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The action mechanism by which C1q/tumor necrosis factor-related protein-6 alleviates cerebral ischemia/reperfusion injury in diabetic mice

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Neural Regeneration Research 2024年第9期19卷 2019-2026页

作者： Bo Zhao Mei Li Bingyu Li Yanan Li Qianni Shen Jiabao Hou Yang Wu lijuan gu Wenwei Gao Department of Anesthesiology Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina Central Laboratory Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina Department of Critical Care Medicine Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina

Studies have shown that C1q/tumor necrosis factor-related protein-6 (CTRP6) can alleviate renal ischemia/reperfusion injury in mice. However, its role in the brain remains poorly understood. To investigate the role of CTRP6 in cerebral ischemia/reperfusion injury associated with diabetes mellitus, a diabetes mellitus mouse model of cerebral ischemia/reperfusion injury was established by occlusion of the middle cerebral artery. To overexpress CTRP6 in the brain, an adeno-associated virus carrying CTRP6 was injected into the lateral ventricle. The result was that oxygen injury and inflammation in brain tissue were clearly attenuated, and the number of neurons was greatly reduced. In vitro experiments showed that CTRP6 knockout exacerbated oxidative damage, inflammatory reaction, and apoptosis in cerebral cortical neurons in high glucose hypoxia-simulated diabetic cerebral ischemia/reperfusion injury. CTRP6 overexpression enhanced the sirtuin-1 signaling pathway in diabetic brains after ischemia/reperfusion injury. To investigate the mechanism underlying these effects, we examined mice with depletion of brain tissue-specific sirtuin-1. CTRP6-like protection was achieved by activating the sirtuin-1 signaling pathway. Taken together, these results indicate that CTRP6 likely attenuates cerebral ischemia/reperfusion injury through activation of the sirtuin-1 signaling pathway.

关键词： brain C1q/tumor necrosis factor-related protein-6 cerebral apoptosis diabetes inflammation ischemia/reperfusion injury neuron neuroprotection oxidative damage Sirt1

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儿童急性皮肤衰竭研究进展

中华烧伤与创面修复杂志 2024年第4期40卷 395-400页

作者：尹希常菲陈杰顾丽娟葛燚红毛佳敏苏州大学附属张家港医院烧伤整形科苏州215600 苏州大学附属张家港医院神经内科苏州215600 苏州大学附属张家港医院泌尿外科苏州215600 苏州大学附属张家港医院普外科苏州215600 苏州大学附属张家港医院产科苏州215600

急性皮肤衰竭(ASF)是指发生在皮肤和皮下组织因血流动力学不稳定和/或低灌注所导致的不可避免的损伤。目前国内外对于成人ASF已有一定的认识和报道,但对儿童ASF鲜有报道。该文从儿童ASF的定义、病理生理学变化、危险因素、临床表现与管... 详细信息

急性皮肤衰竭(ASF)是指发生在皮肤和皮下组织因血流动力学不稳定和/或低灌注所导致的不可避免的损伤。目前国内外对于成人ASF已有一定的认识和报道,但对儿童ASF鲜有报道。该文从儿童ASF的定义、病理生理学变化、危险因素、临床表现与管理等方面进行综述,提出建议以期为儿童ASF的临床诊断和治疗提供思路,推动对儿童ASF的深入研究。

关键词：儿童压力性溃疡多器官功能衰竭皮肤急性皮肤衰竭

过度执教:我国青少年足球教学中一个普遍的隐性问题

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中国体育教练员 2024年第1期32卷 36-40页

作者：钱惠顾丽娟刘博上海市普陀区青少年业余足球学校上海200333 上海体育大学上海200438

过度执教是制约我国青少年足球发展和足球后备人才培养的一个隐性因素。基于文献资料分析,结合实践考察,对过度执教的内涵、判断、危害及致因进行深层解析。认为:教练员缺乏正确的执教理念、执教经验不足、功利主义作祟、外界环境影响... 详细信息

过度执教是制约我国青少年足球发展和足球后备人才培养的一个隐性因素。基于文献资料分析,结合实践考察,对过度执教的内涵、判断、危害及致因进行深层解析。认为:教练员缺乏正确的执教理念、执教经验不足、功利主义作祟、外界环境影响等是造成过度执教的原因。过度执教的危害在于:阻断球员思维过程,破坏动作技能的学习和掌握,扼杀球员的创造性。为防止过度执教,教练员应积极为球员创设学习情境、正确选择执教理念、相信球员、摒弃功利主义、选择合理执教策略等。

关键词：校园足球过度执教执教理念执教环境执教策略

miR-126对皮肤黑色素瘤C8161迁移、侵袭作用机制的研究

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激光生物学报 2024年第2期33卷 167-175页

作者：禚欣欣顾丽娟周晓晗淮安市第五人民医院皮肤性病美容科淮安223300

本研究主要观察微小RNA-126-3p(miR-126)的差异性表达对人皮肤黑色素瘤(CM)细胞株C8161的迁移、侵袭性的影响,并探究了Janus蛋白酪氨酸激酶2/信号传导与转录激活因子3(JAK2/STAT3)通路和上皮-间质转化(EMT)过程在其中的角色。采用细胞... 详细信息

本研究主要观察微小RNA-126-3p(miR-126)的差异性表达对人皮肤黑色素瘤(CM)细胞株C8161的迁移、侵袭性的影响,并探究了Janus蛋白酪氨酸激酶2/信号传导与转录激活因子3(JAK2/STAT3)通路和上皮-间质转化(EMT)过程在其中的角色。采用细胞转染法实现miR-126的差异性表达,用JAK2/STAT3通路抑制剂AG490、激动剂Coumermycin A1处理miR-126差异表达的细胞,并将C8161细胞分为对照组(不做转染,不做药物处理)、阴性对照(NC)组(转染模拟物对照,不做药物处理)、miR-126组(转染miR-126模拟物,不做药物处理)、miR-126+通路抑制剂组(转染miR-126模拟物后AG490处理)和miR-126+通路激动剂组(转染miR-126模拟物后Coumermycin A1处理)。采用实时荧光定量PCR(RT-qPCR)检测miR-126的表达水平;利用蛋白免疫印迹法测定EMT关键蛋白和JAK2/STAT3通路蛋白的表达水平;利用细胞计数试剂盒-8(CCK-8)、划痕愈合试验、Transwell小室联合基质胶法分别检测细胞的活力、迁移和侵袭能力。与NC组相比,miR-126模拟物转染使miR-126的表达水平在miR-126组显著提高(^(#)P<0.05),与此同时,相对细胞活力、细胞迁移率和细胞侵袭数均显著降低(^(#)P<0.05)。此外,miR-126组的上皮型钙黏蛋白(E-cadherin)高于NC组(^(#)P<0.05),而波形蛋白(vimentin)、神经钙黏蛋白(N-cadherin)、纤维连接蛋白(FN)、JAK2、STAT3、p-JAK2、p-STAT3蛋白的表达水平和p-JAK2/JAK2、p-STAT3/STAT3的比值均低于NC组(^(#)P<0.05)。更重要的是,与NC组和miR-126组相比,JAK2和STAT3蛋白的表达水平的上述指标在miR-126+通路抑制剂组中的变化更显著(^(#)P<0.05和^(&)P<0.05),而上述指标在miR-126+通路激活剂组中的变化趋势减弱(^(#)P<0.05和^(&)P<0.05),其中细胞侵袭数和FN、JAK2、STAT3蛋白的表达水平在miR-126+通路激动剂组和NC组之间无显著差异。过表达miR-126抑制人CM细胞的活力、迁移和侵袭能力,而该作用很可能是通过阻碍EMT进程、抑制JAK2/STAT3通路的活化来实现的。这些研究结果有助于为人CM的临床治疗提供新的理论依据,为其治疗方法提供新的策略。

关键词：皮肤黑色素瘤微小RNA-126-3p Janus蛋白酪氨酸激酶2/信号传导与转录激活因子3 迁移侵袭

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Semi-clathrate hydrate based carbon dioxide capture and separation techniques

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Frontiers of Environmental Science & Engineering 2023年第12期17卷 1-29页

作者： lijuan gu Hailong Lu Beijing International Center for Gas Hydrate School of Earth and Space SciencesPeking UniversityBeijing 100871China National Engineering Research Center for Gas Hydrate Exploration and Development Guangzhou 511466China

CO_(2)is considered as the main contributor to global warming,and hydrate enclathration is an efficient way for carbon capture and separation(CCS).Semi-clathrate hydrate(SCH)is a type of clathrate hydrate capable of encaging CO_(2)molecules under mild temperature and pressure *** has numerous unique advantages,including high thermal stability,selective absorption of gas molecules with proper size and recyclable,making it a promising candidate for *** SCH based CCS technology is in the developing stage and great efforts have to be conducted to improve the performance that is determined by their thermodynamical and structural *** review summarizes and compares the thermodynamic and structural properties of SCH and quaternary salt hydrates with gas mixtures to be captured and *** on the description of the physical properties of SCH and hydrate of quaternary salts with gas mixture,the CO_(2)capture and separation from fuel gas,flue gas and biogas with SCH are *** review focuses on the use of tetra-nbutyl ammonium halide and tetra-n-butyl phosphonium halide,which are the current application *** review aims to provide guidance for the future applications of SCH.

关键词： Semi-clathrate hydrate Tetra-n-butyl ammonium halide Tetra-n-butyl phosphonium halide Structure Thermodynamical properties CO_(2) capture and separation

“三茶”统筹赋能县域茶产业创新发展策略--以湖州市吴兴区为例

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中国茶叶加工 2024年第1期 46-50页

作者：顾丽娟钱伟红陆鸿英柳丽萍湖州陆羽茶文化研究会浙江湖州313000 湖州市农业科学研究院浙江湖州313000 湖州市吴兴区农业技术推广服务中心浙江湖州313000

“三茶”统筹发展理论引领了新时期茶产业高质量发展的正确方向,指明了发展目标。浙江省湖州市吴兴区作为陆羽《茶经》诞生地,茶文化底蕴深厚,近年来茶产业发展取得一定成效,但总体发展水平不高,发展潜力还未激发。文章通过把握吴兴区... 详细信息

“三茶”统筹发展理论引领了新时期茶产业高质量发展的正确方向,指明了发展目标。浙江省湖州市吴兴区作为陆羽《茶经》诞生地,茶文化底蕴深厚,近年来茶产业发展取得一定成效,但总体发展水平不高,发展潜力还未激发。文章通过把握吴兴区茶产业发展现状,测度了茶产业发展水平,分析了茶文化比较优势及茶旅融合发展现实基础,探讨了吴兴区茶产业创新发展的策略与实现路径。

关键词：茶文化茶产业茶科技吴兴区

Numerical Study of Gas Production from a Methane Hydrate Reservoir Using Depressurization with Multi-wells

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Acta Geologica Sinica(English Edition) 2021年第3期95卷 928-936页

作者： SHANG Shilong gu lijuan LU Hailong College of Engineering Peking UniversityBeijing 100871China School of Earth and Space Sciences Peking UniversityBeijing 100871China Beijing International Center for Gas Hydrate Peking UniversityBeijing 100871China

With the implementation of the production tests in permafrost and offshore regions in Canada,US,Japan,and China,the study of natural gas hydrate has progressed into the stage of technology development for industrial *** depressurization method is considered as a better strategy to produce gas from hydrate reservoirs based on production tests and laboratory ***-well production is proposed to improve gas production efficiency,to meet the requirement for industrial *** evaluating the applicability of multi-well production to hydrate exploitation,a 2D model is established,with numerical simulations of the performance of the multi-well pattern carried *** understand the dissociation behavior of gas hydrate,the pressure and temperature distributions in the hydrate reservoir are specified,and the change in permeability of reservoir sediments is *** results obtained indicate that multi-well production can improve the well connectivity,accelerate hydrate dissociation,enhance gas production rate and reduce water production as compared with single-well production.

关键词： oil and gas upstream unconventional resources natural gas hydrate numerical simulation depressurization

Small-Molecule Fluorophores for Near-Infrared IIb Imaging and Imageguided Therapy of Vascular Diseases

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CCS Chemistry 2022年第12期4卷 3735-3750页

作者： Yang Li Hua Zhu Xiaobo Wang Yan Cui lijuan gu Xiaowen Hou Mengting guan Junzhu Wu Yuling Xiao Xiaoxing Xiong Xianli Meng Xuechuan Hong Department of Neurosurgery Renmin Hospital of Wuhan UniversityState Key Laboratory of VirologyWuhan University School of Pharmaceutical SciencesWuhan 430060 State Key Laboratory of Southwestern Chinese Medicine Resources Innovative Institute of Chinese Medicine and PharmacyChengdu University of Traditional Chinese MedicineChengdu 611137 College of Science Research Center for EcologyLaboratory of Extreme Environmental Biological Resources and Adaptive EvolutionInnovation Center for Traditional Tibetan Medicine Modernization and Quality ControlMedical CollegeTibet UniversityLhasa 850000 Center for Experimental Basic Medical Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery(MOE)Hubei Provincial Key Laboratory of Developmentally Originated DiseaseHubei Province Engineering and Technology Research Center for Fluorinated PharmaceuticalsWuhan 430071 Shenzhen Institute of Wuhan University Shenzhen 518057

Accurate and dynamic visualization of vascular diseases can contribute to restraining further deterioration from diseases in a timely ***,visualization is still unable to precisely determine whether and to what extent blood vessels or brain tissues are ***,we report novel benzobis(1,2,5-thiadiazole)-based second near-infrared region(NIR-II)fluorophores HY1-HY4 with highly twisted structures(55°at the S_(0) state),extremely strong aggregation-induced emission(AIE)characteristics(I/I_(0)>13),and remarkably high fluorescence quantum yields(QYs)(up to 14.45%)in the NIR-II region(>1000 nm)and∼0.27%in the nearinfrared IIb window(NIR-IIb,>1500 nm)in aqueous *** NIR-IIb AIE HY4 dots,high-resolution NIR-IIb fluorescence imaging of revascularization and thrombolysis,and real-time feedback of the therapeutic efficacy of Chinese medicine Dengzhan Xixin injection(DXI)on ischemic stroke,were achieved for the first *** addition,results showed that DXI conferred neuroprotection against cerebral ischemia injury mediated via the angiogenesis *** attractive results provide a new perspective for designing ultrabright NIR-IIb probes for vascular-related phenomena,disease assessment,and precise intraoperative imageguided therapy with a deeper tissue penetration depth and higher resolution.

关键词： aggregation-induced emission NIR-IIb imaging(1500-1700 nm) vascular diseases ischemic stroke image-guided therapy

CD4 T cell deficiency attenuates ischemic stroke, inhibits oxidative stress, and enhances Akt/mTOR survival signaling pathways in mice

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Chinese Neurosurgical Journal 2018年第4期4卷 189-195页

作者： Hongfei Zhang Xiaoxing Xiong lijuan gu Weiying Xie Heng Zhao Department of Neurosurgery Stanford University School of Medicine1201 Welch Rd.MSLS Bldg.Room P306StanfordCA 94305USA Department of Anesthesiology Zhujiang Hospital of Southern Medical UniversityGuangzhouGuangdongChina Department of Neurosurgery Renmin Hospital of Wuhan UniversityWuhanHubeiChina Central Laboratory Renmin Hospital of Wuhan UniversityWuhanHubeiChina Department of Anesthesiology Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouChina

Background: Inhibition of CD4 T cells reduces stroke-induced infarction by inhibiting neuroinflammation in the ischemic brain in experimental stroke. Nevertheless, little is known about its effects on neuronal survival signaling pathways. In this study, we investigated the effects of CD4 T cell deficits on oxidative stress and on the Akt/mTOR cell signaling pathways after ischemic stroke in mice. Methods: MHC II gene knockout C57/BL6 mice, with significantly decreased CD4 T cells, were used. Stroke was induced by 60-min middle cerebral artery (MCA) occlusion. Ischemic brain tissues were harvested for Western blotting. Results: The impairment of CD4 T cell production resulted in smaller infarction. The Western blot results showed that iNOS protein levels robustly increased at 5 h and 24 h and then returned toward baseline at 48 h in wild-type mice after stroke, and gene KO inhibited iNOS at 5 h and 24 h. In contrast, the anti-inflammatory marker, arginase I, was found increased after stroke in WT mice, which was further enhanced in the KO mice. In addition, stroke resulted in increased phosphorylated PTEN, Akt, PRAS40, P70S6, and S6 protein levels in WT mice, which were further enhanced in the animals whose CD4 T cells were impaired. Conclusion: The impairment of CD4 T cell products prevents ischemic brain injury, inhibits inflammatory signals, and enhances the Akt/mTOR cell survival signaling pathways.

关键词： Stroke Neuroinflammation CD4 T cells Akt MTOR PTEN