nuclear Medicine Taizhou People's HospitalTaizhou 225300 Jiangsu China Department of Pathology University of California at San Diego Medical Center 200 West Arbor Drive San Diego CA92103 USA

Background The sodium-iodide symporter （NIS） protein can mediate the active radioiodine *** human telomerase reverse transcriptase （hTERT） promoter is known to be selectively reactivated in majority of tumors and hence could be used for tumor *** constructed a recombinant adenovirus containing the human sodium iodide symporter （hNIS） gene directed by the hTERT promoter, characterized the ability of infected cells in uptaking iodide, and explored the therapeutic efficacy of 131I in a lung cancer cell line in *** The hTERT promoter was amplified by PCR from DNA isolated from log-phase HepG2 cells, subcloned into lineralized FL＊-hNIS/pcDNA3, and then the hTERT-hNIS sequence was subcloned into the shuttle plasmid *** recombinant adenovirus Ad-hTERT-hNIS was constructed by AdEasy system.A positive control adenovirusAd-CMV-hNIS and a negative control adenovirus Ad-CMV were created similarly.A549 cells were transduced with recombinant adenoviruses.125I uptake studies and sodium perchlorate suppression studies were used to confirm hNIS expression and *** effects of 131I on tumor cells were studied by in vitro clonogenic *** We first successfully constructed an adenovirus mediated transgene expression system of the hNIS under the control of hTERT *** infected with recombinant adenovirus constructs expressing hNIS directed by hTERTand CMV-promoters （Ad-hTERT-hNIS and Ad-CMV-hNIS, respectively）, the lung cancer cell line A549 had increased ability to uptake radioiodide up to 23- and 30- fold compared to the control parental cells, *** radioiodide uptake ability of both the Ad-CMV-hNIS and Ad-hTERT-hNIS transduced cell lines were repressed 11-fold by sodium perchlorate （NaCIO4）.The subsequent in vitro clonogenic assay of the infected A549 cell line was further repressed to 23% （Ad-CMV-hNIS） and 30% （Ad-hTERT-hNIS） of the control group after receiving radioiodide for 7 hours （P 〈0.001）.Conclusion Our

关键词： sodium-iodide symporter infection adenoviridae iodine radioisotopes colony-forming units assay

Biodistribution and preparation of technetium-99m-labeled D-D3 monoclonal antibody against pro-gastrin-releasing peptide ~31-98） in mice

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Chinese Medical Journal 2013年第7期126卷 1333-1336页

作者： HA0 li-jun HONG Zhi-hui SHI Yi-zhen liu zeng-li ZHOU Xiao-lin Department of Ultrasound Jiangsu China Department of Radiology and Nuclear Medicine Chinese Institute for Radiation Protection Taiyuan Shanxi 030006 China Department of Nuclear Medicine Second Affiliated Hospital of Soochow University Suzhou 215004 Jiangsu China Department of Radiology and Nuclear Medicine Chinese Institute for Radiation Protection Taiyuan Shanxi 030006 China

Background We previously reported that iodine-131（131^I）-Iabeled anti-pro-gastrin-releasing peptide （ProGRP（31-98）） monoclonal antibody D-D3 could selectively accumulate in the tumor sites of nude mice bearing small cell lung cancer （SCLC） xenografts. However, 1311-D-D3 was cleared slowly from the body, and the best radioimmunoimaging time for SCLC was 72-96 hours after injection. The aims of this study were to radiolabel anti-ProGRP（31-98） D-D3 monoclonal antibody with technetium-99m（99m^Tc） and to investigate the biodistribution of this antibody in healthy ICR mice. Methods D-D3was labeled with 99m^Tc via the 2-mercaptoethanol reduction method. 99m^Tc-D-g3 was purified by the gel column separation method. The labeling efficiency and radiochemical purity were measured by thin-layer chromatography. The immunological activity of 99m^Tc-D-O3 was determined with cell conjugation assays. 99m^Tc-D-D3 was injected into healthy ICR mice via a tail vein, and all the healthy ICR mice were sacrificed by cervical dislocation at a designated time. Then, the blood and major organs were removed and weighed, and counted in a gamma scintillation counter to determine the percentage of the injected dose per gram （%ID/g）. Results The labeling rate and the radiochemical purity of 99m^TC-D-D3 were （73.87±2.89）% and （94.13±4.49）%, respectively. The immunobinding rates of 99m^Tc-O-D3 to the human small cell lung cancer NCI-H446 cell line and lung adenocarcinoma A549 cell line were （81.2±2.37）% and （24.3±1.46）%, respectively. The distribution data of normal ICR mice demonstrated that 99m^TC-D-D3was mainly distributed in the liver, kidney and lung, and less in the brain tissue and muscle. Conclusions 99m^Tc-D-D3 antibody not only had high radiochemical purity, but also had good stability both in vitro and in vivo, and maintained good immunological activity. 99m^Tc-D-D3 was metabolized mainly in the kidney and liver, and the blood radioactivity decreased rapidly. Thus, 99m^Tc-O-D3 is conduci

关键词： 99m^Tc-D-D3 monoclonal antibody pro-gastrin-releasing peptide(31-98)

Boosting hydrocarbon conversion via Cu-doping induced oxygen vacancies on CeO2 in CO2 electroreduction

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Journal of Energy Chemistry 2025年第1期100卷 66-76页

作者： Lei Xue Tong Shi Chenhui Han Heng Zhang Fenrong liu Haorun li Yan Wang Xiaojun Gu Shanghong zeng Inner Mongolia Key Laboratory of Chemistry and Physics of Rare Earth Materials School of Chemistry and Chemical Engineering Inner Mongolia University State Key Laboratory of Coal Conversion Institute of Coal Chemistry Chinese Academy of Sciences Institute of Catalysis Department of Chemistry Zhejiang University National Key Laboratory of Baiyunobo Rare Earth Resource Research and Comprehensive Utilization Baotou Research Institute of Rare Earths

Conversion of CO2back to hydrocarbons is the most direct way of closing the“carbon cycle”,and its significance is further enlarged if this process is driven by renewable energies such as ***,precisely controlling the product selectivity towards hydrocarbons against the competitive hydrogen evolution remains challenging,especially for Cu-based ***,we report a novel defect engineering strategy,by which Cu-doping-induced oxygen vacancies on CeO2nanorods were effectively created,with adjustable vacancy/Cu *** resulting optimum catalyst shows up to 79% catalytic current density to hydrocarbons （excluding CO）,with 49% faradaic efficiency to *** and density functional theory unveil that the ratio between oxygen vacancy and Cu affects significantly the formation of*CHO and activation of H2O,which leads to the following deep hydrogenation to *** findings may spur new insights for designing and developing more controllable chemical process relevant to CO2utilization.

关键词： Oxygen vacancy Hydrocarbon production Reaction mechanism

Repetitive traumatic brain injury–induced complement C1–related inflammation impairs long-term hippocampal neurogenesis

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Neural Regeneration Research 2025年第3期20卷 821-835页

作者： Jing Wang Bing Zhang Lanfang li Xiaomei Tang Jinyu zeng Yige Song Chao Xu Kai Zhao Guoqiang liu Youming Lu Xinyan li Kai Shu Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Neurosurgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Pathophysiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Institute for Brain Research Wuhan Center of Brain ScienceHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Graduate Student Chongqing Medical UniversityChongqingChina Department of Basic Medicine School of Medical ScienceHubei University for NationalitiesEnshiHubei ProvinceChina Department of Anatomy School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Repetitive traumatic brain injury impacts adult neurogenesis in the hippocampal dentate gyrus,leading to long-term cognitive ***,the mechanism underlying this neurogenesis impairment remains *** this study,we established a male mouse model of repetitive traumatic brain injury and performed long-term evaluation of neurogenesis of the hippocampal dentate gyrus after repetitive traumatic brain *** results showed that repetitive traumatic brain injury inhibited neural stem cell proliferation and development,delayed neuronal maturation,and reduced the complexity of neuronal dendrites and *** with repetitive traumatic brain injuryalso showed deficits in spatial memory ***,following repetitive traumatic brain injury,neuroinflammation was enhanced in the neurogenesis microenvironment where C1q levels were increased,C1q binding protein levels were decreased,and canonical Wnt/β-catenin signaling was *** inhibitor of C1 reversed the long-term impairment of neurogenesis induced by repetitive traumatic brain injury and improved neurological *** findings suggest that repetitive traumatic brain injury–induced C1-related inflammation impairs long-term neurogenesis in the dentate gyrus and contributes to spatial memory retrieval dysfunction.

关键词： complement C1 dendrite dentate gyrus hippocampus neural stem cell neurogenesis neuroinflammation neurological function neuron traumatic brain injury

A Modified Conjugate Gradient Method with Global Convergence Property

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Journal of Mathematical Research and Exposition 2009年第1期29卷 65-75页

作者： HONG ling MO li liu WEI zeng Xin College of Mathematics & Information Science Guangxi University Guangxi 530004 China

A new conjugate gradient method is proposed in this paper. For any （inexact） line search, our scheme satifies the sufficient descent property. The method is proved to be globally convergent if the restricted Wolfe-P... 详细信息

关键词： unconstrained optimization conjugate gradient method Wolfe-Powell line search global convergence.

Effect of Chemical Doping and Ion Implantation on Cond uctivity of Poly(p-phenylene vinylene) Derivatives

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Semiconductor Photonics and Technology 2005年第3期11卷 188-191页

作者： li Bao-ming WU Hong-cai liu Xiao-zeng li Xiao-qi GAO Chao Institute of Photoelectric Technol. and Solar Energy School of Electron. and Inform. Eng. Xi＇an Jiaotong University Xi＇an 710049 CHN

The surface conductivity of poly [ 2-methoxy-5-（3＇-methyl） butoxy]-p-phenylene vinylene （PMOMBOPV） films doped with FeCl3 and H2SO4 by chemical method and implanted by N^＋ ions was studied and the comparison of environmental stability of conductive behavior was also investigated. The energy and dose of N^＋ ions were in the rang 15～35 keV and 3. 8×10^15～9. 6×10^16 ions/cm^2, respectively. The conductivity of PMOMBOPV film was enhanced remarkably with the increases of the energy and dose of N^＋ ions. For example, the conductivity of PMOMBOPV film was 3. 2×10^-2S/cm when ion implantation was performed with an energy of 35 keV at a dose of 9. 6 × 10^14 ions/cm^2 , which was almost seven orders of magnitude higher than that of film unimplanted. The environmental stability of conductive behavior for ionimplanted film was much better than that of chemical doped films. Moreover, the conductive activation energy of ion-implanted films was measured to be about 0.17 eV.

关键词： Ion implantation Chemical doping Poly[2-methoxy-5-(3'-methyl)butoxy]-p-phenylene vinylene Surface conductivity Conductive activation energy

liver as a new target organ in Alzheimer's disease:insight from cholesterol metabolism and its role in amyloid-beta clearance

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Neural Regeneration Research 2025年第3期20卷 695-714页

作者： Beibei Wu Yuqing liu Hongli li Lemei Zhu lingfeng zeng Zhen Zhang Weijun Peng Department of Integrated Traditional Chinese&Western Medicine The Second Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Academician Workstation Changsha Medical UniversityChangshaHunan ProvinceChina Yangsheng College of Traditional Chinese Medicine Guizhou University of Traditional Chinese MedicineGuiyangGuizhou ProvinceChina Qinhuangdao Shanhaiguan Pharmaceutical Co. LtdQinhuangdaoHebei ProvinceChina National Clinical Research Center for Mental Disorder The Second Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina

Alzheimer's disease,the primary cause of dementia,is characterized by neuropathologies,such as amyloid plaques,synaptic and neuronal degeneration,and neurofibrillary *** amyloid plaques are the primary characteristic of Alzheimer's disease in the central nervous system and peripheral organs,targeting amyloid-beta clearance in the central nervous system has shown limited clinical efficacy in Alzheimer's disease *** abnormalities are commonly observed in patients with Alzheimer's *** liver is the primary peripheral organ involved in amyloid-beta metabolism,playing a crucial role in the pathophysiology of Alzheimer's ***,impaired cholesterol metabolism in the liver may exacerbate the development of Alzheimer's *** this review,we explore the underlying causes of Alzheimer's disease and elucidate the role of the liver in amyloid-beta clearance and cholesterol ***,we propose that restoring normal cholesterol metabolism in the liver could represent a promising therapeutic strategy for addressing Alzheimer's disease.

关键词： ABCA1 Alzheimer's disease amyloid-beta apolipoprotein E cholesterol metabolism liver liver X receptor low-density lipoprotein receptor-related protein 1 peripheral clearance tauroursodeoxycholic acid

基于动态CT识别的冻土单轴压缩损伤本构模型

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岩土力学 2005年第4期26卷 542-546页

作者：刘增利张小鹏李洪升大连理工大学工程力学系工业装备结构分析试验室

采用连续介质力学与热力学方法,建立了冻土单轴压缩损伤本构模型。以兰州冻结黄土为例,基于冻土单轴压缩动态CT试验,对冻土附加损伤的2个阶段,即塑性损伤和微裂纹扩展损伤分别采用硬化曲线法和动态CT识别方法进行了损伤计算,在此基础上... 详细信息

采用连续介质力学与热力学方法,建立了冻土单轴压缩损伤本构模型。以兰州冻结黄土为例,基于冻土单轴压缩动态CT试验,对冻土附加损伤的2个阶段,即塑性损伤和微裂纹扩展损伤分别采用硬化曲线法和动态CT识别方法进行了损伤计算,在此基础上给出了冻土附加损伤与弹性应变的对应关系,建立了基于试验的冻土损伤演化方程,并进行了有效应力的计算。

关键词：冻土单轴压缩 CT动态测试损伤本构模型

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原位冻结黏土单轴压缩试验研究

岩土力学 2007年第12期28卷 2657-2660页

作者：刘增利张小鹏李洪升大连理工大学工程力学系工业装备结构分析实验室

采用沈阳原位冻结黏土制备了冻土单轴压缩试样,进行了冻结黏土的单轴压缩试验,获得了冻结黏土单轴压缩载荷-位移关系曲线,并对原位冻结黏土的破坏特征进行了研究。试验表明,原位冻结黏土在给定温度(-15℃)和加载速率范围(2.5×10-2～1.0... 详细信息

采用沈阳原位冻结黏土制备了冻土单轴压缩试样,进行了冻结黏土的单轴压缩试验,获得了冻结黏土单轴压缩载荷-位移关系曲线,并对原位冻结黏土的破坏特征进行了研究。试验表明,原位冻结黏土在给定温度(-15℃)和加载速率范围(2.5×10-2～1.0×10-5 mm/s)下,开始均具有一定的线弹性压缩过程,随后产生微裂纹,而冻土也进入塑性硬化阶段,随着裂纹的扩展与贯通,最终达到剪切破坏。原位冻土的破坏形型表明,由于其固有的结构性,使之与重塑土的单轴压缩破坏特征具有明显的不同。

关键词：原位冻结黏土单轴压缩破坏特征