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The effects of selenium on the translation efficiency of selenoproteins Gpx1， Gpx4， and Txnrd1 in oxLDL-induced ***926 cells

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Food Science and Human Wellness 2024年

作者： Jiaqi Lu Jing Meng Yanzhi Lang Abdul Rehman Yamei Yan linwu Ran School of Public Health， Ningxia Medical University Key Laboratory of Environmental Factors and Chronic Disease Control Yinchuan School of Clinical Medicine， Ningxia Medical University Goji Berry Research Institute， Ningxia Academy of Agriculture and Forestry Sciences

Selenium is a crucial trace element that contributes to physiological processes in the body as selenoproteins. Selenoproteins serve as an integral role in the body in controlling the redox state of cells and protecting against damage induced by oxidative stress. This study aimed to investigate the effects and possible mechanism of selenium on selenoproteins expression in ***926 cells induced by oxLDL. The impact of selenium on the viability of ***926 cells was detected by the MTT method， and intracellular ROS level and mitochondrial membrane potential were assessed by fluorescent probe DCFH-DA and JC-1， respectively. RNA-seq， quantitative real-time PCR (RT-qPCR)， and western blot were used to investigate the selenoprotein expression. Selenoprotein mRNA translation efficiency was analyzed by ribosome profiling (Ribo-Seq) coupled with transcriptomics. Our data showed that selenium supplementation (0.5 μmol/L) significantly decreased ROS production， reduced mitochondrial inner membrane potential and increased the proliferative activity of ***926 cells induced by oxLDL. Moreover， The protective effects of selenium against oxLDL-induced ***926 cell injury were associated with the upregulation of the expressions of selenoproteins GPX1， GPX4， and TXNRD1. Furthermore， the expressions of selenoproteins GPX1 and GPX4 were hierarchically controlled， but the expressions of selenoproteins TXNRD1 were mainly regulated by oxLDL. Finally， Ribo-Seq coupled with transcriptomics results demonstrated that the expressions of selenoproteins GPX1， GPX4， and TXNRD1 were regulated at the translation process level. These findings suggested that selenium could have preventive effects in oxLDL induced ***926 cell injury by regulating the selenoprotein expression， and the selenoproteins expressions at the translation level in vascular endothelial cells need further study.

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Development and evaluation of an online monitoring single-particle optical particle counter with polarization detection

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Journal of Environmental Sciences 2024年第4期138卷 585-596页

作者： Weijie Yao Xiaole Pan Yu Tian Hang Liu Yuting Zhang Shandong Lei Junbo Zhang Yinzhou Zhang linwu Yele Sun Zifa Wang State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China Center for Excellence in Regional Atmospheric Environment Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China

We developed a single-particle optical particle counter with polarization detection(SOPC)for the real-time measurement of the optical size and depolarization ratio(defined as the ratio of the vertical component to the parallel component of backward scattering)of atmospheric particles,the polarization ratio(DR)value can reflect the irregularity of the *** SOPC can detect aerosol particles with size larger than 500 nm and the maximum particle count rate reaches~1.8×10^(5)particles per *** SOPC uses a modulated polarization laser to measure the optical size of particles according to forward scattering signal and the DR value of the particles by backward S and P signal *** sampling rate of the SOPC was 106#/(sec·channel),and all the raw data were processed *** calibration curve was obtained by polystyrene latex spheres with sizes of 0.5-10μm,and the average relative deviation of measurement was 3.96% for sub 3μm particles.T-matrix method calculations showed that the DR value of backscatter light at 120°could describe the variations in the aspect ratio of particles in the above size *** performed insitu observations for the evaluation of the SOPC,the mass concentration constructed by the SOPC showed good agreement with the PM_(2.5)measurements in a nearby state-controlled monitoring *** instrument could provide useful data for source appointment and regulations against air pollution.

关键词： Instrument development Single particle detection Measurement uncertainty Optical simulation Mass concentration

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加强水污染治理和水环境监测的几点思考

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科技资讯 2024年第6期22卷 179-181页

作者：梅林武遵义市生态环境污染防治中心贵州遵义563200

随着工业化、城市化的快速发展,水资源的污染问题日益凸显,对生态环境和人类健康构成了严重威胁。为了有效应对这一挑战,加强水污染治理和水环境监测已成为当务之急。基于此,应针对当前水污染治理和水环境监测的现状进行分析,并提出了... 详细信息

随着工业化、城市化的快速发展,水资源的污染问题日益凸显,对生态环境和人类健康构成了严重威胁。为了有效应对这一挑战,加强水污染治理和水环境监测已成为当务之急。基于此,应针对当前水污染治理和水环境监测的现状进行分析,并提出了几点思考。期望通过此次研究能够全面提升水污染治理和水环境监测的水平和效果,为创造健康的水生态环境提供有力保障。

关键词：水污染治理技术水环境监测生态环境

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水环境保护工程中的生态修复治理技术

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科技资讯 2024年第7期22卷 200-202页

作者：梅林武遵义市生态环境污染防治中心贵州遵义563200

随着工业化和城市化的快速推进,水环境污染问题日趋严重,传统的物理、化学治理方法已经难以满足现实需求。在此背景下,生态修复治理技术作为一种环境友好的解决策略,逐渐被广泛应用在水环境保护工程中。基于此,通过对生态修复治理技术... 详细信息

随着工业化和城市化的快速推进,水环境污染问题日趋严重,传统的物理、化学治理方法已经难以满足现实需求。在此背景下,生态修复治理技术作为一种环境友好的解决策略,逐渐被广泛应用在水环境保护工程中。基于此,通过对生态修复治理技术的分析,探讨了其在水环境保护工程中的应用与实践,以期为我国水环境保护工作提供理论指导和参考。

关键词：水环境保护生态修复物理修复化学修复

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Causes and Consequences of Changes in Nutrient Structure in the Jiaozhou Bay

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Journal of Integrative Plant Biology 2005年第4期47卷 396-410页

作者： Shu-JiangZHAO Nian-ZhiJIAO Zhi-LiangSHEN Yu-linwu InstituteofOceanography theChineseAcademyofSciencesQingdao266071China／／QingdaoMarineEngineeringProspectingInstituteStateOceanAdministrationQingdao266033China MinistryofEducationKeyLaboratoryforMarineEnvironmentalScience XiamenUniversityXiamen361005China InstituteofOceanography theChineseAcademyofSciencesQingdao266071China

: Concentrations and ratios of nutrients in Jiaozhou Bay, China, have changed much in the past decades, with trends indicating an increase in nitrogen and a decrease in silicate. Statistical analysis has shown that the long-term variations of nutrients are associated with agricultural activities, precipitation, and anthropogenic factors. Stoichiometric calculations indicate that the nutrient structure has become more and more unbalanced. There has been almost no possibility for nitrogen limitation since the 1980s, the probability of P limitation has increased, and the probability of Si limitation has also increased markedly from the 1980s to the 1990s. As a consequence of changes in nutrient structure, a decrease in the abundance of net phytoplankton was evident, whereas total chlorophyll a, levels have remained roughly unchanged at around 3.55 g/L. Thus, it is likely that smaller species have taken the niche vacated by the larger species. Changes in phytoplankton size and species composition may ultimately lead to various functional and structural changes at the system level.

关键词： anthropogenic factor diatoms Jiaozhou Bay nutrient structure silicon limitation stoichio metric calculations

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Simulation and analysis of the carrying capacity for road networks using a grid-based approach

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Journal of Traffic and Transportation Engineering(English Edition) 2020年第4期7卷 498-506页

作者： Junqing Shi linwu Chen Fengxiang Qiao Lei Yu Qing Li Guilian Fan College of Engineering Zhejiang Normal UniversityJinhua 321004China Innovative Transportation Research Institute Texas Southern UniversityHoustonTX 77004USA Xuchang University Xuchang 461000China Beijing Jiaotong University Beijing 100044China Wuhan Transportation Science Research Institute Wuhan 430015China

The number of vehicles that a road network can carry is *** the limit is exceeded,the network system is not able to function *** this paper,an updated cellular automaton model for grid networks with all-way stop-controlled intersections is proposed to simulate the network level critical density and carrying capacity under different conditions,which essentially indicates the limit number of vehicles that the network can handle before going into *** the proposed model,two update rules,including lane-changing and the longitudinal location update,are adopted to describe the vehicle’s movements on road segments according to the driving condition on the road and the vehicle’s direction in the downstream *** vehicle’s movements in intersection areas are prioritized based on vehicle’s position,so as to prevent collisions within the intersection *** simulation results show that an increase in network size is able to expand the carrying capacity of a road network,whereas the expansion rate is lower than the change rate in the network *** carrying capacity is also associated with the structure of road *** carrying capacity is inversely proportional to the number of intersections,and proportional to the length of the road in the ***,optimizing the origin-destination(O-D)distribution can increase the carrying capacity of an urban road network.

关键词： Transportation Grid road network Carrying capacity Congestion Critical density Cellular automata model

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Poplar Trees （Populus canadensis Moench） Initiate Vegetative Storage Protein Accumulation During New Shoot Development in Spring

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Journal of Integrative Plant Biology 2005年第6期47卷 717-725页

作者： Wei-MinTIAN Shi-QingPENG Bing-ZhongHAO Ji-linwu Zheng-HaiHU Ke-MingCUI CollegeofLifeSciences PekingUniversityBeijing100871China HainanProvincialKeyLaboratoryofCultivationPhysiologyforTropicalCrops RubberResearchInstituteChineseAcademyofTropicalAgriculturalSciencesDanzhou571737China NationalKeyBiotechnologyLaboratoryforTropicalCrops ChineseAcademyofTropicalAgriculturalSciencesHaikou571101China InstituteofBotany NorthwestUniversityXi＇an710069China

: It was generally assumed that the accumulation of vegetative storage protein (VSP) in poplar trees and/or temperate hardwoods did not occur in spring. To test this assumption, the accumulation of the 32-kDa VSP and the differential expression of a gene encoding for the protein in poplars were investigated using light and electron microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and reverse transcription-polymerase chain reaction (RT-PCR). We report, for the first time, that poplar trees initiated VSP accumulation in new shoots during the development of new shoots in spring under conditions of high temperature and long days. The amount of 32-kDa VSP increased gradually in the stem of new shoots and in two-year-old branches, but there were no detectable changes in its abundance in the bark tissues of trunks during new shoot development. Based on the presence of a 286-bp DNA fragment that is identical to the VSP gene bspA, encoding for the 32-kDa VSP in Populus deltoids Bartr. ex Marsh., the differential expression of the 32-kDa VSP gene in P. canadensis Moench was revealed by RT-PCR. The results indicated that the 32-kDa VSP gene was expressed strongly in new shoots, relative weakly in two-year-old branches and was not expressed in the trunk during new shoot development. This pattern of VSP accumulation and VSP gene space-time differential expression may be an important mechanism by which stored nitrogen compounds are used preferentially to exogenously available nitrogen and, in addition, the dynamic pattern may also have a role in the regulation of nitrogen metabolism, especially nitrogen uptake by the roots.

关键词： new shoot nitrogen storage Populus canadensis temperate hardwoods vegetative storage proteins

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四川省某卷烟厂中央空调系统节能研究

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制冷与空调（四川） 2024年第3期38卷 410-415页

作者：肖政陶竞立陈利杨宗成胡茂凌王林武四川中烟工业有限责任公司成都卷烟厂成都610066

近年来我国烟草产量逐年增加,但同时该行业能源消耗较大,因此对卷烟企业进行节能改造,提高企业的综合生产能耗水平成为了各地企业的重要任务。以四川省某卷烟厂为研究对象,首先结合相关标准规范对厂房的生产水平进行评估,结果表明企业... 详细信息

近年来我国烟草产量逐年增加,但同时该行业能源消耗较大,因此对卷烟企业进行节能改造,提高企业的综合生产能耗水平成为了各地企业的重要任务。以四川省某卷烟厂为研究对象,首先结合相关标准规范对厂房的生产水平进行评估,结果表明企业综合产值较低,而厂房中央空调系统能耗占据企业生产用能的70%左右,因此对该系统进行节能降耗有重要意义。对夏、冬季中央空调系统运行性能分析,发现冷水机组、冷冻水泵以及冷却水泵的开启策略以及设定参数均不合理;最终对设备的运行台数及设定参数给出优化建议,计算出夏、冬季中央空调系统可节省684200kWh的电耗。

关键词：卷烟厂中央空调系统节能降耗运行性能

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多重连接探针扩增法检测和识别脊柱肌肉萎缩症的纯合型或杂合型SMN基因缺失(英文)

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北京大学学报（医学版） 2005年第1期37卷 55-57页

作者： KeithTOMASZEWICZ PeterKANG Bai-linwu DepartmentsofLaboratoryMedicine／／DepartmentofPathology DepartmentsofLaboratoryMedicine DepartmentsofNeurology Children＇sHospitalandHarvardMedicalSchool-BostonMA02115USA

Objective: Spinal muscular atrophy(SMA), an autosomal recessive neuromuscular degeneration of the anterior horn cells of the spinal cord and brain stem, results in one of the most common diseases with muscle fatigue and atrophy. Most SMA cases including all the types are due to the homozygous deletion of at least exon 7 within the survival motor neuron 1 (SMN-1) gene. Although a “golden standard” assay (PCR with mismatch primer followed by enzyme digestion) is very reliable for the identification of homozygous SMN-1 deletion, the carrier detection of heterozygous SMN-1 deletion remains a challenge. Methods: Some PCR-based gene dosage assays or multiplex PCR allow for the determination of the copy number of SMN-1 gene to identify heterozygous deletion, but these procedures are often time consuming and available on a limited clinical basis. Recently developed MLPA (multiplex ligation-dependent probe amplification) is an efficient procedure that can accurately analyze relative quantification to establish the copy number of the SMN gene. We performed a validation for simultaneous detection of homozygous SMN-1 deletions of SMA patients and heterozygous SMN-1 deletions of SMA carriers in a simple assay using a MLPA-SMA assay specific reagent. Results: Six out of 20 patients with SMA were found to have homozygous SMN-1 deletion, confirmed by the PCR/digestion assay. All 4 parents of the children with SMA had heterozygous SMN-1 deletion, confirmed by an independent relative quantitative analysis. Conclusion: MLPA provides a simple, rapid and accurate method of simultaneously detecting homozygous deletions and heterozygous deletions in a single assay for both SMN-1 and SMN-2 genes.

关键词：肌萎缩脊髓性基因 SMN 多重连接探针扩增法基因缺失业杂合子检测

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Specific growth inhibition by alteration of metabolic pathway using Chinese herbal medicine in tyrosine kinase inhibitor resistant non-small cell lung cancer

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中国药理学与毒理学杂志 2015年第S1期29卷 85-86页

作者： Xing-xingFAN MariaPikWONG Zhi-weiCAO Jian-linwu HuaZHOU Zhi-hongJIANG LiangLIU ElaineLai-hanLEUNG State Key Laboratory of Quality Research in Chinese Medicine Macao Institute For Applied Research in Medicine and Health Macao University of Science and Technology Macao China Department of Pathology Li Ka Shing Faculty of Medicine University of Hong Kong Hong Kong China School of Life Science Tongji University Shanghai China

OBJECTIVE Lung cancer is the leading cause of cancer death *** growth factor receptor(EGFR)mutation(s)is/are common in non-small cell lung cancer(NSCLC)in Asian population,resulting in lung tumor formation.L858 Rsubstitution mutation on exon 21 and in-flame deletion mutation on exon 19 are the two most common forms of EGFR *** targeted therapy using tyrosine kinase inhibitor(TKI)targeting EGFR shows promising initial response,however drug resistance is ***,it is needed to identify new inhibitors to tackle *** this study,we aim to investigate the effect of multiple single purified compounds derived from Chinese herbal medicines(CMHs)on a panel of NSCLC cell lines with different EGFR mutational statuses and TKI *** also examine the biological functional effect and drug action mechanism of these cell lines after drug *** We have reviewed the literature and selected ten single purified compounds derived from CMHs which exhibited the highest potential of cancer suppression effect in *** have recruited three EGFR-dependent NSCLC cell lines for drug screening using cytotoxicity assay.A549 is used as EGFR wild-type *** TKI-resistant NSCLC cell lines were used,H1975 harbors double mutation(EGFRL858R+T790M)and H1650 harbors EGFRexon 19 deletion.H2228is a NSCLC cell line which harbours EML4-ALK fusion gene and was used as EGFR-independent cell line *** assay was used to determine the drug efficacy and IC50 *** functional assays including cell cycle arrest analysis and apoptosis assay was used to determine the biological effect after drug *** MTT assay revealed that six out of ten candidate agents showed significant cancer-inhibiting effects in H1650 and *** compounds exhibited IC50 value at micro-molar levels while another three compounds exhibited IC50 at as low as nano-molar *** compound exhibited specificity on EGFR-dependent NSCLC

关键词： non-small cell lung cancer EGFR TKI resistance,can

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