检索结果-南通市图书馆

A CRISPR/RfxC as13d-mediated strategy for efficient RNA knockdown in mouse embryonic development

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Life Sciences 2024年

作者： Lin Zhang Shi-Meng Cao Hao Wu Meng Yan Jinsong Li ling-ling chen State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular Andrology Shanghai Institute of Biochemistry and Cell Biology Center for Excellence in Molecular Cell Science Chinese Academy of Sciences University of Chinese Academy of Sciences Key Laboratory of RNA Innovation Science and Engineering CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology University of Chinese Academy of Sciences Chinese Academy of Sciences Key Laboratory of Systems Health Science of Zhejiang Province School of Life Science Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences School of Life Science and Technology ShanghaiTech University New Cornerstone Science Laboratory Shenzhen

The growing variety of RNA classes, such as mRNAs, lncRNAs, and circRNAs, plays pivotal roles in both developmental processes and various pathophysiological conditions. Nonetheless, our comprehension of RNA functions in live organisms remains limited due to the absence of durable and effective strategies for directly influencing RNA levels. In this study, we combined the CRISPR-RfxCas13d system with spermlike stem cell-mediated semi-cloning techniques, which enabled the suppressed expression of different RNA species. This approach was employed to interfere with the expression of three types of RNA molecules: Sfmbt2 mRNA, Fendrr lncRNA, and circMan1a2(2,3,4,5,6). The results confirmed the critical roles of these RNAs in embryonic development, as their loss led to observable phenotypes, including embryonic lethality, delayed embryonic development, and embryo resorption. In summary, our methodology offers a potent toolkit for silencing specific RNA targets in living organisms without introducing genetic alterations.

关键词：

Factors influencing willingness to participate in ophthalmic clinical trials and strategies for effective recruitment

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2024年第1期17卷 157-163页

作者： Jia-En Liang Meng-Yin Liang En-En Zhang Yu-Ying Peng ling-ling chen Jia-Yu Deng Ting Lin Jun Fu Jia-Ni Zhang Shao-Li Li Fei Li Hui-Ming Xaio Wen-Min Huang Yu-Hong Liu State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangdong Provincial Clinical Research Center for Ocular DiseasesGuangzhou 510060Guangdong ProvinceChina

AIM:To explore the factors influencing individuals’willingness to participate in ophthalmic clinical ***:A questionnaire survey was conducted from January to April 2021 among patients and their family members at Zhongshan Ophthalmic Center,Sun Yat-sen University,in Guangzhou,*** survey gathered data on respondents’willingness,demographic and socioeconomic profiles,as well as their reasons and concerns regarding engagement in clinical ***:Of the 1078 residents surveyed(mean age 31.2±13.1y;65.8%females)in Guangzhou,749(69.5%)expressed a willingness to participate in future ophthalmic clinical *** characteristics associated with greater willingness included a younger age,lower annual income,higher education,prior participation experience,previous ophthalmic treatment,and a better understanding of clinical *** the exception of age,these characteristics were significantly linked to a higher *** primary barrier to participation,expressed by 64.8%of those willing and 54.4%of those unwilling,was“Uncertain efficacy”.In terms of motivations,the willing group ranked“Better therapeutic benefits”(35.0%),“Professional monitoring”(34.3%),and“Trust in healthcare professionals”(33.1%)as their top three reasons,whereas the unwilling participants indicated“Full comprehension of the protocol”(46.2%)as the key ***:This study reveals a substantial willingness to participate in ophthalmic clinical trials and demonstrates the predictive role of demographic and socioeconomic *** in motivators and concerns between willing and unwilling participants highlight the significance of tailored recruitment ***,the need for and trust in healthcare professionals stand out as powerful motivations,underscoring the importance of enhancing physician-patient relationships,adopting patient-centered communication approaches,and addressing individualized needs to improve accrual rates.

关键词： participation willingness recruitment strategy ophthalmic clinical trial

Char Plant:A De Novo Open Chromatin Region Prediction Tool for Plant Genomes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2021年第5期19卷 860-871页

作者： Yin Shen ling-ling chen Junxiang Gao Hubei Key Laboratory of Agricultural Bioinformatics College of InformaticsHuazhong Agricultural UniversityWuhan 430070China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China

Chromatin accessibility is a highly informative structural feature for understanding gene transcription regulation,because it indicates the degree to which nuclear macromolecules such as proteins and RNAs can access chromosomal *** have shown that chromatin accessibility is highly dynamic during stress response,stimulus response,and developmental ***,physical access to chromosomal DNA in eukaryotes is highly ***,current technologies such as DNase-seq,ATAC-seq,and FAIRE-seq reveal only a portion of the open chromatin regions(OCRs)present in a given ***,the genome-wide distribution of OCRs remains *** this study,we developed a bioinformatics tool called Char Plant for the de novo prediction of OCRs in plant *** develop this tool,we constructed a three-layer convolutional neural network(CNN)and subsequently trained the CNN using DNase-seq and ATACseq datasets of four plant *** model simultaneously learns the sequence motifs and regulatory logics,which are jointly used to determine DNA *** of these steps are integrated into Char Plant,which can be run using a simple command *** results of data analysis using Char Plant in this study demonstrate its prediction power and computational *** our knowledge,Char Plant is the first de novo prediction tool that can identify potential OCRs in the whole *** source code of Char Plant and supporting files are freely available from https://***/Yin-Shen/Char Plant.

关键词： Open chromatin region Chromatin accessibility Convolutional neural network De novo prediction Plant genome

Molecular engineering of an electron-transport triarylphosphine oxide-triazine conjugate toward high-performance phosphorescent organic light-emitting diodes with remarkable stability

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2020年第7期63卷 904-910页

作者： ling-ling chen ling Peng Lin-Ye Wang Xu-Hui Zhu Jian-Hua Zou Junbiao Peng State Key Laboratory of Luminescent Materials and Devices and Institute of Polymer Optoelectronic Materials and DevicesSouth China University of Technology(SCUT)Guangzhou 510640China Guangzhou New Vision Opto-Electronic Technology Co. LtdGuangzhou 510730China

Organic electron-transport materials are an essential component to boost performances and stability of organic light-emitting diodes. We present a robust organic electron-transport compound 3-(6-(3-(4,6-bis(4-biphenylyl)-1,3,5-triazin-2-yl)phenyl)pyridin-2-yl)phenyldiphenylphosphine oxide by facilely coupling the triphenylphosphine oxide moiety to the 2-phenyl-4,6-bis(4-biphenylyl)-1,3,5-triazine unit via a 2,6-pyridinylene linker. It is well soluble in weakly polar solvents and possesses a high Tg of 123 ℃ with an exceptional Td≈470 ℃ at 1% weight loss and deep HOMO/LUMO levels of ca.-6.45/-3.06 eV. The phosphorescent spectrum measured in solid state at 77 K reveals a notable triplet energy of 2.88 eV. n-Doping with 8-hydroxyquinolatolithium(Liq) produces the electron mobility value of 4.66×10-5–3.21×10-4 cm2 V-1 s-1@(2–5)×105 V ***, the contrasting solubility of the bromo reaction intermediate and the new compound in alcoholic solvents facilitates separation. The characterizations of bottom-and top-emission green phosphorescent OLEDs involving this single Liq-doped electron-transport layer reveal long stability. In particular, the latter provides outstanding performances with 77.4 cd A–1(corresponding to an EQE of 18.7%) and 86.8 lm W–1@ca. 1000 cd m-2, based on the green emitter bis(2-phenylpyridine)(2-(4-methyl-3-phenylphenyl)pyridine)iridium(Ⅲ). Moreover, driven by a constant current for ca. 640 h, the initial luminance of 1000 cd m–2 appears almost no decay.

关键词： electron-transport materials halogen impurities heterocycles lifetime phosphine oxides

Structural basis of SARS-CoV-2 3CL^pro and anti-COVID-19 drug discovery from medicinal plants

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2020年第4期10卷 313-319页

作者： Muhammad Tahir ul Qamar Safar MAlqahtani Mubarak AAlamri ling-ling chen College of Life Science and Technology Guangxi UniversityNanning530004PR China Hubei Key Laboratory of Agricultural Bioinformatics College of InformaticsHuazhong Agricultural UniversityWuhan430070PR China Department of Pharmaceutical Chemistry College of PharmacyPrince Sattam Bin Abdulaziz University11323AlkarjSaudi Arabia

The recent pandemic of coronavirus disease 2019(COVID-19)caused by SARS-CoV-2 has raised global health *** viral 3-chymotrypsin-like cysteine protease(3CL^pro)enzyme controls coronavirus replication and is essential for its life cycle.3CL^pro is a proven drug discovery target in the case of severe acute respiratory syndrome coronavirus(SARS-CoV)and Middle East respiratory syndrome coronavirus(MERS-CoV).Recent studies revealed that the genome sequence of SARS-CoV-2 is very similar to that of ***,herein,we analysed the 3CL^pro sequence,constructed its 3D homology model,and screened it against a medicinal plant library containing 32,297 potential anti-viral phytochemicals/traditional Chinese medicinal *** analyses revealed that the top nine hits might serve as potential anti-SARS-CoV-2 lead molecules for further optimisation and drug development process to combat COVID-19.

关键词： Coronavirus SARS-CoV-2 COVID-19 Natural products Protein homology modelling Molecular docking Molecular dynamics simulation

Dysregulation of circular RNAs in inflammation and cancers

在线全文

学校读者我要写书评

暂无评论

Fundamental Research 2023年第5期3卷 683-691页

作者： Jiayu Liu Fangqing Zhao ling-ling chen Shicheng Su Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Medical Research CenterSun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China Breast Tumor Center Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 200092China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesChinese Academy of SciencesHangzhou 310003China Department of Immunology Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510080China

Emerging lines of evidence have shown that the production of the covalently closed single-stranded circular RNAs is not splicing errors,but rather a regulated process with distinct biogenesis and *** RNAs are expressed in a cell type-and tissue-specific manner and often localize to specific subcellular regions or organelles for *** dysregulation of circular RNAs from birth to death is linked to the pathogenesis and progression of diverse *** review outlines how aberrant circular RNA biogenesis,subcellular location,and degradation are linked to disease progression,focusing on metaflammation and *** also discuss potential therapeutic strategies and obstacles in targeting such disease-related circular RNAs.

关键词： Circular RNA Metaflammation Biogenesis Degradation Subcellular localization

Phosphine oxide derivatives as a robust component for optoelectronics

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2020年第24期65卷 2033-2035,M0004页

作者： ling-ling chen Wan-Yi Tan Xu-Hui Zhu State Key Laboratory of Luminescent Materials and Devices Institute of Polymer Optoelectronic Materials and DevicesSouth China University of TechnologyGuangzhou 510640China

The P@O bond is strongly polar and electron-withdrawing and a potent coordination agent toward hard *** in 1998,Huang and co-workers[1]introduced triphenylphosphine oxide as a coligand into a luminescent terbium(Tb)complex for organic lightemitting diodes(OLEDs).Organic phosphine oxide derivatives endowed with luminescence and electron-transport properties have been extensively studied in optoelectronics ever *** recently,we have shown that an elegantly engineered electrontransport phosphine oxide is able to afford ultra-stable OLEDs[2].

关键词： OLEDs diodes endowed

What ecological factors favor the shift from distyly to homostyly? A study from the perspective of reproductive assurance

在线全文

学校读者我要写书评

暂无评论

Journal of Plant Ecology 2018年第4期11卷 645-655页

作者： Xian-Feng Jiang Xing-Fu Zhu ling-ling chen Qing-Jun Li Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMengla 666303China University of Chinese Academy of Sciences Beijing 10049China Laboratory of Ecology and Evolutionary Biology State Key Laboratory in Conservation and Utilization of Bioresources in YunnanYunnan UniversityKunmingYunnan 650091China

Aims Distyly is one of the most widespread floral polymorphisms pro-moting *** transition from obligate cross-fertilized distyly to predominantly self-fertilized homostyly is frequently documented in various ***,empirical stud-ies concerning the ecological factors connected with this transition are still *** chungensis,suggested to be evolving from distyly to homostyly,provides an ideal model for the study of the ecological factors concerned with this *** study ***-gensis to understand if autonomous self-fertilization would provide reproductive assurance for the self-fertilized homo-styled morph in the *** the incompatibility features of *** were tested with hand-pollination *** compared the capacity of autonomous self-fertilization between the distylous and homo-styled morph of *** in the field by excluding the pollinators with *** addition,the degrees of herkogamy of some *** plants were between the short-styled and homo-styled *** plants were studied to understand whether they were able to obtain greater reproductive assurance when the herkogamy in the flowers was *** Findings All three morphs of *** were highly self-and intra-morph *** degree of herkogamy positively correlated with the capacity for autonomous self-fertilization.A negative correlation between the degree of herkogamy and the magnitude of pollen limitation was found,but no significant correlation was found between the degree of herkogamy and the contribution of cross-fertilization to overall *** study suggests that reducing the degree of herkogamy can significantly increase the reproductive assurance for a self-compatible *** results provided evidence that the homo-styled morph of *** had the highest capacity for autonomous self-fertilization and the highest seed production in the field,because autonomous self-f

关键词： autonomous self-fertilization dimorphic incompatibility distyly homostyly Primula chungensis reciprocal herkogamy reproductive assurance

Appending triphenyltriazine to 1,10-phenanthroline: a robust electron-transport material for stable organic light-emitting diodes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2018年第7期63卷 446-451页

作者： Guang Jin Jun-Zhe Liu Jian-Hua Zou Xiao-Lan Huang Meng-Jiao He ling Peng ling-ling chen Xu-Hui Zhu Junbiao Peng Yong Cao State Key Laboratory of Luminescent Materials and Devices and Institute of Polymer Optoelectronic Materials and Devices South China University of Technology (SCUT)

There has been an increasing demand for high-performance and cost-effective organic electron-transport materials for organic light-emitting diodes （OLEDs）. In this contribution, we present a simple compound 3-（3-（4,6-diphenyl-l,3,5-triazin-2-yl）phenyl）-1,10-phenanthroline through the facile Pd-catalyzed coupling of a triphenyltriazine boronic ester with 3-hromo-1,10-phenanthroline. It shows a high Tg of 112℃. The ultraviolet photoelectron spectroscopy measurements reveal a deep HOMO level of -6.5 eV. The LUMO level is derived as -3.0 eV, based on the optical bandgap. The low-temperature solid-state phosphorescent spectrum gives a triplet energy of -2.36eV. n-Doping with 8-hydroxyquinolatolithium （Liq, 1：1） leads to considerably improved electron mobility of 5.2 × 10 -6 -5.8 × 10 -5 cm2 v-1 S-1 at E=（2-5） × 10 5Vcm -1, in contrast with the triarylphosphine oxide- phenantroline molecular conjugate we reported previously. It has been shown that through optimizing the device structure and hence suppressing polaron-exciton annihilation, introducing this single Liq-doped electron-transport layer could offer high-efficiency and stable phosphorescent OLEDs.

关键词： Electron transport materials Organic light-emitting diodes Phenanthroline Device stability Triazine