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Estimation of Undrained Bearing Capacity for Offshore Soft Foundations with Cyclic Load

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China Ocean Engineering 1998年第2期13卷 213-222页

作者： Wang, JH liu, yf Xing, Y Di, HM Tianjin Univ Civil Sch Geotech Engn Inst Tianjin 300072 Peoples R China Offshore Oil Engn Design Corp China Beijing Peoples R China

The degradation strength of soils under cyclic loading is studied and a method for determining the cyclic degradation strength with cyclic triaxial tests is given in the paper. Furthermore, a dum my static method for estimating the undrained bearing capacity for offshore soft foundation under wave loads is developed. It can consider the effect of the difference of cyclic stress for different parts of the foundation on both the degradation strength of the foundation soil and the bearing capacity so that the estimated result can better reflect the real condition of foundation under cyclic loading. The method can be applied to plane and space problem.

关键词： offshore engineering soft foundation soil dynamics bearing capacity cyclic load

^(99m)Tc-labeled HAb18 McAb Fab fragment for radioimmunoimaging in nude mice bearing human hepatocellular carcinoma

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World Journal of Gastroenterology 1998年第2期4卷 25-28页

作者： Qiu K Wang BC Chen ZN Fang P liu CG Wan WX liu yf State Key Lab. of Nuclear Medicine Inst. of Jiangsu Nuclear Medicine Wuxi 214063 Jiangsu Province China Department of Pathology Fouth Military Medical University Xi'an 710032 Shaanxi Province China Fourth Military Medical University China Department of Pathology Fourth Military Medical University Xi'an 710032 Shaanxi Province 17 West Changle Road China

AIM To establish a method of labeling anti hepatoma McAb (HAb18) Fab fragment modifier with 99m Tc. METHODS HAb18 Fab was modified with 2 iminotholane and labeled with 99m Tc by transchelation from 99m Tc GH. Labeling yield, radiochemical purity and immunoreactivity were determined by thin layer chromatography (TLC SG), paper chromatography (PC), gel chromatography (GC) and cell binding assay, respectively. The nude mice bearing human hepatoma were used for radioimmunoimaging (RII). RESULTS A radiolabeling yield of 50%-80% was obtained, and immunoreactivity (IR) was 30%-40%. Radioimaging results showed that 99m Tc HAb18 McAb Fab fragment was concentrated in the tumor 4-8 hours after injection, and the maximum concentration was seen in 12-24 hours, and the T/NT value was 5 18 and 7 48 at 6h and 8h after the injection. CONCLUSION 99m Tc HAb18 McAb Fab fragment could be specifically localized in the tumor of nude mice bearing human hepatocellular carcinoma within 24 hours and this method might be effectively used for labeling McAb Fab fragment with

关键词： liver neoplasms carcinoma, hepatocellular HAb18 autibodies, monoclonal radioimmunodetection Fab fragments 99m Tc nude mice 99m Tc.

Spectroscopic studies on interaction of hemoglobin and serum albumin with nicotine

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Chinese Science Bulletin 2002年第7期47卷 538-542页

作者： Wang, HF Wang, Y Cheng, Y Sun, HF Wang, XY liu, yf Peking Univ Coll Chem & Mol Engn Dept Appl Chem Beijing 100871 Peoples R China

The interactions of nicotine and Hb/SA were studied in vitro by UV/Vis, fluorescence, 1H NMR and FT-IR spectroscopies. The UV/Vis absorbance of Hb/SA (200nm) shifted to red and decreased gradually with the addition of nicotine, indicating that the protein conformational change resulted from the chemical interaction. With increasing nicotine concentration, incubation of SA with nicotine caused the quenching of fluorescence typical of protein tryptophan residues, which meant that the vicinity of the tryptophan residues of SA was changed because of nicotine. FT-IR spectra showed that α-helix component of Hb/SA decreased, turn and β-structure components of Hb/SA increased in the presence of nicotine. In the 1H NMR spectra of nicotine, all proton peaks on pyrrolidinyl ring moved to downfield and the resonance emanating from nicotine was preferentially broadened while the concentration of Hb/SA increased. All these results indicate that nicotine and Hb/SA in vitro interact on each other, forming a new

关键词： hemoglobin (Hb) serum albumin (SA) nicotine spec-troscopic studies.

Genotoxic effects of low-dose exposure to pirimicarb studied with accelerator mass spectrometry

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Chinese Science Bulletin 1997年第19期42卷 1662-1664页

作者： Shi, JY Wang, HF Wu, XH Li, XS liu, yf Li, K Lu, XY Wang, JJ liu, KX BEIJING UNIV DEPT TECH PHYSBEIJING 100871PEOPLES R CHINA

SINCE 1990 a few papers presented by researchers at the Lawrence Livermore National Labora-tory(LLNL)have shown the great promise of accelerator mass spectrometry(AMS) inthe quantification of biological macromolecular adducts resulting from exposure to the exotictrace chemicals or toxins. For instance, they first successfully detected a carcinogen, 2-amino-3,8-dimethyl-imidazo (4, 5-f) quinoxaline (MeIQx), covalently bound to DNA by AMS.

关键词： pirimicarb DNA adduction accelerator mass spectrometry (AMS).

生物三维打印墨水硬度调控间充质干细胞命运决定的研究

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中华烧伤杂志 2020年第9期36卷 1198-1198页

作者： liu yf Li ZJ Li JJ Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department PLA General Hospital and PLA Medical College 28 Fu Xing Road Beijing 100853 P. R. China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and Regeneration Research Unit of Trauma Care Tissue Repair and Regeneration Chinese Academy of Medical Sciences 2019RU051 51 Fu Cheng Road Beijing 100048 P. R. China

生物三维打印技术的进步为推进组织再生研究提供了巨大潜力。机械性能可调的海藻酸-明胶复合水凝胶是适用于挤压式打印平台的一种常用生物墨水材料,其在长期培养中具有良好的细胞相容性、可印刷性和结构稳定性。大量研究已经证实,水凝... 详细信息

生物三维打印技术的进步为推进组织再生研究提供了巨大潜力。机械性能可调的海藻酸-明胶复合水凝胶是适用于挤压式打印平台的一种常用生物墨水材料,其在长期培养中具有良好的细胞相容性、可印刷性和结构稳定性。大量研究已经证实,水凝胶的生物物理因素对间充质干细胞(MSC)分化起着重要的作用。但是在大多数研究中,基质硬度的变化往往偶联了孔隙及孔隙率的改变;而且MSC在生物三维打印水凝胶中的分化潜能尚未明确。

关键词：生物物理组织再生生物三维打印挤压式细胞相容性水凝胶海藻酸结构稳定性

Association study of NFKB1 and SUMO4 polymorphisms in Chinese patients with psoriasis vulgaris

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Association study of NFKB1 and SUMO4 polymorphisms in Chines...

示指桡侧指背动脉逆行岛状皮瓣移植修复示指缺损

第六次全国中西医结合变态反应学术大会

作者： Hong Li L Gao Z Shen CY Li K Li M Li YJ Lv CX Li TW Gao yf liu Departments:Institute of Dermatology of Chinese PLA Xijing HospitalFourth Military Medical University

Nuclear factor kappa-beta(NF-κB)is a critical transcription factor responsible for modulating the expression of many genes involved in the pathogenesis ***4 is a negative regulator ofNF-κB in the process of cell signaling *** functional single nucleotide polymorphisms(SNP),-94ins/delATTG in NFKB 1gene and the rs237025 A>G in SUMO4 *** been found to be the risk of some autoimmune *** their correlation with the susceptibility of psoriasis vulgaris,a newly-defined autoimmune-related disease,remained unknown *** we genotyped the two polymorphisms in 5 1 9 patients with psoriasis vulgaris and 541 healthy *** found that the ins/ins genotype frequency in NFKB 1 were significant differences among the psoriasis cases and the controls(x2test P=0.01 8).By stratified analysis,the frequency of the ins/ins genotype Was statistically higher among the patients of onset age≤40,PASI>20,male patients and sporadic patients than among *** no statistically significant association has been found between the rs237025A>G polymorphism in SUMO4 gene and the susceptibility ofpsoriasis vulgaris even stratified analysis by the age of onset,family history,PASI score and gender.

关键词： psoriasis NF-κB NFKB1 SUMO4 genetic association SNP

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中国组织工程研究与临床康复 2007年第29期11卷 5668-5671页

作者：谢红炬刘育凤李慧超张继南华大学附属第一医院美容整形科湖南省衡阳市421001 中国医学科学院中国协和医科大学整形外科医院颅颌面一科北京市100041 青岛大学医学院附属医院整形美容科山东省青岛市266012

目的:观察示指桡侧指背动脉逆行岛状皮瓣移植对示指指端缺损的修复效果。方法:选择2002-08/2005-09南华大学附属第一医院急诊收治的外伤性示指末节部分缺损患者13例,手术方法的选择在术前均得到患者同意,且得到医院伦理道德委员会批准... 详细信息

目的:观察示指桡侧指背动脉逆行岛状皮瓣移植对示指指端缺损的修复效果。方法:选择2002-08/2005-09南华大学附属第一医院急诊收治的外伤性示指末节部分缺损患者13例,手术方法的选择在术前均得到患者同意,且得到医院伦理道德委员会批准。组织缺损大小在1.5cm×1.0cm～2.0cm×1.5cm之间,所有缺损均有指骨和(或)大部分的指腹组织缺损,采用示指桡侧指背动脉逆行岛状皮瓣移植修复,皮瓣设计:旋转点位于近侧指间关节的近端,皮瓣轴为示指的桡背侧,皮瓣部分设计在第2掌指关节的近侧,蒂宽约0.8mm。术后定期随访,主要观察皮瓣质地和感觉的恢复情况,将感觉恢复的评估标准分为5级:S1:无感觉;S5:在神经单一分布区恢复两点鉴别能力。结果:13例患者全部进入结果分析,无脱落。①术后随访三四个月者10例,随访五六个月者3例。②术后9例皮瓣完全成活;4例皮瓣远端部分坏死,经换药表皮爬行创面愈合。③外形基本满意,皮瓣色泽、质地良好。④术后1个月随访时,3例患者两点辨别觉大于6.0mm,感觉恢复S4;术后五六个月随访时,3例患者两点辨别觉4.0～6.0mm,感觉恢复S5。结论:示指桡侧指背动脉逆行岛状皮瓣设计的旋转点位于近侧指间关节的近端,可以简化手术而不影响皮瓣存活,是示指部分缺损修复的可选方法。

关键词：指损伤/外科学外科皮瓣组织移植

多普勒超声诊断移植肾排斥反应与病理检查及肾功能指标的对比分析

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中国组织工程研究与临床康复 2007年第38期11卷 7537-7540页

作者：成东华吴刚刘树荣石蕊李桂臣刘永锋中国医科大学附属第一医院器官移植科辽宁省沈阳市110001 中国医科大学附属第一医院器官移植研究所辽宁省沈阳市110001

目的:以病理活检结果为金标准,评估多普勒超声检查对移植肾排斥反应的诊断价值。方法:选择2003-01/2006-12在中国医科大学附属第一医院器官移植科行肾移植并在术后行超声检查的患者176例,均知情同意。①实验分组:根据术后移植肾功能分为... 详细信息

目的:以病理活检结果为金标准,评估多普勒超声检查对移植肾排斥反应的诊断价值。方法:选择2003-01/2006-12在中国医科大学附属第一医院器官移植科行肾移植并在术后行超声检查的患者176例,均知情同意。①实验分组:根据术后移植肾功能分为2组,移植肾功能不良组78例,其中30例次行病理活检;移植肾功能正常组98例。②实验方法及评估:对患者移植肾行多普勒超声检查,参数选择峰收缩期流速、平均舒张期流速、阻力指数及血管显示率。血管显示率的评估标准(0～5级):0级为肾动脉及其远侧血管未显示;5级为肾各级血管均显示良好。以病理活检结果为金标准,分别选取阻力指数=0.7,0.75,0.8,0.85为诊断界值进行诊断试验。结果:169例患者进入结果分析,脱落7例。①峰收缩期流速、平均舒张期流速不呈正态分布,无法作为肾功能评价指标。30例次病理活检中共有28例次被确诊为排斥反应,急性排斥反应15例次,慢性排斥反应13例次。②肾功能正常组患者中血管显示率5级者占63.30%,4级者占36.73%。肾功能不良组患者中血管显示率4级者占41.03%,3级者占46.15%,2级者占10.30%,1级者占2.60%。③移植肾功能不良组患者阻力指数显著高于移植肾功能正常组(P<0.01)。移植肾功能不良组患者移植肾功能恢复后阻力指数显著低于移植肾尚未恢复时(P<0.01),其中99%以上的患者Δ(阻力指数)≥0.20。④界值阻力指数=0.75的诊断试验的敏感性、特异性和准确性最高,均达到100%。结论:当移植肾血管阻力指数升高至0.75以上,特别是同一患者自身对照升高超过0.2以上和或血管显示率低于4级,结合临床表现和生化结果,提示可能出现移植肾排斥反应。

关键词：肾移植超声检查,多普勒,彩色移植物排斥器官移植

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经脾胰岛移植治疗糖尿病大鼠的可行性

中国组织工程研究与临床康复 2007年第16期11卷 3026-3030页

作者：程颖张佳林刘永锋李铁民赵宁中国医科大学附属第一医院器官移植科辽宁省沈阳市110001

目的:探讨经脾胰岛移植治疗糖尿病大鼠的疗效及可行性,建立稳定的胰岛移植新途径。方法:实验于2004-03/2005-03在中国医科大学附属第一医院完成。实验分组:供受鼠均采用Wistar大鼠,供鼠40只,受鼠20只。受鼠随机分为移植组、糖尿病组,每... 详细信息

目的:探讨经脾胰岛移植治疗糖尿病大鼠的疗效及可行性,建立稳定的胰岛移植新途径。方法:实验于2004-03/2005-03在中国医科大学附属第一医院完成。实验分组:供受鼠均采用Wistar大鼠,供鼠40只,受鼠20只。受鼠随机分为移植组、糖尿病组,每组10只。并另取10只正常大鼠作为正常组,进行对照。实验过程:①受鼠造模:移植前14d,采取链尿霉素化学灼伤法(2%链尿霉素,65mg/kg,腹腔注射)造成糖尿病大鼠模型(连续3d在非禁食情况下,血糖>16.8mmol/L为造模成功)。②供鼠胰岛分离纯化:采用明尼苏达大学改良方法,原位直接灌注消化分离大鼠胰岛。消化的胰腺组织离心,经80目不锈钢筛网过滤后,采用25%,23%,20.5%,11%Ficoll和Hank’s液梯度密度离心(4℃,3000r/min×10min),于23%,11%层交界间吸取胰岛细胞。经Hank’s液洗3次,备用。③经脾胰岛移植:移植组大鼠戊巴比妥麻醉后,经左侧肋缘下切口暴露脾脏。显微镜下手检600～800当量胰岛,以1mLCMRL1066培养液稀释,用10mL注射器直接经脾缓慢输注。糖尿病对照组大鼠注射生理盐水1mL。实验评估:①胰岛双硫腙染色与计数,计算纯化率。②体外葡萄糖刺激实验检测胰岛功能。③移植术后监测血糖、血清胰岛素水平。于术后10d行静脉糖耐量实验。④术后14d切取受体大鼠肝脏和脾脏苏木精-伊红染色及Insulin-6免疫组化染色观察组织形态学变化。结果:①每只供鼠可分离300～400当量胰岛,纯度达85%～90%。②体外葡萄糖刺激实验显示胰岛素刺激指数平均为5.59±0.62,功能良好。③受体糖尿病大鼠术后3-5d空腹血糖降至3.4～5.4mmol/L(平均4.8mmol/L)。胰岛素分泌亦达到正常水平。④移植组糖耐量曲线与正常大鼠相似,K值与正常非糖尿病大鼠差异无显著性(2.802,2.3,P>0.05)。⑤受体大鼠脾脏和肝脏内可见散在的移植胰岛。结论:①移植术后3-5d移植胰岛分泌的胰岛素可使空腹血糖达到正常水平。②经脾移植克服了门静脉直接移植引起的出血和门静脉高压的缺点,移植容量可达1mL,可用10mL注射器进行输注,避免反复离心、挤压,可减少胰岛体外损失,是胰岛移植的可行途径。

关键词：胰岛移植糖尿病