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Molecular Diversity and Association Analysis of Drought and Salt Tolerance in Gossypium hirsutum L. Germplasm

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Journal of Integrative Agriculture 2014年第9期13卷 1845-1853页

作者： JIA Yin-hua SUN Jun-ling WANG Xi-wen ZHOU zhong-li PAN Zao-e HE Shou-pu PANG Bao-yin WANG Li-ru DU Xiong-ming State Key Laboratory of Cotton Biology Institute of Cotton Research Chinese Academy of Agricultural Sciences

Association mapping is a useful tool for the detection of genes selected during plant domestication based on their linkage disequilibrium（ld）. This study was carried out to estimate genetic diversity, population structure and the extent of ld to develop an association framework in order to identify genetic variations associated with drought and salt tolerance traits. 106 microsatellite marker primer pairs were used in 323 Gossypium hirsutum germplasms which were grown in the drought shed and salt pond for evaluation. Polymorphism（PIC=0.53） was found, and three groups were detected（K=3） with the second likelihood ΔK using STRUCTURE software. ld decay rates were estimated to be 13-15 cM at r2 0.20. Significant associations between polymorphic markers and drought and salt tolerance traits were observed using the general linear model（GLM） and mixed linear model（MLM）（P 0.01）. The results also demonstrated that association mapping within the population structure as well as stratification existing in cotton germplasm resources could complement and enhance quantitative trait loci（QTLs） information for marker-assisted selection.

关键词： cotton germplasm genetic diversity simple sequence repeats(SSR) markers linkage disequilibrium(ld) association analysis

Determination of the Photoperiod-Sensitive Inductive Phase in Maize with Leaf Numbers and Morphologies of Stem Apical Meristem

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Agricultural Sciences in China 2008年第5期7卷 554-560页

作者： WU Lian-cheng WANG Tie-gu KU Li-xia HUANG Qun-ce SUN Zhao-hui XIA zhong-liang CHEN Yan-hui Agronomy College Henan Agricultural University Zhengzhou 450002 P.R.China Henan Institute of Science and Technology Xinxiang 453003 P.R.China Henan Provincial Key Laboratory of lon Beam Bio-Engineering Zhengzhou University Zhengzhou 450052 P.R.China

It is vital to determine the effective photoperiods of maize for making full use of tropical germplasm, which is the foundation for determining the effect of latitude and planting date on the development of photoperiod-sensitive maize cultivars. The objective of this study is to determine the photoperiod-sensitive inductive phase using reciprocal transfer between long- day （ld）（15 h d^-1） and short-day conditions （SD）（9 h d^-1）. For Huangzao 4 and CML288, days to tassel and pollen shedding were recorded, and stem apical meristems （SAM） were observed by a laser scanning confocal microscope. The results show that the seedlings are insensitive to photoperiod when they are very young （juvenile）. However, after this period, ld delays flowering and increases the leaf numbers below the inflorescence, and the length of the interval of the photoperiod-sensitive inductive phase is longer under ld conditions than under SD conditions. Transferred from SD to ld, plants show a sudden decrease in leaf numbers once sufficient SD has been received for flower commitment. While transferred from ld to SD, plants have a continuous increase in leaf numbers during the photoperiod sensitive inductive phase under ld conditions. At the same time, when plants are competent to flowers, the obvious morphology is the elongation of maize SAM. There is an obvious variance of the photoperiod sensitive phase under ld and SD conditions in different maize.

关键词： maize photoperiod flowering LSCM stem apical meristem (SAM) new full expansion leaf

Construction of EMS-Induced Peanut Mutant Libraries and Identification of Pod-Related Traits Mutant Lines

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 537-557页

作者： Hao Chen Faqian Xiong Rilong Xu Xiangyu Chen Haifeng zhong Yumei Zhang Xinlong Lan Hong Zhang Yuhua Chen Runfang Hu Guoqiang Lin Zhaoxiu Tang Institute of Crop Sciences Fujian Academy of Agricultural SciencesFujian Research Station of Crop Gene Resource&Germplasm EnhancementMinistry of Agriculture and Rural Affairs of People’s Republic of ChinaFujian Engineering Research Center for Characteristic Upland Crops BreedingFujian Engineering Laboratory of Crop Molecular BreedingFuzhou350013China Cash Crops Research Institute Guangxi Academy of Agricultural SciencesNanning530007China

Peanut(Arachis hypogaea L.)is an oil and economic crop of vital importance,and peanut pod is the key organ influencing the yield and processing ***,the Pod-related traits(PRTs)are considered as important agronomic traits in peanut *** broaden the variability of PRTs in current peanut germplasms,three elite peanut cultivars were used to construct Ethyl methane sulfonate(EMS)-induced mutant libraries in this *** optimal EMS treatment conditions for the three peanut varieties were *** was found that the median lethal dose(ld50)of EMS treatment varied greatly among different ***,the EMS-induced peanut mutant libraries were constructed and a total of 124 mutant lines for PRTs were identified and ***,“M-8070”,one of the mutant lines for pod constriction,was re-sequenced via high-throughput sequencing *** genome-wide variations between“M-8070”and its wild parent“Fuhua 8”(FH 8)were detected.2994 EMS-induced single nucleotide polymorphisms(SNPs)and 1188 insertion-deletions(InDels)between“M-8070”and its wild parent were *** predominant SNP mutation type was C/G to T/A transitions,while the predominant InDel mutation type was“1-bp”.We analyzed the distribution of identified mutations and annotated their *** of the mutations(91.68%of the SNPs and 77.69%of the InDels)were located in the intergenic region.72 SNPs were identified in the exonic region,leading to 27 synonymous,43 nonsynonymous and 2 stop-gain variation for gene structure.13 Indels were identified in the exonic region,leading to 4 frame-shift,8 non-frame-shift and 1 stop-gain variations of *** mutations may lead to the phenotypic variation of“M-8070”.Our study provided valuable resources for peanut improvement and functional genomic research.

关键词： Peanut EMS-induced mutant line pod-related traits re-sequencing pod constriction

Vegetarian diets,circulating miRNA expression and healthspan in subjects living in the Blue Zone

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Precision Clinical Medicine 2020年第4期3卷 245-259页

作者： Tiantian Liu Nicole M.Gatto zhong Chen Hongyu Qiu Grace Lee Penelope Duerksen-Hughes Gary Fraser Charles Wang Center for Genomics School of MedicineLoma Linda UniversityLoma LindaCA 92350USA School of Community and Global Health Claremont Graduate UniversityClaremontCA 91711USA Center of Molecular and Translational Medicine Institution of Biomedical ScienceGeorgia State UniversityAtlantaGA 30303USA Department of Psychology School of Behavioral HealthLoma Linda UniversityLoma LindaCA 92350USA Department of Basic Sciences School of MedicineLoma Linda UniversityLoma LindaCA 92350USA School of Public Health Loma Linda UniversityLoma LindaCA 92350USA

A long-term vegetarian diet plays a role in the longevity and maintenance of the healthspan,but the underlying mechanisms for these observations are largely ***,it is not known whether a longterm vegetarian dietary pattern may affect the circulating miRNA expression in such a way as to modulate the *** Adventist Health Study-2(AHS-2)cohort includes a large number of older adults who primarily follow vegetarian dietary patterns and reside in Loma linda,California,one of five“Blue Zones”in the world in which a higher proportion of the population enjoys a longer than average *** performedmiRNA-seq in 96 subjects selected from the AHS-2 cohort with different dietary *** identified several differentially expressed miRNAs between vegetarians and non-vegetarians,which are involved in immune response and cytokine signaling,cell growth and proliferation as well as age-related diseases such as cardiovascular diseases and neurodegenerative ***,our study showed that a vegetarian diet modulates aging-associated circulating miRNAs in a sex-dependent manner of differential expression for certain miRNAs,which may be related in a beneficial manner to the *** investigation is needed to validate these miRNAs as potential biomarkers for diet-modulated longevity in humans.

关键词： circulating miRNA aging miRNA sequencing vegetarian diet Blue Zone

HBGH患者血清CGN、SDC-1水平与病情及疾病转归的关系

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国际检验医学杂志 2024年第10期45卷 1238-1242页

作者：朱贤龙明圆圆申潇竹邵世珂仲崇佩樊拥军董文胜连云港市第二人民医院神经外科江苏连云港222002 连云港市第一人民医院神经外科江苏连云港222000

目的探讨血清扣带蛋白(CGN)、多配体聚糖-1(SDC-1)水平与高血压性基底节脑出血(HBGH)患者病情及疾病转归的关系。方法选取2019年2月至2022年2月连云港市第二人民医院(下称该院)收治的123例HBGH患者为研究对象,另选择该院同期行健康体... 详细信息

目的探讨血清扣带蛋白(CGN)、多配体聚糖-1(SDC-1)水平与高血压性基底节脑出血(HBGH)患者病情及疾病转归的关系。方法选取2019年2月至2022年2月连云港市第二人民医院(下称该院)收治的123例HBGH患者为研究对象,另选择该院同期行健康体检的体检健康者120例为健康组。检测两组纳入对象血清CGN、SDC-1表达水平。根据疾病转归情况将患者分为好转组92例,恶化组31例。采用受试者工作特征(ROC)曲线及曲线下面积(AUC)分析血清CGN、SDC-1表达水平对HBGH患者疾病转归的预测价值。结果重度组血清CGN、SDC-1表达水平均高于中度组、轻度组,且中度组血清CGN、SDC-1表达水平均高于轻度组,差异有统计学意义(P<0.05),3组HBGH患者血清CGN、SDC-1表达水平均高于健康组,差异有统计学意义(P<0.05)。恶化组血清CGN、SDC-1表达水平高于好转组,差异有统计学意义(P<0.05)。血清CGN、SDC-1预测HBGH患者疾病转归的AUC分别为0.742(95%CI:0.792~0.697)、0.861(95%CI:0.906~0.910),两者联合预测的AUC为0.917(95%CI:0.962~0.870)。恶化组出血量、破入脑室情况明显高于好转组,入院格拉斯哥昏迷量表(GCS)评分低于好转组,差异有统计学意义(P<0.05)。多因素Logistic回归分析显示,血清CGN≥51.63 pg/mL(OR=3.815)、血清SDC-1≥450.67μg/L(OR=4.230)、入院GCS评分≤8分(OR=5.333)是HBGH患者疾病转归的影响因素(P<0.05)。结论血清CGN、SDC-1表达水平升高与HBGH患者病情加重、疾病转归恶化密切相关,二者对HBGH患者疾病转归具有一定预测价值。

关键词：扣带蛋白多配体聚糖-1 高血压基底节脑出血疾病转归

Y_2O_2S∶Er,Yb纳米晶的制备及其上转换和下转换发光

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功能材料 2009年第6期40卷 896-899页

作者：仲海洋曹望和大连海事大学物理系辽宁大连116026

采用共沉淀法,结合固-气硫化工艺制备了平均粒径约40nm的类球形Y2O2S∶Er,Yb纳米晶,并对其在980nm红外激发下的上转换和下转换发射进行了较为详细的研究。Er3+的上转换特征发射分别位于530、550和660nm附近。在表面吸附OH-和CO32-所形... 详细信息

采用共沉淀法,结合固-气硫化工艺制备了平均粒径约40nm的类球形Y2O2S∶Er,Yb纳米晶,并对其在980nm红外激发下的上转换和下转换发射进行了较为详细的研究。Er3+的上转换特征发射分别位于530、550和660nm附近。在表面吸附OH-和CO32-所形成的特殊声子环境下,Er3+的4S3/2→4F9/2和4I11/2→4I13/2多声子弛豫显著提高了4F9/2能级的粒子布居,致使纳米晶具有很强的660nm红光发射。并且红绿光荧光分支比会随Yb3+浓度的提高而显著增大。首次报导了Y2O2S纳米晶中Er3+的红外特征发射。源于Er3+离子4I13/2→4I15/2辐射跃迁的较强红外发射,在晶场作用下分化为峰位分别位于1501、1534和1577nm的3个发射峰。由于4I13/2能级是660nm红光发射的主要布居能级之一,该红外发射与红光发射存在明显竞争。但在较高Yb3+掺杂浓度下,高效的Yb3+→Er3+能量传递可有效削弱红外发射的竞争作用。

关键词： YzO2S:Er,Yb纳米晶上转换下转换发光

Carbon materials for metal-ion batteries

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ChemPhysMater 2023年第4期2卷 267-281页

作者： zhong Qiu Feng Cao Guoxiang Pan Chen Li Minghua Chen Yongqi Zhang Xinping He Yang Xia Xinhui Xia Wenkui Zhang College of Materials Science&Engineering Zhejiang University of TechnologyHangzhou 310014China Department of Engineering Technology Huzhou CollegeHuzhou 313000China Department of Materials Engineering Huzhou UniversityHuzhou 313000China Institute of Fundamental and Frontier Science University of Electronic Science and Technology of ChinaChengdu 611371China Key Laboratory of Engineering Dielectric and Applications(Ministry of Education) School of Electrical and Electronic EngineeringHarbin University of Science and TechnologyHarbin 150080China State Key Laboratory of Silicon Materials Key Laboratory of Advanced Materials and Applications for Batteries of Zhejiang ProvinceDepartment of Materials Science and EngineeringZhejiang UniversityHangzhou 310027China

Metal-ion(Li-,Na-,Zn-,K-,Mg-,and Al-ion)batteries(MIBs)play an important role in realizing the goals of“emission peak and carbon neutralization”because of their green production techniques,lower pollution,high voltage,and large energy ***-based materials are indispensable for developing MIBs and are widely adopted as active or auxiliary materials in the anodes and *** example,carbon-based materials,includ-ing graphite,Si/C and hard carbon,have been used as anode materials for Li-and Na-ion *** can also be used as a conductive coating for cathodes,such as in LiFePO 4/C,to achieve better *** addition,as new high-valence MIBs(Zn-,Al-,and Mg-ion)have emerged,a growing number of novel carbon-based mate-rials have been utilized to construct high-performance ***,we discuss the recent development trends in advanced carbon-based materials for *** impact of the structure properties of advanced carbon-based materials on energy storage is addressed,and a perspective on their development is also proposed.

关键词： Carbon materials Electrochemical energy storage Metal-ion batteries Lithium-ion batteries Sodium-ion batteries

Pr3+/Nd3+ codoped tellurite glass for O+E+S-band broad emission

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Optoelectronics Letters 2019年第6期15卷 424-427页

作者： SHEN Xin-jie ZHU Ya-rui ZHOU Zi-zhong SU Xiu-e ZHOU Ming-han ZHOU Ya-xun College of Information Science and Engineering Ningbo University

In this work, Pr3+ was introduced into Nd3+-doped tellurite glass with composition Te O2-Zn O-Na2 O-Nb2 O5 to achieve broadband near-infrared emission. A broadband fluorescence emission ranging from 1 250 nm to 1 530 nm was obtained under the excitation of 808 nm ld, which is contributed by the Pr3+:1 D2 →1 G4 and Nd3+:4 F3/2→4 I13/2 transitions emitting the fluorescence located at around 1.47 μm and 1.35 μm bands, respectively. The 1.47 μm band fluorescence of Pr3+ is attributed to the energy transfer from Nd3+ to Pr3+ ions and the energy transfer mechanism was further investigated by calculating relevant micro-parameter and phonon contribution ratio. Meanwhile, the studied tellurite glass possesses good thermal stability. The present work indicates that Pr3+/Nd3+ codoped tellurite glass is an excellent gain medium for potential O+E+S-band broad optical amplifiers.

关键词： tellurite glass band

Upconversion luminescent property and EPR study of NaGdF4：yb3＋/Tm3＋ synthesized by the hydrothermal method

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Frontiers of Materials Science 2015年第3期9卷 241-246页

作者： Jing LIU Jing-Ying ZHANG Kai LIU Hong-Jian GAO Xiao-Long YU Yang CAO zhong-Xin LIU Key Laboratory of Ministry of Education for Advanced Materials in Tropical Island Resources Hainan University Haikou 570228 China

Water soluble upconversion （UC） luminescence hexagonal-phase NaGdF4： yb3＋/Tm3＋ nanoparticles have been successfully synthesized by the hydrothermal method. XRD, SEM, UC photoluminescence spectra and electron paramagnetic resonance （EPR） spectrum were used to characterize the nanoparticles. The intensity of UC emission region could be controlled through different sodium source and the fluorine source, 6PJ→8S7/2 emission of Gd3＋ is also observed at 310 nm. A broad spectrum with a dominant resonance at g of about 2 was observed by the EPR spectrum of the NaGdF4：Yb3＋/Tm3＋ nanoparticles. The transparent NaGdF4：yb3＋/Tm3＋ solution presented naked eye-visible violet-blue light under the 980 nm ld excitation. The current work paves the way for their potential application in infrared tomography and magnetic resonance imaging （MRI）.

关键词： hydrothermal method sodium source fluorine source electron paramagnetic resonance (EPR) magnetic resonance imaging (MRI)