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Enhancing single-cell encapsulation in droplet microfluidics with fine-tunable on-chip sample enrichment

Microsystems & Nanoengineering 2024年第1期10卷 149-160页

作者： Tao Tang Hao Zhao Shaofei Shen like yang Chwee Teck Lim Department of Biomedical Engineering National University of Singapore117583 SingaporeSingapore Integrative Sciences and Engineering Programme NUS Graduate SchoolNational University of Singapore119077 SingaporeSingapore Shanxi Key Lab for Modernization of TCVM College of Life ScienceShanxi Agricultural UniversityTaiguShanxi 030801China Institute for Health Innovation&Technology National University of Singapore117599 SingaporeSingapore Mechanobiology Institute National University of Singapore117411 SingaporeSingapore Institute for Digital Molecular Analytics and Science Nanyang Technological University636921 SingaporeSingapore

Single-cell encapsulation in droplet microfluidics is commonly hindered by the tradeoff between cell suspension density and on-chip focusing *** this study,we introduce a novel droplet microfluidic chip to overcome this *** chip comprises a double spiral focusing unit,a flow resistance-based sample enrichment module with fine-tunable outlets,and a crossflow droplet generation *** a low-density cell/bead suspension(2×10^(6) objects/mL),cells/beads are focused into a near-equidistant linear arrangement within the double spiral *** excess water phase is diverted while cells/beads remain focused and sequentially encapsulated in individual *** performance was assessed through numerical simulations and experiments at three flow rates(40,60,80μL/min),demonstrating successful focusing at 40 and 80μL/min for beads and cells,*** addition,both simulation and experimental results revealed that the flow resistance at the sample enrichment module is adjustable by punching different outlets,allowing over 50%of the aqueous phase to be ***8n-based droplet detection algorithms realized the counting of cells/beads in droplets,statistically demonstrating single-cell and bead encapsulation rates of 72.2%and 79.2%,*** the results indicate that this on-chip sample enrichment approach can be further developed and employed as a critical component in single-cell encapsulation in water-in-oil droplets.

关键词： beads outlet sample

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Research on Converting Cultivated Land into Forests and Regional Sustainable Development

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Ecological Economy 2005年第2期1卷 100-105页

作者： ChangJinbao ShaRula ZhangQiuliang yanglike CollegeofForest InnerMongoliaAgricultureUniversityHuhehot010019P.R.China

The capability of sustainable development of Wuchuan County is evaluated comprehensively with theories and methods of regional sustainable development capability evaluation, which are extensively applied at home and abroad. The results indicate that the developing speed and patterns of Wuchuan County cannot satisfy the demand of the regional sustainable development. Moreover, the patterns, distributions, tree species, growing patterns, and other aspects must consider the restriction of ecological water utilization. It is put forward that the standards for selecting the converting lands are slope, desertification degree, and *** addition, the control of soil erosion and the prevention of land desertification are selected as the dualpurpose of the converting project.

关键词： Converting Cultivated Land into Forests Regional sustainable development Evaluation.

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Improving flight performance of UAVs by ice shape modulation

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Chinese Journal of Aeronautics 2024年第8期37卷 49-62页

作者： Jiajun ZHANG Xuecheng LIU Hua LIANG like XIE Biao WEI Haohua ZONG Yun WU Yinghong Li National Key Lab of Aerospace Power System and Plasma Technology Air Force Engineering UniversityXi’an 710038China Institute of Aero-Engine School of Mechanical EngineeringXi’an Jiaotong UniversityXi’an 710049China

Aircraft icing poses a great threat to flight *** response to the characteristics of high-power consumption,large volume,and heavy weight of traditional anti-/de-icing technologies,the concept of ice shape modulation is proposed,which is called ice tolerant ***,the flight performance of Unmanned Aerial Vehicle(UAV)was compared in three states:no ice,full ice,and modulated ice through flight *** was found that ice shape modulation has a significant improvement effect on the aerodynamic performance of aircraft under icing *** the three modulated ice shape conditions in this experiment,the lift coefficient of the UAV under different ice shape modulation conditions increased by 18%–33%,and the stalling angle was delayed by 3°-5°.Subsequently,the pressure distribution,streamlines in the flow field,and detached vortex distribution of the UAV model in these three states were obtained through numerical simulation,to study the mechanism of ice shape modulation on the aerodynamic performance of *** simulation found that the reason for the improvement of the wings effect after ice shape modulation is that the modulated area forms a leading-edge protrusion structure similar to a vortex *** structure prolongs the mixed flow region on the wings surface and reduces the trend of flow separation,which plays a role in increasing lift and reducing drag for UAVs under icing ***,a reverse reachable set that can be used for unexpected state recovery is used as the definition of flight safety boundaries,and an aircraft dynamics model is established to obtain flight safety boundaries for different *** has found that the flight safety boundary of the UAV in a no ice state is greater than that in a modulated ice state,and the safety boundary in a modulated ice state is greater than that in a full ice *** with the full ice state,the flight safety boundary after modulation has expanded by 27.0%.The sch

关键词： Ice shape modulation Aerodynamic performance Reachable set Flight safety UAV

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Ice-covered Environmental Adaptability Assessment for Overhead Contact System in High-speed Railways

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Chinese Journal of Electrical Engineering 2024年第2期10卷 70-79页

作者： Huan Zhang Wenfu Wei like Pan Zefeng yang Guizao Huang Yuxin Liu Xing Chen Ziqian yang Guangning Wu School of Electrical Engineering Southwest Jiaotong UniversityChengdu 611756China National Rail Transit Electrification and Automation Engineering Technology Research Center Southwest Jiaotong UniversityChengdu 611756China China Academy of Railway Sciences Corporation Limited Beijing 100000China

High-speed trains rely on pantograph-catenary systems(PCSs)to collect electrical energy from power ***,the dynamic interaction between the pantograph and the catenary system may become mismatched once ice accumulates on the overhead *** frequent arcing may occur within the PCS during train operation,posing an unpredictable threat to operational ***,it is crucial to evaluate the ability of overhead contact system(OCS)to withstand ice-covered variability during line desgin.A new strategy is proposed to evaluate the adaptive performance of an OCS under various icing ***,a dynamic model considering icing conditions is constructed to simulate the interaction within the *** different OCS structures with various icing thicknesses are *** parameters of the contact force within the PCS and proportion of high-possibility arcing moments are *** dependence of the contact force on the icing thickness and pantograph displacement has been illustrated in the form of cloud ***,the OCS sensitivity coefficient is calculated,and ice-covered environmental adaptability assessments for the five different OCS structures are compared.

关键词： Overhead contact system environmental adaptability sensitivity coefficient icing thickness contact force

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Fresh-Keeping Decision and Coordination of Fresh Agricultural Product Supply Chain Considering Carbon Cap-and-Trade under Different Dominance

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Journal of Systems Science and Systems Engineering 2024年第1期33卷 30-51页

作者： yang yang Guanxin Yao China Institute for Agricultural Equipment Industrial Development Jiangsu UniversityZhenjiang212013China Jiangsu Modern Logistics Research Base Yangzhou UniversityYangzhou225009China

In the context of low carbon,this paper discussed the impact of the carbon cap-and-trade policy on the fresh-keeping decision-making of two-echelon fresh agricultural product supply chains under different dominance,and designed cost-sharing contracts to coordinate the supply chain of fresh agricultural products dominated by suppliers and retailers *** results showed that:dominance has no effect on the fresh-keeping decision and total revenue of fresh agricultural product supply chain,but it affects the internal income distribution,and dominance does not always bring more benefits;the implementation of carbon cap-and-trade reduces the fresh-keeping decision-making of fresh agricultural products supply chain and reduces the free-rider income of followers;the role of higher carbon trading price is twofold,which not only brings about the speculation of leading enterprises,but also promotes the application of low-carbon technologies;consumers’high preference for freshness,low-cost and high-efficiency low-carbon technology are all conducive to improving the fresh-keeping efforts and benefits of the supply chain;cost-sharing contracts can coordinate the supply chain of fresh agricultural products.

关键词： Dominant power carbon cap-and-trade policy fresh agricultural product supply chain fresh-keeping decision-making

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Overview of radar detection methods for low altitude targets in marine environments

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Journal of Systems Engineering and Electronics 2024年第1期35卷 1-13页

作者： yang Yong yang Boyu School of Electronic Science National University of Defense TechnologyChangsha 410073China

In this paper,a comprehensive overview of radar detection methods for low-altitude targets in maritime environments is presented,focusing on the challenges posed by sea clutter and multipath *** performance of the radar detection methods under sea clutter,multipath,and combined conditions is categorized and summarized,and future research directions are outlined to enhance radar detection performance for low-altitude targets in maritime environments.

关键词： radar sea clutter multipath scattering detection low altitude target

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我国西部能源型城市发展模式的系统动力学分析--以鄂尔多斯市为例

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内蒙古科技与经济 2022年第6期 3-8,21页

作者：杨立科徐燕鄂尔多斯市规划编制研究中心鄂尔多斯市社保中心内蒙古鄂尔多斯017000

文章运用系统动力学方法,构建鄂尔多斯城市发展系统结构模型,进行模拟分析。探求鄂尔多斯城市发展的最优模式,找出适合能源型城市发展的共同模式。研究结果表明:城市发展是多个系统相互作用的结果;鄂尔多斯的城市发展与生态建设最终稳... 详细信息

文章运用系统动力学方法,构建鄂尔多斯城市发展系统结构模型,进行模拟分析。探求鄂尔多斯城市发展的最优模式,找出适合能源型城市发展的共同模式。研究结果表明:城市发展是多个系统相互作用的结果;鄂尔多斯的城市发展与生态建设最终稳定在共生共存之中;控制城市污染、加快生态建设、稳定经济建设、提升居民生活水平是鄂尔多斯健康发展的最佳途径。

关键词：城市发展生态建设系统动力学

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Selective nano-buckling improves the performance of graphene logic transistors

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National Science Review 2024年第2期11卷 95-96页

作者： Tong yang Ming yang Department of Applied Physics The Hong Kong Polytechnic University Research Centre for Nanoscience and Nanotechnology The Hong Kong Polytechnic University

The massive integration of silicon-based transistors into modern electronic devices greatly increases power consumption and heat generation/dissipation[1]. In this concern, transistors based on atomically-thin graphene that exhibit high electric and thermoelectric conductivities with negligible contact resistance,

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Design Optimization and Operating Performance of S-CO_(2) Brayton Cycle under Fluctuating Ambient Temperature and Diverse Power Demand Scenarios

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Journal of Thermal Science 2024年第1期33卷 190-206页

作者： yang Jingze yang Zhen DUAN Yuanyuan Key Laboratory for Thermal Science and Power Engineering of MOE Beijing Key Laboratory for CO_(2) Utilization and Reduction TechnologyTsinghua UniversityBeijing 100084China

The supercritical CO_(2)(S-CO_(2)) Brayton cycle is expected to replace steam cycle in the application of solar power tower system due to the attractive potential to improve efficiency and reduce *** the concentrated solar power plant with thermal energy storage is usually located in drought area and used to provide a dispatchable power output,the S-CO_(2) Brayton cycle has to operate under fluctuating ambient temperature and diverse power demand *** addition,the cycle design condition will directly affect the off-design *** this work,the combined effects of design condition,and distributions of ambient temperature and power demand on the cycle operating performance are analyzed,and the off-design performance maps are proposed for the first time.A cycle design method with feedback mechanism of operating performance under varied ambient temperature and power demand is introduced *** show that the low design value of compressor inlet temperature is not conductive to efficient operation under low loads and sufficient output under high ambient *** average yearly efficiency is most affected by the average power demand,while the load cover factor is significantly influenced by the average ambient *** multi-objective optimization,the optimal solution of designed compressor inlet temperature is close to the minimum value of35℃ in Delingha with low ambient temperature,while reaches 44.15℃ in Daggett under the scenario of high ambient temperature,low average power demand,long duration and large value of peak load during the peak temperature *** the cycle designed with compressor inlet temperature of 35℃ instead of 44.15℃ in Daggett under light industry power demand,the reduction of load cover factor will reach 0.027,but the average yearly efficiency can barely be improved.

关键词： supercritical CO_(2)Brayton cycle ambient temperature fluctuating power demand scenarios design optimization off-design performance

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A physics-informed deep learning framework for spacecraft pursuit-evasion task assessment

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Chinese Journal of Aeronautics 2024年第5期37卷 363-376页

作者： Fuyunxiang yang Leping yang Yanwei ZHU College of Aerospace Science and Engineering National University of Defense TechnologyChangsha 410073China

Qualitative spacecraft pursuit-evasion problem which focuses on feasibility is rarely studied because of high-dimensional dynamics,intractable terminal constraints and heavy computational *** this paper,A physics-informed framework is proposed for the problem,providing an intuitive method for spacecraft threat relationship determination,situation assessment,mission feasibility analysis and orbital game rules *** the first time,situation adjustment suggestions can be provided for the weak player in orbital ***,a dimension-reduction dynamics is derived in the line-of-sight rotation coordinate system and the qualitative model is determined,reducing complexity and avoiding the difficulty of target set presentation caused by individual ***,the Backwards Reachable Set(BRS)of the target set is used for state space partition and capture zone ***-time analysis can eliminate the influence of changeable initial state and enable the proposed framework to analyze plural situations ***,a time-dependent Hamilton-Jacobi-Isaacs(HJI)Partial Differential Equation(PDE)is established to describe BRS evolution driven by dimension-reduction dynamics,based on level set ***,Physics-Informed Neural Networks(PINNs)are extended to HJI PDE final value problem,supporting orbital game rules summarization through capture zone evolution ***,numerical results demonstrate the feasibility and efficiency of the proposed framework.

关键词： Spacecraft pursuit-evasion Qualitative differential game Physics-Informed Neural Networks(PINNs) Reachability analysis Hamilton-Jacobi-Isaacs(HJI) Partial Differential Equations(PDEs)

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