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Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimethoate degraders

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Frontiers of Environmental Science & Engineering 2024年第1期18卷 59-69页

作者： Luning Lian Yi Xing Dayi Zhang Longfei jiang Mengke Song Bo jiang School of Energy and Environmental Engineering University of Science&Technology BeijingBeijing 100083China Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants University of Science&Technology BeijingBeijing 100083China National Environmental and Energy Science and Technology International Cooperation Base University of Science&Technology BeijingBeijing 100083China College of New Energy and Environment Jilin UniversityChangchun 130021China Guangzhou Institute of Geochemistry Chinese Academy of SciencesGuangzhou 510640China College of Natural Resources and Environment South China Agricultural UniversityGuangzhou 510642China National Engineering Laboratory for Site Remediation Technologies Beijing 100015China

Microorganisms are crucial in the bioremediation of organophosphorus pesticides. However, most functional microorganisms (> 99%) are yet to be cultivated. This study applied two cultivation-independent approaches, DNA-SIP and magnetic-nanoparticle mediated isolation (MMI), to identify the functional microorganisms in degrading dimethoate in agricultural soils. MMI identified five dimethoate degraders: Pseudomonas, Bacillus, Ramlibacter, Arthrobacter, and Rhodococcus, whereas DNA-SIP identified three dimethoate degraders: Ramlibacter, Arthrobacter, and Rhodococcus. Also, MMI showed higher resolution than DNA-SIP in identifying functional microorganisms. Two organic phosphohydrolase (OPH) genes: ophC2 and ophB, were involved in dimethoate metabolism, as revealed by DNA-SIP and MMI. The degradation products of dimethoate include omethoate, O,O,S-trimethyl thiophosphorothioate, N-methyl-2-sulfanylacetamide, O,O-diethyl S-hydrogen phosphorodithioate, O,O,O-trimethyl thiophosphate, O,O,S-trimethyl thiophosphorodithioate, and O,O,O-trimethyl phosphoric. This study emphasizes the feasibility of using SIP and MMI to explore the functional dimethoate degraders, expanding our knowledge of microbial resources with cultivation-independent approaches.

关键词： Stable isotope probing(SIP) Magnetic-nanoparticle mediated isolation(MMI) Dimethoate Biodegradation Cultivation-independent approach

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林樊家油田精细油藏描述及开发技术浅析

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中外能源 2009年第1期14卷 64-67页

作者：姜利民中国石化胜利油田分公司滨南采油厂山东滨州256606

利用精细地层对比及微构造描述技术、沉积微相划分及储层评价技术、综合物探技术对东营凹陷林樊家油田进行了油藏精细描述。结果表明林樊家油田馆陶组油藏埋藏浅,油层胶结疏松,地层砂粒度中值小,出砂严重。因此应用了绕丝筛管砾石充填... 详细信息

利用精细地层对比及微构造描述技术、沉积微相划分及储层评价技术、综合物探技术对东营凹陷林樊家油田进行了油藏精细描述。结果表明林樊家油田馆陶组油藏埋藏浅,油层胶结疏松,地层砂粒度中值小,出砂严重。因此应用了绕丝筛管砾石充填防砂技术和压裂防砂技术进行治理。两口井的效果分别为:平均单井日产液19.9t/d,日产油4.6t/d,年累计产油2641t;平均单井日产液19.4t/d,日产油8.3t/d,年累计产油4704t。

关键词：沉积微相精细描述防砂技术

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Three-channel CMOS transimpedance amplifier for LiDAR sensor receiver

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Journal of Systems Engineering and Electronics 2024年第1期35卷 74-80页

作者： LIU Ruqing ZHU Jingguo jiang Yan LI Feng jiang Chenghao MENG Zhe Photoelectric R&D Center Academy of Opto-ElectronicsChinese Academy of SciencesBeijing 100094China

For time-of-flight(TOF)light detection and ranging(LiDAR),a three-channel high-performance transimpedance amplifier(TIA)with high immunity to input load capacitance is presented.A regulated cascade(RGC)as the input stage is at the core of the complementary metal oxide semiconductor(CMOS)circuit chip,giving it more immunity to input photodiode detectors.A simple smart output interface acting as a feedback structure,which is rarely found in other designs,reduces the chip size and power consumption *** circuit is designed using a 0.5μm CMOS process technology to achieve low *** device delivers a 33.87 dB?transimpedance gain at 350 *** a higher input load capacitance,it shows a-3 dB bandwidth of 461 MHz,indicating a better detector tolerance at the front end of the *** a 3.3 V supply voltage,the device consumes 5.2 mW,and the total chip area with three channels is 402.8×597.0μm2(including the test pads).

关键词： transimpedance amplifier(TIA) three-channel regulated cascade(RGC) light detection and ranging(LiDAR)

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Operational requirements analysis method based on question answering of WEKG

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Journal of Systems Engineering and Electronics 2024年第2期35卷 386-395页

作者： ZHANG Zhiwei DOU Yajie XU Xiangqian MA Yufeng jiang jiang TAN Yuejin College of Systems Engineering National University of Defense TechnologyChangsha 410073China

The weapon and equipment operational requirement analysis(WEORA) is a necessary condition to win a future war,among which the acquisition of knowledge about weapons and equipment is a great challenge. The main challenge is that the existing weapons and equipment data fails to carry out structured knowledge representation, and knowledge navigation based on natural language cannot efficiently support the WEORA. To solve above problem, this research proposes a method based on question answering(QA) of weapons and equipment knowledge graph(WEKG) to construct and navigate the knowledge related to weapons and equipment in the WEORA. This method firstly constructs the WEKG, and builds a neutral network-based QA system over the WEKG by means of semantic parsing for knowledge navigation. Finally, the method is evaluated and a chatbot on the QA system is developed for the WEORA. Our proposed method has good performance in the accuracy and efficiency of searching target knowledge, and can well assist the WEORA.

关键词： operational requirement analysis weapons and equipment knowledge graph(WEKG) question answering(QA) neutral network

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测量雷达的距离多普勒耦合修正方法研究

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电子测试 2018年第3期29卷 72-73,71页

作者：蒋立民 91245部队

线性调频脉冲压缩体制去对雷达进行测量,在对于运动目标给予精准探测的过程中,因为其受到了距离-多普勒耦合效应带来的影响,多普勒频移会令雷达出现测距上的误差,并且这一误差和目标自身的径向速度之间是一种正比的关系。对于出现的这... 详细信息

线性调频脉冲压缩体制去对雷达进行测量,在对于运动目标给予精准探测的过程中,因为其受到了距离-多普勒耦合效应带来的影响,多普勒频移会令雷达出现测距上的误差,并且这一误差和目标自身的径向速度之间是一种正比的关系。对于出现的这种问题,本文在对多普勒频移对于线性调频脉压产生影响进行分析的基础上,得出了距离多普勒耦合准确的修正方式,同时使用雷达去对数据实测给予了有效的验证,其自身很好的对于测量雷达自身的测距精度给予了保证。

关键词：线性调频脉冲压缩距离多普勒耦合测距误差

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Gut microbial interactions based on network construction and bacterial pairwise cultivation

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Science China(Life Sciences) 2024年第8期67卷 1751-1762页

作者： Min-Zhi jiang Chang Liu Chang Xu He jiang Yulin Wang Shuang-jiang Liu State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266000China State Key Laboratory of Microbial Resources and Environmental Microbiology Research Center(EMRC)Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Association networks are widely applied for the prediction of bacterial interactions in studies of human gut ***,the experimental validation of the predicted interactions is challenging due to the complexity of gut microbiomes and the limited number of cultivated *** this study,we addressed this challenge by integrating in vitro time series network(TSN)associations and cocultivation of TSN taxon *** samples were collected and used for cultivation and enrichment of gut microbiome on YCFA agar plates for 13 *** cells were harvested for DNA extraction and metagenomic sequencing.A total of 198 metagenome-assembled genomes(MAGs)were *** dynamics of bacteria growing on the YCFA agar were used to infer microbial association *** experimentally validate the interactions of taxon pairs in networks,we selected 24 and 19 bacterial strains from this study and from the previously established human gut microbial biobank,respectively,for pairwise *** co-culture experiments revealed that most of the interactions between taxa in networks were identified as neutralism(51.67%),followed by commensalism(21.67%),amensalism(18.33%),competition(5%)and exploitation(3.33%).Genome-centric analysis further revealed that the commensal gut bacteria(helpers and beneficiaries)might interact with each other via the exchanges of amino acids with high biosynthetic costs,short-chain fatty acids,and/or *** also validated 12 beneficiaries by adding 16 additives into the basic YCFA medium and found that the growth of 66.7%of these strains was significantly *** approach provides new insights into the gut microbiome complexity and microbial interactions in association *** work highlights that the positive relationships in gut microbial communities tend to be overestimated,and that amino acids,short-chain fatty acids,and vitamins are contributed to the positive relationships.

关键词： human gut microbiome gut microbial pairwise interaction co-culture association network microbial community

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Hybrid Block Diagonalization Precoding for Multi-User Weighted Sum-Rate Maximization

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China Communications 2024年第8期21卷 127-141页

作者： Su Xiaofeng jiang Yi Key Laboratory for Information Science of Electromagnetic Waves(MoE) Department of Communication Science and EngineeringFudan UniversityShanghai 200433China

This paper studies large-scale multi-input multi-output(MIMO)orthogonal frequency division multiplexing(OFDM)communications in a broadband frequency-selective channel,where a massive MIMO base station(BS)communicates with multiple users equipped with *** develop a hybrid precoding design to maximize the weighted sum-rate(WSR)of the users by optimizing the digital and the analog precoders *** the digital part,we employ block-diagonalization to eliminate inter-user interference and apply water-filling power allocation to maximize the *** the analog part,the optimization of the PSN is formulated as an unconstrained problem,which can be efficiently solved by a gradient descent *** results show that the proposed block-diagonal hybrid precoding algorithm can outperform the existing works.

关键词： block-diagonal gradient descent hybrid precoding iterative optimization MIMO-OFDM multi-user water-filling power allocation weighted sum-rate maximization

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信用管理——农资企业面临的新课题

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农资科技 2002年第2期 5-7页

作者：姜利民中国农资集团公司

自农资经营逐步市场化后,信用问题和拖欠风险问题始终是困扰农资企业发展的一大顽症。大量的逾期应收帐款和呆帐、坏帐不仅使企业的流动资金紧张,经营亏损(或潜亏),而且导致恶性循环,农资企业间的信用关系遭到严重破坏,诚信度急剧下降... 详细信息

自农资经营逐步市场化后,信用问题和拖欠风险问题始终是困扰农资企业发展的一大顽症。大量的逾期应收帐款和呆帐、坏帐不仅使企业的流动资金紧张,经营亏损(或潜亏),而且导致恶性循环,农资企业间的信用关系遭到严重破坏,诚信度急剧下降。信用管理已经成为农资企业必须面对和研究的新课题。

关键词：信用管理农资企业信用风险风险控制

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单脉冲雷达数据采集及处理方法

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电子测试 2017年第10期28卷 77-78,64页

作者：蒋立民 91245部队辽宁葫芦岛125001

在载人航天、编目空间目标以及对战区导弹进行预防警报等方面单脉冲测量雷达有着极为重要的实用价值,而脉冲雷达的核心就是数据的采集与处理,数据采集与处理在脉冲雷达完成各项工作时发挥的作用是十分重要的。本文结合了进行研究的单脉... 详细信息

在载人航天、编目空间目标以及对战区导弹进行预防警报等方面单脉冲测量雷达有着极为重要的实用价值,而脉冲雷达的核心就是数据的采集与处理,数据采集与处理在脉冲雷达完成各项工作时发挥的作用是十分重要的。本文结合了进行研究的单脉冲测量雷达,对脉冲雷达关于数据处理方面的功能、结构组成、工作原理以及工作中使用的算法和实现的方法进行了系统的阐述。针对新型单脉冲测量雷达的一些关键特点进行分析。

关键词：单脉冲测量雷达 RCS 雷达标校测量精度

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Weighted multilinear p-adic Hardy operators and commutators on p-adic Herz-type spaces

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Applied Mathematics(A Journal of Chinese Universities) 2024年第3期39卷 554-570页

作者： MA Teng ZHOU jiang College of Mathematics and System Sciences Xinjiang UniversityUrumqi 830046China

In this paper,we introduce the weighted multilinear p-adic Hardy operator and weighted multilinear p-adic Ces`aro operator,we also obtain the boundedness of these two operators on the product of p-adic Herz spaces and p-adic Morrey-Herz spaces,the corresponding operator norms are also established in each ***,the boundedness of commutators of these two operators with symbols in central bounded mean oscillation spaces and Lipschitz spaces on p-adic Morrey-Herz spaces are also given.

关键词： Morrey-Herz spaces weighted multilinear p-adic Hardy operator commutators operator norms

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