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Assessing bioactivity of environmental water samples filtered using nanomembrane technology and mammalian cell lines

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Eco-Environment & Health 2024年第3期3卷 347-354页

作者： Sarah E.Morgan lisa a.delouise Department of Environmental Medicine University of Rochester Medical CenterRochesterNY 14642USA Department of Biomedical Engineering University of RochesterRochesterNY 14627USA Department of Dermatology University of Rochester Medical CenterRochesterNY 14642USA Lake Ontario Center for Microplastics and Human Health in a Changing Environment University of Rochester Medical CenterRochesterNY 14642USA

This project reports on the use of a novel nanomembrane filtering technology to isolate and analyze the bioactivity of microplastic(MP)-containing debris from Lake Ontario water *** MPs are a complex mixture of polymers and sorbed chemicals that are persistent and can exhibit a wide range of toxic *** human exposure to MPs is unavoidable,it is necessary to characterize their bioactivity to assess potential health *** work seeks to quantify MP presence in the nearshore waters of Lake Ontario and begin to characterize the bioactivity of the filtrate containing *** utilized silicon nitride(SiN)nanomembrane technology to isolate debris sized between 8 and 20μm from lake water samples collected at various times and *** were identified with Nile red ***-based assays were conducted directly on the filtered debris to test for cell viability,aryl hydrocarbon receptor(AhR)activity,and interleukin 6(IL-6)levels as a measure of proinflammatory *** samples contained *** of the isolated debris impacted cell ***,AhR activity and IL-6 levels varied over ***,no associations were observed between the amount of plastic and *** differences in activity are likely due to variations in the physiochemical properties of debris between *** results highlight the need for increased sampling to fully characterize the bioactivity of MPs in human cells and to elucidate the role that sample physiochemical and spatiotemporal properties play in this activity.

关键词： Microplastics SiN nanomembranes Lake Ontario Aryl hydrocarbon receptor(AhR)activity IL-6 levels Bioactivity

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Microfabrication of Bubbular Cavities in PDMS for Cell Sorting and Microcell Culture Applications

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Journal of Bionic Engineering 2008年第4期5卷 308-316页

作者： Ut-Binh T. Giang Michael R. King lisa A. delouise Department of Biomedical Engineering University of Rochester Department of Biomedical Engineering Cornell University Department of Dermatology University of Rochester Medical Center

We describe a novel technique, low surface energy Gas Expansion Molding （GEM）, to fabricate microbubble arrays in polydimethylsiloxane （PDMS） which are incorporated into parallel plate flow chambers and tested in cell sorting and microcell cuTture applications. This architecture confers several operational advantages that distinguish this technology approach from currently used methods. Herein we describe the GEM process and the parameters that are used to control microbubble formation and a Vacuum-Assisted Coating （VAC） process developed to selectively and spatially alter the PDMS surface chemistry in the wells and on the microchannel surface. We describe results from microflow image visualization studies conducted to investigate fluid streams above and within microbubble wells and conclude with a discussion of cell culture studies in PDMS.

关键词： polydimethylsiloxane microfabrication cell culture cell sorting molding

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