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Transcription factor GLK1 promotes anthocyanin biosynthesis via an MBW complex-dependent pathway in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2023年第6期65卷 1521-1535页

作者： Yan Li Wei Lei Zuxu Zhou Yanlin Li Dawei Zhang Honghui lin Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment State Key Laboratory of Hydraulics and Mountain River EngineeringCollege of Life ScienceSichuan UniversityChengdu 610064China Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation SWU-TAAHC Medicinal Plant Joint R&D CentreSchool of Life SciencesSouthwest UniversityChongqing 400715China

Anthocyanins are important natural plant pigments and play diverse roles in plant growth and *** function as screens to protect photosynthetic tissues from ***,the regulatory mechanisms underlying the biosynthesis and spatial accumulation pattern of anthocyanins remain some unresolved ***,we demonstrate that the GARP-type transcription factor GOLDEN2-LIKE 1(GLK1)functions as a positive factor in anthocyanin ***1 enhances the transcriptional activation activities of MYB75,MYB90,and MYB113 via direct proteinprotein interactions to increase the expression of anthocyanin-specific biosynthetic *** accumulate in an acropetal manner in *** also found that the expression pattern of GLK1 overall mimicked the accumulation pattern of anthocyanin from the base of the main stem to the shoot *** on these findings,we established a working model for the role of GLK1 in anthocyanin accumulation and propose that GLK1mediates the spatial distribution pattern of anthocyanins by affecting the transcriptional activation activities of MYB75,MYB90,and MYB113.

关键词： anthocyanin biosynthesis GLK1 MYB75 transcriptional activation activity

Bio-inspired supramolecular self-assembly towards soft nanomaterials

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Frontiers of Computer Science 2011年第3期5卷 247-265页

作者： Yiyang lin Chuanbin MAO Department of Chemistry and Biochemistry University of Oklahoma Stephenson Life Sciences Research Center 101 Stephenson Parkway Norman Oklahoma 73019 USA

Supramolecular self-assembly has proven to be a reliable approach towards versatile nanomaterials based on multiple weak intermolecular forces. In this review, the development of bio-inspired supramolecular self-assembly into soft materials and their applications are summarized. Molecular systems used in bio-inspired ＂bottom- up self-assembly＂ involve small organic molecules, peptides or proteins, nucleic acids, and viruses. Self-assembled soft nanomaterials have been exploited in various applications such as inorganic nanomaterial synthesis, drug or gene delivery, tissue engineering, and so on.

关键词： supramolecular self-assembly soft material peptide nucleic acid virus

Human hepatocytes loaded in 3D bioprinting generate mini-liver

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Hepatobiliary & Pancreatic Diseases International 2016年第5期15卷 512-518页

作者： Cheng Zhong Hai-Yang Xie lin Zhou Xiao Xu Shu-Sen Zheng Key Laboratory of Combined Multi-organ Transplantation Ministry of Public HealthFirst Affiliated HospitalZhejiang University School of Medicine

BACKGROUND: Because of an increasing discrepancy be-tween the number of potential liver graft recipients and the number of organs available, scientists are trying to create artiifcial liver to mimic normal liver function and therefore, to support the patient’s liver when in dysfunction. 3D printing technique meets this purpose. The present study was to test the feasibility of 3D hydrogel scaffolds for liver engineering. METHODS: We fabricated 3D hydrogel scaffolds with a bioprinter. The biocompatibility of 3D hydrogel scaffolds was tested. Sixty nude mice were randomly divided into four groups, with 15 mice in each group: control, hydrogel, hydro-gel with L02 (cell line hl-7702), and hydrogel with hepatocyte growth factor (HGF). Cells were cultured and deposited in scaffolds which were subsequently engrafted into livers after partial hepatectomy and radiation-induced liver damage (RILD). The engrafted tissues were examined after two weeks. The levels of alanine aminotransferase (ALT), aspartate amino-transferase (AST), albumin, total bilirubin, CYP1A2, CYP2C9, glutathione S-transferase (a-GST), and UDP-glucuronosyl transferase (UGT-2) were compared among the groups. He-matoxylin-eosin (HE) staining and immunohistochemistry of cKit and cytokeratin 18 (CK18) of engrafted tissues were evalu-ated. The survival time of the mice was also compared among the four groups. RESULTS: 3D hydrogel scaffolds did not impact the viability of cells. The levels of ALT, AST, albumin, total bilirubin, CY-P1A2, CYP2C9, a-GST and UGT-2 were signiifcantly improved in mice engrafted with 3D scaffold loaded with L02 compared with those in control and scaffold only (P CONCLUSION: 3D scaffold has the potential of recreating live

关键词： 3D printing hepatocyte liver tissue engineering

Regulation of shear stress on rolling behaviors of hl-60 cells on P-selectin

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Science China(Physics,Mechanics & Astronomy) 2014年第10期57卷 1998-2006页

作者： linG YingChen FANG Ying YANG XiaoFang LI QuHuan lin QinYong WU JianHua School of Life Sciences Sun Yat-Sen University School of Bioscience and Bioengineering South China University of Technology Institute of Biomechanics South China University of Technology

Circulating leukocytes in trafficking to the inflammatory sites, will be first tether to, and then roll on the vascular surface. This event is mediated through specific interaction of P-selectin and P-selectin glycoprotein ligand-1 （PSGL-1）, and regulated by hemodynamics. Poor data were reported in understanding P-selectin-mediated rolling. With the flow chamber technique, we herein observed hl-60 cell rolling on P-selectin with or without 3% Ficoll at various wall shear stresses from 0.05 to 0.4 dyn/cm：. The results demonstrated that force rather than transport regulated the rolling, similar to rolling on L- and E-selectin. The rolling was accelerated quickly by an increasing force below the optimal shear threshold of 0.15 dyn/cm2 first and then followed by a slowly decelerating phase starting at the optimum, showing a catch-slip transition and serving as a mechanism for the rolling. The catch-slip transition was completely reflected to the tether lifetime and other rolling parameters, such as the mean and fractional stop time. The narrow catch bond regime stabilized the rolling quickly, through steeply increasing frac- tional stop time to a plateau of about 0.85. Data presented here suggest that the low shear stress threshold serves as a mecha- nism for most cell rolling events through P-selectin.

关键词： P-selectin P-selectin glycoprotein ligand-1 cell adhesion shear stress

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hl-2M支撑系统制造工艺研究

核聚变与等离子体物理 2021年第S1期41卷 420-424页

作者：乔涛刘德权林涛郑小舟 hl-2M研制团队核工业西南物理研究院成都610041 武汉重型机床集团有限公司武汉430000

在hl-2M装置支撑组件的研制中,采用了特制控氮不锈钢、结合GMS310焊丝和Ar(96%)+N2(4%)保护气体的焊接工艺以及低速和大进给量的机加方式把磁导率控制在≤1.03的设计范围内。为保障hl-2M装置装配时线圈系统的精确定位和安装,在研制阶段... 详细信息

在hl-2M装置支撑组件的研制中,采用了特制控氮不锈钢、结合GMS310焊丝和Ar(96%)+N2(4%)保护气体的焊接工艺以及低速和大进给量的机加方式把磁导率控制在≤1.03的设计范围内。为保障hl-2M装置装配时线圈系统的精确定位和安装,在研制阶段对极向场线圈支撑子系统进行了预装。

关键词： hl-2M装置支撑部件焊接磁导率预装

Isochamaejasmin induces apoptosis in leukemia cells through inhibiting Bcl-2 family proteins

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Chinese Journal of Natural Medicines 2015年第9期13卷 660-666页

作者： ZHANG Shou-De SHAN Lei LI Wei LI Hong-lin ZHANG Wei-Dong Shanghai Key Laboratory of New Drug Design State Key Laboratory of Bioreactor Engineering School of Pharmacy East China University of Science and Technology School of Pharmacy Second Military Medical University Qinghai Academy of Agriculture and Forestry Science

The biflavonoid isochamaejasmin is mainly distributed in the root of Stellera chamaejasme L.(Thymelaeaceae) that is used in traditional Chinese medicine(TCM) to treat tumors, tuberculosis, and psoriasis. Herein, isochamaejasmin was found to show similar bioactivity against Bcl-2 family proteins to the reference Bcl-2 ligand(–)-gossypol through 3D similarity search. It selectively bound to Bcl-xL and Mcl-1 with Ki values being 1.93 ± 0.13 μmol·L-1 and 9.98 ± 0.21 μmol·L-1, respectively. In addition, isochamaejasmin showed slight growth inhibitory activity against hl-60 with IC50 value being 50.40 ± 1.21 μmol·L-1 and moderate growth inhibitory activity against K562 cells with IC50 value being 24.51 ± 1.62 μmol·L-1. Furthermore, isochamaejasmin induced apoptosis of K562 cells by increasing the intracellular expression levels of proteins of the cleavage of caspase-9, caspase-3, and PARP which involved in the Bcl-2-induced apoptosis pathway. These results indicated that isochamaejasmin induces apoptosis in leukemia cells by inhibiting the activity of Bcl-2 family proteins, providing evidence for further studying the underlying anti-cancer mechanism of S. chamaejasme L..

关键词： Stellera chamaejasme L. Isochamaejasmin Bcl-2 3D Similarity search (–)-Gossypol Apoptosis

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hl-2A等离子体边缘极向剩余胁强的研究

核聚变与等离子体物理 2018年第1期38卷 1-7页

作者：龙婷聂林柯锐许敏吴一帆郭栋王占辉袁博达龚少博刘灏 hl-2A团队核工业西南物理研究院成都610041

首次报导了托卡马克等离子体边缘与湍流相关的极向剩余胁强剖面的测量结果。采用外中平面往复式静电探针阵列对hl-2A托卡马克边缘的极向湍流动量输运进行研究。在没有外部动量注入的欧姆放电下,剩余胁强为有限值、且其空间剖面在等离子... 详细信息

首次报导了托卡马克等离子体边缘与湍流相关的极向剩余胁强剖面的测量结果。采用外中平面往复式静电探针阵列对hl-2A托卡马克边缘的极向湍流动量输运进行研究。在没有外部动量注入的欧姆放电下,剩余胁强为有限值、且其空间剖面在等离子体边缘具有明显的径向梯度,表明托卡马克等离子体边缘存在极向动量源。由动量源产生的动量主要以扩散形式向与剩余胁强相反的方向传播,最终的结果是等离子体边缘存在有限的雷诺胁强。在最后闭合磁面以内0.5~2cm区域,剩余胁强的梯度提供自发旋转的力矩,由该力矩引起的动量产生与由速度梯度引起的动量扩散共同导致了雷诺胁强出现负梯度,造成动量沉积,从而驱动极向平衡流。

关键词：湍流极向动量输运极向旋转剩余胁强静电探针托卡马克等离子体

β-Carboline alkaloids from the roots of Peganum harmala L.

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Chinese Journal of Natural Medicines 2024年第2期22卷 171-177页

作者： LI Shengge ZHANG Qin WANG Yuetong lin Bin LI Dahong HUA Huiming HU Xu Henan Key Laboratory of Zhang Zhongjing Formulate and Herbs for Immunoregulation Zhang Zhongjing Traditional School of Chinese MedicineNanyang Institute of TechnologyNanyang 473004China Key Laboratory of Structure-Based Drug Design&Discovery Ministry of EducationShenyang Pharmaceutical UniversityShenyang 110016China Wuya College of Innovation Shenyang Pharmaceutical UniversityShenyang 110016China School of Pharmaceutical Engineering Shenyang Pharmaceutical UniversityShenyang 110016China

This study reports the isolation of four newβ-carboline alkaloids(1−4)and six previously identified alkaloids(5−10)from the roots of Peganum harmala *** these compounds,1 and 2 were characterized as rareβ-carboline-quinazoline dimers exhibiting axial *** 3 possessed a unique 6/5/6/7 tetracyclic ring system with an azepine ring,and compound 4 was a novel annomontineβ-*** structures of these compounds were elucidated by spectroscopic data and quantum mechanical *** biosynthetic pathways of 1−3 were ***,the cytotoxicity of some isolates against four cancer cell lines(hl-60,A549,MDA-MB-231,and DU145)was ***,compound 4 exhibited significant cytotoxicity against hl-60,A549,and DU145 cells with IC_(50) values of 12.39,12.80,and 30.65μmol·L^(−1),***,compound 2 demonstrated selective cytotoxicity against hl-60 cells with an IC_(50) value of 17.32μmol·L^(−1).

关键词： β-Carboline alkaloids Peganum harmala Structure elucidation Cytotoxic activity

Quantitative Evaluation of the Compatibility Effects of Aidi Injection on the Treatment of Hepatocellular Carcinoma Using Targeted Metabolomics:A New Strategy on the Mechanism Study of an Anticancer Compound in Traditional Chinese Medicine

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World Journal of Traditional Chinese Medicine 2021年第1期7卷 111-119页

作者： Ran Liu lin-lin Zhu Chun-Yu Yu Ya-Ping Shuai ling-ling Sun Kai-Shun Bi Qing Li School of Pharmacy Shenyang Pharmaceutical UniversityShenyang 110016China Liaoning Institute of Drug Inspection and Testing Shenyang 110030China

Objective:Compound traditional Chinese medicine(CTCM)with the application of compatibility from multiple active ingredients with multiple-specific targets can achieve a synergistic effect on cancer *** study is aimed to observe the compatibility effects of Aidi injection on the treatment of hepatocellular carcinoma and to explore the mechanism of ***:Aidi injection is a clinical compound prescription containing Mylabris,Ginseng,Astragalus,and Acanthopanax,which can inhibit tumor growth and induce *** this study,the anticancer activity of Aidi injection,as well as its disassembled and combined compositions,had been evaluated by varying levels of polyamine biomarkers on human hepatoma Hep-G2 cells detected using ultrahigh-performance liquid chromatography-tandem mass ***:According to the different variations in polyamine levels,it was revealed that Mylabris and Ginseng had an antitumor effect,while Astragalus acted as an assistant and Acanthopanax had weak anticancer *** increased level of polyamines in Hep-G2 cells had been found in hl-7702 *** combining Mylabris and Ginseng,polyamine levels went close to the normal level,which was even more marked when Astragalus was *** injection acted like the combination of Mylabris,Ginseng,and ***:This study established a quantitative evaluation of the compatibility effects of Aidi injection based on polyamine biomarkers and evaluated the consistency of its anticancer effect,providing a manner to research the efficacy evaluation of ***,the correlation between polyamine metabolism and anticancer activity can be used in anticancer drug screening.

关键词： Compatibility effects evaluation compound traditional Chinese medicine hepatocellular carcinoma polyamine biomarker