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Evaluation of the passivation effect and the first-principles calculation on surface termination of germanium detector

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Nuclear Science and Techniques 2021年第9期32卷 40-51页

作者： Sha-Sha lv Yuan-Yuan Liu Wei-You Tang Li He Xiu-Xia Li Jian-Ping Cheng Key Laboratory of Beam Technology of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China Department of Engineering Physics Tsinghua UniversityBeijing 100084China Joint Research Center Nuctech Company LimitedBeijing 100084China

The point-contact high-purity germanium detector(HPGe)has the advantages of low background,low energy threshold,and high energy resolution and can be applied in the detection of rare-event ***,the performance of HPGe must be further improved to achieve superior energy resolution,low noise,and long-term *** this study,we combine computational simulations and experimental comparisons to deeply understand the passivation mechanism of *** surface passivation effect is calculated and inferred from the band structure and density of interface states,and further con-firmed by the minority carrier *** first-principles method based on the density functional theory was adopted to systematically study the lattice structure,band structure,and density of state(DOS)of four different systems:Ge–H,Ge–Ge-NH 2,Ge-OH,and Ge-SiO_(x).The electronic char-acteristics of the Ge(100)unit cell with different passi-vation groups and Si/O atomic ratios were *** shows that H,N,and O atoms can effectively reduce the surface DOS of the Ge *** passivation effect of the SiO_(x) group varied with increasing O atoms and Si/O atomic ***,SiO and SiO_(2) passivation films were fabricated by electron beam evaporation on a Ge substrate,and the valence state of Si and resistivity was measured to characterize the *** minority carrier lifetime of Ge-SiO_(2) is 21.3 ls,which is approximately quadruple that of *** passivation effect and mechanism are discussed in terms of hopping conduction and surface defect *** study builds a relationship between the passivation effect and different termination groups,and provides technical support for the potential passivation layer,which can be applied in Ge detectors with ultralow energy thresholds and especially in HPGe for rare-event physics detection experiments in future.

关键词： Germanium detector Passivation Surface termination

2020—2021年保山市有色米水稻品种区域试验

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云南农业科技 2024年第1期 22-24页

作者：王勤张朝钟万卫东吕宏斌张晓兰陶加进董诗铝保山市隆阳区农业技术推广所云南隆阳678000 云南省农业科学院粮食作物研究所云南昆明650205

按《云南省农业科学院粮食作物研究所特殊用途水稻品种试验实施方案》,2020—2021年连续2年进行有色米水稻品种多点试验示范。结果表明,3个参试品种中,综合性状较好的是红两优8012,推荐进入云南省水稻品种审定。

关键词：有色米水稻品种区域试验生产试验

A novel cold-inducible promoter,PThCAP from Tamarix hispida,confers cold tolerance in transgenic Arabidopsis thaliana

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Journal of Forestry Research 2018年第2期29卷 330-336页

作者： Xiaohong Guo Yandong lv Hongyu Li Nan Fu Guiping Zheng Lihua Liu Yuhua Li State Key Laboratory of Tree Genetics and Breeding Northeast Forestry University Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province Agronomy College Heilongjiang Bayi Agricultural University College of Life Science and Technology Heilongjiang Bayi Agricultural University College of Life Sciences Daqing Bio-tech Research InstituteNortheast Forestry University

Our previous studies have revealed that the Th CAP gene plays a vital role in transgenic Populus(*** 9 ***) in response to cold ***,the regulatory mechanism of Th CAP gene expression has been *** this study,the 50 flanking region of the Th CAP promoter(PTh CAP) was cloned using a genomewalking *** analyzing cis-acting regulatory elements of PTh CAP,a DRE motif and MYC and MYB elements were found to be located in the *** identify the regulatory elements that control the expression of the Th CAP gene promoter,a series of deletion derivatives ofPTh CAP,P1–P5,from the translation start code(-1538,-1190,-900,-718 and-375 bp),were fused to the GUS reporter gene,and then each deletion was stably introduced into Arabidopsis thaliana *** analysis of the promoter suggested that only the P2 fragment had strong GUS expression in leaves and roots of *** exposed to low temperature *** results suggest that this290-bp region(-1190 to-900 bp),as an important part in PTh CAP,was associated with cold tolerance of *** results provide evidence for the regulatory mechanism of Th CAP gene involved in the response to cold stress,and that the gene is promising candidate gene for genetic improvement of crops.

关键词： Arabidopsis thaliana Cis-acting elements GUS expression PThCAP ThCAP gene

Advances in osseointegration of biomimetic mineralized collagen and inorganic metal elements of natural bone for bone repair

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Regenerative Biomaterials 2023年第1期10卷 808-829页

作者： Wenbo Zhu Chao Li Mengxuan Yao Xiumei Wang Juan Wang Wei Zhang Wei Chen Hongzhi lv Department of Orthopaedic Surgery The Third Hospital of Hebei Medical UniversityShijiazhuang 050051P.R.China Key Laboratory of Biomechanics of Hebei Province Orthopaedic Research Institution of Hebei ProvinceShijiazhuang 050051P.R.China NHC Key Laboratory of Intelligent Orthopaedic Equipment The Third Hospital of Hebei Medical UniversityShijiazhuang 050051P.R.China State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing 100084P.R.China Department of Pathology Hebei Key Laboratory of NephrologyCenter of Metabolic Diseases and Cancer ResearchHebei Medical UniversityShijiazhuang 050017P.R.China

At this stage,bone defects caused by trauma,infection,tumor,or congenital diseases are generally filled with autologous bone or allogeneic bone transplantation,but this treatment method has limited sources,potential disease transmission and other *** bone-graft materials remain continuously explored,and bone defect reconstruction remains a significant *** collagen prepared by bionic mineralization combining organic polymer collagen with inorganic mineral calcium phosphate can effectively imitate the composition and hierarchical structure of natural bone and has good application value in bone repair ***,strontium,zinc and other inorganic components not only can activate relevant signaling pathways to induce differentiation of osteogenic precursor cells but also stimulate other core biological processes of bone tissue growth and play an important role in natural bone growth,and bone repair and *** study reviewed the advances in hydroxyapatite/collagen composite scaffolds and osseointegration with natural bone inorganic components,such as magnesium,strontium and zinc.

关键词： mineralized collagen bone defect reconstruction inorganic metal elements clinical applications

Varying water column stability controls the denitrification process in a subtropical reservoir,Southwest China

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Journal of Environmental Sciences 2022年第1期34卷 208-219页

作者： Gaoyang Cui Xiao-Dong Li Siqi Li Shiyuan Ding Qinkai Li Mengdi Yang Hong lv Yiyao Wang Key Laboratory of Geospatial Technology for the Middle and Lower Yellow River Regions Ministry of EducationCollege of Environment and PlanningHenan UniversityKaifeng 475004China Institute of Surface-Earth System Science School of Earth System ScienceTianjin UniversityTianjin 300072China Tianjin Key Laboratory of Earth’s Critical Zone Science and Sustainable Development in Bohai Rim Tianjin UniversityTianjin 300072China

Reservoirs are regarded as hotspots of nitrogen transformation and potential sources of nitrous oxide(N_(2)O).However,it remains unclear how the hydrological conditions due to dam construction control the processes of nitrogen transformation in reservoir *** address this issue,we examined the spatial-temporal characteristics of nitrate concentrations,δ^(15)N-NO_(3)^(-),δ^(18)O-NO_(3)^(-),δ^(18)O-H_(2)O,relative water column stability(RWCS),and related environmental factors in a subtropical eutrophic reservoir(Hongfeng Reservoir,HFR),Southwest *** found that denitrification was the most important nitrogen transformation process in the HFR and that higher denitrification intensity was associated with increased RWCS in summer,which suggested hydrological control of the denitrification *** contrast,low RWCS conditions favored the nitrification process in the HFR in ***,dissolved oxygen(DO;pling/decoupling of nitrification-denitrification at the local and global *** study would advances our knowledge of the impacts of RWCS and thermal stratification on nitrogen transformation processes in reservoirs.

关键词： Thermal stratification RWCS Denitrification Nitrogen transformation Reservoir

Coordination-responsive drug release inside gold nanorod @ metal-organic framework core-shell nanostructures for near-infrared-induced synergistic chemo-photothermal therapy

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Nano Research 2018年第6期11卷 3294-3305页

作者： Yantao Li Jun Jin Dawei Wang Jiawei lv Ke Hou Yaling Liu Chunying Chen Zhiyong Tang CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 China

Multifunctional core-shell nanostructures formed by integration of distinct components have received wide attention as promising biological platforms in recent years. In this work, crystalline zeolitic imidazolate framework-8 （ZIF-8）, a typical metal-organic framework （MOF）, is coated onto single gold nanorod （AuNR） core for successful realization of synergistic photothermal and chemotherapy triggered by near-infrared （NIR） light. Impressivel）~ high doxorubicin hydrochloride （DOX） loading capacity followed by pH and NIR light dual stimuli-responsive DOX release can be easily implemented through formation and breakage of coordination bonds in the system. Moreover, under NIR laser irradiation at 808 nm, these novel AuNR@MOF core-shell nanostructures exhibit effective synergistic chemo-photothermal therapy both in vitro and in vivo, confirmed by cell treatment and tumor ablation via intravenous injection.

关键词： core-shell nanostructures coordination bonds dual stimuli response synergistic cancer therapy

不同乳腺癌细胞株胞外囊泡特性和差异miRNA自噬通路靶基因分析

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实用癌症杂志 2024年第7期39卷 1054-1060页

作者：吕爽贾永峰包玉龙施琳云芬刘霞王利内蒙古自治区人民医院 010017 内蒙古医科大学基础医学院 010017

目的研究不同乳腺癌细胞株EVs特性和所含miRNA的数量和功能差异。方法采用无血清培养基对正常乳腺上皮细胞HMEC和乳腺癌细胞株MDA-MB-231、MCF-7进行培养,通过纳米粒径分析对胞外囊泡颗粒大小和含量分布,利用投射电镜观察胞外囊泡形态... 详细信息

目的研究不同乳腺癌细胞株EVs特性和所含miRNA的数量和功能差异。方法采用无血清培养基对正常乳腺上皮细胞HMEC和乳腺癌细胞株MDA-MB-231、MCF-7进行培养,通过纳米粒径分析对胞外囊泡颗粒大小和含量分布,利用投射电镜观察胞外囊泡形态,采用Western bolt检测胞外囊泡膜蛋白标记物CD63。通过转录组测序对胞外囊泡中miRNA进行差异分析,利用GO和KEGG分析差异miRNA靶基因。结果通过多峰粒径分析显示,HMEC与MDA-MB-231、MCF-7胞外囊泡粒径分布、含量有所不同。三株细胞均可检测到具有典型特征胞外囊泡和标记物CD63的表达。HMEC、MCF-7、MDA-MB-231胞外囊泡中共有已知miRNA分别为613、333、698个。以HMEC为对照,进行差异比较分析发现,MCF-7的胞外囊泡中显著上调和下调的miRNA分别为108个和86个,MDA-MB-231的胞外囊泡中显著上调和下调的miRNA分别为135个和246个。GO和KEGG富集分析发现差异miRNA的靶基因涉及多种生物学功能,尤其细胞自噬。结论不同乳腺癌细胞株胞外囊泡从直径、数量分布、所含miRNA都与正常乳腺上皮细胞存在差异,不同乳腺癌细胞株之间也存在差异。乳腺癌细胞株胞外囊泡的差异miRNA可能对其自身或作用的靶细胞的自噬水平产生不同影响,其机制有待进一步研究揭示。

关键词：乳腺癌胞外囊泡纳米粒径分析 microRNA 自噬

稳定表达跨膜丝氨酸蛋白酶2基因的MDCK细胞系的建立

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中国预防兽医学报 2014年第9期36卷 704-707页

作者：吕让王丹宋彩玲齐宾宾刘明中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室黑龙江哈尔滨150001 黑龙江省医院黑龙江哈尔滨150001 东北农业大学动物医学学院黑龙江哈尔滨150030

为建立稳定表达跨膜丝氨酸蛋白酶2(TMPRSS2)基因的MDCK细胞系,本研究采用RT-PCR扩增TMPRSS2基因,克隆于逆转录病毒载体pLXSN中,构建重组质粒pLXSN-TMPRSS2。采用脂质体转染的方式将重组质粒pLXSN-TMPRSS2、pGP和pE-ampho共转染293T细胞... 详细信息

为建立稳定表达跨膜丝氨酸蛋白酶2(TMPRSS2)基因的MDCK细胞系,本研究采用RT-PCR扩增TMPRSS2基因,克隆于逆转录病毒载体pLXSN中,构建重组质粒pLXSN-TMPRSS2。采用脂质体转染的方式将重组质粒pLXSN-TMPRSS2、pGP和pE-ampho共转染293T细胞,获得逆转录病毒样颗粒。将逆转录病毒样颗粒感染MDCK细胞,经G418加压筛选和有限稀释法克隆及RT-PCR和间接免疫荧光(IFA)等试验的鉴定,建立了稳定表达TMPRSS2基因的MDCK细胞系。此外,红细胞凝集试验和IFA结果还显示,流感病毒可以在无外源胰酶存在的条件下在该细胞系中增殖,从而为流感细胞培养型病毒株的大规模生产奠定了基础。

关键词：跨膜丝氨酸蛋白酶2 逆转录病毒 MDCK细胞系 TMPRSS2

针刺治疗卒中后抑郁的神经–内分泌–免疫网络机制

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中国针灸 2024年第9期44卷 1100-1106页

作者：韩名媛高睿泽杨成妍吕晓琳孙忠人范颖王羽心尹洪娜黑龙江中医药大学研究生院哈尔滨150040 黑龙江中医药大学附属第二医院哈尔滨150000 黑龙江中医药大学附属第一医院

神经、内分泌和免疫系统之间借助神经递质、内分泌激素和细胞因子等联结成神经-内分泌-免疫(NEI)网络,多层次、多系统地将效果信息输出到靶器官。针刺效应可通过NEI网络级联放大,引起穴位局部微环境应答以治疗卒中后抑郁(PSD)。本文分... 详细信息

神经、内分泌和免疫系统之间借助神经递质、内分泌激素和细胞因子等联结成神经-内分泌-免疫(NEI)网络,多层次、多系统地将效果信息输出到靶器官。针刺效应可通过NEI网络级联放大,引起穴位局部微环境应答以治疗卒中后抑郁(PSD)。本文分别从神经、内分泌、免疫3个方面系统阐释针刺治疗PSD的机制,并结合NEI网络从整体观揭示针刺治疗PSD的潜在机制与生物学途径,以期为针刺治疗PSD的基础研究提供可靠理论。

关键词：卒中后抑郁针刺神经-内分泌-免疫机制探讨综述