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Nanofluidics for single-cell analysis

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Chinese Chemical Letters 2022年第4期33卷 1752-1756页

作者： Zengnan Wu ling lin Department of Bioengineering Beijing Technology and Business UniversityBeijing 100048China State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBeijing 100029China

Living single-cell analysis is vital for cell biology, disease pathology, drug discovery and medical treatment. It is of great significance to reveal the law of creature and to explore the mechanism of serious disease. The conventional single cell analysis focuses on a large number of cells or cell lysis, in order to obtain the average information about cells. Therefore, it fails to analyze the real-time and continuous data of differences between the individual cells, thus limiting the development of many fields, such as biomedical. Nanofluidics based biochemical analysis exhibits advantages over conventional methods in terms of small sample volume, rapid turnaround time, straightforward operation, and efficient processing,which has been widely used in complex operations such as single cell capture, separation and single cell detection. Here we review the recent developments of nanofluidic technologies for single-cell analysis,with emphasis on cell trapping, treatment, and biochemical studies. The potential of nanofluidics-based single-cell analysis is discussed.

关键词： Nanofluidics Single cell Biochemical analysis Microfluidics

Zero-forcing beamforming with receiver antenna selection in downlink multi-antenna multi-user system

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Journal of Systems Engineering and Electronics 2008年第6期19卷 1258-1263页

作者： Huang Senhua Qiu ling Shi lin PCN & SS Lab Univ. of Science and Technology of China Hefei 230027 P. R. China

A study on the zero-forcing beamforming （ZFBF） scheme with antenna selection at user terminals in downlink multi-antenna multi-user systems is presented. Simulation results show that the proposed ZFBF scheme with receiver antenna selection （ZFBF-AS） achieves considerable throughput improvement over the ZFBF scheme with single receiver antenna. The results also show that, with multi-user diversity, the ZFBF-AS scheme approaches the throughput performance of the ZFBF scheme using all receiver antennas （ZFBF-WO-AS） when the base station adopts semi-orthogonal user selection （SUS） algorithm, and achieves larger throughput when the base station adopts the Round-robin scheduling algorithm. Compared with ZFBF-WO-AS, the proposed ZFBF-AS scheme can reduce the cost of user equipments and the channel state information requirement at the transmitter （CSIT） as well as the multiuser scheduling complexity at the transmitter.

关键词： downlink multi-antenna multi-user system zero-forcing beamforming antenna selection throughput performance

Intestinal microbiota and type 2 diabetes:from mechanism insights to therapeutic perspective

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World Journal of Gastroenterology 2014年第47期20卷 17737-17745页

作者： Jun-ling Han Hui-ling lin Department of Endocrinology and Metabolism the 187~(th) Hospital of PLA

The incidence of type 2 diabetes(T2DM)is rapidly increasing ***,the pathogenesis of T2DM has not yet been well *** evidence suggests that the intestinal microbiota composition is associated with obesity and *** this review,we provide an overview about the mechanisms underlying the role of intestinal microbiota in the pathogenesis of *** is clear evidence that the intestinal microbiota influences the host through its effect on body weight,bile acid metabolism,proinflammatory activity and insulin resistance,and modulation of gut *** gut microbiota with the use of probiotics,prebiotics,antibiotics,and fecal microbiota transplantation may have benefits for improvement in glucose metabolism and insulin resistance in the *** studies are required to increase our understanding of the complex interplay between intestinal microbiota and the host with *** studies may be able to boost the development of new effective therapeutic approaches for T2DM.

关键词： Intestinal microbiota Type 2 diabetes Body weight Bile-acid metabolism Insulin resistance Intestinal microbiota modulation

Interfacial phase competition induced Kondo-like effect inmanganite-insulator composites

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Frontiers of physics 2016年第6期11卷 129-133页

作者： ling-Fang lin ling-Zhi Wu Shuai Dong Department of Physics Southeast University Nanjing 211189 China School of Geography and Biological Information Nanjing University of Posts and Telecommunications Nanjing 210046 China

A Kondo-like effect, namely, the upturn of resistivity at low temperatures, is observed in perovskite manganite when nonmagnetic insulators are doped as secondary phase. In this paper, the low-temperature resistivity upturn effect has been argued to originate from interfacial magnetic phase reconstruction. Heisenberg spin lattices have been simulated using the Monte Carlo method to reveal phase competition around secondary phase boundary, namely, manganite-insulator boundary that behaves with a weak antiferromagnetic tendency. Moreover, the resistor network model based on double-exchange conductive mechanism reproduces the low-temperature resistivity upturn effect. Our work provides a reasonable physical mechanism to understand the novel transport behaviors in microstructures of correlated electron systems.

关键词： manganite Kondo-like effect manganite-insulator composites phase competition 75.47.Lx 75.47.Gk 73.63.-b

Key Questions on Reforming China’s Cultural Governance Capability in the Internet Era

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Frontiers of Literary Studies in China-Selected Publications from Chinese Universities 2020年第1期14卷 26-47页

作者： lin ling China Institute Fudan UniversityShanghai 200433China

In the past ten years,with the rise and popularity of Internet technology,Chinese society has experienced a silent“cultural uprising,”which has formed a part of the lives and habits of contemporary ordinary Chinese at different levels,and has challenged the old pattern of cultural power and *** to identify this phenomenon,and carry out corresponding reforms,as an important aspect of national governance,is an urgent research *** on the observation and analysis of the phenomenon of Chinese Internet culture in recent years,we can explore and summarize several key issues of China’s current cultural governance reform,and try to explain theoretically the influence of cultural prosperity on political power by retrospecting several governance experiences and lessons in *** the era of the new Internet technology,how to truly enhance the nation^basic ability to avoid the situation of dying once and letting *** complexity of contemporary cultural governance comes from the new technological conditions,while the difficult problem runs through the historical understanding of the entire new cultural governance capacity.

关键词： the Internet era “cultural uprising ”cultural governance ideology

Effects of electro-acupuncture on Noxa and caspase-3 expression in hippocampal CA1 region of a vascular dementia rat model

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Neural Regeneration Research 2008年第8期3卷 826-831页

作者： Yanzhen Zhu Qiang Wu ling lin Department of Integrative Medicine Fujian University of Traditional Chinese Medicine Fuzhou 350108 Fujian Province. China Department of Acupuncture and Moxibustion Fujian University of Traditional Chinese Medicine Fuzhou 350108 Fujian Province. China The Room for Confocal Laser Scanning Microscope Fujian Medical University Fuzhou 350004 Fujian Province China

BACKGROUND：Noxa, a pro-apoptotic member of the Bcl-2 protein family, has been shown to induce the mitochondrial pathway of apoptosis and to mediate hypoxic cell death in a rat model of cerebral ischemia. This suggests that Noxa could participate in apoptosis during vascular dementia （VD）. OBJECTIVE： To detect Noxa and caspase-3 expression after electro-acupuncture in VD rats to further validate the mechanism of electro-acupuncture-induced effects in the treatment of VD. DESIGN, TIME AND SETTING： A randomized, controlled study was performed at the Center for the Neurobiology of Fujian Medical University between January 2006 and March 2007. MATERIALS： A total of forty adult, male, Sprague Dawley rats were included in this study. The following equipment was used： confocal laser scanning microscope （Sp5, Leica, Germany）, water maze （Bejing Suntendy Science and Technology Co., Ltd., China）, and SDZ-II electronic acupuncture treatment instruments （Suzhou Medical Appliance Factory, China）. METHODS： Thirty-eight rats with sufficient learning and memory abilities were selected by Morris water maze criteria. Twelve rats received sham-surgery; the remaining 26 rats were used to establish a VD model by bilateral occlusion of the common carotid arteries. The rats that survived the occlusion procedure were randomly assigned into an electro-acupuncture group （n = 11） and a VD model group （n = 12）. MAIN OUTCOME MEASURES： Neuropathological changes were observed with hematoxylin-eosin staining of the hippocampus and expression of Noxa and caspase-3 in the hippocampal CA1 region was analyzed by confocal laser scanning microscope following immunofluorescence staining. RESULTS： Expressions of Noxa and caspase-3 in the electro-acupuncture group and sham-operated group were less than in the VD model group （P 〈 0.01）. Electro-acupuncture reduced the amount of apoptotic neurons in hippocampal CA1 area of rats with VD. The average latency in the Morris water maze test was significantly short

关键词： apoptosis caspase-3 electro-acupuncture Noxa vascular dementia

Relationship of PARG with PARP,VEGF and b-FGF in Colorectal Carcinoma

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Chinese Journal of Cancer Research 2009年第2期21卷 135-141页

作者： ling lin Jia Li Ya-lan Wang Xiao lin Department of Pathology Molecular Medicine and Cancer Research Center Chongqing Medical UniversityChongqing 400016 China

Objective： To investigate the relationship of poly（ADP-ribose）glycohydrolase（PARG） with poly （ADP-ribose） polymerase（PARP）, vascular endothelial growth factor （VEGF） and basic fibroblast growth factor（b-FGF） in colorectal carcinoma（CRC）. Methods： Immunohistochemical analysis was used to detect PARG, PARP, VEGF and b-FGF in human colorectal carcinoma. Flow cytometry was used to detect PARG and PARP in murine CT26 cell line. Gallotannin （GLTN） was served as PARG inhibitor. Results： The individual positive rates of PARG, PARE VEGF and b-FGF were 55.81%（24/43）, 97.67%（42/43）, 79.07%（34/43） and 81.40%（35/43）, respectively, which were significantly higher than those of control group. The positive PARG was correlated to PARP（r=0.3703, P〈0.05） and b-FGF （r=0.4838, P〈0.05）. The positive PARP was correlated to VEGF （r=0.3968, P〈0.05） and b-FGF （r=0.5610, P〈0.05）. Both PARG and PARP were expressed in CT26 cells. The positive staining rates of PARG and PARP in GLTN-treated group were 7.3% and 52.38%, respectively. They were markedly reduced than those of control group （55.41% and 95.28%, P〈0.01, n=10000）. Conclusion： The data suggest that PARG expression probably plays a role for VEGF and b-FGF expression in colorectal carcinoma.

关键词： PARG PARP VEGF b-FGF Colorectal carcinoma

Imitation of drug metabolism in cell co-culture microcapsule model using a microfluidic chip platform coupled to mass spectrometry

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Chinese Chemical Letters 2020年第2期31卷 451-454页

作者： Yajing Zheng Zengnan Wu Jin-Ming lin ling lin Beijing Key Laboratory of Microanalytical Methods and Instrumentation Department of ChemistryTsinghua UniversityBeijing 100084China State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBeijing 100029China CAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China

In this work,a multi-functional analysis platform by coupling a microfluidic chip to a mass spectrometry(MS) detector was *** constructed a three-dimensional tumor-endothelial co-culture model for simulating drug resistance during tumor *** this specially designed integrated platform,the first step was to prepare heterogeneous cell-encapsulated alginate microcapsules for threedimensional co-culture,and the second step was to achieve on-line perfusion culture and continuous drug stimulation on *** facilitates cell proliferation analysis and the collection of metabolism *** micro solid phase extraction column(SPE) pretreatment,subsequent mass spectrometry could detect drug *** high activity of two kinds of cells(A549 and HUVEC) shows the biocompatibility of the *** was used as a model drug,the distinctions of drug absorption between the mono-culture group and co-culture group were clearly observed by electrospray ionization quadrupole time-of-flight mass spectrometry(ESI-Q-TOF MS).Therefore,the integrated platform has shown promise as a high throughput,low cost for cell metabolism research and drug screening processes.

关键词： Microfluidic chip ESI-Q-TOF MS Co-culture Drug metabolism Droplet technique Hydrogel microcapsule

Lyricism as Epic＇s End： A New Approach to Wang Zengqi＇s Novels

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Frontiers of Literary Studies in China-Selected Publications from Chinese Universities 2016年第4期10卷 674-698页

作者： lin ling China Institute Fudan University Shanghai 200433 China

This article seeks to reinterpret Wang Zengqi＇s （汪曾祺, 1920-97） novels written in the early and mid-1980s. Through a historical lens, the author examines the era immediately following China＇s reform and opening-up when the political ideal of ＂distribution according to work＂（anlao fenpei） had met with social realities at that time. Departing from the mainstream approach to Wang Zengqi, which oversimplifies China＇s process of reforms and opening and consequently reduces Wang Zengqi＇s literature to a ＂pure literature＂ devoid of social implications, a lyricism of individuals, and a depiction of depoliticized everyday life, this article lays emphasis on the interconnection between Wang Zengqi＇s time and his writings. By analyzing the forms and styles of Wang＇s novels, the author endeavors to place his writings back in their historical context as a means of rediscovering their underlying meanings and politics hitherto neglected by the scholarship on Chinese literature from the 1980s. Therefore, this article refutes the misconception of Wang Zengqi being a ＂small writer＂ and acknowledges the writer＇s ＂bigness＂ in his writing. In fact, big writers like Wang Zengqi are indispensable in the conception of new political worlds under any historical condition.

关键词： reform and opening-up fenpei) China's early and mid-1980s realism "distribution according to work" (anlao lyrical style of writing realism socialist