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Effects of cultivation strategies on the cultivation of Chlorella sp. HQ in photoreactors

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Frontiers of Environmental Science & Engineering 2019年第5期13卷 151-161页

作者： xiaoya liu Yu Hong Peirui liu Jingjing Zhan Ran Yan Beijing Key Laboratory for Source Control Technology of Water Pollution College of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China

The effects of cultivation strategies (including autotrophic cultivation (AC), heterotrophic cultivation (HC), fed-batch cultivation (FC), heterotrophic+ autotrophic two-stage cultivation (HATC), and heterotrophic+ mixotrophic two-stage cultivation (HMTC)) on the growth and lipid accumulation of Chlorella sp. HQ and its total nitrogen (TN) and total phosphorus (TP) removal in secondary effluent were investigated in column photoreactors. The results showed that the TN and TP removal rates ranged between 93.72%–95.82% and 92.73%–100%, respectively, under the five different strategies. The microalgal growth potential evaluated by the maximal growth rate (Rmax) was in the order of HMTC>HC>FC>AC>HATC. The values of biomass, total lipid yield, triacylglycerols (TAGs) yield, and total lipid content of the microalga cultivated in the last 5 d increased significantly, but the TAGs productivities of the five strategies were lower than those in the first 7 d. Compared with all the other cultivation strategies, the TAGs productivity and yield after 12 d of cultivation under the heterotrophic condition reached the highest values accompanying the highest level of intracellular reactive oxygen species (ROS), in which the TAGs yield reached 40.81 mg/L at the end of the cultivation period. The peaks in TAGs yield and ROS level suggested that HC was beneficial for lipids accumulation via regulating the cellular redox status and exerting ROS stress on microalgal cells. In summary, HMTC was the best cultivation strategy for improving the microalgal biomass and HC was the best strategy for microalgal TAGs accumulation to produce biodiesel.

关键词： Chlorella sp.HQ Cultivation strategy lipids Nitrogen removal Phosphorus removal Reactive oxygen species

Pore-water distribution and quantification of diffusive benthic fluxes of nutrients in the Huanghai and East China Seas sediments

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Acta Oceanologica Sinica 2006年第1期25卷 90-99页

作者： NI Jianyu liu xiaoya CHEN Qijiang LIN Yian Key Laboratory of Submarine Geosciences of State Oceanic Administration Hangzhou 310012 China Second Institute of Oceanography State Oceanic Administration Hangzhou 310012 China Laboratory of Marine Ecosystem and Biogeochemistry of State Oceanic Administration Hangzhou 310012 China

The distribution of nitrate, nitrite, ammonia, phosphate and silicate in pore-water and their exchange between sediments and overlying waters （benthic flux） were determined at nine locations on the shelve of Huanghai and East China Seas. On the basis of the redox potential of sediments and nutrients distributions in the pore-waters, it is found that the benthic sediments are being in a suboxic to anoxic environment in the Huanghai and East China Seas. The nutrients distribution in the pore-waters is mainly controlled by the sediment environment, and ammonia is the major inorganic nitrogen in the pore-waters. On the basis of benthic fluxes of nutrients calculated us- ing Fick＇s first law, there is remarkable efflux of ammonia, dissolved inorganic nitrogen（DIN）, phosphate and silicate from the sediments to the overlying waters in the study area, and their benthic fluxes are 299.3-2 214.8, 404.4-2 159.5 , 5.5-18.8 and 541.3-1 781.6 μmol/（m^2·d） respectively, and perhaps they are the major source of dissolved inorganic nitrogen, phosphate and silicate for the overlying water. At most stations, the nitrate flux was from the overlying waters to the sediments, which suggests that suboxic organic matter decomposition via denitrification is dominated in the most area of Huanghai and East China Seas. High benthic fluxes appearing in the coastal area and relatively low benthic fluxes occurring in the shelf area are found and are consistent with pri- mary productivity zoning in the study area. On the other hand, the ammonia flux displays an exponential decrease with water depth increase and an exponential increase with the bottom water temperature. However, others do not display this trend.

关键词： nutrients pore-water benthic flux Huanghai and East China Seas

Preparation and Properties of Aqueous SCNTs Dispersion based on A UV-curable Polymeric Dispersant

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Journal of Wuhan University of Technology(Materials Science) 2018年第2期33卷 485-491页

作者：袁妍 WU Haiqiang liu Jingcheng LUO Jing liu Ren liu xiaoya The Key Laboratory of Synthestic and Biological Colloids Ministry of EducationSchool of Chemical and Material EngineeringJiangnan University

A novel photosensitive copolymer P（SS-co-AA-g-GMA）（PSAG） was synthesized and utilized to noncovalently functionalize pristine single-walled carbon nanotubes（SCNTs）. PSAG was highly effective for the solubilization of SCNTs in water and validated by UV-vis absorption spectra experiments, resulting in homogeneous and stable PSAG-SCNT aqueous dispersion. The microstructure of SCNTs was observed through Raman spectroscopy and transmission electron microscopy. In addition, compared with the two common polymeric dispersants of Triton X-100 and PSS, PSAG demonstrated more effective performances for dispersing SCNTs under identical conditions. Furthermore, the photosensitive PSAG-SCNTs can be crosslinked after UV irradiation, leading to significant improvement in the water resistance of SCNT films. UV-cured films can be transferred to plastic wrap to form a flexible film with high electrical conductivity.

关键词： single-walled carbon nanotubes(SCNTs) polymeric dispersant UV-curable conductive water resistance

Synthesis of submicron-sized composite particles with unique morphology by emulsifier-free seeded emulsion copolymerization

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Particuology 2008年第5期6卷 369-375页

作者： Hong Chu Hongyan Wang Zhongbin Ni xiaoya liu Mingqing Chen School of Chemical and Material Engineering Jiangnan University Wuxi 214122 China

Polystyrene （PSt） microspheres with diameter of 375 nm to be used as the seeds for seeded emulsion polymerization were prepared via emulsion polymerization using potassium persulfate （KPS） as initiator in ethanol-water mixed solvents. Emulsifier-free seeded emulsion copolymerization of styrene （St） with acrylonitrile （AN） was carried out in the presence of poly（ethylene glycol） monomethoxymonomethacrylate （PEGm） macromonomer as reactive stabilizer and 2,2＇-azobisisobutyronitrile （AIBN） as initiator to obtain submicron-sized PEGm graft poly（styrene-coacrylonitrile）（PEGm-g-PSAN） composite particles with unique morphology. Scanning electron microscopy （SEM） indicated that St and AN together contributed to forming the unusual morphology. The concentration of St and AN, total monomer concentration, initiator type and the monomer adding method remarkably affected the morphology of the composite polymer particles.

关键词： Seed Composite particle Emulsifier-free emulsion copolymerization Morphology

Imidazole functionalized photo-crosslinked aliphatic polycarbonate drug-eluting coatings on zinc alloys for osteogenesis,angiogenesis,and bacteriostasis in bone regeneration

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Bioactive Materials 2024年第7期37卷 549-562页

作者： Wei Zhang Miao Dai Ye Zhu Siyuan Li Ying Sun xiaoya liu Xiaojie Li Key laboratory of synthetic and biological Colloids Ministry of EducationSchool of Chemical and Material EngineeringJiangnan UniversityLihu Street 1800Wuxi214122China

Zinc(Zn)alloys have demonstrated significant potential in healing critical-sized bone ***,the clinical application of Zn alloys implants is still hindered by challenges including excessive release of zinc ions(Zn^(2+)),particularly in the early stage of implantation,and absence of bio-functions related to complex bone repair ***,a biodegradable aliphatic polycarbonate drug-eluting coating was fabricated on zinc-lithium(Zn-Li)alloys to inhibit Zn^(2+)release and enhance the osteogenesis,angiogenesis,and bacteriostasis of Zn ***,the photo-curable aliphatic polycarbonates were co-assembled with simvastatin and deposited onto Zn alloys to produce a drug-loaded coating,which was crosslinked by subsequent UV light *** the 60 days long-term immersion test,the coating showed distinguished stable drug release and Zn^(2+)release inhibition *** from the regulated release of Zn^(2+)and simvastatin,the coating facilitated the adhesion,proliferation,and differentiation of MC3T3-E1 cells,as well as the migration and tube formation of ***926 ***,the coating also showed remarkable antibacterial properties against both *** and *** in vivo rabbit critical-size femur bone defects model demonstrated that the drug-eluting coating could efficiently promote new bone formation and the expression of platelet endothelial cell adhesion molecule-1(CD31)and osteocalcin(OCN).The enhancement of osteogenesis,angiogenesis,and bacteriostasis is achieved by precisely controlling of the released Zn^(2+)at an appropriate level,as well as the stable release profile of *** tailored aliphatic polycarbonate drug-eluting coating provides significant potential for clinical applications of Zn alloys implants.

关键词： Zn alloy implant Aliphatic polycarbonate Drug-eluting coating Osteogenesis Angiogenesis

Study on chemical forms of fluorine enrichment of Antarctic krill

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Acta Oceanologica Sinica 2000年第4期19卷 63-71页

作者： Pan Jianming, Zhang Haisheng, liu xiaoya (Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China) Second Institute of Oceanography State Oceanic Administration Hangzhou 310012 China

The different forms fluoine in different parts of molting process of krill was analyzed by culture of Antarctic krill (Euphausia superba). The results shaw that fluorine amount in cuticle is up to 90% of total in the krill. During the krill molting process, the fluorine content in cuticle and muscle varies alternatively. This is to say, the fluorine content in new cuticle and muscle is much lower after molted, the fluorine in krill comes from seawater by biological absorption of the krill, and the fluorine absorbed by krill lost with the old cuticle at the end stage of molting process. Meanwhile, the new molting process starts again. The evidence is proved by the change of different forms fluorine content in cuticle and muscle during krill's molting process. At last the author proposes the fluorine enriching process model of Antarctic krill.

关键词： Antarctic krill fluorine enrichment chemical forms

Development of Stainless Steel Products in Eastern Special Steel

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Development of Stainless Steel Products in Eastern Special S...

Effect of integrated Traditional Chinese and Western Medicine on Gout

第十届中国钢铁年会暨第六届宝钢学术年会

作者： liu xiaoya Zhenshi Group Eastern Special Steel Co. Ltd.

＜正＞Eastern Special Steel Co.,Ltd was established by Zhenshi Holding Group Co.,Ltd after acquiring the 100%ownership of the former Jiaxing Eastern Iron＆Steel Co.,Ltd in July *** company imported 80-ton energy-saving environment-friendly CONSTEEL electric arc furnaces from Italy,possess 90-ton AOD converters,90-ton ladle furnaces,a1600 slab continuous casters,1800 Steckel mills,medium wide plate finishing equipment,annealing and picking equipment.

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Journal of Traditional Chinese Medicine 2021年第5期41卷 806-816页

作者： liu Wei WU Yuanhao XUE Bin JIN Yue ZHANG Shumin LI Peihao XIE Qing WANG Aihua GAO Jingyue CAI Yue ZHANG Bo liu xiaoya WANG Yi DUAN Ran Department of Rheumatology and Immunology First Teaching Hospital of Tianjin University of Traditional Chinese MedicineTianjin 300381China National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion Tianjin 300381China Tianjin Key Laboratory of Translational Research of Traditional Chinese Medicine Prescription and Syndrome Tianjin 300381China

OBJECTIVE:To evaluate the curative effect of integrated Traditional Chinese and Western Medicine on gout,and to investigate the therapy timing and exact treatment options of integrated ***:Totally 860 patients were enrolled,including 460 patients with intermittent gout,200 patients with active Traditional Chinese Medicine(TCM)syndrome(TCM syndrome score≥6)and200 patients with stable TCM syndrome(score<6).They were randomly divided into intervention and control *** control group was treated according to Western Medicine *** intervention group was treated with integrated Traditional Chinese and Western *** efficacy of TCM syndrome,joint pain score,joint swelling score,ESR,C-reactive protein,serum uric acid,liver and kidney function,and the duration of remission of TCM syndrome were compared between the two groups before and after ***:For the patients with stable TCM syndrome,there was no significant difference in the effective rate and inefficiency between the intervention group and the control *** the active type,the effective rate of the intervention group is better than the control group *** the stable type,there was no significant difference between the intervention group and the control group in improving the scores of joint pain and swelling,reducing the level of ESR,C-reactive protein,serum uric acid and improving liver and kidney *** the active type,the differences between the two groups were *** stable stage of gout in the intervention group was longer than the control ***:For the gout patients with stable TCM syndrome in the acute stage of gout,we can use TCM treatment or Western Medicine alternatively;for the patients with active TCM syndrome,the scheme of combination of Traditional Chinese and Western Medicine can be applied,with the better curative effect than any medicine alone.

关键词： gout integrative medicine diagnosis and treatment system curative effect

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A Photosensitive Copolymer for UV-curable Electrodeposition Coatings

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Journal of Wuhan University of Technology(Materials Science) 2011年第6期26卷 1098-1102页

作者：刘仁刘晓亚 School of Chemical and Material Engineering Jiangnan University

A series of photosensitive random copolymers （UPDHES） were prepared by introducing acrylate groups onto the side chain of the copolymer backbone of N, N-domethyl amimethyl methacrylate （DMAEMA）, 2-hydroxypropyl acrylate （HEA）, 2-ethylhexyl acrylate （EHA）, and styrene （St）（PDHES）. The molecular structure of UPDHES was characterized by FTIR, 1HNMR and GPC. The photopolymerization kinetics of UPDHES with different C=C content was investigated using real time FTIR in which it was found that the UPDHES system had notable photosensitivity. The effect of C=C content on the properties of cured films were studied by evaluating various film properties such as thermal stability, glass transition temperature and tensile properties. The thermal degradation of cured films was investigated via thermogravimetric analysis/infrared spectrometry （TGA-IR）. Thus a series of UV-curable electrodeposition coatings with good photosensitivity and mechanical properties were prepared from a low-cost photosensitive random copolymer.

关键词： UV-curable electrodeposition coatings photosensitivity