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Chinese Medical Journal 2010年第8期123卷 1073-1077页

作者： YANG Jing-lin wang hong-liang wang Shun-xin YANG Peng LIU Kang-tai JIANG Cheng-yu National Key Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100005 China

Background Numerous Asian cases of avian influenza virus infection, especially the highly pathogenic strain H5N1, in humans have raised the concern that another influenza pandemic is close. However, there are no effective therapeutic drugs or preventative vaccines available. Hemagglutinin is the membrane glycoprotein of avian influenza virus responsible for receptor binding to human cells and the main immunogenic protein that elicits a strong immune response. Although this protein is of great importance to the study of pathogenesis and vaccine development, its expression and purification are difficult due to high levels of glycosylation. Methods In this study, we expressed codon-optimized, full-length hemagglutinin 5 （H5） protein fused with a human IgG Fc tag （H5-Fc） in HEK293 cells. To enhance secretion of this protein, we also deleted the transmembrane domain and the intracellular domain of the H5 protein （H5ATM-Fc）. Purified proteins were obtained using a protein A column. Results ELISA revealed that the yield of soluble H5ATM-Fc protein in the supernatant was about 20 mg/L. Western blotting and fluorescence activated cell sorter （FACS） indicated that the purified H5 protein was correctly folded and biologically active. Conclusion Purification of H5 proteins from mammalian cells could be used for large-scale production of recombinant H5 protein for basic scientific research or the development of vaccines.

关键词： hemagglutinin avian influenza protein purification mammalian cell expression

Exact Solutions to Extended Nonlinear SchrSdinger Equation in Monomode Optical Fiber

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Communications in Theoretical Physics 2006年第1期 131-134页

作者： BAI Cheng-lin ZHAO hong wang Wei-Tao Physical Science and Information Engineering School,Liaocheng University,Liaocheng 252059,China

By using the generally projective Riccati equation method,more new exact travelling wave solutionsto extended nonlinear Schr■dinger equation(NLSE),which describes the femtosecond pulse propagation in monomodeoptical fiber,are found,which include bright soliton solution,dark soliton solution,new solitary waves,periodic solutions,and rational *** finding of abundant solution structures for extended NLSE helps to study the movementrule of femtosecond pulse propagation in monomode optical fiber.

关键词： extended NLSE generally projective Riccati equation method soliton solutions optical fiber

RF Optimizer Model for Predicting Compressive Strength of Recycled Concrete

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 215-223页

作者： LIU lin wang Liuyan wang Hui SUN Huayue School of Civil Engineering and Architecture Shenyang UniversityShenyang 110000China

Traditional machine learning(ML)encounters the challenge of parameter adjustment when predicting the compressive strength of reclaimed *** address this issue,we introduce two optimized hybrid models:the Bayesian optimization model(B-RF)and the optimal model(Stacking model).These models are applied to a data set comprising 438 observations with five input variables,with the aim of predicting the compressive strength of reclaimed ***,we evaluate the performance of the optimized models in comparison to traditional machine learning models,such as support vector regression(SVR),decision tree(DT),and random forest(RF).The results reveal that the Stacking model exhibits superior predictive performance,with evaluation indices including R2=0.825,MAE=2.818 and MSE=14.265,surpassing the traditional ***,we also performed a characteristic importance analysis on the input variables,and we concluded that cement had the greatest influence on the compressive strength of reclaimed concrete,followed by ***,the Stacking model can be recommended as a compressive strength prediction tool to partially replace laboratory compressive strength testing,resulting in time and cost savings.

关键词： machine learning recycled concrete compressive strength

Turbulent Agglomeration of Microparticles in a Cylinder Wake Flow Using LES-DEM:Focusing on the Effect of the Reynolds Number

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Journal of Thermal Science 2025年第1期34卷 34-49页

作者： wang Shuang MU lin LI Xue XIE Jun DONG Ming School of Energy and Power Engineering Dalian University of Technology School of Optical Information and Energy Engineering Wuhan Institute of Technology School of Energy and Environment Shenyang Aerospace University

Turbulent agglomeration is viewed as a promising technology for enhancing fine particle removal *** better understand particle transport,agglomeration behaviors,and fluid-particle interactions,we numerically explored these phenomena under cylindrical vortex wake influence using a coupled large eddy simulation and discrete element method（LES-DEM） *** validity of the LES approach was verified by comparison with available direct numerical simulation（DNS） *** adopted the Johnson-Kendall-Roberts（JKR） contact model for particle-particle *** particle dispersion and agglomeration characteristics of particles with different diameters（dp=2-20 μm） in the laminar and transition of shear layer（TrSL） flow regimes were *** particles were concentrated at the vortex centers,while larger particles accumulated around the *** agglomeration efficiency exhibited an M-shaped profile spanwise（y-direction）.With increasing Reynolds number,the agglomeration efficiency and turbulence intensity *** particle agglomeration efficiency peaks at a certain Reynolds ***,at higher Reynolds numbers,reducing the residence time of particles in the flow field decreases the agglomeration efficiency.

关键词： discrete element method large eddy simulation microparticle particle agglomeration

Wafer‑Scale Vertical 1D GaN Nanorods/2D MoS_(2)/PEDOT:PSS for Piezophototronic Effect‑Enhanced Self‑Powered Flexible Photodetectors

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Nano-Micro Letters 2025年第3期17卷 102-116页

作者： Xin Tang hongsheng Jiang Zhengliang lin Xuan wang Wenliang wang Guoqiang Li State Key Laboratory of Luminescent Materials and Devices South China University of TechnologyGuangzhou 510640People’s Republic of China Department of Electronic Materials School of Materials Science and EngineeringSouth China University of TechnologyGuangzhou 510640People’s Republic of China

van der Waals(vdW)heterostructures constructed by lowdimensional(0D,1D,and 2D)materials are emerging as one of the most appealing systems in next-generation flexible ***,hand-stacked vdW-type photodetectors are not compatible with large-areaarray fabrication and show unimpressive performance in self-powered ***,vertical 1D GaN nanorods arrays(NRAs)/2D MoS_(2)/PEDOT:PSS in wafer scale have been proposed for self-powered flexible photodetectors arrays *** as-integrated device without external bias under weak UV illumination exhibits a competitive responsivity of 1.47 A W^(−1)and a high detectivity of 1.2×10^(11)Jones,as well as a fast response speed of 54/71μs,thanks to the strong light absorption of GaN NRAs and the efficient photogenerated carrier separation in type-II ***,the strain-tunable photodetection performances of device have been ***,the device at−0.78%strain and zero bias reveals a significantly enhanced photoresponse with a responsivity of 2.47 A W^(−1),a detectivity of 2.6×10^(11)Jones,and response times of 40/45μs,which are superior to the state-of-the-art self-powered flexible *** work presents a valuable avenue to prepare tunable vdWs heterostructures for self-powered flexible photodetection,which performs well in flexible sensors.

关键词： Vertical nanorod arrays van der Waals heterostructure Piezophototronic effect Self-powered photodetection Flexible sensors

Simulations of electron zebra stripes in the inner radiation belt using a composite empirical electric field model

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Science China Earth Sciences 2025年第1期68卷 27-38页

作者： Ziyang wang Ying LIU Qiugang ZONG hong ZOU Yuguang YE Xuzhi ZHOU Chao YUE Shan wang Institute of Space Physics and Applied Technology Peking University State Key Laboratory of Lunar and Planetary Science Macau University of Science and Technology

The phenomenon termed “zebra stripes” manifests as regular patterns in the energy-space(L shell) spectrum of energetic electrons(ranging from tens to hundreds ke V) within the inner radiation belt. These structures exhibit drift-periodic behavior and commonly arise from large-scale electric field perturbations near the substorm onsets. In this study, we introduce a composite electric field model and replicate the formation, structure, and evolution of zebra stripes using a bounce-averaged test particle code under this electric field model. High-resolution measurements of energetic electrons obtained from the Van Allen Probes and the recently launched Macao Science Satellites-1 are used as initial conditions and served to validate our test particle simulations. Comparative analyses between observed data and simulations demonstrate our test particle method's efficacy in capturing zebra stripes' general behavior. Moreover, the composite model proves capable of reproducing realistic variations in the electric field within the inner radiation belt to a certain extent. Nevertheless, subtle differences emerge in the flux strength and the positions of stripes. These disparities primarily stem from limitations inherent in the electric field model and the initial conditions of the simulation. Acknowledging that the model represents an average case, it is conceivable that real-world scenarios may deviate from the average, thereby introducing variations in the observed phenomena.

关键词： Zebra stripes Inner radiation belt Large-scale electric fields Van Allen Probes Macao Science Satellite

Pyroptosis,ferroptosis,and autophagy in spinal cord injury:regulatory mechanisms and therapeutic targets

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Neural Regeneration Research 2025年第10期20卷 2787-2806页

作者： Qingcong Zheng Du wang Rongjie lin Weihong Xu Department of Spinal Surgery the First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Arthritis Clinical and Research Center Peking University People’s HospitalBeijingChina Department of Orthopedic Surgery Fujian Medical University Union HospitalFuzhouFujian ProvinceChina

Regulated cell death is a form of cell death that is actively controlled by *** studies have shown that regulated cell death plays a key role after spinal cord *** and ferroptosis are newly discovered types of regulated cell deaths that have been shown to exacerbate inflammation and lead to cell death in damaged spinal ***,a complex form of cell death that is interconnected with various regulated cell death mechanisms,has garnered significant attention in the study of spinal cord *** injury triggers not only cell death but also cellular survival *** signaling pathways play pivotal roles in influencing the processes of both deterioration and repair in spinal cord injury by regulating pyroptosis,ferroptosis,and ***,this review aims to comprehensively examine the mechanisms underlying regulated cell deaths,the signaling pathways that modulate these mechanisms,and the potential therapeutic targets for spinal cord *** analysis suggests that targeting the common regulatory signaling pathways of different regulated cell deaths could be a promising strategy to promote cell survival and enhance the repair of spinal cord ***,a holistic approach that incorporates multiple regulated cell deaths and their regulatory pathways presents a promising multi-target therapeutic strategy for the management of spinal cord injury.

关键词： autophagy cell death ferroptosis inflammation pathological mechanisms pyroptosis regulated cell death regulatory pathways spinal cord injury therapeutic targets

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公路交叉口照明和限速对视觉搜索能力的影响

吉林大学学报（工学版） 2023年第8期53卷 2287-2297页

作者：李洪涛王琳虹李俊达吉林大学交通学院长春130022 中汽研软件测评(天津)有限公司天津300300

为解决夜间车辆在村镇无信号公路交叉口易与自行车或行人碰撞的问题,基于注意分配理论将驾驶人视野图片亮度、凝视点分布面积、注视点一次马尔可夫平稳分布和累计注视时间作为评价指标,结合Logistics回归构建视觉搜索能力量化模型。设... 详细信息

为解决夜间车辆在村镇无信号公路交叉口易与自行车或行人碰撞的问题,基于注意分配理论将驾驶人视野图片亮度、凝视点分布面积、注视点一次马尔可夫平稳分布和累计注视时间作为评价指标,结合Logistics回归构建视觉搜索能力量化模型。设计模拟驾驶试验,采集6种车速和4种照明下的数据验证模型,结果表明模型的正确分类百分比为93.45%。不同照明限速方案的对比结果表明,夜间村镇附近公路交叉口段限速应设为50 km/h,路面平均照度应不低于10 lx。

关键词：交通运输系统工程视觉认知特性注意分配理论环境照明夜间最高限速视觉搜索能力

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Burden of nutritional deficiencies among children and adolescents aged 0 to 19 years in China,1990 to 2015

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Chinese Medical Journal 2021年第24期134卷 3012-3014页

作者： Yuan-Yuan Xu Xin-Ying Zeng Xiu Qiu Li-Wen Fang Zhuo-Qun wang Mai-Geng Zhou lin-hong wang National Center for Chronic and Noncommunicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 100050China National Center for Women and Children’s Health Chinese Center for Disease Control and PreventionBeijing 100081China Guangzhou Women and Children’s Medical Center GuangzhouGuangdong 510623China

To the Editor:Nutritional deficiency is one of the major public health concerns in low-and middle-income *** and adolescents are particularly vulnerable to nutritional deficiencies due to their rapid growth and development *** 2015,nutritional deficiency was one of the top ten global causes of years lived with disability(YLDs)among children and adolescents in 195 countries and territories.[1]Nutritional deficiencies may have irreversible effects on the physical and cognitive development of children and adolescents,which may result in higher morbidity and mortality,and decreased productivity later in *** deficiencies are one of the major health concerns among children and adolescents in *** 2010,the deaths of Chinese children 0 to 14 years of age caused by nutritional deficiencies accounted for about 11.46%of total deaths in Chinese residents due to nutritional deficiencies.[2]Under-standing the disease burden of nutritional deficiencies among children and adolescents in the whole country is helpful for effective disease control and prevention,which may promote improving the health for all in the future.

关键词： income nutritional prevention

Offload Strategy for Edge Computing in Satellite Networks Based on Software Defined Network

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Computers, Materials & Continua 2025年第1期82卷 863-879页

作者： Zhiguo Liu Yuqing Gui lin wang Yingru Jiang Communication and Network Laboratory Dalian UniversityDalian116622China College of Environment and Chemical Engineering Dalian UniversityDalian116622China

Satellite edge computing has garnered significant attention from researchers;however,processing a large volume of tasks within multi-node satellite networks still poses considerable *** sharp increase in user demand for latency-sensitive tasks has inevitably led to offloading bottlenecks and insufficient computational capacity on individual satellite edge servers,making it necessary to implement effective task offloading scheduling to enhance user *** this paper,we propose a priority-based task scheduling strategy based on a Software-Defined Network(SDN)framework for satellite-terrestrial integrated networks,which clarifies the execution order of tasks based on their ***,we apply a Dueling-Double Deep Q-Network(DDQN)algorithm enhanced with prioritized experience replay to derive a computation offloading strategy,improving the experience replay mechanism within the Dueling-DDQN ***,we utilize the Deep Deterministic Policy Gradient(DDPG)algorithm to determine the optimal resource allocation strategy to reduce the processing latency of *** results demonstrate that the proposed d3-DDPG algorithm outperforms other approaches,effectively reducing task processing latency and thus improving user experience and system efficiency.

关键词： Satellite network edge computing task scheduling computing offloading