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Quantitative assessment of the breast cancer marker HER2 using a gold nanoparticle-based lateral flow immunoassay

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Nano Research 2024年第6期17卷 5452-5460页

作者： Liya Ye Xinxin Xu Aihua Qu liqiang liu Chuanlai Xu Hua Kuang International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Human epidermal growth factor receptor 2(HER2)is an important biomarker for detection and treatment of breast *** this study,we developed monoclonal antibodies against the extracellular domain(ECD)of HER2 and established a rapid and accurate lateral flow immunoassay(LFIA)for use in community medical *** gene sequence of human HER2-ECD was obtained from the National Center for Biotechnology Information(NCBI)to construct the expression ***2-ECD protein expressed in HEK293F cells was used to immunize BALB/c *** monoclonal antibodies were produced in mouse ascites and isolated by hybridoma cell *** were analyzed for purity by SDS-PAGE(sodium dodecyl sulphate-polyacrylamide gel-electrophoresis)and affinity was assessed by enzyme-linked immunosorbent assay(ELISA)while subtypes were detected using the commercial *** HER2-ECD test strip was prepared based on the sandwich method and evaluated using a portable detection *** affinity of the paired antibodies,4D8 and 8D9,both reached 1×108 L/*** antibodies specifically recognized the HER2-ECD protein in *** limit of detection(LOD)of the gold nanoparticle(AuNP)-based LFIA was 1.7 ng/mL with a detection range of 1.7-400 ng/mL,and the performance of the HER2-ECD strip correlated well with that of a Siemens chemiluminescent immunoassay(CLIA)*** conclusion,the paired antibodies were successfully prepared with high affinity and *** AuNP-based LFIA of HER2-ECD provides a fast and accurate method to detect the concentration of HER2-ECD in serum samples for clinical use in community medical institutions,and could contribute to determining the progress of the disease or the effectiveness of treatment.

关键词： human epidermal growth factor receptor 2 breast cancer monoclonal antibody quantitative lateral flow immunoassay

A gold-based multiplex immunochromatographic sensor for detecting paclitaxel

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Nano Research 2024年第7期17卷 6420-6429页

作者： Xiaoqian Jiang Aihua Qu Xinxin Xu Hua Kuang liqiang liu Chuanlai Xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Paclitaxel(PTX),methotrexate(MTX),and 5-fluorouracil(5-FU)are commonly-used small molecule anti-tumor drugs for breast ***,drug resistance occurs with long-term treatment or excessive use,and the high concentrations of PTX,MTX,and 5-FU in patients may lead to side effects with dose-limiting toxicities,such as myelosuppression,hepatotoxicity,and peripheral ***,concentration monitoring of PTX,MTX,and 5-FU in clinical treatment is very *** prepared highly sensitive and specific monoclonal antibodies using several haptens,and established a multiple paper-based sensor utilizing optical signals of gold nanoparticles,which simultaneously detected PTX,MTX,and 5-FU in human plasma or urine with 10 min.A portable scanner for quantitative detection in plasma and urine samples was used,and the calculated limit of detection(cLOD)values were 0.002 and 0.142μg/mL for PTX,0.187 and 0.976 ng/mL for MTX,and 0.057 and 0.128μg/mL for 5-FU,*** the recovery test,the recovery rates and the coefficient of variation were 85.84%–108.81%and 1.23%–8.80%,respectively,indicating the reliability and accuracy of the multiple gold immunochromatographic strip(GIS).In addition,for the determination of collected clinical plasma samples,the correlation between the test data of the multiple GIS and liquid chromatography–tandem mass spectrometry(LC–MS/MS)was ***,the developed multiple GIS could be applied to the rapid detection of PTX,MTX,and 5-FU in different clinical samples.

关键词： paclitaxel methotrexate 5-fluorouracil immunochromatographic assay plasma urine

Intense and Superflat White Laser with 700-nm 3-dB Bandwidth and 1-mJ Pulse Energy Enabling Single-Shot Subpicosecond Pulse Laser Spectroscopy

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Research 2024年第2期6卷 319-327页

作者： Lihong Hong Haiyao Yang liqiang liu Mingzhou Li Yuanyuan liu Baoqin Chen Huakang Yu Wenbo Ju Zhi-Yuan Li School of Physics and Optoelectronics South China University of TechnologyGuangzhou 510641China State Key Laboratory of Luminescent Materials and Devices South China University of TechnologyGuangzhou 510640China

An optical spectrometer is a basic spectral instrument that probes microscopic physical and chemical properties of macroscopic objects but generally suffers from difficulty in broadband time-resolved *** this work,we report the creation of ultrabroadband white-light laser with a 3-dB bandwidth covering 385 to 1,080 nm,pulse energy of 1.07 mJ,and pulse duration of several hundred femtoseconds by passing 3-mJ pulse energy,50-fs pulse duration Ti:Sapphire pulse laser through a cascaded fused silica plate and chirped periodically poled lithium niobate *** utilize this unprecedented superflat,ultrabroadband,and intense femtosecond laser light source to build a single-shot(i.e.,single-pulse)subpicosecond pulse laser ultraviolet-visible-near-infrared spectrometer and successfully measure various atomic and molecular absorption *** single-shot ultrafast spectrometer may open up a frontier to monitor simultaneously the ultrafast dynamics of multiple physical and chemical processes in various microscopic systems.

关键词： Pulse utilize frontier

A multi-colloidal gold immunochromatography assay for rapid and simultaneous detection of 13 herbicides

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Nano Research 2024年第9期17卷 8368-8376页

作者： Qianqian Lu Xinxin Xu Aihua Qu liqiang liu Yuzhe Li Maozhong Sun Chuanlai Xu Hua Kuang International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China China National Center for Food Safety Risk Assessment Beijing 100021China

Herbicide residues in agricultural products can have adverse effects on the environment and human health,therefore,there is an urgent need to establish a sensitive,rapid,and wide-ranging detection *** this study,haptens of phenylurea herbicides(PUs)and sulfonylurea herbicides(SUs)were analyzed and designed based on computational simulation techniques,and two high-performance broad-spectrum monoclonal antibodies against PUs and SUs were *** this basis,a multi-colloidal gold immunochromatography assay(multi-CGIA)was developed to simultaneously detect 13 herbicides in *** visual limit of detection(vLOD)for PUs including diuron,chlortoluron,neburon,chlorbromuron,and linuron was 1-2μg/*** vLOD for SUs including metsulfuron methyl,ethametsulfuron-methyl,sulfometuron-methyl,tribenuron methyl,cinosulfuron,triasulfuron,chlorimuron-ethyl,and chlorsulfuron was 2-10μg/*** results of real sample determination indicated that the multi-CGIA is accurate,stable,and reliable,and adaptable to on-site preliminary screening of actual samples.

关键词： phenylurea herbicides sulfonylurea herbicides monoclonal antibody immunochromatographic assay wheat

A global model of intensity autocorrelation to determine laser pulse duration

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Chinese Physics B 2024年第5期33卷 446-451页

作者：彭雨菲刘励强洪丽红李志远 School of Physics and Optoelectronics South China University of TechnologyGuangzhou 510640China

We present a new global model of collinear autocorrelation based on second harmonic generation *** model is rigorously derived from the nonlinear coupled wave equation specific to the autocorrelation measurement configuration,without requiring a specific form of the incident pulse function.A rigorous solution of the nonlinear coupled wave equation is obtained in the time domain and expressed in a general analytical *** global model fully accounts for the nonlinear interaction and propagation effects within nonlinear crystals,which are not captured by the classical local *** assess the performance of the global model compared to the classic local model,we investigate the autocorrelation signals obtained from both models for different incident pulse waveforms and different full-widthes at half-maximum(FWHMs).When the incident pulse waveform is Lorentzian with an FWHM of 200 fs,the global model predicts an autocorrelation signal FWHM of 399.9 fs,while the classic local model predicts an FWHM of 331.4 *** difference between the two models is 68.6 fs,corresponding to an error of 17.2%.Similarly,for a sech-type incident pulse with an FWHM of 200 fs,the global model predicts an autocorrelation signal FWHM of 343.9 fs,while the local model predicts an FWHM of 308.8 *** difference between the two models is 35.1 fs,with an error of 10.2%.We further examine the behavior of the models for Lorentzian pulses with FWHMs of 100 fs,200 fs and 500 *** differences between the global and local models are 17.1 fs,68.6 fs and 86.0 fs,respectively,with errors approximately around 17%.These comparative analyses clearly demonstrate the superior accuracy of the global model in intensity autocorrelation modeling.

关键词： intensity autocorrelation global model ultrashort pulses pulse-width measurement

Preliminary research and scheme design of deep underground in situ geo-information detection experiment for Geology in Time

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International Journal of Mining Science and Technology 2024年第1期34卷 1-13页

作者： Heping Xie Ru Zhang Zetian Zhang Yinshuang Ai Jianhui Deng Yun Chen Yong Zhou Mingchuan Li liqiang liu Mingzhong Gao Zeqian Yang Weiqiang Ling Heng Gao Qijun Hao Kun Xiao Chendi Lou State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering Sichuan UniversityChengdu 610065China College of Water Resource&Hydropower Sichuan UniversityChengdu 610065China Guangdong Provincial Key Laboratory of Deep Earth Sciences and Geothermal Energy Exploitation and Utilization Institute of Deep Earth Sciences and Green EnergyShenzhen UniversityShenzhen 518060China Institute of Geology and Geophysics Chinese Academy of SciencesBeijing 100029China Yalong River Hydropower Development Company Limited Chengdu 610051China

The deep earth,deep sea,and deep space are the main parts of the national“three deep”strategy,which is in the forefront of the strategic deployment clearly defined in China’s 14th Five-Year Plan(2021-2025)and the Long-Range Objectives Through the Year *** is important to reveal the evolutionary process and mechanism of deep tectonics to understand the earth’s past,present and *** academic con-notation of Geology in Time has been given for the first time,which refers to the multi-field evolution response process of geological bodies at different time and spatial scales caused by geological processes inside and outside the *** on the deep in situ detection space and the unique geological envi-ronment of China Jinping Underground Laboratory,the scientific issue of the correlation mechanism and law between deep internal time-varying and shallow geological response is given *** research and frontier exploration on deep underground in situ geo-information detection experiments for Geology in Time are designed to be carried out,which will have the potential to explore the driving force of Geology in Time,reveal essential laws of deep earth science,and explore innovative technologies in deep underground engineering.

关键词： Deep underground Geology in Time China Jinping Underground Laboratory In situ detection

人体不同部位和个体差异对脂肪组织及其间充质干细胞的影响

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中华整形外科杂志 2024年第8期40卷 917-923页

作者：岳强刘立强中国医学科学院北京协和医学院整形外科医院会阴整形与性别重塑科北京100144

脂肪组织不仅是重要的内分泌器官,也是一种区域性免疫器官,其内的脂肪源性间充质干细胞(ADSCs)是近年来再生医学和脂肪移植领域的热点。然而区域免疫稳态及代谢的失衡会通过代谢重编程等机制引起脂肪组织功能变化,使不同部位和个体来源... 详细信息

脂肪组织不仅是重要的内分泌器官,也是一种区域性免疫器官,其内的脂肪源性间充质干细胞(ADSCs)是近年来再生医学和脂肪移植领域的热点。然而区域免疫稳态及代谢的失衡会通过代谢重编程等机制引起脂肪组织功能变化,使不同部位和个体来源的脂肪细胞存在差异。部位和个体因素对脂肪组织及其间充质干细胞的影响一直是整形外科医生关注的热点,但这一领域仍存在较大争议,限制了脂肪和ADSCs的临床应用。该文对比了人体不同部位脂肪组织差异,并分析了不同个体因素对脂肪组织功能的影响、可能原因和代谢重编程机制,为更好地探究脂肪代谢功能和ADSCs的临床应用提供参考。

关键词：脂肪组织脂肪源性间充质干细胞肥胖细胞功能代谢重编程

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氦气内纯化器分析与实验

华中科技大学学报（自然科学版） 2024年第7期52卷 132-138页

作者：杜军军李正宇刘立强龚领会中国科学院理化技术研究所北京100190 中国科学院大学北京100049

在不增加冷源氦气消耗前提下以提高氦气纯化器的污氦气处理能力为目标,分析了氦气内纯化的工作过程.优化了氦气内纯化器新流程,利用液化杂质冷量可有效降低冷源氦气消耗量,有利于扩展内纯化器低纯度氦的处理范围.设计了一台氦气内纯化... 详细信息

在不增加冷源氦气消耗前提下以提高氦气纯化器的污氦气处理能力为目标,分析了氦气内纯化的工作过程.优化了氦气内纯化器新流程,利用液化杂质冷量可有效降低冷源氦气消耗量,有利于扩展内纯化器低纯度氦的处理范围.设计了一台氦气内纯化器并开展了相关实验,结果表明:新内纯化器可将氦气纯度约为92.0%的污氦气提纯至99.999%高纯氦气;相较于现有商用氦气内纯化器,优化后内纯化器流程在不降低冷源氦气消耗的前提下,使得污氦气处理范围由氦气纯度95.0%扩展到92.0%.当高纯氦气产量不低于180 L/min时,冷源消耗量约32 L/min,冷源氦气消耗量降低约54.3%,新的内纯化器流程有利于提高为内纯化器提供冷量的氦液化器的液化量.

关键词：氦气内纯化器纯化分离流程分析优化设计

啁啾周期极化铌酸锂晶体在1064 nm倍频实验中的效率与温度鲁棒性

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光学学报 2024年第11期44卷 187-195页

作者：苏伟伦刘峻铭邹娱刘励强洪丽红李志远华南理工大学物理与光电学院广东广州510641 广东晶创光学机械有限公司广东广州510510 中国科学院上海光学精密机械研究所强场激光物理国家重点实验室上海201800

为了提升532 nm激光的产生效率和功率稳定性,以基于倒格矢实现准相位匹配的手段为基础,设计了合适的啁啾周期极化铌酸锂(CPPLN)晶体的结构并进行仿真,采用自制的1064 nm激光器对该结构的晶体进行腔外倍频实验。在输入的1064 nm连续光功... 详细信息

为了提升532 nm激光的产生效率和功率稳定性,以基于倒格矢实现准相位匹配的手段为基础,设计了合适的啁啾周期极化铌酸锂(CPPLN)晶体的结构并进行仿真,采用自制的1064 nm激光器对该结构的晶体进行腔外倍频实验。在输入的1064 nm连续光功率为22.53 W的条件下可以获得功率为148 mW、波长为532 nm的光,光光转换效率约为0.66%,是三硼酸锂(LBO)的15.58倍。进一步验证了CPPLN晶体的温度鲁棒性,532 nm倍频光的功率关于温度的半峰全宽为8.40℃,且在该范围内功率呈现振荡的趋势,其原因是加工精度的限制导致倒格矢分布不连续。所得到的输出倍频光的光斑为标准的高斯光斑,其并不受温度的影响。结果表明,该设计结构的CPPLN晶体相对于传统LBO晶体具有更高的倍频效率与温度鲁棒性。

关键词：非线性光学啁啾周期极化铌酸锂晶体温度鲁棒性腔外倍频