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中华医学会第十七次全国儿科学术大会

作者： liping yuan,ling lu,qin zhang,bo hu department of pediatrics,first affiliated hospital of anhui medical university,hefei,china

Aim HSP is a common immunoglobulin A(IgA),especially lgA1-mediated vasculitis in *** has been showed that apoptosis of vascular endothelial cells played an important role in the pathogenesis of *** this study,we investigated the apoptosis of vein endothelial cells in HSP patients. Methods lgA1 was isolated from sera at the acute stage of childhood HSP and the apoptosis rate of human umbilical vein endothelial cells(huVEC) induced by lgA1 was analyzed using flow cytometry and TUNEL ***-2,Bax and P53 mRNA and protein expression was determined by Real-time quantitative RT-PCR and western blotting methods. Results The results showed that isolated lgA1 markedly induced the apoptosis death of huVEC compared with normal control *** the bax and P53 expression was up-regulated and the Bcl-2 expression was down-regulated in huVEC co-cultured with lgA1 isolated from HSP patients for 24h. Conclusion These findings suggested that lgA1 isolated from HSP patients had an effect on the apoptosis of huVEC;moreover bax/bcl-2 and p53 signaling pathways played a key role in the process of apoptosis in huVEC induced by lgA1.

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IgA1 from HSP Patients Trigger Apoptosis and Inhibit Cytoskeletal Proteins in huVEC

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Open Journal of pediatrics 2014年第1期4卷 42-46页

作者： liping yuan Wenjun Fei Lin Wu Ming Gui qin zhang bo hu department of pediatrics First Affiliated Hospital of Anhui Medical UniversityHefeiChina department of medical Technology Anhui Medical CollegeHefeiChina

Background: Henoch-Schonlein purpura (HSP) is a kind of systemic small vessel vasculitis in children. Endothelium cells injury induced by IgA1 is considered important in the pathogenesis of HSP. Research found that the apoptosis of vein endothelial cells was related to the vasculitis in HSP patients. Purpose: To observe the effect of IgA1 from HSP patients on the apoptosis of huVEC and firstly analyze the mechanism of the apoptosis of huVEC induced by IgA1. Methods: huVECs were cultured in 3 different conditional media with IgA1 from HSP patients, normal healthy children and simply medium (blank control). Serum IgA1 was purified by jacalin affinity chromatography. The rates of apoptosis in huVEC incubated with IgA1 were determined by TUNEL method and flow cytometry, respectively. The expression of the cytoskeletal proteins, such as FAK, Vinculin and MLCK was detected with the methods of Real-time PCR and Westernblot, respectively. Results: The present study showed that the apoptosis rate of huVEC by IgA1 isolated from HSP patients was higher than blank control (14.77% ± 2.23% vs 2.25% ± 0.77%) (P huVEC by IgA1 from normal healthy children was higher than blank control (9.97% ± 1.48% vs 2.25% ± 0.77%) (P huVEC co-cultured with IgA1 isolated from HSP patients for 24h. Conclusion: These findings firstly on IgA1 from HSP patients may induce apoptosis of vascular endothelial cells through inhibiting the cytoskeletal proteins expression. IgA1 may accelerate progression of HSP by inducing apoptosis of vascular endothelial cells.

关键词： Henoch-Schonlein Purpura(HSP) Endothelial Cells Immunoglobulin A Apoptosis Cytoskeletal Proteins

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