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Broad-spectrum and powerful neutralization of bacterial toxins by erythroliposomes with the help of macrophage uptake and degradation

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Acta Pharmaceutica Sinica B 2022年第11期12卷 4235-4248页

作者： chunying liu Shuangrong Ruan Ying He Xuejing Li Yuefei Zhu Honglan Wang Hanwei Huang Zhiqing Pang School of Pharmacy Fudan UniversityKey Laboratory of Smart Drug DeliveryMinistry of EducationShanghai 201203China The Institute for Biomedical Engineering&Nano Science Tongji University School of MedicineShanghai 200092China Department of Biomedical Engineering Columbia UniversityNew YorkNY 10027USA Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science&TechnologyWuhan 430022China

Anti virulence strategy has been considered as one of the most promising approaches to combat drug-rcesistant bacterial ***-forming toxins(PFTs)are the largest class of bacterial toxins,inficting their virulence ffect through creating pores on the cell ***,curent solutions for eliminating PFTs are mostly designed based on their molecular structure,requiring customized design for different *** the present study,we employed erythroliposome(denoted as RM-PL),a biomimetic platform constructed by artificial lipid membranes and natural erythrocyle membranes,to necutralize different hemolytic PFTs regardless of their molecular *** tested with model PFTs,including a-hemolysin,listeriolysin O,and streptolysin O,RM-PL could completely inhibit toxin-induced hemolysis in a concentration-dependent *** vivo studies further confirmed that *** eficiently neuralize various toxins and save animals'lives without causing damage to 0rgans or *** addition,we explored the underlying mechanisms of this efficient detoxification ability and found that it was mainly macrophages in the spleen and the liver that took up RM-PL-absorbed toxins through a variety of endocytosis pathways and digested them in *** summary,the biomimetic RM-PL presented a promising system for broad-spectrum and powerful toxin neutralization with a mech-anism of lysosome-mediaed loxin degradation.

关键词： Eythroliposome Broad-spectrum detoxifcation Hybrid nanovesicle Pore-forming toxins Macrophage uptake Red blood cell membrane Atificial lipid membrane

Dual functions of PsmiR172b-PsTOE3 module in dormancy release and flowering in tree peony (Paeonia suffruticosa)

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Horticulture Research 2023年第4期10卷 174-186页

作者： Yuxi Zhang Linqiang Gao Yanyan Wang Demei Niu Yanchao Yuan chunying liu Xinmei Zhan Shupeng Gai College of Life Sciences Qingdao Agricultural UniversityQingdao266109China University Key Laboratory of Plant Biotechnology in Shandong Province Qingdao266109China

MicroRNAs(miRNAs)are non-coding RNAs that interact with target genes and are involved in many physiological processes in ***172-AP2 mainly plays a role in the regulation of f lowering time and floral organ *** dormancy release is necessary for forcing culture of tree peony in winter,but the mechanism of dormancy regulation is *** this study,we found that a miR172 family member,PsmiR172b,was downregulated during chilling-induced bud dormancy release in tree peony,exhibiting a trend opposite to that of *** ligase-mediated(RLM)5-RACE(rapid amplification of cDNA ends)confirmed that miR172b targeted PsTOE3,and the cleavage site was between bases 12(T)and 13(C)within the complementary site to *** functions of miR172b and PsTOE3 were detected by virus-induced gene silencing(VIGS)and their overexpression in tree peony ***172b negatively regulated bud dormancy release,but PsTOE3 promoted bud dormancy release,and the genes associated with bud dormancy release,including PsEBB1,PsEBB3,PsCYCD,and PsBG6,were *** analysis indicated that PsTOE3 directly regulated PsEBB1 by binding to its promoter,and the specific binding site was a C-repeat(ACCGAC).Ectopic expression in Arabidopsis revealed that the PsmiR172b-PsTOE3 module displayed conservative function in regulating f *** conclusion,our results provided a novel insight into the functions of PsmiR172-PsTOE3 and possible molecular mechanism underlying bud dormancy release in tree peony.

关键词： release functions bases

Nanoplateletsomes for rapid hemostasis performance

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Chinese Chemical Letters 2022年第6期33卷 2937-2941页

作者： Honglan Wang Yuefei Zhu Longlong Zhang Huiwen liu chunying liu Bo Zhang Yanan Song Yu Hu Zhiqing Pang Key Laboratory of Smart Drug Delivery Ministry of EducationSchool of PharmacyFudan UniversityShanghai 201203China Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science&TechnologyWuhan 430022China Department of Biomedical Engineering Columbia UniversityNew YorkNY 10027United States

Uncontrollable hemorrhage remains staple trouble in surgical procedures and a leading cause after major *** bleeding issue may trigger various pathologic scenarios that can lead to tissue morbidities and mortalities,and currently available on-site hemostatic agents are confined to a narrow therapeutic index and may carry the risk of *** by the crucial role of platelets in the process of thrombus,a platelet-mimetic plateletsome with wound targeting and blood coagulation properties is developed for hemorrhage *** is formulated by integrating platelet membranes with functionalized synthetic liposomes and exhibits superior wound targeting and effective hemostasis *** presents less blood loss and shorter hemostasis time than the platelet membrane vehicles or the conventional liposomes in the mouse tail transection *** strong homing of the biomimetic plateletsome to the thrombus was also confirmed,demonstrating the potential of this engineered cell membrane vesicle as a biomimetic hemostat for bleeding treatment.

关键词： Hemostasis Plateletsome Biomimetic Hemorrhage Thrombus targeting

Seasonal variations of dimethylsulfide (DMS) and dim-ethylsulfoniopropionate (DMSP) in the sea-surface microlayer and subsurface water of Jiaozhou Bay and its adjacent area

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Acta Oceanologica Sinica 2009年第2期28卷 73-86页

作者： ZHANG Honghai YANG Guipeng liu chunying LI Chenxuan Key Laboratory of Marine Chemistry Theory and Technology of Ministry of Education College of Chemistry and Chemical Engineering Ocean University of China Qingdao 266100 China

The distributions of DMS and its precursor dimethylsulfoniopropionate, in both dissolved （DMSPd） and particulate fractions （DMSPp） were determined in the sea-surface microlayer and corresponding subsurface water of the Jiaozhou Bay, China and its adjacent area in May and August 2006. The concentrations of all these components showed a clear seasonal variation, with higher concen- trations occurring in summer. This can be mainly attributed to the higher phytoplankton biomass observed in summer. Simultaneously, the enrichment extents of DMSPd and DMSPp in the mi- crolayer also exhibited seasonal changes, with higher values in spring and lower ones in summer. Higher water temperature and stronger radiant intensity in summer can enhance their solubility and photochemical reaction in the microlayer water, reducing their enrichment factors （the ratio of concentration in the microlayer to that in the corresponding subsurface water）. A statistically significant relationship was found between the microlayer and subsurface water concentrations of DMS, DMSP and chlorophyll a, demonstrating that the biogenic materials in the microlayer come primarily from the underlying water. Moreover, our data show that the concentrations of DMSPp and DMS were significantly correlated with the levels of chlorophyll a, indicating that phytoplankton biomass might play an important role in controlling the distributions of biogenic sulfurs in the study area. The ratios of DMS/chlorophyll a and DMSPpchlorophyll a varied little from spring to summer, suggesting that there was no obvious change in the proportion of DMSP producers in the phytoplankton community. The mean sea-to-air flux of DMS from the study area was estimated to be 5.70 μmol/（m^2·d）, which highlights the effects of human impacts on DMS emission.

关键词： Dimethylsulfide (DMS) dimethylsulfoniopropionate (DMSP) sea-surface microlayer flux Jiaozhou Bay

Shenmai injection enhances cisplatin-induced apoptosis through regulation of Mfn2-dependent mitochondrial dynamics in lung adenocarcinoma A549/DDP cells

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Cancer Drug Resistance 2021年第4期4卷 1047-1060页

作者： Yushi Chen Ye Sun Qiuyu Zhao chunying liu Chun Wang College of Integrated Chinese and Western Medical Liaoning University of Traditional Chinese MedicineShenyang 110847LiaoningChina Basic Medical College Shenyang Medical CollegeShenyang 110034LiaoningChina Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications Liaoning University of Traditional Chinese MedicineShenyang 110847LiaoningChina

Aim:Chemoresistance is the biggest obstacle in cancer *** previous study demonstrated that Shenmai injection(SMI),a Chinese herbal medicine,enhanced the antitumor effect of cisplatin via glucose metabolism *** study aimed to further determine whether the SMI sensitizes the non-small cell lung cancer(NSCLC)cells to cisplatin through regulation mitochondrial ***:The Kaplan-Meier Plotter database was used to investigate the relationship between mRNA expression of mitofusin-2(Mfn2)and the survival analysis of NSCLC *** protein expression of Mfn2 in a lung adenocarcinoma tissue chip was detected by immunohistochemistry *** expression of Mfn2 and ATAD3A were compared between cisplatin-sensitive A549 and cisplatin-resistant A549/DDP ***,A549/DDP cells were co-treated with cisplatin and SMI to detect mitochondrial morphology by fluorescent staining,apoptosis-related protein expression with Western blotting,and mitochondrial membrane potential(ΔΨm)with flow cytometry ***:The mean survival time of the Mfn2^(low) group was significantly lower than that of the Mfn2^(high) group by Kaplan-Meier Plotter database analysis,and the Mfn2 protein expression level was lower in cancer tissues than in adjacent *** combination of SMI and cisplatin induced dynamic changes in A549/DDP cells,with increased mitochondrial fusion followed by upregulation of Mfn2 and downregulation of ATAD3A and reduced mitochondrial mass and ΔΨ***,SMI significantly enhanced cisplatin-induced A549/DDP apoptosis,upregulated Bax and the active subunit of caspase-3,and downregulated Bcl-2 expression,as shown via Hoechst staining and flow cytometry ***:Our findings suggest SMI enhances cisplatin-induced apoptosis through regulation of Mfn2-dependent mitochondrial dynamics in cisplatin-resistant lung adenocarcinoma cells.

关键词： SMI NSCLC cisplatin-resistant Mfn2 mitochondrial dynamics apoptosis

A Multilayer Study of pCO2 in the Surface Waters of the Yellow and South China Seas in Spring and the Sea-Air Carbon Dioxide Flux

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Journal of Ocean University of China 2008年第3期7卷 263-268页

作者： liu chunying ZHANG Chuang YANG Xiaoman GONG Haidong ZHANG Zhengbin Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education College of Chemistryand Chemical Engineering Ocean University of China Qingdao 266003 P. R. China

A multilayer study of pCO2 for the Yellow and South China Seas in the surface waters was conducted based on data from four cruises sponsored by the China SOLAS Project in 2005 and 2006,including data for the surface microlayer(SML) ,subsurface layer(SSL) and surface layer(SL) . The carbon fluxes across the air-sea interface were calculated. The results showed that the pCO2 values in the surface waters of the study area decreased in the following order:pCO2 SML> pCO2 SSL> pCO2 SL. The highest values were found in March for all SML,SSL and SL,followed by those in April,and the lowest were in May. The pCO2 values had a significant positive correlation with temperature or salinity. While there was no relationship between pCO2 and longitude,there was a significant negative correlation between it and latitude,i.e.,'high latitude low pCO2'. By using four calculation models,the carbon dioxide fluxes(FC O2) in spring in the Yellow and South China Seas,which were found to act as a 'sink' of atmospheric CO2,were preliminarily estimated on the basis of the pCO2 data in the SML to be -7.00×106 t C and -22.35×106 t C,respectively. It is suggested that the FC O2calculated on the basis of pCO2 data in the SML is more reliable than that calculated on the basis of those in the SL.

关键词： the Yellow Sea South China Sea spring pCO2 multilayer study carbon dioxide fluxes ( FCO2 )

Cytokine nanosponges suppressing overactive macrophages and dampening systematic cytokine storm for the treatment of hemophagocytic lymphohistiocytosis

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Bioactive Materials 2023年第3期21卷 531-546页

作者： Honglan Wang Huiwen liu Jia Li chunying liu Hui Chen Junying Li Chunyan Sun Tao Guo Zhiqing Pang Bo Zhang Yu Hu Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science&TechnologyWuhanHubei430022PR China School of Pharmacy Fudan UniversityKey Laboratory of Smart Drug DeliveryMinistry of Education826 Zhangheng RoadShanghai201203PR China Department of Biomedical Sciences Faculty of MedicineHealth and Human SciencesMacquarie UniversitySydney NSW2109Australia Department of Infectious Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022PR China

Hemophagocytic lymphohistiocytosis(HLH)is a highly fatal condition with the positive feedback loop between continued immune cell activation and cytokine storm as the core mechanism to mediate multiple organ *** by macrophage membranes harbor the receptors with special high affinity for proin-flammation cytokines,lipopolysaccharide(LPS)-stimulated macrophage membrane-coated nanoparticles(LMNP)were developed to show strong sponge ability to both IFN-γand IL-6 and suppressed overactivation of macrophages by inhibiting JAK/STAT signaling pathway both in vitro and in ***,LMNP also efficiently alleviated HLH-related symptoms including cytopenia,hepatosplenomegaly and hepatorenal dysfunction and save the life of mouse ***,its sponge effect also worked well for five human HLH samples in ***,it’s firstly demonstrated that biocompatible LMNP could dampen HLH with high potential for clinical transformation,which also provided alternative insights for the treatment of other cytokine storm-mediated pathologic conditions such as COVID-19 infection and cytokine releasing syndrome during CAR-T therapy.

关键词： Hemophagocytic lymphohistiocytosis Cytokine storm Nanosponge Macrophage activation Macrophage membranes coated nanoparticles Lipopolysaccharide JAK/STAT pathway

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视觉设计中的文化元素融合与多样化表达探索

鞋类工艺与设计 2025年第3期5卷 20-22页

作者：刘春影海南职业技术学院海南海口570000

加强视觉设计中的文化元素融合,有利于加快文化传播速度、满足社会审美需求。然而,在我国视觉设计的文化元素融合现状中,仍旧存在融合重视度不足、表达创新性欠缺、表达方式相对单一等问题。基于此,我国设计人员应运用色彩文化元素,增... 详细信息

加强视觉设计中的文化元素融合,有利于加快文化传播速度、满足社会审美需求。然而,在我国视觉设计的文化元素融合现状中,仍旧存在融合重视度不足、表达创新性欠缺、表达方式相对单一等问题。基于此,我国设计人员应运用色彩文化元素,增强视觉空间表达;应用故事文化元素,巧妙连接视觉设计;应用纹样文化元素,创新文创视觉设计。

关键词：视觉设计文化元素融合多样化表达策略探究

Treatment of Sjogren's Syndrome with Liver Depression

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Journal of Clinical and Nursing Research 2021年第1期5卷 54-56页

作者： Jiaqin liu chunying liu Shaanxi University of Traditional Chinese Medicine China Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine China

liu chunying is a famous old Chinese medicine doctor for more than 30 *** has rich experience in the understanding and treatment of Sjogren's syndrome(SS).liu Shi systematically discusses the etiology and pathogenesis of SS from the angle of liver *** the treatment,it is emphasized that"Muyu Da Zhi"is the first,with the addition and subtraction of the disease,to provide a new idea for the treatment of Sjogren's syndrome from liver depression,and to attach a test case.

关键词： Sjogren's syndrome Liver depression caused dryness Syndrome differentiation Muyu Da Zhi liu chunying Famous medical experience