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Extra-Large Magnetoresistance in Fe/In2O3 Granular Films

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Chinese Physics Letters 2002年第7期19卷 1004-1006页

作者： Huang Bao-xin, liu yi-Hua Department of Physics and State Key Laboratory of Crystal Materials Shandong University Ji\'nan 250100

Nanogranular Fe0.35/(In2O3)0.65 films with complex magnetic structure were prepared by the rf sputtering method. An extra-large magnetoresistance up to 506% was obtained at 2.2 K, which is two orders of magnitude larger than that obtained at room temperature. This is related to the interaction with the impurities influencing the local magnetization, which is quite different from the spin-dependent tunnelling effect at room temperature. The interspacing Fe atoms dispersed in the In2O3 matrix play an important role in the transportation properties of carriers at low temperature

关键词： Indium compounds

基于微滴式数字PCR检测牛病毒性腹泻病毒方法的建立及初步应用

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农业生物技术学报 2025年第1期33卷 190-199页

作者：刘芯怡李丹秦刚陈俊贞付强史慧君新疆农业大学动物医学学院新疆草食动物新药研究与创制重点实验室天康生物制药有限公司新疆巴楚县畜牧兽医站中国农业科学院西部农业研究中心

牛病毒性腹泻病毒(Bovine viral diarrhea virus, BVDV)是引起犊牛(Bos sp.)病毒性腹泻的主要病原之一，导致犊牛免疫力降低，患病率和死亡率升高。目前对BVDV常用的检测方法存在灵敏度较低、容易出现假阳性等状况。本研究基于微滴式数... 详细信息

牛病毒性腹泻病毒(Bovine viral diarrhea virus, BVDV)是引起犊牛(Bos sp.)病毒性腹泻的主要病原之一，导致犊牛免疫力降低，患病率和死亡率升高。目前对BVDV常用的检测方法存在灵敏度较低、容易出现假阳性等状况。本研究基于微滴式数字PCR(droplet digital PCR, ddPCR)技术，建立BVDV精准检测技术。首先针对BVDV 5'UTR基因保守序列区域设计引物和探针，合成BVDV 5'UTR基因并构建质粒标准品。使用BVDV质粒标准品分别建立实时荧光定量PCR(real-time quantitative PCR, qPCR)和ddPCR检测方法，并优化其反应参数和条件，分析其灵敏度、特异性和重复性，并进行了临床应用。结果显示，本研究建立的qPCR方法中，最佳引物和探针浓度分别为300和500 nmol/L，标准曲线呈良好的线性关系，最低检测下限为1×10～4 copies/μL，具有良好的特异性和重复性。建立的ddPCR方法中，最低检测下限为0.64 copies/μL，比qPCR灵敏性高15 625倍，该方法具有良好的特异性和重复性。临床检测了82份样品，ddPCR检出率为15.58%,qPCR检出率为14.63%。本研究建立的ddPCR方法比qPCR方法更加灵敏、高效、特异且重复性良好，为BVDV鉴别诊断提供了技术支持。

关键词：牛病毒性腹泻病毒(BVDV) 微滴式数字PCR(ddPCR) 实时荧光定量PCR(qPCR) 灵敏性特异性重复性

Capillary Electrophoretic Determination of Jinggangmycin A in Formulations Using Direct UV Detection

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Chinese Chemical Letters 2005年第11期16卷 1511-1514页

作者： Jin HE xin liu yi Shi liu Zi Niu YU National Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan 430070

A simple, fast and reliable method was developed for the analysis of jinggangmycin A （validamycin A） in commercial formulations. The running buffer used was acetate buffer （100 mmol/L, pH 4.7） with 15 kV as the applied voltage. The detection was achieved by using direct UV mode at 200 nm and the detection limit was 0.2 μg/mL. Linearity in the concentration range of 5-500 μg/mL was excellent （RE 〉 0.999）. The run-to-run repeatability （n = 3）, as expressed by the relative standard deviation （RSD） for migration times and peak areas were less than 0.5% and 3.0% respectively. The mean recovery ranged from 97.2% to 101.4%.

关键词： Jinggangmycin A validamycin A capillary zone electrophoresis direct UV detection.

A New Flavonoid from Oxytropis kansuensis

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Chinese Chemical Letters 2006年第12期17卷 1576-1578页

作者： Jun Xi liu xin yi HUANG Duo Long DI Sheng Xiang JIANG Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics Chinese Academy of Sciences Lanzhou 730000

A new flavonoid 1 rhamnocitrin-3-O-（6＂-femloyl）-β-D-galactopyranoside-4＇-O-β-D- glucospyranoside called kansuensisoside A together with a known flavonoid 2 （rhamnocitrin- 3-O-β-D-galactopyranoside-4＇-O-β-D-glucospyranoside） were isolated from the extract of the whole plant of Oxytropis kansuensis Bunge. The structure of 1 was elucidated by spectroscopic methods including 2D-NMR techniques （COSY, HMQC, HMBC） and HR-ESIMS.

关键词： Oxytropis kansuensis Bunge leguminosae flavonoid kansuensisoside A

Influence of Carbon-doped Content on Formation Conditions of Coesite

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Chemical Research in Chinese Universities 2009年第6期25卷 920-923页

作者： WANG De-jun liu Xiao-mei ZHU Cheng-jun YAN Duan-ting liu yi-xin SUN ying liu Fu-lin SU Wen-hui State Key Laboratory of Superhard Materials College of Physics Jilin University Changchun 130021 P. R. China Adult Education College Changchun University Changchun 130022 P. R. China Center of the Condensed Matter Science and Technology Harbin Institute of Technology Harbin 150001 P. R. China International Center for Material Physics Chinese Academy of Sciences Shenyang 110015 P. R. China

The mixtures of a-quartz and graphite powder with different mass ratios were, respectively, high-energetically mechanically milled and then treated under high pressure and high temperature. The influences of carbon content on the synthesis conditions of coesite were investigated. The experimental products were characterized by XRD, TEM, and Raman spectrometry. The results show that the existence of carbon can obviously inhibit the formation of coesite, and the higher the carbon content of the initial material, the higher the pressure for forming coesite.

关键词： Coesite High pressure Mechanical ball-milling α-SiO2 Graphite

5-aminolaevulinic Acid-photodynamic Therapy for the Treatment of Cervical Condylomata Acuminata

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Chinese Medical Sciences Journal 2009年第3期24卷 151-155页

作者： Yong-xin liu He-yi Zheng Xiu-rong liu Department of Dermatology and Venereology Peking Union Medical College Hospita Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Objective To investigate the efficacy and safety of photodynamic therapy （PDT） with topical 5-aminolaevulinic acid （ALA） on cervical condylomata acuminata. Methods Patients with cervical condylomata （n=30） were allocated into primary and recurrent group, and were given topical ALA under occlusive dressing for 3 hours followed by irradiation with semiconductor laser at a dose of 100 Jcm 2 and a power of 100 roW. The treatment was repeated 7 days later if the lesion was not completely removed after the first treatment. Complete response rate and recurrence rate of wart lesions as well as rate of adverse reaction were analyzed. Results The total complete response rate of PDT was 100% and the total recurrence rate was 5% after 3 months of follow-up. Recurrence rate of recurrent group was significantly lower than that of prior managements （100%, P〈0.01）. The side effects of PDT in patients mainly included mild burning and/or stinging restricted to the illuminated areas, and was significant lower than their own control （25% vs. 100%, P〈0.05）. Conclusion Compared with conventional therapies, topical application of ALA-PDT is a simple, effective, safe, well-tolerated, and low recurrence rate treatment for cervical condylomata acuminata.

关键词： 5-aminolaevulinic acid cervical condylomata acuminata photodynamic therapy

CpG island methylator phenotype in plasma is associated with hepatocellular carcinoma prognosis

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World Journal of Gastroenterology 2011年第42期17卷 4718-4724页

作者： Ji-Bin liu yi-xin Zhang Shu-Hui Zhou Min-xin Shi Jin Cai Yan liu Ke-Ping Chen Fu-Lin Qiang Institute of Life Sciences Jiangsu University Zhenjiang 212013 Jiangsu Province China

AIM： To evaluate the clinical significance of CpG island methylator phenotype （CIMP） in plasma and its association with hepatocellular carcinoma （HCC） progress. METHODS： CIMP status of 108 HCC patients was analyzed using a methylation marker panel in tumor tissues and plasma with methylation-specific polymerase chain reaction. Fifteen samples of non-neoplastic liver tissues and 60 of plasma from healthy persons were examined simultaneously. Examined genes included APC, WIF-1, RUNX-3, DI C-1, SFRP-1, DKK and E-cad.26/108, 24.07% in plasma; WIF-1, 53/108, 49.07% in tissue and 35/108, 32.41% in plasma; RUNX-3, 52/108, 48.14% in tissue and 42/108, 38.89% in plasma; DIC-1, 38/108, 35.18% in tissue and 23/108, 21.30% in plasma; SFRP-1, 40/108, 37.04% in tissue and 31/108, 28.7% in plasma; DKK, 39/108, 36.1% in tis- sue and 25/108, 23.14% in plasma; and E-cad, 37/108, 34.3% in tissue and 18/108, 16.67% in plasma. CIMP＋（≥3 methylated genes） was detected in 68 （60.2%） tumor tissue samples and 62 （57.4%） plasma samples. CIMP was not detected in non-neoplastic liver tissues or plasma of healthy persons. CIMP status in tumor tissues differed significantly in gender, hepatitis B surface antigen, alpha-fetoprotein, and tumor-node- metastasis stage （P 〈 0.05）. Similar results were obtained with plasma samples （P 〈 0.05）. There was no difference in CIMP status in age, presence of hepatitis C virus antibody, cirrhosis, number of nodes, number of tumors, tumor size, or Edmondson-Steiner stage. A one-year follow-up found that the metastatic rate and recurrence rate in the CIMP＋ group were significantly higher than in the CIMP- group as assessed with plasma samples （P 〈 0.05）. CONCLUSION： Plasma DNA can be a reliable sample source for CIMP analysis. CIMP in plasma may serve as a molecular marker of late-stage and poor-prognosis HCC.

关键词： CpG island methylator phenotype Methylation Plasma Prognosis Hepatocellular carcinoma

A Historical Cohort Study on the Efficacy of Glucocorticoids and Riboflavin Among Patients with Late-onset Multiple Acyi-CoA Dehydrogenase Deficiency

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Chinese Medical Journal 2016年第2期129卷 142-146页

作者： xin-yi liu Zhi-Qiang Wang Dan-Ni Wang Min-Ting Lin Ning Wang Department of Neurology and Institute of Neurology First Affiliated Hospital Fujian Medical University Fuzhou Fujian 350005 China Fujian Key Laboratory of Molecular Neurology Fuzhou Fujian 350005 China

Background： Late-onset multiple acyl-CoA dehydrogenase deficiency （MADD） is the most common type of lipid storage myopathies in China. Most patients with late-onset MADD are well responsive to riboflavin. Up to now, these patients are often treated with glucocorticoids as the first-line drug because they are misdiagnosed as polymyositis without muscle biopsy or gene analysis. Although glucocorticoids seem to improve the fatty acid metabolism of late-onset MADD, the objective evaluation of their rationalization on this disorder and comparison with riboflavin treatment are unknown. Methods： We performed a historical cohort study on the efficacy of the two drugs among 45 patients with late-onset MADD, who were divided into glucocorticoids group and riboflavin group. Detailed clinical information of baseline and 1-month follow-up were collected. Results： After 1-month treatment, a dramatic improvement of muscle strength was found in riboflavin group （P 〈 0.05）. There was no significant difference in muscle enzymes between the two groups. Significantly, the number of patients with full recovery in glucocorticoids group was less than the number in riboflavin group （P 〈 0.05）. On the other hand, almost half of the patients in riboflavin group still presented high-level muscle enzymes and weak muscle strength after 1-month riboflavin treatment, meaning that l-month treatment duration maybe insufficient and patients should keep on riboflavin supplement for a longer time. Conclusions： Our results provide credible evidences that the overall efficacy of riboflavin is superior to glucocorticoids, and a longer duration of riboflavin treatment is necessary for patients with late-onset MADD.

关键词： Glucocorticoids Historical Cohort Study Late-onset Multiple Acyl-CoA Dehydrogenase Deficiency Lipid StorageMyopathy Riboflavin

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韩国经济发展中的对外贸易

中国工业经济 2006年第7期 59-64页

作者：刘信一韩国釜山KOSIN大学

韩国从20世纪60年代试图开始实质性的经济开发,到2004年为止与230多个国家和地区进行贸易。2004年,其GDP是6801亿美元,为世界第10位,人均收入14162美元,交易额4783亿美元,为世界第12位。2004年外币储备额是1991亿美元,居世界第4位。韩... 详细信息

韩国从20世纪60年代试图开始实质性的经济开发,到2004年为止与230多个国家和地区进行贸易。2004年,其GDP是6801亿美元,为世界第10位,人均收入14162美元,交易额4783亿美元,为世界第12位。2004年外币储备额是1991亿美元,居世界第4位。韩国以韩国式经济开发,即实行市场亲和式政府干预的经济政策,通过出口为主导的经济开发实现了经济的快速增长。正高速增长的中国亦以中国式社会主义,以中国式改革开放追求出口和贸易的共同增长。中国和韩国都持续着经济的高速发展,被公认为是以出口和贸易为主的经济开发模式的典范。韩国进口能源、原材料和资本品,出口工业制成品。中国的贸易由外资企业为主进口半成品和资本品,出口工业制成品。而中国贸易在经济发展中所发挥的影响与韩国相比还小。为了持续增长,韩国需要以技术和经营革新提高国际竞争力,尤其要改善以资本品进口为主的贸易结构。

关键词：出口和贸易为主的经济开发政策贸易对经济的影响