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Host-Induced Gene Silencing of Effector AGLIP1 Enhanced Resistance of Rice to Rhizoctonia solani AG1-IA

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Rice science 2024年第4期31卷 463-474,I0031-I0034页

作者： ZHAO mei liu Xiaoxue WAN Jun ZHOU Erxun shu Canwei Pesticide State Key Laboratory of Green Pesticide Guangdong Province Key Laboratory of Microbial Signals and Disease ControlCollege of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China College of Life Science and Engineering Henan University of Urban ConstructionPingdingshan 467036China

Rice sheath blight, caused by Rhizoctonia solani AG1-IA, is a major disease in rice-growing areas worldwide. Effectors of phytopathogenic fungi play important roles during the infection process of fungal pathogens onto their host plants. However, the molecular mechanisms by which R. solani effectors regulate rice immunity are not well understood. Through prediction, 78 candidate effector molecules were identified. Using the tobacco rattle virus-host induced gene silencing(TRV-HIGS) system, 45 RNAi constructs of effector genes were infiltrated into Nicotiana benthamiana leaves. The results revealed that eight of these constructs resulted in a significant reduction in necrosis caused by infection with the AG1-IA strain GD-118. Additionally, stable rice transformants carrying the double-stranded RNA construct for one of the effector genes, AGLIP1, were generated to further verify the function of this gene. The suppression of the AGLIP1 gene increased the resistance of both N. benthamiana and rice against GD-118, and also affected the growth rate of GD-118, indicating that AGLIP1 is a key pathogenic factor. Small RNA sequencing showed that the HIGS vectors were processed into si RNAs within the plants and then translocated to the fungi, leading to the silencing of the target genes. As a result, AGLIP1 might be an excellent candidate for HIGS, thereby enhancing crop resistance against the pathogen and contributing to the control of R. solani infection.

关键词： Rhizoctonia solani host-induced gene silencing lipase tobacco rattle virus

Listening-based tasks in TOEFL training

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Sino-US English Teaching 2009年第3期6卷 12-17页

作者： liu shu-mei Foreign Languages School Tianjin University of Commerce Tianjin 300134 China

There has been an increasing number of Chinese students studying in the international program of cooperative schools. They gain more chances of learning diverse cultures at the program, meanwhile they must meet the minimum requirement, taking part in TOEFL IBT and reaching a certain level. Therefore, how to prepare for the TOEFL IBT is the most important task for the students pursuing their further study. This article mainly focuses on the features ofTOEFL IBT and the strategies of learning it.

关键词： listening-based tasks TOEFL IBT integrated skills learning strategies

Study on carbon emission calculation method and carbon reduction indexes of urban building stock

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Science China(Technological Sciences) 2024年第9期67卷 2706-2726页

作者： YANG liu ZHANG Nan LU mei LUO ZhiXing DOU mei State Key Laboratory of Green Building Xi’an University of Architecture and TechnologyXi’an 710055China School of Architecture Xi’an University of Architecture and TechnologyXi’an 710055China School of Language Literature and LawXi’an University of Architecture and TechnologyXi’an 710055China School of Management Xi’an University of Architecture and TechnologyXi’an 710055China China Southwest Architectural Design and Research Institute Corp.Ltd. Chengdu 610041China

Carbon emissions associated with buildings are a major source of urban emissions. To put forward the methods and strategies to curb carbon emissions from urban building stock, it is not only necessary to establish a carbon emission calculation method for fine statistical analysis, but also to evaluate carbon emissions of urban planning schemes with applicable indexes. Currently,researches mainly focus on carbon emissions of individual buildings. When expanded to urban building stock, the calculation faces the lack of basic data, inadequate spatial analysis and unspecific carbon reduction indexes. Therefore, this study proposes a bottom-up calculation method for urban building stock, conducts spatial analysis based on carbon balance of urban grids, reveals the coupling mechanism between urban carbon reduction indexes and grid carbon emissions, and systematically establishes a carbon-reduction-oriented urban planning method that comprises calculation, analysis and evaluation, which is applied to Xi'an,China. This study provides a theoretical reference for cities to formulate carbon reduction targets and implement planning strategies by evaluating and predicting carbon emissions from urban building stock.

关键词： carbon emissions urban building stock calculation method carbon reduction index

The IDD Transcription Factors:Their Functions in Plant Development and Environmental Response

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Phyton-International Journal of Experimental Botany 2024年第1期93卷 63-79页

作者： Jing liu Defeng shu Zilong Tan mei Ma Huanhuan Yang Ning Guo Shipeng Li Dayong Cui School of Life Sciences Qilu Normal UniversityJinan250200China School of Life Sciences Shandong Normal UniversityJinan250014China

INDETERMINATE-DOMAIN proteins(IDDs)are a plant-specific transcription factor family characterized by a conserved ID domain with four zinc finger *** studies have demonstrated that IDDs coordinate a diversity of physiological processes and functions in plant growth and development,including floral transition,plant architecture,seed and root development,and hormone *** this review,we especially summarized the latest knowledge on the functions and working models of IDD members in Arabidopsis,rice,and maize,particularly focusing on their role in the regulatory network of biotic and abiotic environmental responses,such as gravity,temperature,water,and *** these mechanisms underlying the function of IDD proteins in these processes is important for improving crop yields by manipulating their ***,the review offers valuable insights into the functions and mechanisms of IDD proteins in plants,providing a foundation for further research and potential applications in agriculture.

关键词： INDETERMINATE DOMAIN flowering time root development shoot gravitropism plant immunity hormonal signaling environmental responses

Numerical simulations for radon migration and exhalation behavior during measuring radon exhalation rate with closed-loop method

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Nuclear Science and Techniques 2024年第1期35卷 81-95页

作者： Ming Xia Yong-Jun Ye shu-Yuan liu School of Resources Environment and Safety Engineering University of South ChinaHengyang 421001China Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy University of South ChinaHengyang 421001China

Accurate measurements of the radon exhalation rate help identify and evaluate radon risk regions in the *** these measurement methods,the closed-loop method is frequently ***,traditional experiments are insufficient or cannot analyze the radon migration and exhalation patterns at the gas–solid interface in the accumulation *** CFD-based technique was applied to predict the radon concentration distribution in a limited space,allowing radon accumulation and exhalation inside the chamber intuitively and *** this study,three radon exhalation rates were defined,and two structural ventilation tubes were designed for the *** consistency of the simulated results with the variation in the radon exhalation rate in a previous experiment or analytical solution was *** effects of the vent tube structure and flow rate on the radon uniformity in the chamber;permeability,insertion depth,and flow rate on the radon exhalation rate and the effective diffusion coefficient on back-diffusion were *** on the results,increasing the inser-tion depth from 1 to 5 cm decreased the effective decay constant by 19.55%,whereas the curve-fitted radon exhalation rate decreased(lower than the initial value)as the deviation from the initial value increased by approximately 7%.Increasing the effective diffusion coefficient from 2.77×10^(-7) to 7.77×10^(-6) m^(2) s^(-1) made the deviation expand from 2.14 to 15.96%.The conclusion is that an increased insertion depth helps reduce leakage in the chamber,subject to notable back-diffusion,and that the closed-loop method is reasonably used for porous media with a low effective diffusion coefficient in view of the back-diffusion *** CFD-based simulation is expected to provide guidance for the optimization of the radon exhalation rate measurement method and,thus,the accurate measurement of the radon exhalation rate.

关键词： Radon exhalation Numerical simulation Accumulation chamber

Exosome-Transmitted miR-224-5p Promotes Colorectal Cancer Cell Proliferation via Targeting ULK2 in p53-Dependent Manner

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Biomedical and Environmental Sciences 2024年第1期37卷 71-84页

作者： YANG Le mei ZHENG Qi liu Xiao Jia LI Xian Xian Veronica Lim CHEN Qi ZHAO Zhong Hua WANG shu Yang Department of Pathology School of Basic MedicineFudan UniversityShanghai 200032China Department of Pathology Dunedin School of MedicineUniversity of OtagoDunedin 9054New Zealand Department of Pathology Huashan HospitalFudan UniversityShanghai 200040China Shanghai Cancer Institute Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Teladoc Health Inc Shanghai 200335China Shanghai Medical College Fudan UniversityShanghai 200032China Shanghai Key Laboratory of Medical Imaging Computing and Computer Assisted Intervention Shanghai 200032China

Objective To investigate the role and molecular mechanism of exosomal miR-224-5p in colorectal cancer(CRC).Methods The miR-224-5p expression in CRC patient tissues and cell-derived exosomes was measured by laser capture microdissection and qRT-PCR,***-luciferase reporter gene assay was used to determine the target gene of *** protein expressions of p53 and unc-51 like kinase 2(ULK2)in CRC cells were detected by western *** cytometry was used to detect cell cycle and *** proliferation was measured by CCK8 and EdU *** The miR-224-5p expression was upregulated in CRC tissues and increased progressively with the rise of CRC *** cells secreted extracellular miR-224-5p mainly in an exosome-dependent manner,and then miR-224-5p could be transferred to surrounding tumor cells to regulate cell proliferation in the form of autocrine or ***,ULK2 was characterized as a direct target of miR-224-5p and was downregulated in CRC ***,ULK2 inhibited CRC cell proliferation in a p53-dependent ***,exosome-derived miR-224-5p partially reversed the proliferation regulation of ULK2 on CRC *** Our findings demonstrate that exosome-transmitted miR-224-5p promotes p53-dependent cell proliferation by targeting ULK2 in CRC,which may offer promising targets for CRC prevention and therapy.

关键词： miR-224-5p Exosome ULK2 p53 Cell proliferation Colorectal cancer

Improved length of calcium sulfate crystal seeds and whiskers via ball milling and hydration treatment

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Chinese Journal of Chemical Engineering 2024年第7期71卷 102-109页

作者： Yuke Li Yuxin liu Jizhen Huang Yi mei Faculty of Chemical Engineering Kunming University of Science and TechnologyKunming 650500China Yunnan Provincial Key Laboratory of Energy Saving in Phosphorus Chemical Engineering and New Phosphorus Materials Kunming University of Science and TechnologyKunming 650500China

Elucidating the effect of growth periods on the quality of calcium sulfate whiskers(CSWs)prepared from calcium sulfate dihydrate(DH)is ***,crystal seeds and whiskers were prepared from DH in a water–glycerol *** whiskers were obtained from crystal seeds prepared via hydration of DH for 30 s than via ball milling for 5 min followed by hydration for 20 *** attachment of cetyltrimethyl ammonium bromide and glycerol additives to the whisker tops promoted whisker *** whisker sponges exhibited good thermal barrier properties and compression cycle stability.

关键词： Hemihydrate gypsum whisker Hydration Ball milling/hydration Crystal seed Growth process

一种面向网络连通性的关键网络元素脆弱性分析方法

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控制与决策 2020年第6期35卷 1421-1426页

作者：刘树美于尧郭磊东北大学计算机科学与工程学院沈阳110169

准确评估网络系统脆弱性对于网络安全规划和风险管理至关重要.现有网络脆弱性分析方法大多利用单一特性识别脆弱元素,随着网络系统复杂化进程加快,多元化的脆弱性识别显得尤为重要.从攻击者的角度出发,提出一种面向网络连通性的关键元... 详细信息

准确评估网络系统脆弱性对于网络安全规划和风险管理至关重要.现有网络脆弱性分析方法大多利用单一特性识别脆弱元素,随着网络系统复杂化进程加快,多元化的脆弱性识别显得尤为重要.从攻击者的角度出发,提出一种面向网络连通性的关键元素脆弱性分析方法,识别网络中具有多重重要身份且破坏代价小的网络元素,利用局部分析措施确定网络关键元素并将网络连通性作为脆弱性衡量指标,以识别出关键元素中可致使网络连通性特定降级的最小代价集合.仿真结果表明,所提出方案对脆弱元素的定位更加准确,可为网络安全防护措施的制定提供有效且可靠的参考.

关键词：网络安全网络脆弱性分析连通性关键元素破坏代价

Two-component dimers of ultracold atoms with center-of-mass-momentum dependent interactions

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Communications in Theoretical Physics 2024年第4期76卷 153-157页

作者： Yaru liu shu Yang Peng Zhang Department of Physics Renmin University of ChinaBeijing 100872China Graduate School of China Academy of Engineering Physics Beijing 100193China Key Laboratory of Quantum State Construction and Manipulation(Ministry of Education) Renmin University of ChinaBeijing 100872China

In a previous paper[***.A95,060701(R)(2017)],we demonstrated that a new type of two-body interaction,which depends on the center of mass(CoM)momentum,can be realized for ultracold atoms via laser-modulated magnetic Feshbach resonance(MFR).Further studies(e.g.L He et al,***.120,045302(2018))show that various interesting phenomena,such as Fulde–Ferrell superfluids,can be induced by scattering between ultracold atoms with this *** this work we investigate the shallow bound states of two ultracold atoms with this type of *** show that when the magnetic field B is below the MFR point B0,two shallow bound states can appear in this ***,a'two-component dimer'or a dimer with pseudo-spin 1/2 can be formed by two ***,the dispersion curve of the dimer may have either single or double minimums in the CoM momentum *** latter case can be explained as a result from significant pseudo-spin-orbital coupling(SOC)*** results show that the ultracold gases with CoM momentum dependent interaction may be a candidate for quantum simulations with ultracold two-component molecules,especially the molecule gases with SOC.

关键词： center-of-mass-momentum dependent interaction Raman-laser modulated MFR two-component dimer