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山羊circFDXR的鉴定及其在卵巢颗粒细胞中高效表达的研究

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中国畜牧兽医 2024年第6期51卷 2330-2341页

作者：刘杰李之瀚郭聪慧冯光杭薛文哲刘德武柳广斌孙宝丽郭勇庆邓铭邹娴李耀坤华南农业大学动物科学学院广州510642 华南农业大学畜禽育种国家地方联合工程研究中心广州510642 华南农业大学广东省农业动物基因组学与分子育种重点实验室广州510642 广东省农业科学院动物科学研究所畜禽育种国家重点实验室广东省畜禽育种与营养研究重点实验室广州510640

【目的】鉴定山羊环状RNA(circular RNA,circRNA)铁氧还蛋白还原酶(circFDXR)的表达,建立其在山羊原代卵巢颗粒细胞中的高效表达方式。【方法】利用PCR扩增、DNA测序、RNase R酶消化、实时荧光定量PCR鉴定circFDXR的环状性质。通过核质... 详细信息

【目的】鉴定山羊环状RNA(circular RNA,circRNA)铁氧还蛋白还原酶(circFDXR)的表达,建立其在山羊原代卵巢颗粒细胞中的高效表达方式。【方法】利用PCR扩增、DNA测序、RNase R酶消化、实时荧光定量PCR鉴定circFDXR的环状性质。通过核质分离、荧光原位杂交(FISH)检测circFDXR亚细胞定位。利用同源重组将circFDXR的线性序列无缝克隆至慢病毒载体pLC5-ciR,转染至293T细胞包装病毒后进行病毒滴度测定,并确定最佳感染复数(MOI),并以最佳MOI感染山羊原代卵巢颗粒细胞,通过实时荧光定量PCR检测过表达效率,并测序验证circFDXR是否正确环化。【结果】山羊circFDXR主要定位于卵巢颗粒细胞的细胞质中,耐受RNase R酶的消化。成功构建circFDXR慢病毒过表达载体,浓缩病毒滴度为5.49×10~9 TU/mL,感染山羊卵巢颗粒细胞的最佳MOI为100。实时荧光定量PCR结果显示,与对照组相比,过表达组circFDXR表达量极显著升高(P<0.01),测序结果显示过表达的circFDXR可以正确环化。【结论】circFDXR主要定位于卵巢颗粒细胞的细胞质中,相比线性RNA具有更高的稳定性。利用慢病毒包装的circFDXR在山羊原代卵巢颗粒细胞中可以正确环化,本试验结果为进一步研究circFDXR在山羊原代卵巢颗粒细胞中的功能奠定了理论基础。

关键词：山羊 circFDXR 慢病毒包装卵巢颗粒细胞

硒化物在文蛤响应镉胁迫过程中作用的转录组学分析

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海洋科学 2024年第1期48卷 25-35页

作者：郑年昊孙铭姚琳琳荆圆圆张天文胡凡光王兴强刘广斌江苏海洋大学海洋生命与水产学院江苏连云港222005 山东省海洋科学研究院山东青岛266104

研究两种硒化物在文蛤富集、排出镉过程中的作用,对文蛤进行转录组测序,并进行生物信息学分析,以探讨硒化物对Cd^(2+)代谢相关机制。硒化卡拉胶组和亚硒酸钠组共产出587 855条高质量reads。基因本体分析功能注释到15 380条unigenes, KEG... 详细信息

研究两种硒化物在文蛤富集、排出镉过程中的作用,对文蛤进行转录组测序,并进行生物信息学分析,以探讨硒化物对Cd^(2+)代谢相关机制。硒化卡拉胶组和亚硒酸钠组共产出587 855条高质量reads。基因本体分析功能注释到15 380条unigenes, KEGG通路注释到18 866条unigenes。差异基因主要富集到肌球蛋白复合物(myosin complex)、离子通道抑制(ion channel suppression)等基因本体分析功能中。差异基因KEGG通路富集显示,在嘌呤代谢(purinemetabolism)、ECM受体相互作用(extracellularmatrix receptor interaction)、硒化合物代谢(metabolism of selenium compounds)等通路显著富集。从结果分析Cd^(2+)可以使文蛤体内蛋白转录修饰出现异常;有机硒可以通过调控金属离子通道活性来排出细胞内的Cd^(2+);无机硒主要作用于文蛤细胞表面,增强其表面活性抵御Cd^(2+)进入细胞;差异基因KEGG注释结果表明有机硒与无机硒在文蛤重金属代谢中作用机制以及途径不相同。

关键词：文蛤硒化卡拉胶亚硒酸钠转录组分析差异基因表达

某院2019-2021年度590例不良反应报告分析

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甘肃医药 2024年第6期43卷 536-540页

作者：何苗苗谢六生邓朝尹刘光斌王艳酒钢医院甘肃嘉峪关735100

目的:了解某院2019~2021年度药品不良反应(ADR)的发生情况及特点,为患者安全用药提供依据。方法:将2019年1月至2021年12月各临床科室上报至国家ADR监测网的590例ADR报告,从ADR报告类型、ADR报告来源、ADR报告临床科室、患者性别及年龄、... 详细信息

目的:了解某院2019~2021年度药品不良反应(ADR)的发生情况及特点,为患者安全用药提供依据。方法:将2019年1月至2021年12月各临床科室上报至国家ADR监测网的590例ADR报告,从ADR报告类型、ADR报告来源、ADR报告临床科室、患者性别及年龄、ADR给药途径、ADR怀疑药物种类、ADR累及器官和/或系统及主要临床表现、ADR发生时间、ADR治疗及转归等方面进行统计分析。结果:ADR可发生于任何年龄段,50岁以上较多,为355例(60.16%),引起DAR的药物种类主要为抗微生物药物182例(30.85%),发生ADR给药途径以静脉滴注为主,共416例(70.51%),ADR的临床表现主要是皮肤及其附件285例(48.31%)和胃肠道88例(14.92%)等。结论:ADR的发生与多种因素相关,医务人员应加强对ADR的监测与报告、合理使用抗微生物药物、最大限度减少静脉滴注药物使用的同时,应更加重视儿童、孕妇及肿瘤患者的用药监护,降低ADR发生风险,促进临床安全合理用药。

关键词：药品不良反应报告分析用药安全

2008—2022年天津市南开区梅毒报告病例流行病学特征分析

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职业与健康 2024年第12期40卷 1632-1635页

作者：郑雅静柳光斌王楠张亭侯莹孙健华天津市南开区疾病预防控制中心性病艾滋病科天津市南开区疾病预防控制中心办公室

目的分析天津市南开区梅毒流行病学特征,为性病防治工作提供依据。方法以中国疾病预防控制信息系统数据为基础,使用描述统计学方法计算报告发病率、构成比,用χ2检验进行率的比较和趋势χ2检验分析其变化趋势,分析2008—2022年天津市南... 详细信息

目的分析天津市南开区梅毒流行病学特征,为性病防治工作提供依据。方法以中国疾病预防控制信息系统数据为基础,使用描述统计学方法计算报告发病率、构成比,用χ2检验进行率的比较和趋势χ2检验分析其变化趋势,分析2008—2022年天津市南开区梅毒病例报告情况。结果2008—2022年天津市南开区累计报告梅毒病例4844例,年均报告发病率为29.83/10万。梅毒报告病例总体呈下降趋势,由2008年的40.54/10万降至2022年的23.45/10万(χ2趋势=69.858,P<0.01),其中一期、二期梅毒逐年下降,分别年均下降12.01%和17.07%,但隐性梅毒发病率逐年上升,由10.94/10万增至20.27/10万(χ2趋势=212.834,P<0.01),年均增长3.84%。南开区全年各月均有梅毒病例报告,其中2月份病例最少,3月份出现发病小高峰,病例主要集中在7—9月,共1386例,占28.61%。梅毒报告病例中,男性2642例(54.54%),发病率为32.15/10万,女性2202例(45.46%),发病率为27.46/10万。报告病例主要集中在≥20岁人群,其中20~<30岁病例数最多,为1273例(26.28%),<50岁各年龄组发病率均呈逐年下降的趋势,而≥60岁年龄组发病率呈上升趋势,由2008年的28.49/10万上升至2022年的35.04/10万(χ~2_(趋势)=15.399,P<0.01)。梅毒报告病例职业分布以家务及待业、离退人员、商业服务人员为主,报告科室分布以皮肤(性病)科病例最多,为331例(32.77%)。结论天津市南开区梅毒发病情况总体呈下降趋势,但梅毒疫情防控形势依然严峻,建议将梅毒防治与艾滋病防治工作相结合,开展综合防治工作。

关键词：梅毒报告病例流行病学特征

Limited-Budget Consensus Design and Analysis for Multiagent Systems With Switching Topologies and Intermittent Communications

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IEEE/CAA Journal of Automatica Sinica 2021年第10期8卷 1724-1736页

作者： Le Wang Jianxiang Xi Bo Hou guangbin liu Force University of Engineering Xi’an 710025China

This paper investigates limited-budget consensus design and analysis problems of general high-order multiagent systems with intermittent communications and switching *** main contribution of this paper is that the tradeoff design between the energy consumption and the consensus performance can be realized while achieving leaderless or leaderfollowing consensus,under constraints of limited budgets and intermittent ***,a new intermittent limitedbudget consensus control protocol with a practical trade-off design index is proposed,where the total budget of the whole multiagent system is ***,leaderless limited-budget consensus design and analysis criteria are derived,in which the matrix variables of linear matrix inequalities are determined according to the total budget and the practical trade-off design ***,an explicit formulation of the consensus function is derived to describe the consensus state trajectory of the whole ***,a new two-stage transformation strategy is utilized for leader-following cases,by which the dynamics decomposition of leaderless and leader-following cases can be converted into a unified framework,and sufficient conditions of the leader-following limited-budget consensus design and analysis are determined via those of the leaderless ***,numerical simulations are given to illustrate theoretical results.

关键词： Intermittent communication limited-budget consensus multiagent system switching topology

Consensus of Second-Order Multi-Agent Systems with Time-Varying Delays and Antagonistic Interactions

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Tsinghua Science and Technology 2015年第2期20卷 205-211页

作者： Bo Hou Fuchun Sun Hongbo Li guangbin liu State Key Laboratory on Intelligent Technology and Systems Department of Computer Science and TechnologyTsinghua University High-Tech Institute of Xi’an

This study investigates the consensus problem of second-order multi-agent systems subject to time- varying interval-like delays. The notion of consensus is extended to networks containing antagonistic interactions modeled by negative weights on the communication graph. A unified framework is established to address both the stationary and dynamic consensus issues in sampled-data settings. Using the reciprocally convex approach, a sufficient condition for consensus is derived in terms of matrix inequalities. Numerical examples are provided to illustrate the effectiveness of the proposed result.

关键词： multi-agent systems second-order consensus cooperation and competition

Direct generation of Zn metal using laser-induced ZnS to eradicate carbon emissions from electrolysis Zn production

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Frontiers of Environmental Science & Engineering 2024年第1期18卷 83-95页

作者： Ying Chen Ning Duan Linhua Jiang Fuyuan Xu guangbin Zhu Yao Wang Yong liu Wen Cheng Yanli Xu State Key Laboratory of Pollution Control and Resources Reuse College of Environmental Science and EngineeringTongji UniversityShanghai 200092China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092China School of Environmental Science and Engineering Tianjin UniversityTianjin 300350China School of Materials Science and Engineering Anhui University of Science&TechnologyHuainan 232001China

In response to the goal of net-zero emissions proposed by Intergovernmental Panel on Climate Change, Chinese government has pledged that carbon emissions will peak by 2030, and achieve carbon neutrality by 2060. However, the high carbon energy structure of traditional industries has aggravated environmental problems, such as greenhouse effect and air pollution. The goal of carbon neutrality will be difficult to achieve without the development of disruptive theories and technologies. The electrolytic zinc industry requires high-temperature roasting at ~1000 ℃, generating large amounts of greenhouse gases and SO_(2). High concentrations of sulfuric acid (200 g/L) are subsequently used for electrolysis, and each ton of zinc produced generates 50 kg of anode slime with lead content of up to 16%, as well as 0.35 m3 of wastewater containing zinc and lead. To solve these problems, an optical metallurgy method is proposed in this study. The proposed method uses laser-induced photoreduction to decompose ZnS and reduce metal ions to metal. Results indicate that Zn0 and S8 can be detected on the surface of ZnS at a specific wavelength and laser fluence. The generation mechanism of Zn0 is such that laser induces an electronic transition that breaks ionic bond in ZnS, resulting in its decomposition and photoreduction to Zn0 under an inert argon gas atmosphere. This method does not reduce other metals in the mineral since it does not use high-temperature roasting, providing a new way of producing high-purity metal without greenhouse gas emissions and heavy metal pollution caused by traditional zinc electrolysis.

关键词： Laser metallurgy ZnS Photochemical reduction Zinc

Degradation data-driven approach for remaining useful life estimation

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Journal of Systems Engineering and Electronics 2013年第1期24卷 173-182页

作者： Zhiliang Fan guangbin liu Xiaosheng Si Qi Zhang Qinghua Zhang Department of Automation The Second Artillery Engineering University Department of Automation Tsinghua University Guangdong University of Petrochemical Technology

Remaining useful life （RUL） estimation is termed as one of the key issues in prognostics and health management （PHM）. To achieve RUL estimation for individual equipment, we present a degradation data-driven RUL estimation approach under the collaboration between Bayesian updating and expectation maximization （EM） algorithm. Firstly, we utilize an exponential-like degradation model to describe equipment degradation process and update stochastic parameters in the model via Bayesian approach. Based on the Bayesian updating results, both probability distribution of the RUL and its point estimation can be derived. Secondly, based on the monitored degradation data to date, we give a parameter estimation approach for non-stochastic parameters in the degradation model and prove that the obtained estimation is unique and optimal in each iteration. Finally, a numerical example and a practical case study for global positioning system （GPS） receiver are provided to show that the presented approach can model degradation process and achieve RUL estimation effectively and generate better results than a previously reported approach in literature.

关键词： reliability degradation remaining useful life (RUL) prognostics global positioning system (GPS).

Ultrasonic Nondestructive Signals Processing Based on Matching Pursuit with Gabor Dictionary

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Chinese Journal of Mechanical Engineering 2011年第4期24卷 591-595页

作者： GUO Jinku WU Jinying YANG Xiaojun liu guangbin Xi＇an Research Institute of High Technology Hongqing Town Xi＇an 710025 China Department of Automation Tsinghua University Beijing 100084 China

The success of ultrasonic nondestructive testing technology depends not only on the generation and measurement of the desired waveform, but also on the signal processing of the measured waves. The traditional time-domain methods have been partly successful in identifying small cracks, but not so successful in estimating crack size, especially in strong backscattering noise. Sparse signal representation can provide sparse information that represents the signal time-frequency signature, which can also be used in processing ultrasonic nondestructive signals. A novel ultrasonic nondestructive signal processing algorithm based on signal sparse representation is proposed. In order to suppress noise, matching pursuit algorithm with Gabor dictionary is selected as the signal decomposition method. Precise echoes information, such as crack location and size, can be estimated by quantitative analysis with Gabor atom. To verify the performance, the proposed algorithm is applied to computer simulation signal and experimental ultrasonic signals which represent multiple backscattered echoes from a thin metal plate with artificial holes. The results show that this algorithm not only has an excellent performance even when dealing with signals in the presence of strong noise, but also is successful in estimating crack location and size. Moreover, the algorithm can be applied to data compression of ultrasonic nondestructive signal.

关键词： ultrasonic signal processing sparse representation matching pursuit Gabor dictionary