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Cell-cycle dependent nuclear gene delivery enhances the effects of E-cadherin against tumor invasion and metastasis

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Signal Transduction and Targeted Therapy 2023年第6期8卷 3039-3054页

作者： Liting Xie Jieqiong Wang liming song Tianan Jiang Fei Yan Department of Ultrasound The First Affiliated HospitalZhejiang University School of MedicineHangzhou310058China CAS Key Laboratory of Quantitative Engineering Biology Shenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China Department of Orthopedics Zhujiang HospitalSouthern Medical UniversityGuangzhou510280China

Gene delivery is the process by which foreign DNA is transferred to host cells,released from intracellular vesicles,and transported to the nuclei for transcription.This process is frequently inefficient and difficult to control spatiotemporally.We developed a gene delivery strategy that uses ultrasound to directly deliver plasmid DNA into nuclei via gas vesicles(GVs)-based intracellular cavitation.pDNA-binding GVs can be taken up by cells and cause intracellular cavitation when exposed to acoustic irradiation and delivering their pDNA payloads into nuclei.Importantly,GVs can remain stable in the cytoplasm in the absence of acoustic irradiation,allowing for temporally controlled nuclear gene delivery.We were able to achieve spatiotemporal control of E-cadherin nuclear gene delivery in this manner,demonstrating its efficacy in tumor invasion and metastasis inhibition.Interestingly,we discovered that nuclear gene delivery of E-cadherin during the G2/M phase of the cell cycle in C6 tumor cells inhibited tumor invasion and metastasis more effectively than during the G1 and S phases.The gene delivery of E-cadherin at the G2/M phase resulted in significantly lower expression of Fam50a,which reduced Fam50a/Runx2 interaction and led to reduced transactivation of MMP13,an important factor for epithelial-mesenchymal transition,as observed in a molecular mechanism assay.Thus,using remote acoustic control of intracellular cavitation of pDNA-GVs,we developed a high spatiotemporally controllable gene delivery strategy and achieved stronger tumor invasion and metastasis inhibition effects by delivering the E-cadherin gene at the G2/M phase.

关键词： invasion metastasis inhibited

Study on direct point spread function measurement of capillary plate collimators with divergent X-ray beams

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Radiation Detection Technology and Methods 2023年第3期7卷 321-336页

作者： Tianming Li Tao Luo Yupeng Xu Huilin He liming song Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of ScienceBeijing 100049China University of Chinese Academy of Sciences Chinese Academy of SciencesBeijing 100049China

Purpose The Filed of View(FOV)of eXTP/LAD is limited by lead-glass capillary plate collimators placed in front of the Silicon Drift Detectors(SDDs)for reducing the background contamination caused by the photons of Cosmic X-ray Background(CXB)leaking from outside the FOV.The core quality parameters of lead-glass capillary plate collimators are Open Area Ratio(OAR)and FOV.The performance of lead-glass capillary plate collimators is determined by its design specification such as pore-to-pore misalignment,pore perpendicularity with collimator surface and pore diameter.Since the design specification is the result of a large number of micropores,we use the full width at half maximum(FWHM)and peak value of point spread function(PSF)to characterize the capillary plate collimator performance.In order to quickly evaluate the performances of collimators,we developed a direct PSF measurement method using divergent beams.Methods In this paper,the simulation package of the divergent beamsmeasurement setup is developed based on Geant4.The simulation parameters of scintillator absorption length are corrected by experimental data.We simulate the PSF of collimators with different distribution of pore diameter and misalignment by parallel beams and divergent beams.By comparing the peak value and FWHM of collimators PSF,the feasibility in replacing parallel beams measurement with divergent beams measurement is studied.Results It is verified that the influence of the geometric size of 33μm radius of the micro-focus of X-ray tube can be ignored.The results show that the FWHM of collimators is increased with error root mean square error(RMS)of the distribution of collimators pore diameter and pore inclination angle.The peak value of PSF is mainly influenced by pore non-parallelism of the pores,and it decreases with an increase in pore inclination angle.Comparison of parallel beams PSF and divergent beams PSF shows well consistency of peak value and FWHM of collimator.But,the peak value of the divergent beams PSF is mainly affected by t

关键词： Geant4 EXTP/LAD Capillary plate collimators Divergent X-ray beams measurment

The design and implementation of GECAM satellite payload performancemonitoring software

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Radiation Detection Technology and Methods 2022年第1期6卷 3-11页

作者： Peng Zhang Xiang Ma Yue Huang Shaolin Xiong Shijie Zheng liming song Ge Ou Yanqi Du Jing Liang Hong Wu Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China University of Chinese Academy of Science Beijing 100049China School of Computing and Artificial Intelligence Southwest Jiaotong UniversityChengdu 611756China

Background The Gravitational wave high-energy Electromagnetic Counterpart All-sky Monitor(GECAM)is primarily designed to spot gamma-ray bursts corresponding to gravitational waves.In order to achieve stable observations from various astronomical phenomena,the payload performance need to be monitored during the in-orbit operation.Method This article describes the design and implementation of GECAM satellite payload performance monitoring(GPPM)software.The software extracts the payload status and telescope observations(light curves,energy spectrums,characteristic peak fitting of energy spectrums,etc)from the payload data.Considering the large amount of payload status parameters in the engineering data,we have designed a method of parameter processing based on the configuration tables.This method can deal with the frequent changes of the data formats and facilitate program maintenance.Payload status and performance are monitored through defined thresholds and monitoring reports.The entire software is implemented in python language and the huge amount of observation data is stored in MongoDB.Conclusion The design and implementation of GPPM software have been completed,tested with ground and in-orbit payload data.The software can monitor the performance of GECAM payload effectively.The overall design of the software and the data processing method can be applied to other satellites.

关键词： GECAM Payload Data process Python

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基于CT扫描数据的黄鳍金枪鱼鱼体三维重构

水产学报 2024年第3期48卷 128-134页

作者：宋利明李金霖张敏上海海洋大学海洋科学学院上海海洋大学国家远洋渔业工程技术研究中心

为研究鱼类分类与其内部结构，实验利用X射线计算机断层(CT)扫描技术对黄鳍金枪鱼鱼体进行扫描，通过医学影像软件Mimics (MIS＿Research＿21.0)对CT扫描数据进行处理，对黄鳍金枪鱼的骨骼、鳔和肌肉进行三维重构并测定了相应部分的体... 详细信息

为研究鱼类分类与其内部结构，实验利用X射线计算机断层(CT)扫描技术对黄鳍金枪鱼鱼体进行扫描，通过医学影像软件Mimics (MIS＿Research＿21.0)对CT扫描数据进行处理，对黄鳍金枪鱼的骨骼、鳔和肌肉进行三维重构并测定了相应部分的体积和表面积。结果显示：(1) CT扫描可获得清晰的黄鳍金枪鱼骨骼、肌肉和鳔的影像；(2)医学影像软件Mimics可计算出黄鳍金枪鱼的骨骼、鳔和肌肉的体积和表面积，其体积分别占鱼体体积的7.76%、0.88%和91.36%，表面积分别占鱼体表面积的29.52%、0.74%和69.74%。研究表明：(1)使用X射线CT扫描技术是目前对鱼类损伤较小、鱼体骨骼、鳔和肌肉成像最便捷、最直观的方法；(2)医学影像软件Mimics可用于鱼体三维重构、计算鱼类骨骼、鳔和肌肉的体积和表面积；(3) CT扫描可用于鱼类学、鱼病学和鱼类声学回波强度的深入研究。实验可为鱼类学、鱼病学和鱼类声学回波强度等的深入研究提供参考。

关键词：黄鳍金枪鱼鱼体 CT扫描三维重构 Mimics软件

基于动态规划的原油管道运行能耗优化技术研究

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石油石化节能与计量 2024年第6期14卷 61-65页

作者：宋丽明大庆油田有限责任公司第七采油厂

随着管道老化和站内设备劣化,原油管道整体能耗呈逐年上升趋势。在考虑泵状态、加热炉状态、变频器状态、泵转速、加热炉功率、节流阀压降等决策变量的基础上,建立了以总运行费用最小为目标函数的原油管道运行能耗优化模型,结合总传热... 详细信息

随着管道老化和站内设备劣化,原油管道整体能耗呈逐年上升趋势。在考虑泵状态、加热炉状态、变频器状态、泵转速、加热炉功率、节流阀压降等决策变量的基础上,建立了以总运行费用最小为目标函数的原油管道运行能耗优化模型,结合总传热系数和水力摩阻系数修正值,采用动态规划算法对最优运行方案进行求解。结果表明,参数修正后,进站温度的平均相对误差为3.41%,进站压力的平均相对误差为3.60%,满足后续优化需求;水力优化结果考虑了沿程站场电价的影响,站内节流阀均保持常开,说明站间没有剩余压力浪费;热力优化结果保证了进站温度均高于原油凝点2~5℃,没有剩余进站温度浪费;总运行费用较优化前降低了12.4%,经济效益明显。研究结果可为原油管道节能运行方案的制定和实施提供理论依据。

关键词：动态规划原油管道能耗优化总传热系数水力摩阻系数

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新能源汽车电池包防火涂料的现状与进展

涂料工业 2024年第3期54卷 66-71,85页

作者：刘文庆朱罗毅宋立明 PPG全球涂料创新研究院天津300457

新能源汽车电池包防火涂料属于涂料应用新领域,市场前景广阔,目前已有多家涂料厂家推出电池包防火涂料,以双组分超薄膨胀型防火涂料为主。因其契合电池包轻量化、灵活设计及自动化的需求,防火涂料开始在电池包中应用,但对应用场景、施... 详细信息

新能源汽车电池包防火涂料属于涂料应用新领域,市场前景广阔,目前已有多家涂料厂家推出电池包防火涂料,以双组分超薄膨胀型防火涂料为主。因其契合电池包轻量化、灵活设计及自动化的需求,防火涂料开始在电池包中应用,但对应用场景、施工和失效特点等方面的针对性有所欠缺,又一定程度上限制了其大规模应用。针对新能源电池包热失控时高温火焰、颗粒冲击等特点,综述了新型电池包防火涂料研究进展,如通过添加防火树脂提高耐火性能、陶瓷化提高炭层的强度、加入防火以外的新功能等,为新能源电池包防火涂料的开发提供了新的视野。

关键词：新能源汽车动力电池包热失控防火涂料膨胀型

采用样条曲面非轴对称端壁成型的高压涡轮动叶气动优化

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西安交通大学学报 2024年第3期58卷 38-48页

作者：王杰王政欧阳玉清陶志宋立明李军西安交通大学能源与动力工程学院西安710049 中国航发湖南动力机械研究所湖南株洲412002

为了提升高负荷涡轮级的气动效率,发展了基于样条曲面的非轴对称端壁造型方法。以该参数化造型方法为基础,结合高效智能优化算法和经过校核的数值仿真方法,建立了涡轮非轴对称端壁设计优化平台,并以某小展弦比高压涡轮级为研究对象,以... 详细信息

为了提升高负荷涡轮级的气动效率,发展了基于样条曲面的非轴对称端壁造型方法。以该参数化造型方法为基础,结合高效智能优化算法和经过校核的数值仿真方法,建立了涡轮非轴对称端壁设计优化平台,并以某小展弦比高压涡轮级为研究对象,以效率为优化目标,以流量为约束条件,在级环境和发动机工况下开展了非轴对称端壁优化设计。结果表明:优化设计后的涡轮动叶相对于参考设计,涡轮级的总总效率提升0.26%;非轴对称端壁造型改变了动叶下端壁附近的压力分布,动叶吸力面侧压力系数相对于参考设计显著提升,这降低了动叶叶片通道内的横向压力梯度,抑制了通道中的二次流动;非轴对称端壁造型改变了叶片通道中的涡系结构,相对于参考设计,非轴对称端壁造型使得马蹄涡压力面分支在叶片通道内部沿着叶片压力面迁移,在靠近通道出口的位置才汇入通道涡,这削弱了通道涡的强度,进而降低了气动损失,提高了涡轮级效率。

关键词：高压涡轮非轴对称端壁气动性能优化设计

X-Ray Fine Structure of a Limb Solar Flare Revealed by Insight-HXMT,RHESSI and Fermi

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Research in Astronomy and Astrophysics 2022年第5期22卷 47-55页

作者： Ping Zhang Wei Wang Yang Su Shuangnan Zhang liming song Fangjun Lu Shu Zhang Department of Physics and Technology Wuhan UniversityWuhan 430072China WHU-NAOC Joint Center for Astronomy Wuhan UniversityWuhan 430072China Purple Mountain Observatory of the Chinese Academy of Sciences Nanjing 210023China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China

We conduct a detailed analysis of an M1.3 limb flare occurring on 2017 July 3,which have the X-ray observations recorded by multiple hard X-ray telescopes,including Hard X-ray Modulation Telescope(Insight-HXMT),Ramaty High Energy Solar Spectroscopic Imager(RHESSI),and the Fermi Gamma-ray Space Telescope(Fermi).Joint analysis has also used the extreme ultraviolet(EUV)imaging data from the Atmospheric Imaging Assembly(AIA)aboard the Solar Dynamic Observatory.The hard X-ray spectral and imaging evolution suggest a lower corona source,and the non-thermal broken power law distribution has a rather low break energy~15 keV.The EUV imaging shows a rather stable plasma configuration before the hard X-ray peak phase,and accompanied by a filament eruption during the hard X-ray flare peak phase.Hard X-ray image reconstruction from RHESSI data only shows one foot point source.We also determined the DEM for the peak phase by SDO/AIA data.The integrated EM beyond 10 MK at foot point onset after the peak phase,while the>10 MK source around reconnection site began to fade.The evolution of EM and hard X-ray source supports lower corona plasma heating after non-thermal energy dissipation.The combination of hard X-ray spectra and images during the limb flare provides the understanding on the interchange of non-thermal and thermal energies,and relation between lower corona heating and the upper corona instability.

关键词： Sun flares-Sun corona-Sun X-rays-gamma-rays-plasmas-radiation mechanisms non-thermal-radiation mechanisms thermal

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高龄二胎孕妇心理一致感的影响因素分析

临床医学研究与实践 2024年第7期9卷 53-56页

作者：宋黎明开封市妇幼保健院产一科河南开封475002

目的调查分析高龄二胎孕妇的心理一致感评分及其相关影响因素。方法收集2017年10月至2021年5月我院妇产科收治的842例高龄二胎孕妇的临床资料,通过一般资料问卷、心理一致感量表(SCO-13)进行调查,分析高龄二胎孕妇心理一致感的影响因素... 详细信息

目的调查分析高龄二胎孕妇的心理一致感评分及其相关影响因素。方法收集2017年10月至2021年5月我院妇产科收治的842例高龄二胎孕妇的临床资料,通过一般资料问卷、心理一致感量表(SCO-13)进行调查,分析高龄二胎孕妇心理一致感的影响因素。结果842例不同孕周高龄二胎孕妇的SCO-13评分为(58.22±6.18)分。单因素分析发现,不同SCO-13评分高龄二胎孕妇的年龄、体质量、妊娠期并发症、对胎儿性别期待、计划妊娠、孕周比较,差异具有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,年龄≥40岁、体质量≥60 kg、妊娠期并发症、对胎儿性别期待、孕早期为影响高龄二胎孕妇SCO-13评分的危险因素,而有妊娠计划为影响高龄二胎孕妇SCO-13评分的保护因素(P<0.05)。结论高龄二胎孕妇心理一致感处于较低水平,年龄、体质量、妊娠期并发症、对胎儿性别期待、孕周、妊娠计划均为其影响因素。为提高心理一致感水平,临床可根据相关影响因素对高龄二胎孕妇进行针对性干预。

关键词：高龄二胎孕妇心理一致感影响因素