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Tomographic flow cytometry by digital holography

Light(Science & Applications) 2016年第1期5卷 128-134页

作者： Francesco Merola Pasquale Memmolo lisa miccio Roberto Savoia Martina Mugnano Angelo Fontana Giuliana D’Ippolito Angela Sardo Achille Iolascon Antonella Gambale Pietro Ferraro CNR-ISASI Istituto di Scienze Applicate e Sistemi Intelligenti‘E.Caianiello’CNR-Consiglio Nazionale delle RicerchePozzuoli 80078Italy CNR-ICB Istituto di Chimica BiomolecolarePozzuoli 80078Italy Department of Molecular Medicine and Medical Biotechnology University of Naples Federico II&CEINGE-Advanced BiotechnologiesNapoli 80145Italy

High-throughput single-cell analysis is a challenging ***-free tomographic phase microscopy is an excellent candidate to perform this ***,in-line tomography is very difficult to implement in practice because it requires a complex set-up for rotating the sample and examining the cell along several *** demonstrate that by exploiting the random rolling of cells while they are flowing along a microfluidic channel,it is possible to obtain in-line phase-contrast tomography,if smart strategies for wavefront analysis are *** fact,surprisingly,a priori knowledge of the three-dimensional position and orientation of rotating cells is no longer needed because this information can be completely retrieved through digital holography wavefront numerical *** approach makes continuous-flow cytotomography suitable for practical operation in real-world,single-cell analysis and with a substantial simplification of the optical system;that is,no mechanical scanning or multi-direction probing is required.A demonstration is given for two completely different classes of biosamples:red blood cells and diatom *** accurate characterization of both types of cells is reported,despite their very different nature and material content,thus showing that the proposed method can be extended by adopting two alternate strategies of wavefront analysis to many classes of cells.

关键词： microfluidics red blood cells tomographic microscopy wavefront analysis

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Digital holography as metrology tool at micronanoscale for soft matter

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Light(Advanced Manufacturing) 2022年第1期3卷 128-153页

作者： Zhe Wang lisa miccio Sara Coppola Vittorio Bianco Pasquale Memmolo Volodymyr Tkachenko Vincenzo Ferraro Ernesto Di Maio Pier Luca Maffettone Pietro Ferraro CNR-ISASI Institute of Applied Sciences and Intelligent Systems"E.Caianiello"Via Campi Flegrei 3480078 PozzuoliNapoliItaly Dipartimento di Ingegneria Chimica dei Materiali e della Produzione Industriale Universitàdegli Studi di Napoli"Federico II"Piazzale Tecchio 8080125 NapoliItaly NEAPoLIS Numerical and Experimental Advanced Program on Liquids and Interface SystemsJoint Research Center CNR Universitàdegli Studi di Napoli"Federico II"NapoliItaly

The appearance of the first laser approximately 12 years after the invention of holography by Gabor(1948)revolutionized the field of optical *** fact,the invention of holographic interferometry enabled the exploitation of interferometry on non-mirror surfaces and full-scale *** holography-based measurement methods has been implemented to several industrial systems or in support of R&D with the aim of improving new products in many fields(automotive,aerospace,electronics,etc.).To date,holography has been considered an important measurement tool for non-destructive inspection(NDI),strain-stress measurement,and vibration analysis at various engineering ***,the new paradigm of Industry4.0 has seen the introduction of new technologies and methods of processing materials as well as the development of manufacturing approaches for the realization of innovative *** example,direct printing,additive,and bottom-up manufacturing processes are expected to involve new ways of making products in future,and most innovative fabrication processes will be based on the manipulation of soft matter(e.g.,starting from the liquid phase)that will be shaped at the *** inherent characteristics of digital holography(DH)make it a powerful and accurate tool for the visualization and testing of final products,as well as for in situ and real-time monitoring and quantitative characterization of the processes involved during the fabrication *** review aims to report on the most useful applications of soft matter,where the capabilities offered by DH,such as three-dimensional(3D)imaging,extended focus,3D tracking,full-field analysis,high sensitivity,and a wide range of measurements from nanometers to centimeters,permit completely non-invasive characterizations on a *** holographic experimental results of typical samples are reported and discussed where DH plays a primary role as a tool gauge for soft matter.

关键词： Digital holography Soft matter Microscopy Thin film Polymers Liquids Characterization Interferometry Inkjet printing

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3D imaging lipidometry in single cell by in-flow holographic tomography

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Opto-Electronic Advances 2023年第1期6卷 10-25页

作者： Daniele Pirone Daniele Sirico lisa miccio Vittorio Bianco Martina Mugnano Danila del Giudice Gianandrea Pasquinelli Sabrina Valente Silvia Lemma Luisa Iommarini Ivana Kurelac Pasquale Memmolo Pietro Ferraro CNR-ISASI Institute of Applied Sciences and Intelligent Systems“E.Caianiello”Via Campi Flegrei 34PozzuoliNapoli 80078Italy Department of Electrical Engineering and Information Technologies University of Naples“FedericoⅡ”via Claudio 21Napoli 80125Italy Department of Chemical Materials and Production Engineering of the University of Naples FedericoⅡPiazzale Tecchio 80Napoli 80125Italy Department of Mathematics and Physics University of Campania“Luigi Vanvitelli”Caserta 81100Italy Biotechnology and Methods in Laboratory Medicine Department of ExperimentalDiagnostic and Specialty Medicine(DIMES)University of BolognaBologna 40126Italy Subcellular Nephro-Vascular Diagnostic Program Pathology Unit S.Orsola IRCCSUniversity of BolognaBologna 40126Italy Unit of Medical Genetics Department of Medical and Surgical Sciences(DIMEC)University of BolognaVia Massarenti 9Bologna 40138Italy Study and Research Center on Gynecological Neoplasias Department of Medical and Surgical Sciences(DIMEC)University of BolognaVia Massarenti 9Bologna 40138Italy Center for Applied Biomedical Research(CRBA) University of BolognaBologna 40138Italy Department of Pharmacy and Biotechnology(FABIT) University of BolognaBologna 40126Italy

The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets(LDs)in several regulatory mechanisms in living *** are dynamic organelles and therefore their complete characterization in terms of number,size,spatial positioning and relative distribution in the cell volume can shed light on the roles played by *** now,fluorescence microscopy and transmission electron microscopy are assessed as the gold standard methods for identifying LDs due to their high sensitivity and ***,such methods generally only provide 2D assays and partial ***,both can be destructive and with low productivity,thus limiting analysis of large cell numbers in a *** we demonstrate for the first time the capability of 3D visualization and the full LD characterization in high-throughput with a tomographic phase-contrast flow-cytometer,by using ovarian cancer cells and monocyte cell lines as models.A strategy for retrieving significant parameters on spatial correlations and LD 3D positioning inside each cell volume is *** information gathered by this new method could allow more in depth understanding and lead to new discoveries on how LDs are correlated to cellular functions.

关键词： lipid droplets label-free phase-contrast imaging in-flow tomography 3D imaging

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Risk Analysis of Historic Urban Areas： A Case Study of the Salerno City, Italy

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Journal of Civil Engineering and Architecture 2015年第9期9卷 1099-1106页

作者： Federica Ribera Luigi Petti Gennaro miccio Alessandra Landi Alessio Lodato Department of Civil Engineering University of Salerno Fisciano 84084 Italy Ministry of Cultural Heritage and Activities and Tourism Roma 00186 Italy

The preservation of historical and cultural heritage from seismic risk is a question of interest in Italy. However, too little has been done to deal with this problem. First of all, the seismic safety of cultural heritage should be a topic of prevention. The research described in the paper presents a multi-approach procedure to address risk assessment of large-scale urban systems with the aim to contribute at the development of risk mitigation policies for historical centers buildings. The observation of the earthquakes effects in Italy clearly highlights the role of the high vulnerability of the existing building on the consequences in terms of casualties and damage, rather than the severity of seismic events, especially in historical contexts. Unlike the new buildings which should be planned and designed to respect seismic safety requirements, existing buildings require a careful safety assessment on the basis of their susceptibility to damage which depends on building techniques, past damage events, maintenance, changes, etc.

关键词： Seismic hazard historical centers vulnerability

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