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氧化应激对公鸡睾丸组织结构及睾酮合成相关基因表达的影响

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农业生物技术学报 2024年第3期32卷 605-616页

作者：吴冰燕曹原刘思丽刘亚美丁香凌义强胡威方满新刘犇王小月宜春学院生命科学与资源环境学院江西绿科农牧科技有限公司江西省高等学校硒农业工程技术研究中心

禽类生长繁殖过程中易受各种应激因素的影响，其中以氧化应激最为突出。为探究氧化应激对鸡(Gallus gallus)睾丸组织结构及睾酮合成相关基因表达的影响，本研究利用敌草快(diquat)作为氧化应激诱导剂，将60只180日龄的健康康乐黄公鸡随... 详细信息

禽类生长繁殖过程中易受各种应激因素的影响，其中以氧化应激最为突出。为探究氧化应激对鸡(Gallus gallus)睾丸组织结构及睾酮合成相关基因表达的影响，本研究利用敌草快(diquat)作为氧化应激诱导剂，将60只180日龄的健康康乐黄公鸡随机平均分成4组：对照组、敌草快5 mg/kg组、敌草快15 mg/kg组和敌草快20 mg/kg组。待21 d处理结束后采集鸡睾丸组织样品，利用q RT-PCR技术和抗氧化功能检测试剂盒分别检测氧化应激相关基因核因子红细胞2相关因子2 (nuclear factor erythroid 2-related factor 2, Nrf2)、半氧合酶1 (hemeoxygenase-1, HO-1)、谷胱甘肽过氧化物酶1 (glutathione peroxidase 1,Gpx1)的表达水平和过氧化氢酶(catalase, CAT)、总超氧化物歧化酶(total superoxide dismutase, T-SOD)的活性及丙二醛(malondialdehyde, MDA)的浓度，确定氧化应激发生情况；利用苏木精-伊红染色法(hematoxylin-eosin staining, HE staining)检测氧化应激对睾丸组织结构的影响，利用Western blot技术分别检测细胞凋亡相关蛋白B细胞淋巴瘤-2 (B-cell lymphoma-2, Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein, Bax)和半胱氨酸蛋白酶3 (Caspase3)的表达量变化，通过qRT-PCR和Western blot技术分别检测睾酮合成相关基因类固醇激素合成急性调节蛋白(steroidogenic acute regulatory protein, StAR)、细胞色素P450胆固醇侧链裂解酶(cytochrome P450 family 11 subfamily A member 1, Cyp11a1)和3β-羟基类固醇脱氢酶1 (3β-hydroxysteroid dehydrogenase 1, Hsd3b1)的mRNA和蛋白表达量变化。结果显示，与对照组相比，不同剂量敌草快组睾丸中氧化应激相关分子HO-1、Nrf2和Gpx1的mRNA水平显著下调(P<0.05),CAT和T-SOD酶活性显著降低(P<0.05),MDA浓度显著升高(P<0.05);HE染色结果表明，睾丸经敌草快诱导作用后结构受到损伤，曲精小管中的精子发生受到明显抑制，随着敌草快处理浓度的升高，曲精小管中的生殖细胞发生严重凋亡；Bax和Caspase3的蛋白表达量显著上调(P<0.05),Bcl-2显著下调(P<0.05)；睾酮合成相关基因StAR、Cyp11a1和Hsd3b1的mRNA及蛋白表达量均发生显著下调(P<0.05)。以上结果表明，氧化应激对鸡睾丸组织结构造成一定程度的损伤，促进睾丸生殖细胞的凋亡，对睾酮合成相关基因的表达具有显著抑制作用。本研究可为探讨氧化应激损伤鸡繁殖性能的机制提供基础资料。

关键词：氧化应激公鸡睾丸细胞凋亡睾酮合成相关基因

Photothermal Effect-based Cytotoxic Ability of Melanin from Mytilus edulis Shells to Heal Wounds Infected with Drug-resistant Bacteria in vivo

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Biomedical and Environmental Sciences 2020年第7期33卷 471-483页

作者： liu ya mei MA Wei Shuai WEI Yu Xi XU Yuan Hong Institute of Biomedical Engineering College of Life SciencesQingdao University\Qingdao 266071ShandongChina Department of Urology Key Laboratory'of Urinary System DiseasesThe A ffiliated Hospital of Qingdao UniversityQingdao 266003ShandongChina

Objective Owing to antibiotic abuse and the subsequent development of antibiotic resistance,bacterial infection has become one of the most persistent unresolved *** antibacterial agents,especially those that are environmental-friendly,are urgently *** Melanin extracted by filtration centrifugation and acid and proteolytic hydrolysis was characterized using UV,FTIR,TEM,and *** conversion was calculated,and the bacteriostatic effects,in vitro and in vivo,were assessed by plate counting and ratios(%)of wound *** Natural melanin hydrolyzed by trypsin had good photothermal conversion effects,which resulted in superior bacteriostatic *** extracted melanin along with laser NIR irradiation at808 nm promoted the healing of wounds infected by drug-resistant bacteria in vivo and was biocompatible according to toxicity tests in vivo and in *** The present findings indicated a safe and efficient method of developing natural antibacterial agents.

关键词： Melanin Photothermal conversion Antibacterial Wound healing

补肾活血汤促进骨质疏松性骨折愈合的主要活性成分及网络药理机制研究

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广州中医药大学学报 2024年第7期41卷 1845-1850页

作者：刘盛海王梓合王梦威王斌刘亚梅广州中医药大学第三临床医学院广东广州510405 广州中医药大学第三附属医院广东广州510378 广州中医药大学中医诊断学教研室广东广州510405

【目的】利用网络药理学方法探讨补肾活血汤治疗骨质疏松性骨折的主要活性成分及药理作用机制。【方法】从中药系统药理学数据库与分析平台(TCMSP)中收集补肾活血汤的化学成分。通过DisGeNET数据库收集骨质疏松症的疾病靶点。通过韦恩... 详细信息

【目的】利用网络药理学方法探讨补肾活血汤治疗骨质疏松性骨折的主要活性成分及药理作用机制。【方法】从中药系统药理学数据库与分析平台(TCMSP)中收集补肾活血汤的化学成分。通过DisGeNET数据库收集骨质疏松症的疾病靶点。通过韦恩图筛选出补肾活血汤治疗骨质疏松症的预测作用靶点。通过基因本体论(GO)功能和京都基因与基因组百科全书(KEGG)通路富集分析获得补肾活血汤治疗骨质疏松性骨折的生物过程和信号通路。采用Cytoscape 3.9.1软件构建“中药成分-疾病靶点-通路”网络图,并通过拓扑参数分析,以度值作为考察,获得补肾活血汤治疗骨质疏松性骨折的主要活性成分。通过在线String 11.5软件构建蛋白-蛋白相互作用(PPI)网络,并通过拓扑参数分析补肾活血汤治疗骨质疏松性骨折在蛋白分子水平上的药理机制。【结果】共筛选出1 236个化学成分,其中包含了72个预测作用靶点,涉及DNA的转录、翻译甚至酶与酶之间的结合等生物过程,与糖尿病、动脉粥样硬化、癌症等KEGG信号通路有交集。【结论】补肾活血汤主要是通过脱氢二甘醇4、yangambin、阿托品、花生四烯酸、鞣花酸、豆甾醇、异鼠李素等主要成分调控核转录因子p65、丝裂原活化蛋白激酶、雌激素受体、肿瘤坏死因子、细胞肿瘤抗原等蛋白分子发挥促进骨质疏松性骨折愈合的作用。

关键词：补肾活血汤骨质疏松骨折网络药理学

Isorhamnetin Downregulates MMP2 and MMP9 to Inhibit Development of Rheumatoid Arthritis through SRC/ERK/CREB Pathway

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Chinese Journal of Integrative Medicine 2024年第4期30卷 299-310页

作者： liu Xiao-rong LI Shuo-fu mei Wen-ya liu Xiang-dan ZHOU Ri-bao College of Pharmacy Hunan University of Chinese MedicineChangsha(410208)China Department of Orthopaedics The First Hospital of Hunan University of Chinese MedicineChangsha(410007)China

Objective To investigate the effect of isorhamnetin on the pathology of rheumatoid arthritis(RA).Methods Tumor necrosis factor(TNF)-α-induced fibroblast-like synoviocytes(FLS)was exposed to additional isorhamnetin(10,20 and 40µmol/L).Overexpression vectors for matrix metalloproteinase-2(MMP2)or MMP9 or SRC were transfected to explore their roles in isorhamnetin-mediated RA-FLS ***-FLS viability,migration,and invasion were ***,a collagen-induced arthritis(CIA)rat model was *** were randomly divided to sham,CIA,low-,medium-,and high-dosage groups using a random number table(n=5 in each group)and administed with normal saline or additional isorhamnetin[2,10,and 20 mg/(kg·day)]for 4 weeks,*** index was calculated and synovial tissue inflammation was determined in CIA *** levels of MMP2,MMP9,TNF-α,interleukin-6(IL-6),and IL-1β,as well as the phosphorylation levels of SRC,extracellular regulated kinase(ERK),and cyclic adenosine monophosphate response element-binding(CREB),were detected in RA-FLS and synovial *** docking was also used to analyze the binding of isorhamnetin to *** In in vitro studies,isorhamnetin inhibited RA-FLS viability,migration and invasion(P<0.05).Isorhamnetin downregulated the levels of MMP2,MMP9,TNF-α,IL-6,and IL-1βin RA-FLS(P<0.05).The overexpression of either MMP2 or MMP9 reversed isorhamnetin-inhibited RA-FLS migration and invasion,as well as the levels of TNF-α,IL-6,and IL-1β(P<0.05).Furthermore,isorhamnetin bound to SRC and reduced the phosphorylation of SRC,ERK,and CREB(P<0.05).SRC overexpression reversed the inhibitory effect of isorhamnetin on RA-FLS viability,migration and invasion,as well as the negative regulation of MMP2 and MMP9(P<0.05).In in vivo studies,isorhamnetin decreased arthritis index scores(P<0.05)and alleviated synovial *** reduced the levels of MMP2,MMP9,TNF-α,IL-6,and IL-1β,as well as the phosphorylation of SRC,ERK,and CRE

关键词： rheumatoid arthritis isorhamnetin SRC/ERK/CREB matrix metalloproteinase

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基于相邻像素统计一致性的非均匀性校正方法

光子学报 2018年第7期47卷 30-38页

作者：刘亚梅长春工业大学机电工程学院长春130012

为了校正长波红外探测器辐射响应非均匀性,提出了一种基于相邻像素统计一致性的非均匀性校正方法.首先,对探测器辐射响应非均匀性建模;然后,利用均值算子估计相邻像素之间的比值,并递推求解校正系数;最后,搭建了原理样机对多种不同场景... 详细信息

为了校正长波红外探测器辐射响应非均匀性,提出了一种基于相邻像素统计一致性的非均匀性校正方法.首先,对探测器辐射响应非均匀性建模;然后,利用均值算子估计相邻像素之间的比值,并递推求解校正系数;最后,搭建了原理样机对多种不同场景成像.实验结果表明:与基于黑体的标定方法相比,利用本文方法校正后的图像粗糙度由1.27×10^(-2)降低至1.13×10^(-2),局部标准差峰值由10.5降低至3.5,可以有效降低红外探测器非均匀性噪声.

关键词：光电探测非均匀性校正相邻像素统计特性红外焦平面阵列相对辐射定标

FPR1 Antagonist (BOC-MLF) Inhibits Amniotic Epithelial-mesenchymal Transition

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Current Medical Science 2024年第1期44卷 187-194页

作者： Xiao-mei HUANG E LIAO Jun-qun LIAO ya-ling liu Yong SHAO Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of Obstetrics Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070China Medical Laboratory Science The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of Obstetrics Yubei Maternity and Child Healthcare HospitalChongqing 400042China

Objective:Premature rupture of membranes(PROM)is a common pregnancy disorder that is closely associated with structural weakening of fetal *** have found that formyl peptide receptor 1(FPR1)activates inflammatory pathways and amniotic epithelial-mesenchymal transition(EMT),stimulates collagen degradation,and leads to membrane weakening and membrane *** purpose of this study was to investigate the anti-inflammatory and EMT inhibitory effects of FPR1 antagonist(BOC-MLF)to provide a basis for clinical prevention of ***:The relationship between PROM,FPR1,and EMT was analyzed in human fetal membrane tissue and plasma samples using Western blotting,PCR,Masson staining,and ELISA ***(LPS)was used to establish a fetal membrane inflammation model in pregnant rats,and BOC-MLF was used to treat the LPS rat *** detected interleukin(IL)-6 in blood from the rat hearts to determine whether the inflammatory model was successful and whether the anti-inflammatory treatment was *** used electron microscopy to analyze the structure and collagen expression of rat fetal ***:Western blotting,PCR and Masson staining indicated that the expression of FPR1 was significantly increased,the expression of collagen was decreased,and EMT appeared in *** rat model indicated that LPS caused the collapse of fetal membrane epithelial cells,increased intercellular gaps,and decreased ***-MLF promoted an increase in fetal membrane collagen,inhibited EMT,and reduced the weakening of fetal ***:The expression of FPR1 in the fetal membrane of PROM was significantly increased,and EMT of the amniotic membrane was ***-MLF can treat inflammation and inhibit amniotic EMT.

关键词： formyl peptide receptor 1 BOC-MLF epithelial-mesenchymal transition premature rupture of membranes

Influence of sea-land breeze on the formation and dissipation of severe dense fog and its burst reinforcement in the Yellow Sea coastal area,China

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Science China Earth Sciences 2024年第2期67卷 432-449页

作者： ya GAO Duanyang liu Shuqi yaN Wenjun ZHOU Hongbin WANG Fan ZU Qin mei Chuanxiang YI Ye SHENG Key Laboratory of Transportation Meteorology of China Meteorological Administration Nanjing210041China yancheng Meteorological Bureau Yancheng224000China Nanjing Joint Institute for Atmospheric Sciences Nanjing210041China Key Laboratory of Disaster Weather Chinese Academy of Meteorological SciencesBeijing100086China

Based on the global reanalysis data of the National Centers for Environmental Prediction(NCEP)/National Center for Atmospheric Research,the surface meteorological observation data,sounding data and satellite observation data,this paper comprehensively analyzes the evolution process and formation mechanism of a persistent severe dense fog process occurred on February 15–17,2015 in yancheng,eastern *** the numerical simulation experiment of Weather Research and Forecast(WRF)model,we further analyze the impact of sea-land breeze on the formation and burst reinforcement of *** show that the precipitation caused by the southwesterly airflow in front of the upper-level trough and the low-pressure inverted trough are conducive to the formation of early rain fog,while the nighttime clear radiance under the control of surface cold high and the infiltration of weak cold advection are conducive to the formation and development of later radiation-advection *** WRF model simulates the fog evolution process,which is basically consistent with the actual fog area,and the simulation results are credible to a certain *** simulation results show that the establishment of sea breeze has an advection cooling effect on the near surface layer,which is conducive to the formation and development of the inversion layer on the near surface,providing stable stratification conditions for the formation and burst reinforcement of *** one hand,the strengthening of sea breeze circulation can continuously transport water vapor to the study *** the other,the occurrence of ultra-low level jet is favorable for the accumulation of low-level water *** the same time,the inversion intensity further strengthens,which is in favor of the burst reinforcement and long-term maintenance of fog.

关键词： Radiation-advection fog Sea-land breeze Atmospheric boundary layer Fog burst reinforcement WRF model

Citrinsorbicillin A,a novel homotrimeric sorbicillinoid isolated by LC-MS-guided with cytotoxic activity from the fungus Trichoderma citrinoviride HT-9

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Chinese Chemical Letters 2024年第8期35卷 299-303页

作者： Guo-Ping Yin ya-Juan Li Li Zhang Ling-Gao Zeng Xue-mei liu Chang-Hua Hu Engineering Research Center of Coptis Development and Utilization(Ministry of Education) College of Pharmaceutical Sciences and Chinese MedicineSouthwest UniversityChongqing 400715China State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 211198China NMPA Key Laboratory for Quality Monitoring of Narcotic Drugs and Psychotropic Substances Chongqing Institute for Food and Drug ControlChongqing 401121China

Citrinsorbicillin A(1),a novel homotrimeric sorbicillinoid,along with two new monomers citrinsor-bicillins B(2)and C(3),were isolated from the Coptis chinensis endophyte Trichoderma citrinoviride HT-9 by liquid chromatograph mass spectrometer(LC-MS)-guided strategy.1 was the first trimeric-example from terrestrial fungi,which possessed a unique carbon skeleton with two bicyclo[2.2.2]octanedione ring connected through an enolated carbon forming by[4+2]*** structures were elucidated by spectroscopic analysis and X-ray diffraction.1 exhibited moderate cytotoxicity against human colon cancer HT29 cells,and it induced significant cell cycle arrest by reducing the protein expression of cyclin D1.

关键词： Citrinsorbicillin A Trimeric sorbicillinoid Coptis chinensis endophyte Trichoderma citrinoviride [4+2]Cycloaddition Cytotoxic activity

Study on carbon emission calculation method and carbon reduction indexes of urban building stock

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Science China(Technological Sciences) 2024年第9期67卷 2706-2726页

作者： yaNG liu ZHANG Nan LU mei LUO ZhiXing DOU mei State Key Laboratory of Green Building Xi’an University of Architecture and TechnologyXi’an 710055China School of Architecture Xi’an University of Architecture and TechnologyXi’an 710055China School of Language Literature and LawXi’an University of Architecture and TechnologyXi’an 710055China School of Management Xi’an University of Architecture and TechnologyXi’an 710055China China Southwest Architectural Design and Research Institute Corp.Ltd. Chengdu 610041China

Carbon emissions associated with buildings are a major source of urban emissions. To put forward the methods and strategies to curb carbon emissions from urban building stock, it is not only necessary to establish a carbon emission calculation method for fine statistical analysis, but also to evaluate carbon emissions of urban planning schemes with applicable indexes. Currently,researches mainly focus on carbon emissions of individual buildings. When expanded to urban building stock, the calculation faces the lack of basic data, inadequate spatial analysis and unspecific carbon reduction indexes. Therefore, this study proposes a bottom-up calculation method for urban building stock, conducts spatial analysis based on carbon balance of urban grids, reveals the coupling mechanism between urban carbon reduction indexes and grid carbon emissions, and systematically establishes a carbon-reduction-oriented urban planning method that comprises calculation, analysis and evaluation, which is applied to Xi'an,China. This study provides a theoretical reference for cities to formulate carbon reduction targets and implement planning strategies by evaluating and predicting carbon emissions from urban building stock.

关键词： carbon emissions urban building stock calculation method carbon reduction index