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How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models

World Journal of Stem Cells 2022年第8期14卷 658-679页

作者： Murilo Montenegro Garrigós Fernando Anselmo de Oliveira Mariana Penteado Nucci Leopoldo Penteado Nucci Arielly da Hora Alves Olivia Furiama Metropolo Dias lionel fernel gamarra Hospital Israelita Albert Einstein São Paulo 05652-900São PauloBrazil Instituto de Química Universidade de São PauloSão Paulo 05508-900São PauloBrazil LIM44-Hospital das Clínicas Faculdade Medicina da Universidade de São PauloSão Paulo 05403-000Brazil Centro Universitário do Planalto Central Área Especial para Industria nº02 Setor Leste-Gama-DFBrasília 72445-020Distrito FederalBrazil

BACKGROUND Bone marrow transplantation(BMT)can be applied to both hematopoietic and nonhematopoietic diseases;nonetheless,it still comes with a number of challenges and limitations that contribute to treatment *** this in mind,a possible way to increase the success rate of BMT would be cotransplantation of mesenchymal stem cells(MSCs)and hematopoietic stem cells(HSCs)to improve the bone marrow niche and secrete molecules that enhance the hematopoietic *** To analyze HSC and MSC characteristics and their interactions through cotransplantation in murine *** We searched for original articles indexed in PubMed and Scopus during the last decade that used HSC and MSC cotransplantation and in vivo BMT in animal models while evaluating cell *** excluded in vitro studies or studies that involved graft versus host disease or other hematological diseases and publications in languages other than *** PubMed,we initially identified 555 articles and after selection,only 12 were *** Scopus,2010 were identified,and six were left after the screening and eligibility *** Of the 2565 articles found in the databases,only 18 original studies met the eligibility *** distribution by source showed similar ratios,with human umbilical cord blood or animal bone marrow being administered mainly with a dose of 1×10^(7) cells by intravenous or intrabone ***,MSCs had a high prevalence of human donors with a variety of sources(umbilical cord blood,bone marrow,tonsil,adipose tissue or fetal lung),using a lower dose,mainly 106 cells and ranging 104 to 1.5×107 cells,utilizing the same *** were characterized prior to administration in almost every *** recipient used was mostly immunodeficient mice submitted to low-dose irradiation or *** main technique of engraftment for HSC and MSC cotransplantation evaluation was chimerism,followed by hematopoietic reconstitution and survival anal

关键词： Mesenchymal stem cells Hematopoietic stem cells Bone marrow transplantation Cotransplantation Hematopoietic reconstitution Engraftment

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Current overview of induced pluripotent stem cell-based blood-brain barrier-on-a-chip

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World Journal of Stem Cells 2023年第6期15卷 632-651页

作者： Arielly da Hora Alves Mariana Penteado Nucci Nicole Mastandrea Ennes do Valle Juliana Morais Missina Javier Bustamante Mamani Gabriel Nery Albuquerque Rego Olivia Furiama Metropolo Dias Murilo Montenegro Garrigós Fernando Anselmo de Oliveira lionel fernel gamarra Hospital Israelita Albert Einstein São Paulo 05529-060São PauloBrazil Hospital das Clínicas HCFMUSP Faculdade de MedicinaUniversidade de São PauloSão Paulo 05403-010São PauloBrazil

BACKGROUND Induced pluripotent stem cells(iPSCs)show great ability to differentiate into any tissue,making them attractive candidates for pathophysiological *** rise of organ-on-a-chip technology in the past century has introduced a novel way to make in vitro cell cultures that more closely resemble their in vivo environments,both structural and *** literature still lacks consensus on the best conditions to mimic the blood-brain barrier(BBB)for drug screening and other personalized *** development of models based on BBB-on-achip using iPSCs is promising and is a potential alternative to the use of animals in *** To analyze the literature for BBB models on-a-chip involving iPSCs,describe the microdevices,the BBB in vitro construction,and *** We searched for original articles indexed in PubMed and Scopus that used iPSCs to mimic the BBB and its microenvironment in microfluidic *** articles were identified,wherein only 14 articles were finally selected according to the inclusion and exclusion *** compiled from the selected articles were organized into four topics:(1)Microfluidic devices design and fabrication;(2)characteristics of the iPSCs used in the BBB model and their differentiation conditions;(3)BBB-on-a-chip reconstruction process;and(4)applications of BBB microfluidic three-dimensional models using *** This study showed that BBB models with iPSCs in microdevices are quite novel in scientific *** technological advances in this area regarding the use of commercial BBB-on-a-chip were identified in the most recent articles by different research *** polydimethylsiloxane was the most used material to fabricate in-house chips(57%),whereas few studies(14.3%)adopted *** the models were constructed using a porous membrane made of diverse materials to separate the *** sources were divergent among the studies,but th

关键词： Induced pluripotent stem cells Cell differentiation Blood-brain barrier Neurovascular unit Organ-on-a-chip Microfluidic device

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Stem cell homing, tracking and therapeutic efficiency evaluation for stroke treatment using nanoparticles: A systematic review

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World Journal of Stem Cells 2020年第5期12卷 381-405页

作者： Mariana Penteado Nucci Igor Salerno Filgueiras Joao Matias Ferreira Fernando Anselmo de Oliveira Leopoldo Penteado Nucci Javier Bustamante Mamani Gabriel Nery Albuquerque Rego lionel fernel gamarra LIM44 Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao Paulo 05529-060Brazil Hospital Israelita Albert Einstein Sao Paulo 05529-060Brazil Centro Universitário do Planalto Central BrasíliaDFBrazil

BACKGROUND Stroke is the second leading cause of death *** is a real need to develop treatment strategies for reducing neurological deficits in stroke survivors,and stem cell(SC)therapeutics appear to be a promising alternative for stroke therapy that can be used in combination with approved thrombolytic or thrombectomy ***,the efficacy of SC therapy depends on the SC homing ability and engraftment into the injury site over a long period of ***,tracking SCs from their niche to the target tissues is a complex *** To evaluate SC migration homing,tracking and therapeutic efficacy in the treatment of stroke using nanoparticles METHODS A systematic literature search was performed to identify articles published prior to November 2019 that were indexed in PubMed and *** following inclusion criteria were used:(1)Studies that used in vivo models of stroke or ischemic brain lesions;(2)Studies of SCs labeled with some type of contrast agent for cell migration detection;and(3)Studies that involved in vivo cellular homing and tracking *** A total of 82 articles were identified by indexing in Scopus and Pub *** inclusion criteria were applied,35 studies were selected,and the articles were assessed for eligibility;ultimately,only 25 studies were *** of the selected studies used SCs from human and mouse bone marrow labeled with magnetic nanoparticles alone or combined with fluorophore *** cells were administered in the stroke model(to treat middle cerebral artery occlusion in 74%of studies and for photothrombotic induction in 26%of studies).Fiftythree percent of studies used xenogeneic grafts for cell therapy,and the migration homing and tracking evaluation was performed by magnetic resonance imaging as well as other techniques,such as near-infrared fluorescence imaging(12%)or bioluminescence assays(12%).CONCLUSION Our systematic review provided an up-to-date evaluation of SC migration hom

关键词： Stem cell Nanoparticles Homing Tracking Near-infrared fluorescence image Cellular therapy Magnetic resonance image Bioluminescence Stroke

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Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

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World Journal of Stem Cells 2019年第2期11卷 100-123页

作者： Helio Rodrigues da Silva Javier Bustamante Mamani Mariana Penteado Nucci Leopoldo Penteado Nucci Andrea Tiemi Kondo Daianne Maciely Carvalho Fantacini Lucas Eduardo Botelho de Souza Virginia Picanço-Castro Dimas Tadeu Covas José Mauro Kutner Fernando Anselmo de Oliveira Nelson Hamerschlak lionel fernel gamarra Hospital Israelita Albert Einstein LIM44 Hospital das Clinicas HCFMUSP Faculdade de MedicinaUniversidade de São Paulo Centro Universitário do Planalto Central Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo

BACKGROUND Mesenchymal stem cells(MSCs) have been widely tested for their therapeutic efficacy in the ischemic brain and have been shown to provide several benefits. A major obstacle to the clinical translation of these therapies has been the inability to noninvasively monitor the best route, cell doses, and collateral effects while ensuring the survival and effective biological functioning of the transplanted stem cells. Technological advances in multimodal imaging have allowed in vivo monitoring of the biodistribution and viability of transplanted stem cells due to a combination of imaging technologies associated with multimodal nanoparticles(MNPs) using new labels and covers to achieve low toxicity and longtime residence in *** To evaluate the sensitivity of triple-modal imaging of stem cells labeled with MNPs and applied in a stroke *** After the isolation and immunophenotypic characterization of human bonemarrow MSCs(hBM-MSCs), our team carried out lentiviral transduction of these cells for the evaluation of bioluminescent images(BLIs) in vitro and in vivo. In addition, MNPs that were previously characterized(regarding hydrodynamic size, zeta potential, and optical properties), and were used to label these cells,analyze cell viability via the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and BLI analysis, and quantify the internalization process and iron load in different concentrations of MNPs via magnetic resonance imaging(MRI),near-infrared fluorescence(NIRF), and inductively coupled plasma-mass spectrometry(ICP-MS). In in vivo analyses, the same labeled cells were implanted in a sham group and a stroke group at different times and under different MNP concentrations(after 4 h or 6 d of cell implantation) to evaluate the sensitivity of triple-modal *** hBM-MSC collection and isolation after immunophenotypic characterization were demonstrated to be adequate in hBM samples. After transduction of these cells with

关键词： Multimodal nanoparticles Human bone marrow mesenchymal stem cells Near-infrared fluorescence image Magnetic resonance image Bioluminescence Stroke

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