检索结果-南通市图书馆

Establishment of a transformation system in close relatives of wheat under the assistance of TaWOX5

Journal of Integrative Agriculture 2024年第6期23卷 1839-1849页

作者： Yanan Chang Junxian liu Chang liu Huiyun liu Huali Tang Yuliang Qiu Zhishan Lin Ke Wang Yueming Yan Xingguo ye Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environment Improvement/College of Life Science Capital Normal UniversityBeijing 100048China College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Cell and Chromosome Engineering/Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China

Species closely related to wheat are important genetic resources for agricultural production,functional genomics studies and wheat *** this study,a wheat gene related to regeneration,TaWOX5,was applied to establish the Agrobacterium-mediated transformation systems of Triticum monococcum,hexaploid triticale,and rye(Secale cereale L.)using their immature *** plants were efficiently *** the transformation process,the Agrobacterium infection efficiency was assessed by histochemical staining forβ-glucuronidase(GUS).Finally,the transgenic nature of regenerated plants was verified by polymerase chain reaction(PCR)-based genotyping for the presence of the GUS and bialaphos resistance(bar)genes,histochemical staining for GUS protein,and the QuickStix strip assay for bar *** transformation efficiency of *** genotype PI428182 was 94.4%;the efficiencies of four hexaploid triticale genotypes Lin456,ZS3297,ZS1257,and ZS3224 were 52.1,41.2,19.4,and 16.0%,respectively;and the transformation efficiency of rye cultivar Lanzhou Heimai was 7.8%.Fluorescence in situ hybridization(FISH)and genomic in situ hybridization(GISH)analyses indicated that the GUS transgenes were integrated into the distal or near centromere(proximal)regions of the chromosomes in transgenic *** and hexaploid triticale *** the transgenic hexaploid triticale plants,the foreign DNA fragment was randomly integrated into the AABB and RR ***,the transgene was almost stably inherited in the next generation by Mendel’s *** findings in this study will promote the genetic improvement of the three plant species for grain or forage production and the improvement of cereal species including wheat for functional genomics studies.

关键词： Triticum monococcum hexaploid triticale rye TaWOX5 Agrobacterium transformation efficiency

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Special electromyographic features in a child with paramyotonia congenita: A case report and review of literature

引用

World Journal of Clinical Cases 2024年第3期12卷 587-595页

作者： Hui Yi Chen-Xiang liu Shu-Xin ye Yu-Lin liu Department of Neuroelectrophysiology Qilu Children’s Hospital of Shandong UniversityJinan 250000Shandong ProvinceChina Department of Neuroelectrophysiology University of Health and Rehabilitation Sciences(Qingdao Municipal Hospital)Qingdao 266000Shandong ProvinceChina

BACKGROUND Paramyotonia congenita(PMC)stands as a rare sodium channelopaty of skeletal muscle,initially identified by *** identification of PMC often relies on electromyography(EMG),a diagnostic *** child’s needle EMG unveiled trains of myotonic discharges with notably giant amplitudes,alongside irregular wave trains of myotonic *** distinctive observation had not surfaced in earlier *** SUMMARY We report the case of a 3-year-old female child with PMC,who exhibited la-ryngeal stridor,muffled speech,myotonia from ***,exposure to cool water,crying,and physical activity exacerbated the myotonia,which was relieved in warmth,yet never *** myotonia was observable in bilateral *** symptoms remained unchanged after potassium-rich food consumption like *** periodic paralysis was *** magnetic resonance imaging yielded normal *** potassium remained within normal range,while creatine kinase showed slight ***-wide genetic testing pinpointed a heterozygous mutation on chromosome SCN4A:c.3917G>A(p.G1306E).After a six-month mexiletine regimen,symptoms *** In this case revealed the two types of myotonic discharges,and had not been documented in other *** underscore two distinctive features:Giant-amplitude potentials and irregular waves.

关键词： Paramyotonia congenita Channelopathy Electromyography Child Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Prestoring lithium into SnO_(2)coated 3D carbon fiber cloth framework as dendrite-free lithium metal anode

引用

Particuology 2024年第1期84卷 89-97页

作者： Tao Wei Yanyan Zhou Cheng Sun Lesheng liu Sijia Wang Mengting Wang ye liu Qing Huang Quanchao Zhuang Yongfu Tang School of Energy and Power Jiangsu University of Science and TechnologyZhenjiang212003China College of Materials Science and Engineering Nanjing Forestry UniversityNanjing210037China Li-ion Batteries Lab School of Materials Science and EngineeringChina University of Mining and TechnologyXuzhouChina Hebei Key Laboratory of Applied Chemistry School of Environmental and Chemical EngineeringYanshan UniversityQinhuangdao066004China

For several decades,the promise of implementing of lithium(Li)metal anodes has been regarded as the"holy grail"for Li-based ***,we have proposed a facile design of a carbon fiber cloth(CFC)framework coated with SnO_(2)nanoparticles through a hydrothermal process,which served as a reliable host for prestoring molten Li to produce a CFC@SnO_(2)@Li composite ***,TEM,HRTEM,XPS and different electrochemical characterizations were carried *** to the synergetic effects of the 3D conductive CFC and the coated lithiophilic SnO_(2)nanoparticles,the designed CFC@SnO_(2)@Li electrodes can buffer the volume changes and reduce the local current density,thus suppress the Li dendrites during ***,the CFC@SnO_(2)electrodes showed a high and stable CE of 98.6%for 1000 cycles at a current density of 1 mA cm^(-2)(1 mAh cm^(-2)).What is more,at a high current density of 5 mA cm^(-2)and a high areal capacity of 5 mAh cm^(-2),the symmetric cell displayed relatively low overpotential and long cycling lifetime of 1600 *** results confirm its great potential as lithium metal anodes in practical battery applications.

关键词： Lithiophilic Lithium metal anodes SnO_(2) 3D anode Dendrite-free

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A cost-efficient quantum access network with qubit-based synchronization

引用

Science China(Physics,Mechanics & Astronomy) 2024年第4期67卷 12-22页

作者： Chunfeng Huang ye Chen Tingting Luo Wenjie He Xin liu Zhenrong Zhang Kejin Wei Guangxi Key Laboratory for Relativistic Astrophysics School of Physical Science and TechnologyGuangxi UniversityNanning 530004China Guangxi Key Laboratory of Multimedia Communications and Network Technology School of Computer Electronics and InformationGuangxi UniversityNanning 530004China

Quantum key distribution(QKD)is a physical layer encryption technique that enables two distant parties to exchange secure keys with information-theoretic *** the last two decades,QKD has transitioned from laboratory research to real-world applications,including multi-user quantum access networks(QANs).This network structure allows users to share a single-photon detector at a network node through time-division multiplexing,thereby significantly reducing the network ***,current QAN implementations require additional hardware for auxiliary tasks such as time *** address this issue,we propose a cost-efficient QAN that uses qubit-based *** this approach,the transmitted qubits facilitate time synchronization,eliminating the need for additional synchronization *** tested our scheme by implementing a network for two users and successfully achieved average secure key rates of 53.84 kbps and 71.90 kbps for each user over a 50-km commercial fiber *** addition,we investigated the capacity of the access network under cross-talk and loss *** simulation results demonstrate that this scheme can support a QAN with 64 users with key rates up to 1070 *** work provides a feasible and cost-effective way to implement a multi-user QKD network,further promoting the widespread application of QKD.

关键词： quantum key distribution quantum access network qubit-based synchronization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

NF-κB in biology and targeted therapy:new insights and translational implications

引用

Signal Transduction and Targeted Therapy 2024年第4期9卷 1322-1358页

作者： Qing Guo Yizi Jin Xinyu Chen Xiaomin ye Xin shen Mingxi Lin Cheng Zeng Teng Zhou Jian Zhang Department of Medical Oncology Fudan University Shanghai Cancer CenterNo.270Dong'an RoadShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghaiChina State Key Laboratory of Oncogenes and Related Genes Renj-Med-X Stem Cell Research CenterShanghai Cancer Institute&Department of UrologyRen Ji HospitalSchool of Medicine and School of Biomedical EngineeringShanghai Jiao Tong UniversityShanghai 200127PR China Department of Cardiology the First Affiliated Hospital of Sun Yat-Sen University58 Zhongshan 2nd RoadGuangzhou 510080China Department of Neurology Rujin HospitalShanghai JiaoTong University Schoolof MedicineShanghaiChina

NF-κB signaling has been discovered for nearly 40 ***,NF-κB signaling was identified as a pivotal pathway in mediating inflammatory ***,with extensive and in-depth investigations,researchers have discovered that its role can be expanded to a variety of signaling mechanisms,biological processes,human diseases,and treatment *** this review,we first scrutinize the research process of NF-κB signaling,and summarize the composition,activation,and regulatory mechanism of NF-κB *** investigate the interaction of NF-κB signaling with other important pathways,including PI3K/AKT,MAPK,JAK-STAT,TGF-β,Wnt,Notch,Hedgehog,and TLR *** physiological and pathological states of NF-κB signaling,as well as its intricate involvement in inflammation,immune regulation,and tumor microenvironment,are also ***,we illustrate how NF-κB signaling is involved in a variety of human diseases,including cancers,inflammatory and autoimmune diseases,cardiovascular diseases,metabolic diseases,neurological diseases,and ***,we discuss the therapeutic approaches targeting NF-κB signaling,including IKK inhibitors,monoclonal antibodies,proteasome inhibitors,nuclear translocation inhibitors,DNA binding inhibitors,TKls,non-coding RNAs,immunotherapy,and ***,we provide an outlook for research in the field of NF-κB *** hope to present a stereoscopic,comprehensive NF-κB signaling that will inform future research and clinical practice.

关键词： PI3K/AKT diseases involvement

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Functional characterization,structural basis,and protein engineering of a rare flavonoid 2′-O-glycosyltransferase from Scutellaria baicalensis

引用

Acta Pharmaceutica Sinica B 2024年第8期14卷 3746-3759页

作者： Zilong Wang Xueqing Du Guo ye Haotian Wang Yizhan liu Chenrui liu Fudong Li HansÅgren Yang Zhou Junhao Li Chao He De-An Guo Min ye State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Anhui Key Laboratory of Modern Biomanufacturing and School of Life Sciences Anhui UniversityHefei 230601China National Science Center for Physical Sciences at Microscale Division of Molecular&Cell Biophysics and School of Life Sciences University of Science and Technology of ChinaHefei 230026China Department of Physics and Astronomy Uppsala UniversityUppsala SE-75120Sweden School of Pharmacy Jinan UniversityGuangzhou 510632China Shanghai Research Center for Modernization of Traditional Chinese Medicine Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Glycosylation is an important post-modification reaction in plant secondary metabolism,and contributes to structural diversity of bioactive natural *** plants,glycosylation is usually catalyzed by *** 2′-O-glycosides are rare ***,no UGTs have been reported,thus far,to specifically catalyze 2′-O-glycosylation of *** this work,UGT71AP2 was identified from the medicinal plant Scutellaria baicalensis as the first flavonoid 2′-*** could preferentially transfer a glycosyl moiety to 2′-hydroxy of at least nine flavonoids to yield six new *** of the 2′-O-glycosides showed noticeable inhibitory activities against cyclooxygenase *** crystal structure of UGT71AP2(2.15Å)was solved,and mechanisms of its regio-selectivity was interpreted by pKa calculations,molecular docking,MD simulation,MM/GBSA binding free energy,QM/MM,and hydrogen‒deuterium exchange mass spectrometry *** structure-guided rational design,we obtained the L138T/V179D/M180T mutant with remarkably enhanced regio-selectivity(the ratio of 7-O-glycosylation byproducts decreased from 48%to 4%)and catalytic efficiency of 2′-O-glycosylation(kcat/Km,0.23 L/(s·μmol),12-fold higher than the native).Moreover,UGT71AP2 also possesses moderate UDP-dependent de-glycosylation activity,and is a dual function *** work provides an efficient biocatalyst and sets a good example for protein engineering to optimize enzyme catalytic features through rational design.

关键词： Glycosyltransferase Regio-selectivity Crystal structure De-glycosylation Catalytic mechanisms

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Downstream Competition and Upstream Innovation:Theory and Evidence from China

引用

China Finance and Economic Review 2024年第1期13卷 67-87页

作者： Jing Dai Guanchun liu Ziqi ye Haiou Mao Yongwei ye School of Finance Hubei University of Economics Lingnan College Sun Yatsen University School of Business Administration Zhongnan University of Economics and Law College of Economics and Management Huazhong Agricultural University School of Public Economics and Management Shanghai University of Finance and Economics

Competition is the catalyst of corporate innovation,which is the foundation of a country's core *** the view of vertical industrial chain,this paper examines how downstream competition affects upstream firms'innovation *** innovation helps reduce the production cost of each product,increasing demand of intermediate products caused by downstream competition enhances the marginal revenue of upstream firms'innovation,thus leading to an innovation incentive *** identify causality,we utilize China's WTO accession,a quasinatural experiment,to calculate downstream competition combined with import tariff reduction and input-output table,and then run a difference-in-difference *** the data of Annual Survey of Industrial Firms and State Intellectual Property Office,our results find that downstream competition has a significantly positive impact on upstream firms'innovation,and this effect is more pronounced in firms with less industry competition,greater initial innovation capacity and weaker financial ***,the number of firms,the degree of industry competition and the scale of intermediate products in the downstream sector increase,confirming the market size *** sum,our findings document that downstream competition can motivate upstream firms to innovate through vertical industrial chain,providing some useful insights for China to build a fair market competition environment and promote innovation-driven growth regime.

关键词： downstream competition upstream firms'innovation vertical industrial chain market size effect

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Recent Progress in Optical Sensors Based on MXenes Quantum Dots and MXenes Nanosheets

引用

Journal of Analysis and Testing 2024年第1期8卷 95-113页

作者： Chunxiao Lin Xinhong Song Weilan ye Ting liu Mingcong Rong Li Niu Guangzhou Key Laboratory of Sensing Materials and Devices/Center for Advanced Analytical Science/School of Chemistry and Chemical Engineering Guangzhou UniversityGuangzhou 510006China College of Ocean Science and Engineering Shanghai Maritime UniversityShanghai 201306China

In recent years,MXenes,as a new promising two-dimensional material,have received extensive attentions in the fields of environmental remediation,catalysts,sensors,and energy *** broaden the applications of MXenes in sensing,MXenes nanosheets(MNSs) and MXenes quantum dots(MQDs) are prepared by physical etching or chemical *** are monolayered or multilayered MXenes nanosheets within several nanometers in height,and can serve as the perfect reaction substrate and fluorescence *** are spherical,fluorescent MXenes QDs within several nanometers in size,which possess favorable stability and photo *** the distinct features such as high conductivity,favorable hydrophilicity,biocompatibility,abundant surface functional groups,and easy functionalization,MNSs and MQDs show potential applications in the fields of anti-counterfeiting,environmental monitoring,biosensing and *** review focuses on the research of MNS s/MQDs-based optical sensors,including colorimetric sensors,fluorescence sensors,electrochemiluminescence sensors,surface plasmon resonance sensors,and surface-enhanced Raman scattering ***,the current challenges and prospects concerning MNSs/MQDs-based optical sensing techniques are discussed.

关键词： MXenes quantum dots Fluorescence Colorimetry Electrochemiluminescence Surface plasmon resonance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Multi-stimuli-responsive aggregation-induced emission copper iodide cluster

引用

Science China Chemistry 2024年第4期67卷 1193-1197页

作者： Bo Li Yuan-Yuan liu Peng Luo ye-Ru liu Ying-Jun Chen Kai Li Shuang-Quan Zang College of Chemistry and Pharmacy Engineering Nanyang Normal UniversityNanyang 473061China Green Catalysis Center and College of Chemistry Zhengzhou UniversityZhengzhou 450001China

Stimuli-responsive luminescent materials have attracted significant attention in the development of smart photoactive materials for both fundamental research and technological *** this work,a new copper iodide cluster(1)with aggregationinduced emission(AIE)characteristic,was obtained combining Cu_(4)I_(4) core with rhodamine B derivative ligand.1 has reversible and distinct multi-stimuli-responsive luminescence for external temperature,volatile organic compound,and mechanical ***,1 exhibited unusual large blue shift(84 nm)after being ground,which originated from the change of intermolecular ***,1 exhibits high oxygen quenching efficiency for 82.2% at 1 *** studies showed that the multi-stimuli-responsive luminescence properties of 1 can be attributed to the regulation of cluster-centered luminescence process,metal-to-ligand charge transfer process,halide-to-metal charge transfer luminescence process and aggregationinduced barrier to oxygen *** work not only reports an AIE copper iodide cluster,but also provides a new strategy to develop multi-stimuli-responsive luminescence materials.

关键词： copper iodide cluster aggregation-induced emission multi-stimuli-response

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The enhanced mechanical properties of SiC nanowires/Ba_(0.75)Sr_(0.25)Al_(2)Si_(2)O_(8) ceramics with embedded SiO_(2) interface

引用

Journal of Advanced Ceramics 2024年第7期13卷 933-941页

作者： Xin Li Xiaomeng Fan Haojie Luo Xiaoke Lu Jimei Xue Fang ye Yongsheng liu Science and Technology on Thermostructural Composite Materials Laboratory Northwestern Polytechnical UniversityXi’an 710072China Frontier Institute of Science and Technology Xi’an Jiaotong UniversityXi’an 710049China

In this work,low-softening-temperature and low-modulus SiO_(2) was introduced as an embedded interface between SiC nanowires(SiCnws)and a Ba_(x)Sr_(1−x)Al_(2)Si_(2)O_(8) ceramic matrix to enhance strength and toughness of the *** the sintering process,molten SiO_(2) enhances the flowability of the ceramic powders and modifies the dispersion of *** strengthening effect of SiCnws was fully realized,and the flexural strength of the optimized ceramics reached 193±16 MPa,which represents an increase of 52.6%.After the formation of the embedded SiO_(2) interface with a low modulus,cracks can deflect along the SiCnws surface,which is consistent with the criterion of He and *** can effectively extend the crack propagation path,and the fracture toughness(K_(IC))is thus improved by 94.0%,reaching 3.1±0.5 MPa·m^(1/2).

关键词： multiphase ceramics SiO_(2) interface densification mechanical properties

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：