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Core–Shell Microfiber Encapsulation Enables Glycerol‑Free Cryopreservation of RBCs with High Hematocrit

Nano-Micro Letters 2024年第1期16卷 35-54页

作者： Xianhui Qin Zhongrong Chen lingxiao shen Huilan Liu Xilin Ouyang Gang Zhao Department of Electronic Engineering and Information Science University of Science and Technology of ChinaHefei 230027People’s Republic of China School of Biomedical Engineering Anhui Medical UniversityHefei 230022People’s Republic of China Department of Blood Transfusion The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001People’s Republic of China The Fourth Medical Center Chinese PLA General HospitalBeijing 100089People’s Republic of China

Cryopreservation of red blood cells(RBCs)provides great potential benefits for providing transfusion timely in *** concentrations of glycerol(20%or 40%)are used for RBC cryopreservation in current clinical practice,which results in cytotoxicity and osmotic injuries that must be carefully ***,existing studies on the low-glycerol cryopreservation of RBCs still suffer from the bottleneck of low hematocrit levels,which require relatively large storage space and an extra concentration process before transfusion,making it inconvenient(time-consuming,and also may cause injury and sample lose)for clinical *** this end,we develop a novel method for the glycerol-free cryopreservation of human RBCs with a high final hematocrit by using trehalose as the sole cryoprotectant to dehydrate RBCs and using core–shell alginate hydrogel microfibers to enhance heat transfer during *** from previous studies,we achieve the cryopreservation of human RBCs at high hematocrit(>40%)with high recovery(up to 95%).Additionally,the washed RBCs post-cryopreserved are proved to maintain their morphology,mechanics,and functional *** may provide a nontoxic,high-efficiency,and glycerol-free approach for RBC cryopreservation,along with potential clinical transfusion benefits.

关键词： Cryopreservation Human red blood cells Core-shell microfibers Trehalose Hematocrit

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Ionic Liquid-Based Ultrasound-Assisted Emulsification Microextraction Coupled with HPLC for Simultaneous Determination of Glucocorticoids and Sex Hormones in Cosmetics

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Green and Sustainable Chemistry 2013年第2期3卷 26-31页

作者： Xiaoji Cao lingxiao shen Xuemin Ye Feifei Zhang Jiaoyu Chen Weimin Mo Research Center of Analysis and Measurement Zhejiang University of Technology Hangzhou China

An effective and environmentally friendly method based on ionic liquid-based ultrasound-assisted emulsification microextraction(IL-USAEME) and high performance liquid chromatography (HPLC) has been developed for the determination of nine hormones including three glucocorticoids, one androgen and five progestogens in cosmetics. Several factors that affect the extraction efficiency, such as the kinds and volume of ionic liquid, salt concentration, ultrasonic power and time, and centrifugation time were investigated and optimized. Under the optimum extraction condition, the recoveries of analytes ranged from 85.97% to 108.84% except prednisolone (62.30%). The intraday and interday precision was below 2.51% and 3.30%, respectively.

关键词： Ionic Liquid Ultrasound-Assisted Emulsification Microextraction Hormones Cosmetics High Performance Liquid Chromatography

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浅谈自制体育玩具在幼儿园户外活动中的运用

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当代家庭教育 2021年第4期 61-62页

作者：沈玲晓江苏省吴江经济技术开发区山湖花园幼儿园

幼儿园户外活动是幼儿园教育的重要内容之一,是促进幼儿生长发育、增强幼儿体质、放松幼儿情绪的良好途径。幼儿参与制作体育玩具,能激发幼儿参与户外活动的兴趣,在自主探索游戏中,也能更好地发挥幼儿的主体性地位,学会一物多玩,体验游... 详细信息

幼儿园户外活动是幼儿园教育的重要内容之一,是促进幼儿生长发育、增强幼儿体质、放松幼儿情绪的良好途径。幼儿参与制作体育玩具,能激发幼儿参与户外活动的兴趣,在自主探索游戏中,也能更好地发挥幼儿的主体性地位,学会一物多玩,体验游戏的乐趣,促进幼儿健康快乐地成长。

关键词：自制体育玩具户外活动运用

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浅谈自制体育玩具在幼儿园户外活动中的运用

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当代家庭教育 2021年第9期 52-53页

作者：沈玲晓江苏省吴江经济技术开发区山湖花园幼儿园

关键词：自制体育玩具户外活动运用

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标准物质在临床质谱技术中的应用

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中华检验医学杂志 2021年第5期44卷 437-441页

作者：沈凌晓马金飞杨青青刘鹏云刘华芬杭州凯莱谱精准医疗检测技术有限公司杭州310000

标准物质是实现检验结果准确可比的主要技术手段之一,而质谱技术作为一种临床检验新技术,在使用标准物质时同样需要区分标准物质的不同作用,合理选择并应用于测量方法确认、校准及质量控制等方面,从而保证检验结果的准确性和可比性。本... 详细信息

标准物质是实现检验结果准确可比的主要技术手段之一,而质谱技术作为一种临床检验新技术,在使用标准物质时同样需要区分标准物质的不同作用,合理选择并应用于测量方法确认、校准及质量控制等方面,从而保证检验结果的准确性和可比性。本文从标准物质在临床质谱中存在的问题出发,对于标准物质在测量方法确认、校准物及质量控制等方面的使用注意事项进行了介绍和总结。

关键词：标准物质临床质谱技术应用

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TAKAP: A Lightweight Three-Party Authenticated Key Agreement Protocol with User Anonymity

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Chinese Journal of Electronics 2021年第4期30卷 686-696页

作者： QIAO Hui DONG Xuewen shen Yulong LIU Haozhe YANG lingxiao School of Computer Science and Technology Xidian University Shaanxi Key Laboratory of Network and System Security Xidian University

The three-party authenticated key agreement protocol is a significant cryptographic mechanism for secure communication, which encourages two entities to authenticate each other and generate a shared session key with the assistance of a trusted party(remote server) via a public channel. Recently, Wang et al. put forward a three-party key agreement protocol with user anonymity and alleged that their protocol is able to resist all kinds of attacks and provide multifarious security features in Computer Engineering & Science, No.3, ***, we show that Wang et al.’s protocol is vulnerable to the password guessing attack and fails to satisfy user anonymity and perfect secrecy. To solve the aforementioned problems, a lightweight chaotic mapbased Three-party authenticated key agreement protocol(short for TAKAP) is proposed, which not only could provide privacy protection but also resist a wide variety of security attacks. Furthermore, it is formally proved under Burrows-Abadi-Needham(BAN) logic. Simultaneously,the performance analysis in this paper demonstrates that the proposed TAKAP protocol is more secure and efficient compared with other relevant protocols.

关键词： Three-party Authenticated key agreement Chaotic map Anonymity Security attacks

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液相色谱-串联质谱技术在维生素检测中的应用

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中华检验医学杂志 2019年第6期42卷 479-483页

作者：袁小芬马金飞杨青青刘鹏云沈凌晓张前军李义坤刘华芬杭州凯莱谱精准医疗检测技术有限公司杭州310000

维生素分为脂溶系列包括ADEK和水溶系列维生素B族及维生素C。目前只有对维生素D、B6、B9及B12开发了传统的免疫学的检测方法,但免疫法无法区分亚型比如维生素D2及D3以及在婴儿中含量较高的Epi同分异构体,因此免疫学方法检测经常会出现... 详细信息

维生素分为脂溶系列包括ADEK和水溶系列维生素B族及维生素C。目前只有对维生素D、B6、B9及B12开发了传统的免疫学的检测方法,但免疫法无法区分亚型比如维生素D2及D3以及在婴儿中含量较高的Epi同分异构体,因此免疫学方法检测经常会出现假阳性或假阴性。质谱技术以其快速、高分辨率、高灵敏度、高特异性等优点成为生物样本内小分子分析的金标准方法,广泛应用于临床研究及诊断,也为减少样品采集量及一次进样同时检测多种维生素提供了高效方法。

关键词：维生素类色谱法,液相串联质谱法

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Pollution levels and characteristics of phthalate esters in indoor air of offices

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Journal of Environmental Sciences 2015年第2期27卷 157-162页

作者： Min Song Chenchen Chi Min Guo Xueqing Wang lingxiao Cheng Xueyou shen College of Environmental & Resource Sciences Zhejiang University Chinese Research Academy of Environmental Sciences The Xinhua Hospital of Zhejiang Province Zhejiang Provincial Key Laboratory of Organic Pollution and Control

The pollution status and characteristics of PAEs（phthalate esters） were investigated in indoor air of offices, and PAEs of both gas-phase and particulate-phase were detected in all the samples. The concentration（sum of the gas phase and the particulate phase） was4748.24 ng/m3, ranging between 3070.09 and 6700.14 ng/m3. Diethyl phthalate, dibutyl phthalate, and di（2-ethylhexyl） phthalate were the most abundant compounds, together accounting for 70% of the ∑ 6PAEs. Dividing the particulate-phase PAEs into four size ranges（〈 2.5, 2.5–5, 5–10, 〉 10 μm）, the result indicated that PAEs in PM2.5were the most abundant,with the proportion of 72.64%. In addition, the PAE concentration in PM2.5correlated significantly with the total particulate-phase PAEs（R2= 0.85）. Thus, the amount of PAEs in PM2.5can be estimated from the total amount of particulate-phase PAEs using this proportion. In a comparison between the offices and a newly decorated study room, it was found that pollution characteristics were similar between these two places. Thus, it is implied that the PAE concentration decreased by 50% 2 yr after decorating.

关键词： Phthalate esters Pollution status Particle size distribution Indoor air Office

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Spatiotemporal Patterns of Road Network and Road Development Pri-ority in Three Parallel Rivers Region in Yunnan,China:An Evaluation Based on Modified Kernel Distance Estimate

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Chinese Geographical Science 2014年第1期24卷 39-49页

作者： YING lingxiao shen Zehao CHEN Jiding FANG Rui CHEN Xueping JIANG Rui Department of Ecology College of Urban&Environmental Sciencesthe MOE Laboratory of Earth Surface ProcessesPeking University Center of Transportation Environmental Protection and Security Chinese Academy of Transportation Science Yunnan Highway Planning Reconnaissance Design Institute

Road network is a critical component of public infrastructure,and the supporting system of social and economic *** on a modified kernel density estimate(KDE)algorithm,this study evaluated the road service capacity provided by a road network composed of multi-level roads(***,provincial,county and rural roads),by taking account of the differences of effect extent and intensity for roads of different *** at town scale,the population burden and the annual rural economic income of unit road service capacity were used as the surrogates of social and economic demands for road *** method was applied to the road network of the Three Parallel River Region,the northwestern Yunnan Province,China to evaluate the development of road network in this *** results,the total road length of this region in 2005 was 3.70×104km,and the length ratio between national,provincial,county and rural roads was 1∶2∶8∶*** 1989 to 2005,the regional road service capacity increased by 13.1%,of which the contributions from the national,provincial,county and rural roads were 11.1%,19.4%,22.6%,and 67.8%,respectively,revealing the effect of′All Village Accessible′policy of road development in the mountainous regions in the last *** spatial patterns of population burden and economic requirement of unit road service suggested that the areas farther away from the national and provincial roads have higher road development priority(RDP).Based on the modified KDE model and the framework of RDP evaluation,this study provided a useful approach for developing an optimal plan of road development at regional scale.

关键词： road network kernel density estimate(KDE) road service road development priority(RDP) Three Parallel Rivers Region China

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Flexible liquid metal coil prepared for electromagnetic energy harvesting and wireless charging

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Frontiers in Energy 2019年第3期13卷 474-482页

作者： shen GUO Peng WANG Jichuan ZHANG Wenpeng LUAN Zishuo XIA lingxiao CAO Zhizhu HE China Electric Power Research Institute Beijing 100192China Vehicle Engineering College of EngineeringChina Agricultural UniversityBeijing 100083China Tianjin Microfluidic Technology Co. Ltd.Tianjin 300457China

This paper reported a study on a flexible liquid metal coil (LMC) for electromagnetic collection from the transmission line for self-powered sensor and electromagnetic generation for wireless charging of cellular telephone. The room temperature liquid metal of Galinstan was perfused to elastic silicone tube, which is then terminated with galliumplated copper wire. The asprepared liquid metal wire can sustain stretching, twisting, and bending with large deformation, and has a good electrical contact stability with the external circuit. The LMC based magnetic energy harvester was then designed and demonstrated to collect the magnetic field energy induced by a wire carrying alternating current. The power of 260 mW was obtained for the wire carrying current of 10 A. The flexible toroidal inductor was fabricated and tested for magnetic energy harvesting. The flexible spiralshaped LMC was also designed and demonstrated to power cellular telephone through wireless charging. The present study opens the way for further applications of elastic LMC in electromagnetic energy harvesting and charging.

关键词： liquid metal Galinstan flexible coil electromagnetic energy harvesting wireless charging

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