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安徽省自然资源调查监测数据综合评价指标体系构建研究

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测绘与空间地理信息 2024年第9期47卷 28-32页

作者：孔丽马卫春曲丽佳安徽省基础测绘信息中心安徽合肥230031

针对安徽省耕地、林地、草地、湿地、水等自然资源特性,紧紧围绕守住安全底线、提高利用效率、保护生态功能、改善生活品质、降低环境影响、提升治理水平等6个主题,基于“压力—状态—影响—响应”的业务逻辑,选择41个指标构建了一套清... 详细信息

针对安徽省耕地、林地、草地、湿地、水等自然资源特性,紧紧围绕守住安全底线、提高利用效率、保护生态功能、改善生活品质、降低环境影响、提升治理水平等6个主题,基于“压力—状态—影响—响应”的业务逻辑,选择41个指标构建了一套清晰明确的综合评价指标体系,并选取六安市为试点区域进行验证。评价结果与六安市各县区实际生态经济发展相对状况相符,对开展省级自然资源调查监测数据的评价工作具有参考价值。

关键词：自然资源 PSIR模型综合评价指标体系

Molecular evolution of the exon 2 of CHS genes and the possibility of its application to plant phylogenetic analysis

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Chinese Science Bulletin 2000年第19期45卷 1735-1742页

作者： WANG Jinling qu lijia CHEN Jun GU Hongya CHEN Zhangliang The National Laboratory of Protein Engineering and Plant Genetic Engineering Peking UniversityBeijing 100871China De Montfort University The GatewayLeicesterLEI 9BHUK

The exon 2 of chalcone synthase (CHS) gene is relatively conserved during evolution. In this study, three exon 2 fragments from two species in gymnosperm (Cycas panzhihuaensis, Ginkgo biloba ) and seven from four species in angiosperm (Magnolia denudata, Salix babylonica, Nymphaea tetragona, Camellia japonica) have been amplified by PCR from genomic DNA and sequenced. Together with other 73 sequences of CHS collected from EMBL database and literature, these sequences, which embrace 19 families of gymnosperm and angiosperm, have been analyzed for their phylogenetic relations by parsimony method. The result indicated that sequences from the same systematic family usually grouped together except those from Theaceae, Magnoliaceae and Nymphaeaceae. The relative rate test revealed the rate heterogeneity of CHS genes among the families. For the nucleotide substitution the sequences from Asteraceae and Solanaceae evolve faster than those from the other families analyzed while the sequences from Poaceae,

关键词： chalcone synthase (CHS) PCR phylogeny molecular evolution.

自然资源“三全”调查监测技术体系研究与实践

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测绘与空间地理信息 2024年第2期47卷 76-80页

作者：陈春晖王迎春张伟曲丽佳安徽省基础测绘信息中心安徽合肥230031 安徽省第一测绘院安徽合肥230031

为统筹整合遥感数据源,统一实施地类变化信息提取,优化各项调查监测和业务管理的业务流,探索建立“全范围地类监测、全流程变化跟踪、全业务数据支撑”的“三全”调查监测体系。整合“天空地网”多源协同数据获取、变化监测要素智能提... 详细信息

为统筹整合遥感数据源,统一实施地类变化信息提取,优化各项调查监测和业务管理的业务流,探索建立“全范围地类监测、全流程变化跟踪、全业务数据支撑”的“三全”调查监测体系。整合“天空地网”多源协同数据获取、变化监测要素智能提取、三维时空场景建模与管理、自然资源监测智能化服务平台等关键技术,形成标准统一、手段智能、业务联通、先进实用的自然资源统一调查监测技术体系,更有力地支撑生态文明建设和自然资源管理。

关键词：自然资源调查监测数据获取智能提取三维时空场景

Variation at the transcriptional level among Chinese natural populations of Arabidopsis thaliana in response to cold stress

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Chinese Science Bulletin 2008年第19期53卷 2989-2999页

作者： HE Fei KANG JiuQing ZHOU Xin SU Zhen qu lijia GU HongYa National Laboratory of Protein Engineering and Plant Genetic Engineering Peking-Yale Joint Center for Plant Molecular Genetics and AgroBiotechnology College of Life Sciences Peking University Beijing 100871 China College of Biological Sciences China Agriculture University Beijing 100094 China National Plant Gene Research Center (Beijing) Beijing 100101 China

The Arabidopsis 25K GeneChip (ATH1, Affymetrix) was used to make a survey of the variation of the transcriptional profiles among 5 Chinese natural populations of Arabidopsis thaliana under cold treatment. In normal growth condition, the expression level of 2.26% (513 genes in the population from Jiujiang, Jiangxi, JXjjx) to 6.52% (1482 genes in the population from Tongliang, Chongqing, CQtlx) genes was 2-fold higher than that of Col ecotype. Under cold treatment, the expression of 12.84% (2920 genes in the population from Chenggu, Shaanxi, SXcgx) to 19.46% (4426 genes in the population from Qinghe, Xinjiang, XJqhx) genes was up- or down-regulated by at least two-fold that of their controls. In general, most of up-regulated genes might be the genes essential for plant surviving at low temperature, such as genes in CBF pathway and the genes responsible for synthesizing molecules accumulated for cold tolerance. However, each natural population had some specific genes induced under cold treatment. The data indicated that some of the cold-responding genes were differentiated among the populations distributed in the natural habitats with different climate conditions. CBF3, one of the key tran-scription factor genes in cold responding pathway, showed significant differences in expression among populations. The sequence analysis indicated that the changes in its regulation region caused the dramatic difference in the expression pattern. Further studies on the correlation of the function of the differentially expressed genes and the cold tolerance in different populations may provide some new insight into the molecular mechanism of adaptation to local environment in Arabidopsis thaliana in China.

关键词：中国人口调查基因转录实验研究

Cloning and expression of a cDNA encoding ribosomal protein S4 from Rice (Oryza sativa)

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Chinese Science Bulletin 2000年第2期45卷 168-173页

作者： qu lijia LI Donghui ZHANG Yi LIU Meihua GU Hongya CHEN Zhangliang National Laboratory of Protein Engineering and Plant Genetic Engineering Peking UniversityBeijing 100871China De Montfort University The GatewayLeicester LE1 9BHUK

A cDNA clone, pS4, has been isolated from a cDNA library prepared from rice anthers of about 1.0 mm in length. DNA sequence analysis and database search show that the cDNA encodes a protein which is highly homologous to eukaryotic 80S ribosomal protein subunit 4 (S4). Northern hybridization indicates that this gene expresses in all tissues analyzed although the expression level varies and it cannot be induced by mechanical wounding in leaves. Southern blot analysis demonstrates that this rice S4 gene is from a multigene

关键词： ribosomal protein S4 cDNA clone monocotyledonous plant tissue specificity(rice) 1) The nucleotide sequence data reported is in the EMBL Nucleotide Sequence Database under the accession number Y15009.

Structural and expressional analysis of a cDNA that expresses predominantly in rice stamens

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Chinese Science Bulletin 1998年第9期43卷 765-769页

作者： Yi Zhang lijia qu Meihua Liu Xiaoli Qin Hongya Gu Zhangliang Chen Peking Univ Key Lab Prot Engn & Plant Genet Engn Beijing 100871 Peoples R China

The development of stamen is an important stage during the sexual reproduction in higher plants. Studying the genes that predominantly or even specifically expressed in stamens will aid in understanding the molecular mechanism of stamen development. The structure of a rice CHS-like gene (D5) which predominantly expressed in anthers has been analysized, showing high structural similarity with two other anther-specific genes (A1 and BA42) from Brassica napus. Northern analysis also reveals that the three genes share a similar expression pattern and that the expression of D5 may relate to the early development of pollens. It is concluded that D5, A1 and BA42 represent a group of diverged CHS-like genes which are related to stamen development.

关键词： pollen development meiosis stage rice anther cDNA chalcone synthase.

Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their expression in transgenic rice

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Chinese Science Bulletin 2000年第3期45卷 242-246页

作者： LU Huafei MING Xiaotian qu lijia LIU Meihua LI Jing GU Hongya CHEN Zhangliang State Key Laboratory of Protein Engineering and Plant Genetic Engineering Peking UniversityBeijing 100871China Demontfort University The GatewaysLeicester LEI 9BHUK

The promoter fragments of wheat GstA1 and potato Gst1 have been amplified by PCR, cloned and fused respectively to the minimal promoter sequence of rice actin gene (Act1)) and its 5’ untranslated leader sequence together with GUS. The constructs with 2 chimeric promoters (WGA and PGA) have been transferred into rice in order to analyze their inducibility patterns in transgenic rice plants. The results show that: WGA and PGA are both inducible by elicitors of Pyricularia oryzae in transgenic rice cells; the intron I of rice Act1 gene is important for the heterogenic expression of monocot and dicot promoter elements in rice; and the Act1 minimal promoter and its 5’untranslated leader sequence produced low level background expression in rice.

关键词： pathogen-inducible chimeric promoter gene expression and regulation transgenic rice rice fungal blast disease.

Obtaining transgenic rice resistant to rice fungal blast disease by controlled cell death strategy

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Chinese Science Bulletin 2003年第16期48卷 1753-1759页

作者： MAO Shengji, GU Hongya, qu lijia & CHEN Zhangliang National Key Laboratory of Protein Engineering and Plant Genetic En-gineering, College of Life Sciences, Peking University, Beijing 100871, China Correspondence should be addressed to qu lijia (e-mail: qulj@plum. ***) National Key Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing China

The strategy of the two-component system, composed of Barnase and Barstar which encode RNase and a specific inhibitor to the RNase respectively, is adopted to obtain transgenic rice resistant to rice fungal blast disease. In this study, two chimeric promoters, induced by rice blast fungus pathogen (Magnaporthe grisea), are fused with Barnase respectively to construct two plant expression vec-tors, pWBNBS and pPBNBS together with the Barstar driven by CaMV 35S promoter. The resistance of the transgenic rice lines to rice blast fungus disease and rice blight disease are evaluated. The results show that (1) the expression of Barnase is induced in rice leaves when inoculated with the spores of Magnaporthe grisea; (2) the induced expression level of Barnase surpasses the level of Barstar, which elicits a similar hypersensitive response (HR) in the leaves, and the transgenic plant shows high resistance to the rice fungal blast disease; and (3) transgenic rice plants also show obvious re-sistance to rice bacterial blight disease. Taken together, these results suggest that the transgenic rice plants harboring this two-component system acquire relatively broad spectrum resistance against pathogens, especially high resistance to rice fungal pathogen.

关键词：转基因水稻水稻病害抗病性受控细胞死亡致病病原体基因工程核糖核酸酶

An anther-specific chalcone synthase-like gene D5 related to rice pollen development

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Chinese Science Bulletin 2000年第21期45卷 1921-1926页

作者： Honghong Zheng lijia qu Meihua Liu Yi Zhang Yunping Shen Junming Wei Naisui Pan Hongya Gu Zhangliang Chen Beijing Univ Natl Lab Prot Engn & Plant Genet Engn Beijing 100871 Peoples R China

It was shown in a previous analysis that D5 gene from rice (Oryza sativa L.) was an anther-specific gene encoding a chalcone synthase-related protein. In this study, D5 gene was found specifically expressed in tapetum cells as well as in the peripheral cells of the vascular bundle of rice anthers by RNA in situ hybridization. In order to study its function, D5 was transformed into rice in both sense and antisense directions driven by a rice Actin 1 promoter. It has been observed that the pollen grains from the antisense D5 transgenic rice plants are abnormal, indicating that D5 plays a critical role in rice pollen development.

关键词： antisense in situ hybridization pollen development Oryza sativa.