检索结果-南通市图书馆

McMYB4 improves temperature adaptation by regulating phenylpropanoid metabolism and hormone signaling in apple

Horticulture Research 2021年第1期8卷 2319-2332页

作者： Suxiao Hao Yanfen Lu Zhen Peng Enying Wang linke chao Silin Zhong Yuncong Yao Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of AgricultureBeijing 102206China Beijing Bei Nong Enterprise Management Co.Ltd Beijing 102206China Plant Science and Technology College Beijing University of AgricultureBeijing 102206China Beijing Key Laboratory for Agricultural Application and New Technique Beijing University of AgricultureBeijing 102206China College of Forestry Beijing Forestry UniversityBeijing 100083China College of Life Science The Chinese University of Hong KongHong KongChina

Temperature changes affect apple development and *** metabolism and hormone signaling play a crucial role in regulating apple growth and development in response to temperature ***,we found that McMYB4 is induced by treatment at 28℃ and 18℃,and McMYB4 overexpression results in flavonol and lignin accumulation in apple *** one-hybrid(Y1H)assays and electrophoretic mobility shift assays(EMSAs)further revealed that McMYB4 targets the promoters of the flavonol biosynthesis genes CHS and FLS and the lignin biosynthesis genes CAD and ***4 expression resulted in higher levels of flavonol and lignin biosynthesis in apple during growth at 28℃ and 18℃ than during growth at 23℃.At 28℃ and 18℃,McMYB4 also binds to the AUX/ARF and BRI/BIN promoters to activate gene expression,resulting in acceleration of the auxin and brassinolide signaling *** together,our results demonstrate that McMYB4 promotes flavonol biosynthesis and brassinolide signaling,which decreases ROS contents to improve plant resistance and promotes lignin biosynthesis and auxin signaling to regulate plant *** study suggests that McMYB4 participates in the abiotic resistance and growth of apple in response to temperature changes by regulating phenylpropanoid metabolism and hormone signaling.

关键词： phenyl acceleration metabolism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dysplasia of male organs induces apomixis in Malus crabapples

引用

Ornamental Plant Research 2021年第1期1卷 98-113页

作者： Yujing Hu Yanfen Lu linke chao Zhen Wang Yufen Bu Jie Zhang Wenhe Wang Yuncong Yao Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of AgricultureBeijing 102206China Plant Science and Technology College Beijing University of AgricultureBeijing 102206China Beijing Key Laboratory for Agricultural Application and New Technique Beijing 102206China Beijing Nursery Engineering Research Centre for Fruit Crops Beijing 102206China College of Landscape Architecture Beijing University of AgricultureBeijing 102206China

Apomixis has high value in fruit crop propagation and breeding applications,particularly for the transgenerational fixation of *** this study,we found that flowers of the triploid Malus cultivar'Royalty'that were bagged after emasculation(EB)and pollinated with pollen from the diploid cultivar'Flame'(EFB)produced high fruit set and increased seed numbers per *** offspring exhibited superior growth vigour compared to those of the diploid sister cultivar'Flame'.The fruit setting rate for'Royalty'EFB was 98.44%,which was much higher than the fruit setting rate for'Royalty'BA(Bagging without emasculation)at 19.7%.The fruit setting rate for'Royalty'EB was 47.46%compared to 19.7%for'Royalty'***,offspring derived from either'Royalty'EB or EFB exhibited superior growth vigour compared to those of the diploid'Flame'.These results suggested that'Royalty'exhibits facultative apomixis and has strong sexual reproductive ability under the stimulation of foreign ***,'Royalty'anthers did not dehisce after flowering and had malformed pollen grains and a low pollen germination ***,we proposed that dysplasia of the male organ could be an important driver factor of apomixis in'Royalty'.Our study provides new insight into the mechanism of apomixis in Malus crabapples.

关键词： crab cultivar organs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

不同pH溶液中四环素类抗生素与金属离子配合前后的光化学性质及毒性差异

引用

环境科学学报 2024年第2期44卷 136-144页

作者：崔芳溪张蓬曹胜凯郑智轩朱超葛林科陕西科技大学环境科学与工程学院西安710021 Lancaster Environment Centre Lancaster UniversityLancaster LA14YQUnited Kingdom

水环境特别是金属复合污染水体中,可酸碱解离抗生素-金属配合物是普遍存在的新污染物,但其配合形态的光化学性质和毒性却少有报道.本文考察了四环素(TC)、土霉素(OTC)两种四环素类抗生素(TCs)与金属离子Fe(Ⅲ)、Cu(Ⅱ)、Al(Ⅲ)在不同pH... 详细信息

水环境特别是金属复合污染水体中,可酸碱解离抗生素-金属配合物是普遍存在的新污染物,但其配合形态的光化学性质和毒性却少有报道.本文考察了四环素(TC)、土霉素(OTC)两种四环素类抗生素(TCs)与金属离子Fe(Ⅲ)、Cu(Ⅱ)、Al(Ⅲ)在不同pH条件下配合前后的典型光谱学性质变化,并通过发光细菌(Vibrio fischeri)评估了配合前后体系的毒性变化.结果表明,两种TCs均能在不同解离形态下与3种金属离子发生配合,配位位点为酮基基团上的碳氧双键.TCs在碱性条件下比中性和酸性条件下更易配合,并且对3种金属离子的配合能力大小依次为Fe(Ⅲ)>Cu(Ⅱ)>Al(Ⅲ).在pH=7.0时,TCs与金属离子的配合会导致紫外吸收峰减弱甚至消失,在多数情况下使得紫外吸收光谱出现不同程度红移.这种配合反应的发生也使得抗生素在短波长处的荧光强度增大,这是因为配位作用形成了氧桥型杂多核配合物,增加了平面的刚性结构.毒性实验表明,TC与Fe(Ⅲ)、Cu(Ⅱ)配合后对Vibrio fischeri的毒性增强,与Al(Ⅲ)配合后毒性减弱,而OTC与3种金属离子配合后毒性均表现为增强.以上结果揭示了不同pH溶液中TCs与金属离子配合前后的光化学性质及毒性具有差异,这对于准确评价金属复合污染的水环境中抗生素类新污染物的光化学行为和风险具有重要意义.

关键词：四环素类抗生素金属配合光化学性质光谱分析毒性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

水中与冰中多环芳烃衍生物对发光细菌(Vibrio fischeri)的光修饰毒性

引用

环境化学 2024年第8期43卷 2854-2863页

作者：郑智轩崔芳溪王子宇葛林科张蓬朱超马宏瑞陕西科技大学环境科学与工程学院西安710021

多环芳烃衍生物(SPAHs)是一类普遍存在、来源广泛、风险隐蔽的新污染物,可由多环芳烃(PAHs)通过微生物及化学作用转化生成,其毒性甚至比母体PAHs更强,研究其环境行为和毒性效应具有重要意义.本研究选取羟基多环芳烃(OH-PAHs)、硝基多环... 详细信息

多环芳烃衍生物(SPAHs)是一类普遍存在、来源广泛、风险隐蔽的新污染物,可由多环芳烃(PAHs)通过微生物及化学作用转化生成,其毒性甚至比母体PAHs更强,研究其环境行为和毒性效应具有重要意义.本研究选取羟基多环芳烃(OH-PAHs)、硝基多环芳烃(N-PAHs)和氯代多环芳烃(Cl-PAHs)共10种模型化合物,考察了在模拟日光(λ>290 nm)照射下,水中与冰中这些典型SPAHs对发光细菌Vibrio fischeri的光修饰毒性.毒性实验表明,对于OH-PAHs,1-羟基萘、4-氯-1-羟基萘在水中与冰中均表现出显著的光修饰毒性(P<0.05),而4-硝基-1-羟基萘仅在水相表现出较小的光修饰毒性;对于N-PAHs,1-硝基芘在水中与冰中均表现出光修饰毒性,2-硝基芴仅在冰中表现出光修饰毒性;Cl-PAHs中,仅1-氯芘在水中表现出光修饰毒性,而3种Cl-PAHs在冰中均表现出显著的光修饰毒性.通过HPLC-MS/MS分析,OH-PAHs光转化主要涉及的反应路径为取代基脱除、光氧化与苯环羟基化.以上结果阐明了SPAHs通过光化学转化可生成具有较高毒性的中间产物,对Vibrio fischeri表现为光修饰毒性,且水中与冰中的光修饰毒性具有差异,这有助于更准确评价寒冷地区这些典型新污染物的环境风险.

关键词：多环芳烃衍生物光修饰毒性发光细菌结冰环境.

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

衢枳壳HPLC指纹图谱的建立及特征成分分析

引用

中国现代应用药学 2018年第8期35卷 1217-1220页

作者：岳超马临科宋剑锋张文婷赵维良浙江省食品药品检验研究院杭州310052 衢州市食品药品检验研究院浙江衢州324002

目的建立衢枳壳的HPLC指纹图谱方法,并联合聚类分析技术,评价衢枳壳的质量。方法色谱柱采用Extend XDB C18(250 mm×4.6 mm,5μm),以乙腈-0.1%甲酸溶液为流动相进行梯度洗脱,检测波长为330 nm,柱温为30℃,流速为1.0 m L·min-1。结果通... 详细信息

目的建立衢枳壳的HPLC指纹图谱方法,并联合聚类分析技术,评价衢枳壳的质量。方法色谱柱采用Extend XDB C18(250 mm×4.6 mm,5μm),以乙腈-0.1%甲酸溶液为流动相进行梯度洗脱,检测波长为330 nm,柱温为30℃,流速为1.0 m L·min-1。结果通过11批衢枳壳样品建立了对照指纹图谱,得到19个共有峰,利用多级质谱信息,结合对照品和文献对9个共有峰进行指认,同时采用相似度分析和系统聚类分析方法对指纹图谱数据进行模式识别研究。衢枳壳相似度结果为0.995~1.000,并聚为一类,说明其质量较为一致,而其他混淆品差异性较大,另聚为一类。结论该方法简便、快速,专属性强,可以有效地评价衢枳壳的质量。

关键词：衢枳壳混淆品种指纹图谱聚类分析质量评价

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于网络药理学和体外细胞实验研究土茯苓抗骨质疏松的机制

引用

巴楚医学 2022年第3期5卷 69-74页

作者：高诗尧宁超章镇宇李育泉黄林科谭桢广西医科大学广西再生医学重点实验室广西南宁530022 广西医科大学第二附属医院骨科广西南宁530007

目的:基于网络药理学和体外细胞实验探索土茯苓治疗骨质疏松的可能机制。方法:运用中药系统药理学数据库与分析平台(TCMSP)筛选土茯苓活性成分及作用靶点,利用人类基因数据库(GeneCards数据库)筛选骨质疏松的靶点,两者取交集获取共同靶... 详细信息

目的:基于网络药理学和体外细胞实验探索土茯苓治疗骨质疏松的可能机制。方法:运用中药系统药理学数据库与分析平台(TCMSP)筛选土茯苓活性成分及作用靶点,利用人类基因数据库(GeneCards数据库)筛选骨质疏松的靶点,两者取交集获取共同靶点然后构建蛋白相互作用网络并进行可视化分析,同时进行GO功能注释富集分析(GO分析)及京都基因与基因组百科全书(KEGG)富集分析。采用MC3T3-E1成骨细胞系分化实验验证土茯苓对骨分化的作用。结果:预测到土茯苓129个有效靶点和骨质疏松相关靶点2287个,共同靶点68个。GO分析涉及细胞因子受体结合、趋化因子受体结合、核受体活性。KEGG富集分析显示,靶点可能通过HIF-1信号通路、AMPK信号通路、Wnt信号通路等发挥作用。细胞实验表明土茯苓可以促进MC3T3-E1成骨细胞碱性磷酸酶的表达。结论:土茯苓可能通过促进成骨细胞分化从而防治骨质疏松。

关键词：土茯苓网络药理学骨质疏松成骨细胞

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Tolerance and acceptance of hepatic venous pressure gradient measurement in cirrhosis(CHESS1904):An international multicenter study

引用

Portal Hypertension & Cirrhosis 2022年第1期1卷 7-14页

作者： Jun‐Hui Sun He Zhao Haijun Zhang Lei Li NecatiÖrmeci Zi‐Niu Yu Xun Li Shuangxi Li Xujun Yang Huaping Wei Xiaoliang Zhu Zhengcong Zhang Yajin Wang Zhongwei Zhao Jianting Mao Qiaohong Wu Xiaole Sun Huiling Xiang Kefeng Jia chao Yang Wei Wu Xiuqing Lin Haixin Yao Changzeng Zuo Jitao Wang Bo Zhang Chunqing Zhang Xiaoling Wu Guangchuan Wang Shengjuan Yao Ruihang Wang Li Zhou Hui Huan Qingli Tu Xue Pu Feng Zhang Qin Yin Linpeng Zhang Ying Guo Jian Wang Kohei Kotani Sawako Uchida‐Kobayashi Norifumi Kawada He Zhu Li Li Wei Wang Guo Zhang Lei Yu Xudong Cui Qingliang Zhu Hailong Zhang Xiaoli Hu Rafael OXimenes Adriano Gonçalves de Araújo Giulliano Gardenghi Yubao Zheng Zebin Wu Mingsheng Huang Xiaoyong Chen Jun Wu Feng Xie Yang Bo Shengjuan Hu linke Ma Xiao Li Xiaolong Qi Hepatobiliary and Pancreatic Interventional Treatment Center Division of Hepatobiliary and Pancreatic SurgeryThe First Affiliated HospitalZhejiang University School of MedicineZhejiang Clinical Research Center of Hepatobiliary and Pancreatic DiseasesZhejiang Provincial Research Center for Diagnosis and Treatment of Heapatobiliary DiseasesZhejiang University Cancer CenterHangzhouZhejiangChina Department of Interventional Therapy National Cancer Center/National Clinical Research Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Institute of Portal Hypertension Department of General SurgeryThe First Hospital of Lanzhou UniversityLanzhouGansuChina Department of Anesthesiology and Operating Theater The First Hospital of Lanzhou UniversityLanzhouGansuChina Interventional Radiology Department The First Hospital of Lanzhou UniversityLanzhouGansuChina Department of Gastroenterology Ankara University School of MedicineAnkaraTurkey Department of Internal Medicine Istanbul Health and Technology University Medical SchoolIstanbulTurkey Department of General Surgery Key Laboratory of Biotherapy and Regenerative Medicine of Gansu ProvinceThe First Hospital of Lanzhou UniversityLanzhouGansuChina Nursing Department The First Hospital of Lanzhou UniversityLanzhouGansuChina Department of General Surgery Donggang BranchFirst Hospital of Lanzhou UniversityLanzhouGansuChina The Diagnosis and Treatment Center of Endoscopy and Interventional Radiology Department of General SurgeryDonggang BranchFirst Hospital of Lanzhou UniversityLanzhouGansuChina Department of Interventional Radiology Lishui Central HospitalLishuiZhejiangChina Department of Hepatology and Gastroenterology of The Third Central Hospital of Tianjin TianjinChina Department of Radiology of The Third Central Hospital of Tianjin TianjinChina Department of Gastroenterology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiangChina Department of Hepatobiliar

Aim:To determine the tolerance and acceptance of hepatic venous pressure gradient(HVPG)measurements in patients with liver ***:This prospective international multicenter study included 271 patients with cirrhosis who were scheduled to undergo HVPG measurement between October 2019 and June *** related to the tolerance and acceptance of HVPG measurements were collected using descriptive ***:HVPG measurements were technically successful in all 271 patients,with 141(52.0%)undergoing HVPG measurement *** complication rate was 0.4%.Postoperative pain was significantly lower than preoperative expected pain(p<0.001)and intraoperative pain(p<0.001),and intraoperative pain was also significantly lower than preoperative expected pain(p=0.036).No,mild,moderate,severe,and intolerable discomfort scores were reported by 36.9%,44.6%,11.1%,6.3%,and 0.4%of these patients,respectively,during HVPG measurement and by 54.6%32.5%,11.4%,1.5%,and 0%,respectively,after HVPG *** these patients,39.5%had little understanding and 10%had no understanding of the value of HVPG measurement,with 35.1%and 4.1%regarding HVPG measurements as being of little or no help,*** patients reported that they would definitely(15.5%),probably(46.9%),or possibly(29.9%)choose to undergo additional HVPG measurements again,and 62.7%regarded the cost of the procedure as ***:HVPG measurement was safe and well‐tolerated in patients with cirrhosis,but patient education and communication are warranted to improve the acceptance of this procedure.

关键词： design hypertension multicenter study portal pressure prospective study questionnaire wedge

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：