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Dual-directional regulation of spinal cord injury and the gut microbiota

Neural Regeneration Research 2024年第3期19卷 548-556页

作者： Yinjie Cui Jingyi liu Xiao Lei shuwen liu Haixia Chen Zhijian Wei Hongru Li Yuan Yang Chenguang Zheng Zhongzheng Li Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina School of Medical Technology Tianjin University of Traditional Chinese MedicineTianjinChina Research Center of Experimental Acupuncture Science Tianjin University of Traditional Chinese MedicineTianjinChina International Cooperation and Exchange Office Chengdu University of Traditional Chinese MedicineChengduSichuan ProvinceChina International Cooperation Base of Spinal Cord Injury Tianjin Key Laboratory of Spine and Spinal Cord InjuryDepartment of OrthopedicsTianjin Medical University General HospitalTianjinChina Department of Orthopedics Qilu Hospital of Shandong UniversityJinanShandong ProvinceChina

There is increasing evidence that the gut microbiota affects the incidence and progression of central nervous system diseases via the brain-gut *** spinal cord is a vital important part of the central nervous system;however,the underlying association between spinal cord injury and gut interactions remains *** studies suggest that patients with spinal cord injury frequently experience intestinal dysfunction and gut *** in the gut microbiota can cause disruption in the intestinal barrier and trigger neurogenic inflammatory responses which may impede recovery after spinal cord *** review summarizes existing clinical and basic research on the relationship between the gut microbiota and spinal cord *** research identified three key ***,the gut microbiota in patients with spinal cord injury presents a key characteristic and gut dysbiosis may profoundly influence multiple organs and systems in patients with spinal cord ***,following spinal cord injury,weakened intestinal peristalsis,prolonged intestinal transport time,and immune dysfunction of the intestine caused by abnormal autonomic nerve function,as well as frequent antibiotic treatment,may induce gut ***,the gut microbiota and associated metabolites may act on central neurons and affect recovery after spinal cord injury;cytokines and the Toll-like receptor ligand pathways have been identified as crucial mechanisms in the communication between the gut microbiota and central nervous *** microbiota transplantation,probiotics,dietary interventions,and other therapies have been shown to serve a neuroprotective role in spinal cord injury by modulating the gut *** targeting the gut microbiota or associated metabolites are a promising approach to promote functional recovery and improve the complications of spinal cord injury.

关键词： chemokines cytokines gut microbiota NLRP3 spinal cord injury Toll-like receptor ligand tryptophan

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Engineered extracellular vesicles efficiently deliver CRISPR-Cas9 ribonucleoprotein(RNP)to inhibit herpes simplex virus1 infection in vitro and in vivo

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1362-1379页

作者： Yuanda Wan Liren Li Ruilin Chen Jiajia Han Qiyun Lei Zhipeng Chen Xiaodong Tang Wenyu Wu shuwen liu Xingang Yao NMPA Key Laboratory for Research and Evaluation of Drug Metabolism Guangdong Provincial Key Laboratory of New Drug ScreeningSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Center of Clinical Pharmacy Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060China Key Laboratory of Infectious Diseases Research in South China(Southern Medical University) Ministry of EducationGuangzhou 510515China

Extracellular vesicles(EVs)have recently emerged as a promising delivery platform for CRISPR/Cas9 ribonucleoproteins(RNPs),owing to their ability to minimize off-target effects and immune ***,enhancements are required to boost the efficiency and safety of Cas9 RNP enrichment within *** response,we employed the Fc/Spa interaction system,in which the human Fc domain was fused to the intracellular domain of PTGFRN-Δ687 and anchored to the EV ***,the B domain of the Spa protein was fused to the C domain of cargos such as Cre or *** to the robust interaction between Fc and Spa,this method enriched nearly twice the amount of cargo within the *** loaded with spCas9 RNP targeting the HSV1 genome exhibited significant inhibition of viral replication in vitro and in ***,following neuron-targeting peptide RVG modification,the in vivo dosage in neural tissues substantially increased,contributing to the clearance of the HSV1 virus in neural tissues and exhibiting a lower off-target *** findings establish a robust platform for efficient EV-based SpCas9 delivery,offering potential therapeutic advantages for HSV1 infections and other neurological disorders.

关键词： Extracellular vesicles CRISPR/Cas9 Ribonucleoproteins PTGFRN Fc/Spa HSV1 Neuron-targeting Delivery

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马铃薯三糖甘草次酸衍生物通过抑制SARS-CoV-2进入靶细胞作为潜在的小分子新冠病毒融合抑制剂

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南方医科大学学报 2024年第3期44卷 474-483页

作者：万欣洪崇竣王进绅宋高鹏刘叔文惠州卫生职业技术学院药学与检验学院广东惠州516000 华南农业大学材料与能源学院广东广州510642 南方医科大学药学院广东广州510515

目的研究马铃薯三糖甘草次酸衍生物能否通过抑制SARS-CoV-2进入靶细胞,作为潜在的小分子新冠病毒融合抑制剂。方法以天然SARS-CoV-2进入抑制剂甘草酸为先导化合物,利用活性亚结构的拼合原理等设计并合成了系列马铃薯三糖甘草次酸衍生... 详细信息

目的研究马铃薯三糖甘草次酸衍生物能否通过抑制SARS-CoV-2进入靶细胞,作为潜在的小分子新冠病毒融合抑制剂。方法以天然SARS-CoV-2进入抑制剂甘草酸为先导化合物,利用活性亚结构的拼合原理等设计并合成了系列马铃薯三糖甘草次酸衍生物。利用SARS-CoV-2假病毒体外细胞感染模型,检测该系列甘草次酸衍生物的体外抗SARS-CoV-2活性;利用表面等离子共振技术及假病毒模型寻找先导化合物1b的抗病毒作用靶点;利用SARS-CoV-2 S蛋白介导的细胞-细胞融合体系,检测先导化合物1b是否作用于SARS-CoV-2病毒入侵宿主的膜融合过程;基于分子对接与定点突变技术,确定先导化合物1b与S蛋白的作用模式等。结果先导化合物1b对SARS-CoV-2奥密克戎假病毒有显著抑制作用,EC50值为3.28μmol/L(P<0.05),对其它SARS-CoV-2变异株假病毒有广谱抗病毒活性。细胞-细胞膜融合实验显示1b能够抑制合胞体的形成。分子对接预测先导化合物1b可与S1与S2亚基交界处的空腔中的Glu309、Ser305、Arg765、Lys964等多个保守氨基酸残基产生氢键作用,亲和力为-8.6 kcal/mol。化合物1b在10、5、2.5、1.25μmol/L时对Arg765、Lys964、Glu309和Leu303突变后的假病毒的抑制活性显著降低(P<0.01)。结论马铃薯三糖甘草次酸衍生物能够靶向作用于S蛋白,特异性干扰病毒-细胞膜融合阶段,继而发挥抗SARS-CoV-2感染的作用,是一类结构新颖的小分子SARS-CoV-2融合抑制剂。

关键词： SARS-CoV-2 小分子新冠病毒融合抑制剂刺突蛋白甘草次酸衍生物

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过度维权与敲诈勒索罪边界研究

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安徽冶金科技职业学院学报 2024年第1期34卷 55-56,61页

作者：刘书文庄乾龙北京林业大学人文社会科学学院北京100083

由于实务中案件情况复杂,加之法律本身固有的滞后性,行为人的过度维权行为与敲诈勒索罪之间存在着一条并不明晰的界限。为厘清过度维权与敲诈勒索罪的边界,通过研究相关案例,分析实务中过度维权入刑的司法现状,从知假买假者的身份认定... 详细信息

由于实务中案件情况复杂,加之法律本身固有的滞后性,行为人的过度维权行为与敲诈勒索罪之间存在着一条并不明晰的界限。为厘清过度维权与敲诈勒索罪的边界,通过研究相关案例,分析实务中过度维权入刑的司法现状,从知假买假者的身份认定、维权依据、索赔数额和维权手段几个角度解构司法认定争议,尝试划清两者界限。过度维权行为与敲诈勒索罪的界限认定,应当在法益价值衡量与刑法谦抑性原则的基础上,综合考虑市场秩序现状、公众法治意识、维权成本与权利救济途径,谨慎入罪。

关键词：消费者过度维权敲诈勒索罪

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Characteristics of Hydrocarbon Fluid Inclusions and Their Significance for Evolution of Petroleum Systems in the Dabashan Foreland,Central China

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Acta Geologica Sinica(English Edition) 2015年第3期89卷 861-875页

作者： LI Rongxi QIN Xiaoli DONG shuwen liu shuwen School of Earthscience and Resources Chang'an University Key Laboratory for the study of Focused Magmatism and Giant Ore Deposits MLR Chinese Academy of Geological Sciences

Field investigation combined with detailed petrographic observation indicate that abundant oil,gas,and solid bitumen inclusions were entrapped in veins and cements of sedimentary rocks in the Dabashan foreland,which were used to reconstruct the oil and gas migration history in the context of tectonic *** stages of veins were recognized and related to the collision between the North China block and the Yangtze block during the Indosinian orogeny from Late Triassic to Early Jurassic（Dl）,the southwest thrusting of the Qinling orogenic belt towards the Sichuan basin during the Yanshanian orogeny from Late Jurassic to Early Cretaceous（D2）,and extensional tectonics during Late Cretaceous to Paleogene（D3）,*** occurrences of hydrocarbon inclusions in these veins and their homogenization temperatures suggest that oil was generated in the early stage of tectonic evolution,and gas was generated later,whereas solid bitumen was the result of pyrolysis of previously accumulated *** stages of hydrocarbon fluid inclusions were also identified in cements of carbonates and sandstones of gas beds in the Dabashan foreland belt and the Dabashan foreland depression（northeastern Sichuan basin）,which recorded oil/gas formation,migration,accumulation and destruction of paleo-reservoirs during the *** analysis of hydrocarbon fluid inclusions contained in vein minerals shows that δ^（13）C_1 of gas in fluid inclusions ranges from-17.0‰ to-30.4‰（PDB） and δD from-107.7‰ to-156.7‰（SMOW）,which indicates that the gas captured in the veins was migrated natural gas which may be correlated with gas from the gas-fields in northern Sichuan *** geochemical comparison between bitumen and potential source rocks indicates that the Lower Cambrian black shale and the Lower Permian black limestone were the most possible source rocks of the *** with tectonic evolution history of the Dabashan foreland,the results of this study suggest that oil

关键词： Hydrocarbon fluid inclusion bitumen foreland mineral vein paleo- reservoir Dabashanforeland

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不同果胶酶处理对户太八号葡萄酒品质的影响

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中国酿造 2024年第5期43卷 68-74页

作者：张萃异刘辉张碧颖黄佳骏石侃刘树文西北农林科技大学葡萄酒学院陕西杨陵712100 陕西省葡萄与葡萄酒工程技术研究中心陕西杨凌712100 国家林业和草原局葡萄与葡萄酒工程技术研究中心陕西杨凌712100 西北农林科技大学合阳葡萄试验示范站陕西渭南715300 西北农林科技大学宁夏贺兰山东麓葡萄酒试验站宁夏永宁750104

为探究不同果胶酶种类和添加量对户太八号葡萄酒整体品质的影响,以户太八号为原料酿造干红葡萄酒,研究3种商业果胶酶(HC、EX-V、RF)在不同添加量(20 mg/L、40 mg/L、60 mg/L和80 mg/L)下对户太八号葡萄出汁率、葡萄酒澄清度、理化指标... 详细信息

为探究不同果胶酶种类和添加量对户太八号葡萄酒整体品质的影响,以户太八号为原料酿造干红葡萄酒,研究3种商业果胶酶(HC、EX-V、RF)在不同添加量(20 mg/L、40 mg/L、60 mg/L和80 mg/L)下对户太八号葡萄出汁率、葡萄酒澄清度、理化指标、甲醇含量、颜色和挥发性香气化合物的影响。结果表明,当果胶酶EX-V添加量为20 mg/L时,葡萄酒整体品质较好,葡萄酒的pH值、酒精度、挥发酸等指标无显著性影响(P>0.05),且甲醇含量满足GB/T 15037—2006《葡萄酒》标准要求,同时显著提高了葡萄的出汁率、葡萄酒的澄清度(P0.1)可以区分不同果胶酶处理葡萄酒样。研究表明,添加20 mg/L果胶酶EX-V能显著提高户太八号葡萄酒的品质。

关键词：户太八号葡萄酒果胶酶香气成分颜色出汁率

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酒酒球菌和植物乳杆菌接种时序对苹果酸-乳酸发酵的影响

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食品与发酵工业 2024年第14期50卷 78-87页

作者：刘辉张嘉璇张碧颖李进周鹏辉管雪强石侃刘树文西北农林科技大学葡萄酒学院陕西杨凌712100 山东省葡萄研究院山东济南250100 山东省酿酒葡萄与葡萄酒技术创新中心山东济南250100 陕西省葡萄与葡萄酒工程技术研究中心陕西杨凌712100 国家林业和草原局葡萄与葡萄酒工程技术研究中心陕西杨凌712100 西北农林科技大学合阳葡萄试验示范站陕西渭南715300 西北农林科技大学宁夏贺兰山东麓葡萄酒试验站宁夏永宁750104

随着对酒酒球菌和植物乳杆菌发酵性能的进一步认识,共接种策略在苹果酸-乳酸发酵(malolactic fermentation,MLF)中的探索也逐渐深入。该研究以赤霞珠、蛇龙珠葡萄酒为原料,使用本土酒酒球菌SD2a(OeSD2a)和植物乳杆菌XJ25(LpXJ25)进行共... 详细信息

随着对酒酒球菌和植物乳杆菌发酵性能的进一步认识,共接种策略在苹果酸-乳酸发酵(malolactic fermentation,MLF)中的探索也逐渐深入。该研究以赤霞珠、蛇龙珠葡萄酒为原料,使用本土酒酒球菌SD2a(OeSD2a)和植物乳杆菌XJ25(LpXJ25)进行共接种发酵,通过监测MLF过程中生物量及有机酸的动态变化,分析发酵结束后酒样的香气化合物组成与含量。结果表明,顺序接种能够显著提升苹果酸降解速率、丰富葡萄酒香气物质。各顺序接种发酵组最终的苹果酸降解量均高于同时接种组(蛇龙珠葡萄酒中最高98.21%,赤霞珠葡萄酒中最高99.24%),(0 h)OeSD2a+(24 h)LpXJ25的接种顺序在香气物质合成方面表现最优,发酵结束后获得了最高的香气化合物总量(蛇龙珠葡萄酒中为486.72 mg/L,赤霞珠葡萄酒中为504.24 mg/L),显著提升了壬酸乙酯、月桂酸乙酯、α-萜品醇等的含量,改善了酒样香气表现。因此,共发酵条件下选择适当的接种顺序对提高MLF效率和葡萄酒香气品质提升有着重要意义。

关键词：苹果酸-乳酸发酵酒酒球菌植物乳杆菌共接种葡萄酒香气

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天然气掺氢对站场放空系统的影响规律分析

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石油工程建设 2024年第1期50卷 50-53页

作者：刘权徐红果刘书文中国石油工程建设有限公司四川科宏公司四川成都610000

为了掌握天然气掺氢后对站场放空系统的影响规律,利用ASPEN HYSYS流程模拟软件,分析天然气掺氢后放空系统的适应性。结果表明:天然气掺氢后安全阀额定泄放量始终大于所需泄放量,原安全阀能满足泄放要求;天然气掺氢不会导致放空系统运行... 详细信息

为了掌握天然气掺氢后对站场放空系统的影响规律,利用ASPEN HYSYS流程模拟软件,分析天然气掺氢后放空系统的适应性。结果表明:天然气掺氢后安全阀额定泄放量始终大于所需泄放量,原安全阀能满足泄放要求;天然气掺氢不会导致放空系统运行温度超出设计范围,反而对放空管道的低温工况有明显的抑制作用;天然气掺氢会使安全阀背压轻微减小,不会导致安全阀背压及放空系统运行压力超压;天然气掺氢后放空系统管径均满足泄放要求;随着掺氢比例的提高,放空火炬的辐射热距离逐渐减小。

关键词：天然气掺氢放空系统掺氢比例安全阀马赫数

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Paleoproterozoic Potassic Granitoids in the Sushui Complex from the Zhongtiao Mountains,Northern China:Geochronology,Geochemistry and Petrogenesis

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Acta Geologica Sinica(English Edition) 2006年第6期80卷 875-885页

作者： TIAN Wei liu shuwen ZHANG Huafeng Key Laboratory of Orogenic Belt and Crustal Evolution of the Ministry of Education School of Earth and Space Sciences Peking University Beijing 100871 State Key Laboratory of Geo-Processes and Mineral Resources China University of Geosciences Beijing 100083

Paleoproterozoic potassic granitoids in the southern Sushui Complex from the Zhongtiao Mountains yielded SHRIMP zircon U-Pb ages of 1968-1944 Ma. Lithologically, the potassic granitoid series consists chiefly of monzodiorite, quartz monzonite and syenogranite. Their trace elements and Sm-Nd isotope characteristics indicate that they were derived from partial melting of Archean TTG rocks in an overthickened continental crust. Petrogenesis of this potassic granitoid series implies a collisional environment within the Trans-North China Orogen in the Paleoproterozoic, which supports a tectonic model of Eastern and Western Continental Blocks being amalgamated in the Paleoproterozoic.

关键词： Sushui Complex Zhongtiao Block Trans-North China Orogen potassic granitoid zircon U-Pb Sm-Nd isotope Paleoproterozoic

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Structures of Syn-deformational Granites in the Longquanguan Shear Zone and Their Monazite Electronic Microprobe Dating

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Acta Geologica Sinica(English Edition) 2006年第6期80卷 864-874页

作者： ZHANG Jinjiang ZHAO Lan liu shuwen The Key Laboratory of Orogenic Belts and Crustal Evolution Ministry of EducationSchool of Earth and Space SciencesPeking UniversityBeijing 100871

The Longquanguan shear zone is an important structural belt in the North China Craton, separating the underlying Fuping complex from the overlying Wutai complex. This shear zone has experienced three episodes of deformation: the first and main episode is a ductile top-to-ESE shear along the gently northwest-west dipping foliations, while the other two episodes are later collapse sliding. Prolonged granites parallel to the shear foliations make one of the main compositions of the Longquanguan shear zone. These granites experienced deformation to form mylonitic rocks when they emplaced during the first episode of deformation. Structural characters of the granites and their contacts to the country rocks indicate that these granites possibly resulted from in-situ partial remelting by shearing, i.e., they are syn-deformational granites. Monazites in these mylonitic granites are magmatic minerals and their crystallization ages may represent ages of the magmatic events, and also the ages for the main deformation of the Longquanguan shear zone. Monazite electronic microprobe dating were carried on two samples of granite, which gives multiple peak ages, among which 1,846 Ma and 1,877 Ma are the main peak ages for the two samples. These ages represent the main deformation of the Longquanguan shear zone, which is consistent with the main regional geological event at about 1,850 Ma caused by the collision between the Eastern and Western Blocks in North China. The good match between the monazite ages and the corresponding regional tectono-thermal events shows the feasibility and reliability of monazite electronic microprobe dating.

关键词： Longquanguan shear zone syn-deformational granite monazite electronic microprobe dating

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