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New perspectives on the genetic structure of dotted gizzard shad(Konosirus punctatus)based on RAD-seq

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Marine Life Science & Technology 2024年第1期6卷 50-67页

作者： Ying Peng Yifan liu Jiasheng Li Kun Zhang Xun Jin Sixu Zheng Yunpeng Wang Zhenming Lu liqin liu Li Gong Bingjian liu National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization Zhejiang Ocean UniversityZhoushan 316022China National Engineering Research Center for Facilitated Marine Aquaculture Marine Science and Technology CollegeZhejiang Ocean UniversityZhoushan 316022China

To maintain,develop and rationally utilize marine organisms,understanding their genetic structure and habitat adaptation pattern is *** punctatus,which is a commercial fish species inhabiting the Indo-west Pacific Ocean,has shown an obvious annual global capture and aquaculture production decline due to climate changes and human *** the present study,restriction-site associated DNA sequencing(RAD-seq)was used to describe its genome-wide single nucleotide polymorphisms panel(SNPs).Among 146 individuals collected at nine locations scattered in China,Korea and Japan,a set of 632,090 SNPs were *** genetic analysis showed that *** individuals were divided into two significant genetic ***,potential genetic differentiation between northern and southern population of *** was *** results indicated that gene flow existed among sampling locations of ***,especially from southern Japan to ***,candidate genes associated with habitat adaptations of *** were identified,which are involved in diverse physiological processes of *** including growth and development(e.g.,KIDINS220,PAN3),substance metabolism(e.g.,PGM5)and immune response(e.g.,VAV3,CCT7,HSPA12B).Our findings may aid in understanding the possible mechanisms for the population genetic structure and local adaptation of ***,which is beneficial to establish the management and conservation units of ***,guiding the rational use of resources,with reference significance for a profound understanding of the adaptative mechanisms of other marine organisms to the environment.

关键词： Population structure Konosirus punctatus SNP RAD-seq Local adaptation

The Clausena lansium genome provides new insights into alkaloid diversity and the evolution of the methyltransferase family

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Journal of Integrative Agriculture 2024年第10期23卷 3537-3553页

作者： Yongzan Wei Yi Wang Fuchu Hu Wei Wang Changbin Wei Bingqiang Xu liqin liu Huayang Li Can Wang Hongna Zhang Zhenchang Liang Jianghui Xie State Key Laboratory of Biological Breeding of Tropical Crops Institute of Tropical Bioscience and BiotechnologyChineseAcademy of Tropical Agricultural SciencesHaikou 571101China Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables(Co-construction by Ministryand Province) Ministry of Agriculture and Rural Affairs/Institute of Tropical Fruit TreesHainan Academy of Agricultural SciencesHaikou 571100China Beijing Key Laboratory of Grape Sciences and Enology CAS Key Laboratory of Plant ResourcesInstitute of BotanyChineseAcademy of SciencesBeijing 100093China Key Laboratory of Tropical Fruit Biology(Ministry of Agriculture and Rural Affairs) South Subtropical Crops Research InstituteChinese Academy of Tropical Agricultural SciencesZhanjiang 524091China Haikou Experimental Station Chinese Academy of Tropical Agricultural SciencesHaikou 571101China Sanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry Hainan UniversityHaikou 570228China Sanya Research Academy Chinese Academy of Tropical Agricultural SciencesSanya 572019China

Wampee(Clausena lansium)is an important evergreen fruit tree native to southern China that has a long history of use for medicinal ***,a chromosome-level genome of *** was constructed with a genome size of 282.9 Mb and scaffold N50 of 30.75 *** assembled genome contains 48.70%repetitive elements and 24,381 protein-coding *** genomic analysis showed that *** diverged from Aurantioideae 15.91-24.95 million years ***,some expansive and specific gene families related to methyltransferase activity and S-adenosylmethionine-dependent methyltransferase activity were also *** analysis indicated that N-methyltransferase(NMT)is mainly involved in alkaloid biosynthesis and O-methyltransferase(OMT)participates in the regulation of coumarin accumulation in *** suggested that wampee's richness in alkaloids and coumarins might be due to the gene expansions of NMT and *** tandem repeat event was one of the major reasons for the NMT ***,the reference genome of *** will facilitate the identification of some useful medicinal compounds from wampee resources and reveal their biosynthetic pathways.

关键词： Clausena lansium genome evolution methyltransferase activity alkaloid biosynthesis coumarin accumulation

Two antibodies show broad,synergistic neutralization against SARS-CoV-2 variants by inducing conformational change within the RBD

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Protein & Cell 2024年第2期15卷 121-134页

作者： Hui Sun Tingting Deng Yali Zhang Yanling Lin Yanan Jiang Yichao Jiang Yang Huang Shuo Song Lingyan Cui Tingting Li Hualong Xiong Miaolin Lan liqin liu Yu Li Qianjiao Fang Kunyu Yu Wenling Jiang Lizhi Zhou Yuqiong Que Tianying Zhang Quan Yuan Tong Cheng Zheng Zhang Hai Yu Jun Zhang Wenxin Luo Shaowei Li Qingbing Zheng Ying Gu Ningshao Xia State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public HealthSchool of Life SciencesXiamen UniversityXiamen 361102China National Institute of Diagnostics and Vaccine Development in Infectious Diseases School of Public HealthSchool of Life SciencesXiamen UniversityXiamen 361102China State Key Laboratory of Vaccines for Infectious Diseases School of Public HealthSchool of Life SciencesXiamen UniversityXiamen 361102China Xiang An Biomedicine Laboratory Xiamen 361102China Institute for Hepatology National Clinical Research Center for Infectious DiseaseShenzhen Third People's HospitalSchool of MedicineSouthem University of Science and TechnologyShenzhen 518112China The Second Affliated Hospital School of MedicineSouthern University of Science and TechnologyShenzhen 518112China Research Unit of Frontier Technology of Structural Vaccinology Chinese Academy of Medical SciencesXiamen 361102China

Continual evolution of the severe acute respiratory syndrome coronavirus(SARS-CoV-2)virus has allowed for its gradual evasion of neutralizing antibodies(nAbs)produced in response to natural infection or *** rapid nature of these changes has incited a need for the development of superior broad nAbs(bnAbs)and/or the rational design of an antibody cocktail that can protect against the mutated virus ***,we report two angiotensin-converting enzyme 2 competing nAbs—8H12 and 3E2—with synergistic neutralization but evaded by some Omicron ***-electron microscopy reveals the two nAbs synergistic neutralizing virus through a rigorous pairing permitted by rearrangement of the 472-489 loop in the receptor-binding domain to avoid steric *** antibodies based on these two nAbs tremendously extend the neutralizing breadth and restore neutralization against recent variants including currently dominant ***,these findings expand our understanding of the potential strategies for the neutralization of SARS-CoV-2 variants toward the design of broad-acting antibody therapeutics and vaccines.

关键词： SARS-CoV-2 broad neutralizing antibody rearrangement synergistic neutralization

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《中国历史要籍介绍与选读》课程德育探究

西北成人教育学院学报 2024年第2期 59-64页

作者：刘丽琴兰州城市学院文史学院甘肃兰州730070

在当前德育建设、思政课程建设及新文科改革背景下,《中国历史要籍介绍与选读》课程作为大学历史教学的主要基础课程之一,所蕴含的大量经典的文献典籍为开展德育提供了丰富的素材。《中国历史要籍介绍与选读》课程所包含的德育素养主要... 详细信息

在当前德育建设、思政课程建设及新文科改革背景下,《中国历史要籍介绍与选读》课程作为大学历史教学的主要基础课程之一,所蕴含的大量经典的文献典籍为开展德育提供了丰富的素材。《中国历史要籍介绍与选读》课程所包含的德育素养主要包括培养学生的民族自豪感和文化自信心、正直无私、忠诚守信的道德品质等内容。这些素养是培育当代大学生爱国主义情怀和舍己为国家奉献的担当责任意识的主要元素。

关键词：大学历史课程德育

Wind-Vortex-Induced Vibrations of a Deepwater Jacket Pipe and Vibration Suppression Using a Nonlinear Energy Sink

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规则波浪中圆筒型浮式生产储装置参数激励非线性响应研究

中国海洋大学学报（自然科学英文版） 2024年第3期 633页

作者： liu liqin YU Yongjun CHEN Yiqun

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中国海洋大学学报（自然科学版） 2024年第8期54卷 132-141页

作者：刘利琴赵佳雯李焱余勇军天津大学水利工程仿真与安全国家重点实验室天津300350 中国船级社上海规范研究所上海200120

目前常见的提升圆筒型浮式生产储装置(Floating production storage and offloading vessels,FPSO)垂荡性能的方法是通过增加刚度使垂荡固有周期增大到20 s以上,远离常见波浪周期,但这使得垂荡固有周期与纵摇固有周期的比值接近1∶2,可... 详细信息

目前常见的提升圆筒型浮式生产储装置(Floating production storage and offloading vessels,FPSO)垂荡性能的方法是通过增加刚度使垂荡固有周期增大到20 s以上,远离常见波浪周期,但这使得垂荡固有周期与纵摇固有周期的比值接近1∶2,可能会导致参数激励现象的发生。因此本文针对此类圆筒型FPSO系统的参数激励非线性运动展开研究。考虑规则波建立了系统单自由度参数激励纵摇方程,本文中采用多尺度法求解运动方程的一次近似解析解,基于Lyapunov定理分析解的稳定性,以分析规则参数激励下圆筒型FPSO纵摇失稳的机理。最后,结合多尺度法与数值方法进行参数敏感性分析。结果表明,使参数激励周期与纵摇固有周期的比值远离1/2、降低参数激励幅值和增大纵摇阻尼的3种方式均能够有效避免大幅参数激励的发生。

关键词：圆筒型浮式生产储装置参数激励多尺度法失稳机理非线性响应参数敏感性分析

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数字韧性:风险社会下大学生必备的元素养

皖西学院学报 2024年第1期40卷 151-156页

作者：刘丽琴王志临安徽中医药高等专科学校基础教学部安徽芜湖241000 丽水学院教师教育学院浙江丽水323000

数字韧性是个体在遭遇数字技术风险时吸收干扰,规避、消解外在冲击和应激源的负面影响的风险管理能力,是风险社会下大学生必备的元素养。从风险社会视角,沿着信息—观念—价值—行为这一逻辑链分析了在数字社会中大学生面临的风险危机,... 详细信息

数字韧性是个体在遭遇数字技术风险时吸收干扰,规避、消解外在冲击和应激源的负面影响的风险管理能力,是风险社会下大学生必备的元素养。从风险社会视角,沿着信息—观念—价值—行为这一逻辑链分析了在数字社会中大学生面临的风险危机,剖析了数字韧性RARC特征和MI-AR-FR-PI框架,并依据特征和框架提出学校场域中抗逆、因应、复原、反弹的数字韧性提升路向,以期促进大学生健康合理地利用技术以适应数字社会。

关键词：数字风险数字韧性数字韧性教育

包容型领导在护士冲突与护理团队工作氛围间的调节作用

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护理研究 2024年第14期38卷 2514-2518页

作者：刘丽琴李美芩曹晓璇周明建阳泉市第一人民医院山西045000 哈尔滨工业大学(深圳)

目的:探讨护士冲突与服务氛围和安全氛围的关系以及包容型领导在其中的调节作用。方法:于2023年3月选取阳泉市5所医院的1 509名护士作为研究对象,采用护士一般资料调查表、护护关系量表、服务氛围量表、安全氛围量表、包容型领导量表进... 详细信息

目的:探讨护士冲突与服务氛围和安全氛围的关系以及包容型领导在其中的调节作用。方法:于2023年3月选取阳泉市5所医院的1 509名护士作为研究对象,采用护士一般资料调查表、护护关系量表、服务氛围量表、安全氛围量表、包容型领导量表进行调查。结果:1 509名护士的矛盾与冲突得分为(3.37±0.92)分,服务氛围得分为(5.15±1.00)分,安全氛围得分为(5.52±1.16)分,包容型领导得分为(5.05±1.18)分。相关性分析结果显示,护士冲突与服务氛围和安全氛围均呈负相关(P<0.05)。线性分层回归分析结果显示,包容型领导在护士冲突与服务氛围和安全氛围间具有调节作用(P<0.05)。结论:护士冲突对服务氛围和安全氛围有不良影响,包容型领导可以减弱该不良影响。

关键词：护士冲突服务氛围安全氛围包容型领导相关性

One-step synthesis of fluorescent hydroxyls-coated carbon dots with hydrothermal reaction and its application to optical sensing of metal ions

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Science China Chemistry 2011年第8期54卷 1342-1347页

作者： liu liqin LI YuanFang ZHAN Lei liu Yue HUANG ChengZhi Education Ministry Key Laboratory on Luminescence and Real-Time Analysis College of Chemistry and Chemical Engineering Southwest University Chongqing 400715 China CoUege of Pharmaceutical Sciences Southwest University Chongqing 400715 China

Carbon dots (CDs) with average diameter of 3.1 ± 0.5 nm were facilely synthesized with candle soot through hydrothermal reaction in sodium hydroxide aqueous solution. The as-prepared CDs were covered with a lot of hydroxyls, possessed properties of good water-solubility, anti-photobleaching, salt tolerance, and low cytotoxicity, and had a fluorescence quantum yield (QY) of about 5.5%. The fluorescence of the hydroxyls-coated CDs could be selectively quenched by metal ions such as Cr3+, Al3+ and Fe3+, which is because these metals can easily combine with the hydroxyl groups on the surface of CDs and induce aggregation of hydroxyls-coated CDs. Experiments showed that the quenching of Cr3+ had a Sterm-Volmer constant of 1.03 × 107 M-1 with a liner range of 1.0-25.0 μM and detection limit of 60 nM (3σ).

关键词： carbon dots fluorescence quantum yield cytotoxicity metal ions