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Comparison of conventional and directional freezing for the cryopreservation of human umbilical vein endothelial cells

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International Journal of Ophthalmology(English edition) 2014年第5期7卷 768-772页

作者： Bing Qi qing-Shan Ji Guang-Hui Hou liu Li Xian-Fen Cao Jing Wu Department of Ophthalmology Zhuhai People's Hospital the Third Affiliated Hospital of Jinan University Department of Ophthalmology the First Affiliated Hospital of Jinan University Department of Ophthalmology Affiliated Anhui Provincial Hospital of Anhui Medical University Department of Ophthalmology the First Hospital of Nanchang the Third Affiliated Hospital of Nanchang University

AIM:To compare conventional slow equilibrium cooling and directional freezing(DF) by gauze package for cryopreservation of human umbilical vein endothelial cells(HUVECs).METHODS:HUVECs were randomly assigned to conventional freezing(CF) and DF by gauze package group. The two groups of HUVECs were incubated with a freezing liquid consisting of 10% dimethylsulfoxide(DMSO), 60% fetal bovine serum(FBS) and 30%Dulbecco’s modified Eagle’s medium(DMEM) and then put into cryopreserved tubes. CF group, slow equilibrium cooling was performed with the following program:precool in 4℃ for 30 min,-20℃ for 1h, and then immersion in-80℃ refrigerator. DF group, the tubes were packaged with gauze and then directional freezing in-80℃ refrigerator straightly. One month later, the vitality of HUVECs were calculated between two ***:There was no significant difference in the survival rate and growth curve between CF and DF groups. The DF group was significantly better than CFgroup in adherent rates, morphological changes and proliferative ***:In the conventional cryopreserved method, cells are slow equilibrium cooling by steps(4℃,-20℃ and finally-80℃), which is a complicated and time-consuming process. But the improved DF by gauze package method is better than conventional method, for which is convenient and easy to operate.

关键词： cryopreservation human umbilical vein endothelial cells slow equilibrium cooling directional freezing

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Exploration and Practice of Student Engineering Competency Training in University-Industry Collaboration Model

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计算机教育 2020年第12期 11-19页

作者： Junwei Du quan liu Guozhu liu Meijiao Xu Xinglu Ma College of Information Science and Technology Qingdao University of Science and TechnologyQingdao 266061China qingdao Software Training Education Technology Co. LtdQingdao 266000China.

Engineering competency is a basic and important quality of being an engineer,but it is one of the weak points in today’s higher engineering *** industrial development needs as the goal of personnel training and focusing on the cultivation of engineering competency,the authors design an integrated practical teaching system,which is based on the requirements for engineering and technical professionals and the characteristics of qingdao University of Science and Technology(QUST).The system adopts a“project driven”teaching method and a“swimming poolTM”training mode and establishes a teaching,administration,feedback and evaluation platform consistent with the implementation of this practical teaching mode,so as to ensure the quality of engineering and technical professionals training in an in-depth university-industry collaboration mode.

关键词： engineering and technical professionals talent cultivation engineering competency university-industry collaboration practical teaching

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Effects of Fuzhenghuayu decoction on collagen synthesis of cultured hepatic stellate cells,hepatocytes and fibroblasts in rats

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World Journal of Gastroenterology 1998年第6期4卷 93-94页

作者： liu Cheng 1, liu Ping 1, liu Cheng Hai 1, ZHU Xiu qing 1 and JI Guang 1

AIM To study the mechanism of Fuzhenghuayu (FZHY) decoction on anti liver fibrosis. METHODS FZHY 10% decoction sera was incubated with rat normal subcultured hepatic stellate cells (HSC) and fibrotic primarily cultured HSC, normal and fibrotic hepatocytes and subcultured skin fibroblasts separately. Cell intracellular and extracellular collagen synthesis rates were measured by the method of Proline impulse and collagenase digestion. RESULTS For primarily cultured HSC and hepatocytes, both of intracellular and extracellular collagen synthesis rates decreased in the drug sera group. For the normal subcultured HSC and primarily cultured hepatocytes, the extracellular collagen secretion was decreased obviously by the drug sera, and intracellular collagen synthesis rates were inhibited to some extents. For fibroblasts, both intracellular and extracellular collagen synthesis rates were inhibited some what, but no significant differences were found. CONCLUSION The mechanism of FZHY decoction on anti liver fibrosis may be associated with inhibition of liver collagen production.

关键词： Fuzhenghuayu decoction collagen synthesis hepatic stellate cells hepatocytes fibroblasts

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The sugar transporter system of strawberry:genome-wide identification and expression correlation with fruit soluble sugar-related traits in a Fragaria×ananassa germplasm collection

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Horticulture Research 2020年第1期7卷 1303-1321页

作者： Hai-Ting liu Ying Ji Ya liu Shu-Hua Tian qing-Hua Gao Xiao-Hua Zou Jing Yang Chao Dong Jia-Hui Tan Di-An Ni Ke Duan Shanghai Key Laboratory of Protected Horticultural Technology Forestry and Fruit Tree Research InstituteShanghai Academy of Agricultural Sciences(SAAS)Shanghai 201403China Ecological Technique and Engineering College Shanghai Institute of TechnologyShanghai 201418China Environmental Engineering College Suzhou Polytechnic Institute of AgricultureSuzhou 215008China

Sugar from plant photosynthesis is a basic requirement for life *** transporters are the proteins that mediate sugar allocation among or within source/sink *** transporters of the major facilitator superfamily(MFS)targeting carbohydrates represent the largest family of sugar transporters in many ***(Fragaria×ananassa Duchesne)is an important crop appreciated worldwide for its unique fruit *** involvement of MFS sugar transporters(STs)in cultivated strawberry fruit sugar accumulation is largely *** this work,we characterized the genetic variation associated with fruit soluble sugars in a collection including 154 ***,a total of 67 ST genes were identified in the v4.0 genome integrated with the v4.0.a2 protein database of ***,the dominant subgenome provider for modern cultivated *** analysis updated the nomenclature of strawberry ST *** the chromosomal distribution and structural characteristics of the ST family were ***-RT-PCR analysis in nine tissues from *** screened 34 highly expressed ST genes in *** three varieties with dramatically differing fruit sugar levels,qPCR integrated with correlation analysis between ST transcript abundance and sugar content identified 13 sugar-correlated *** correlations were re-evaluated across 19 varieties,including major commercial cultivars grown in ***,a model of the contribution of the sugar transporter system to subcellular sugar allocation in strawberry fruits was *** work highlights the involvement of STs in controlling strawberry fruit soluble sugars and provides candidates for the future functional study of STs in strawberry development and responses and a new approach for strawberry genetic engineering and molecular breeding.

关键词： straw Fragaria Sugar

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TWO-YEAR OBSERVATION OF A RANDOMIZED TRIAL ON TACROLIMUS-BASED THERAPY WITH WITHDRAWAL OF STEROIDS OR MYCOPHENOLATE MOFETIL AFTER RENAL TRANSPLANTATION

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Chinese Medical Sciences Journal 2008年第4期23卷 244-248页

作者： qing-guo Zhu Ya-kun Zhao Wei liu Hui Luo Yu Qiu Zhi-zhong Gao Department of Urinary Surgery the Second Affiliated Hospital of Harbin Medical University Harbin 150086

Objective To evaluate the safety and feasibility of steroid or mycophenolate mofetil （MMF） withdrawal from tacrolimus-based immunosuppressant regimen in renal allograft recipients. Methods A cohort of 45 patients following cadaveric renal allograft transplantation were randomly divided into 3 groups based on the regimen of combination of tacrolimus, steroid, and MMF： triple therapy group, steroid withdrawal group, and MMF withdrawal group. During 2 years, survival of patients and allografts, clinical acute rejection, adverse events, hepatic and renal allograft function, and blood lipids were monitored to evaluate the safety and feasibility of steroid or MMF withdrawal after renal transplantation. Results During two-year observation, steroid or MMF was successfully withdrawn from immunosuppressant regimen based on tacrolimus without any clinical acute rejection renal allografts kept excellent function. Some adverse events among groups. Patient and graft survival rates were 100% and all the occurred and there were no significant differences Conclusion Withdrawal of steroid or MMF in low-immunological-risk renal allografts treated with tacrolimus-based immunosuppressant regimen can be achieved with no increased risk of acute rejection.

关键词： renal transplantation tacrolimus mycophenolate mofetil steroid

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论个人信息处理的合法、正当、必要原则

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法学家 2021年第5期 1-15,191页

作者：刘权中央财经大学法学院

"合法、正当、必要原则"已成为个人信息处理的基本原则。个人信息保护的告知同意机制日益流于形式,而且存在适用例外,在个人信息权利化存在困境的现实情境下,"合法、正当、必要原则"可以实现个人信息保护与合理利用的平衡。"合法原则"... 详细信息

"合法、正当、必要原则"已成为个人信息处理的基本原则。个人信息保护的告知同意机制日益流于形式,而且存在适用例外,在个人信息权利化存在困境的现实情境下,"合法、正当、必要原则"可以实现个人信息保护与合理利用的平衡。"合法原则"属于形式合法性范畴。"正当原则"要求个人信息处理必须出于特定、明确、合理的目的。为了实现个人信息社会价值的最大化,应允许个人信息处理者在符合个人合理预期的条件下,适当变更原初目的。"必要原则"包括禁止过度损害和禁止保障不足两大方面。它既要求处理个人信息时应在有助于目的实现的必要范围内运用最小损害的手段,又要求采取必要措施最大程度保障个人信息安全。"正当、必要原则"有利于弥补数字时代意思自治与契约自由的缺陷,可以矫正个人信息处理者同个人之间日益严重失衡的不平等态势,实际上是比例原则在私法中的体现。

关键词：个人信息处理合法、正当、必要原则个人信息保护比例原则

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Hepatitis C virus infections and genotypes in China

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Hepatobiliary & Pancreatic Diseases International 2002年第2期1卷 194-201页

作者： Yuan-Ding Chen Ming-Ying liu Wen-Lin Yu Jia-Qi Li Mei Peng qing Dai Xiao liu Zhen-quan Zhou From the Central Laboratory, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China Kunming Hospital for Infectious Diseases, Kunming 650041, China Central Laboratory Institute of Medical Biology Chinese Academy of Medical Sciences & Peking Union Medical College Kunming 650118 China. chenyd@***

Objective: To define the conditions of hepatitis C vi- rus (HCV) infections, and geographic and demo- graphic distributions of genotypes in China. Methods: HCV infected patients were selected from individuals with different patterns of liver diseases and high risk populations in different parts of China. Genotypes of HCV in some isolates were further ana- lyzed, based on the data from our laboratory studies and some carefully selected published literatures. Results: The anti-HCV positive rates were 9.7% in patients with acute hepatitis, 13.3% in patients with chronic hepatitis, 18.3% in patients with hepatocel- lular carcinoma, 33.0% in patients with liver cirrho- sis, and 43.2% in patients with posttransfusional hepatitis (average, 16.2% in patients with liver di- seases). The anti-HCV positive rates in the high risk populations were 36.4% in leukemic patients, 43.0% in hemodialysis patients, 12.7% in blood do- nors, 64.1% in drug abusers, 13.1% in prostitutes, and 2.57% in naturally healthy people. At least 4 clades (clades 1, 2, 3 and 6) of HCV were found in China with different geographic and demographic distributions. Genotype 1b was the most widely dis- tributed genotype, and genotype 3 was mainly found in Yunnan Province, Southwest China. Conclusion: China has a high incidence of HCV in- fection. Our results will provide a strategic basis for diagnosis, treatment and possibly prophylaxis of he- patitis C virus diseases.

关键词： HCV infections genotypes China

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Gravity observations along the eastern margin of the Tibetan Plateau and an application to the Lushan MS 7.0 earthquake

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Earthquake Science 2013年第3期26卷 251-257页

作者： Wei-feng Liang Yun-feng Zhao Yun-ma Xu Yi-qing Zhu Shu-song Guo Fang liu Lian liu Second Monitoring Center China Earthquake Administration

This paper introduces relative and absolute gravity change observations in the eastern portion of the Tibetan Plateau. We analyze and discuss a change that occurred in 2010 in the gravity along the eastern margin of the plateau and the relationship between this change and the 2013 Lushan Ms7.0 earthquake. Our results show that：（1） before the Lushan MsT.0 earthquake, gravity anomalies along the eastern margin of the Tibetan Plateau changed drastically. The Lushan earthquake occurred at the bend of the high gradient zone of gravity var- iation along the southern edge of the Longmenshan fault zone. （2） The 2013 Lushan earthquake occurred less than 100 km away from the epicenter of the 2008 Wenchuan earthquake. Lushan and Wenchuan are located at the center of a four- quadrant section with different gravity anomalies, which may suggest that restoration after the Wenchuan earthquake may have played a role in causing the Lushan earthquake. （3） A medium-term prediction based on changes in gravity anoma- lies was made before the Lushan Ms7.0 earthquake, in par- ticular, a prediction of epicenter location.

关键词： The eastern margin of the TibetanPlateau Gravity observation Gravity changeLushan earthquake Medium-term prediction

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Impact of cigarette smoking on recurrence of hyperlipidemic acute pancreatitis

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World Journal of Gastroenterology 2017年第47期23卷 8387-8394页

作者： Jun-Xi Xiang Liang-Shuo Hu Peng liu Bo-Yan Tian qing Su Yi-Chun Ji Xu-Feng Zhang Xue-Min liu Zheng Wu Yi Lv Department of Hepatobiliary Surgery First Affiliated Hospital of Xi'an Jiaotong University Regenerative Medicine and Surgery Engineering Research Center of Shaanxi Province

AIM To investigate the impact of cigarette smoking on the recurrence rate and recurrence-free survival in patients with hyperlipidemic acute pancreatitis(HLAP).METHODS A total of 863 patients were admitted to our hospital for acute pancreatitis(AP) from January 2013 to March2016, of whom 88 diagnosed with HLAP were enrolled in this retrospective study. Demographic data, medical history, previous episodes of pancreatitis, consumption of alcohol and cigarettes, as well as biochemical and hematological data were carefully recorded for univariate and multivariate analyses. During followup, the information on current smoking status and recurrent AP was gathered. Recurrence-free survival(RFS) was calculated using the Kaplan-Meier method, and the differences between groups were compared using the log-rank *** No significant differences were observed between the three groups in age or medical history of hyperlipidemia, fatty liver, diabetes mellitus, hypertension, or AP. The current smokers had a remarkably higher recurrence rate and a greater incidence of repeated episodes of AP(50.0% and 77.8%, respectively) than non-smokers(9.8% and 39.0%), and these two percentages were reduced to 9.1% and 36.4% for patients who gave up smoking. The median follow-up time was 13.5 mo and HLAP recurred after hospital discharge in 23(26.1%) patients. Multivariate analysis identified current smoking(HR = 6.3, P = 0.020) as an independent risk factor contributing to HLAP recurrence. Current smokers had significantly worse RFS than non-smokers(23 mo vs 42 mo), but no significant difference was documented between ex-smokers(34 mo) and non-smokers. The RFS was not significantly different between light and heavy *** Smoking is associated with worse RFS and an increased rate of HLAP recurrence. Continued smoking correlates with a compromised survival and smoking cessation should be recommended.

关键词： Acute pancreatitis Hyperlipemia Smoking Recurrence Epidemiology

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Clinical characteristics of patients with COVID-19 presenting with gastrointestinal symptoms as initial symptoms: Retrospective case series

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World Journal of Clinical Cases 2020年第14期8卷 2950-2958页

作者： Yun Yang Yuan-Chao Li Si-Cong Wang qing-qing Dai Xue-Song Jiang Shu Zuo liu Jia Jun-Bo Zheng Hong-Liang WangDepartment of Critical Care Medicine the Second Affiliated Hospital of Harbin Medical University Harbin 150001Heilongjiang ProvinceChina Department of Critical Care Medicine the Cancer Hospital of Harbin Medical UniversityHarbin 150081Heilongjiang ProvinceChina

BACKGROUND A large number of pneumonia cases due to coronavirus disease 2019(COVID-19)have been first reported in ***,the virus is sweeping all around the world and has infected millions of *** and pulmonary symptoms have been noticed as major and early signs of infection,whereas gastrointestinal symptoms were also observed in a significant portion of *** clinical investigation of disease onset was underestimated,especially due to the neglection of cases presenting with gastrointestinal *** To characterize the clinical features of coronavirus-infected patients with gastrointestinal symptoms as initial *** This is a retrospective,single-center case series of the general consecutive hospitalized patients with confirmed COVID-19 at Wuhan Union Hospital from February 2,2020 to February 13,*** to their initial symptoms,these patients were classified into two *** in group one presented with pulmonary symptoms(PS)as initial symptoms,and group two presented with gastrointestinal symptoms(GS).Epidemiological,demographic,clinical,laboratory,and treatment data were collected for *** Among the 50 patients recruited,no patient has been admitted to intensive care units,and no patient died during the *** duration of hospitalization was longer in the GS group than in the PS group(12.13±2.44 vs 10.00±2.13,P<0.01).All of the 50 patients exhibited decreased ***,lymphocytes in the GS group were significantly lower compared to those in the PS group(0.94±0.06 vs 1.04±0.15,P<0.01).Procalcitonin and hs-CRP were both significantly higher in the GS group than in the PS ***,the duration of viral shedding was significantly longer in the GS group compared to the PS group(10.22±1.93 vs 8.15±1.87,P<0.01).CONCLUSION COVID-19 patients presenting with gastrointestinal symptoms as initial symptoms need more days of viral shedding and hospitalization than the patients presenting

关键词： COVID-19 Initial symptoms Gastrointestinal symptoms Hospitalization Viral transmission

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