检索结果-南通市图书馆

The deubiquitylase UCHL3 maintains cancer stem-like properties by stabilizing the aryl hydrocarbon receptor

Signal Transduction and Targeted Therapy 2020年第1期5卷 1609-1622页

作者： lianlian ouyang Bin Yan Yating Liu Chao Mao Min Wang Na Liu Zuli Wang Shouping Liu Ying Shi Ling Chen Xiang Wang Yan Cheng Ya Cao Desheng Xiao Lingqiang Zhang Shuang Liu Yongguang Tao Department of Oncology Institute of Medical SciencesNational Clinical Research Center for Geriatric DisordersXiangya HospitalCentral South University410008 ChangshaHunanChina Key Laboratory of Carcinogenesis and Cancer Invasion Ministry of EducationDepartment of PathologyXiangya HospitalCentral South University410078 ChangshaHunanChina Key Laboratory of Carcinogenesis of the Ministry of Health Cancer Research InstituteSchool of Basic MedicineCentral South University410078 ChangshaHunanChina Department of Thoracic Surgery Hunan Key Laboratory of Tumor Models and Individualized MedicineSecond Xiangya HospitalCentral South UniversityChangshaChina Xiangya School of Pharmaceutical Sciences Central South University410008 ChangshaChina Department of Pathology Xiangya HospitalCentral South University410008 ChangshaHunanChina State Key Laboratory of Proteomics National Center for Protein Sciences(Beijing)Beijing Institute of Lifeomics100850 BeijingChina

Cancer stem cells(CSCs)exhibit highly aggressive and metastatic features and resistance to chemotherapy and radiotherapy.Aryl hydrocarbon receptor(AhR)expression varies among non-small cell lung cancers(NSCLCs),and the mechanisms that support abnormal AhR expression in CSCs remain elusive.Here,we identified ubiquitin carboxyl terminal hydrolase L3(UCHL3),a DUB enzyme in the UCH protease family,as a bona fide deubiquitylase of the AhR in NSCLC.UCHL3 was shown to interact with,deubiquitylate,and stabilize AhR in a manner dependent on its deubiquitylation activity.Moreover,we showed that UCHL3 promotes the stem-like characteristics and potent tumorigenic capacity of NSCLC cells.UCHL3 increased AhR stability and the binding of AhR to the promoter regions of the“stemness”genes ATP-binding cassette subfamily G member 2(ABCG2),KLF4,and c-Myc.Depletion of UCHL3 markedly downregulated the“stemness”genes ABCG2,KLF4,and c-Myc,leading to the loss of selfrenewal and tumorigenesis in NSCLCs.Furthermore,the UCHL3 inhibitor TCID induced AhR degradation and exhibited significantly attenuated efficacy in NSCLC cells with stem cell-like properties.Additionally,UCHL3 was shown to indicate poor prognosis in patients with lung adenocarcinoma.In general,our results reveal that the UCHL3 deubiquitylase is pivotal for AhR protein stability and a potential target for NSCLC-targeted therapy.

关键词： lung markedly chemotherapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The cross-talk between methylation and phosphorylation in lymphoid-specific helicase drives cancer stem-like properties

引用

Signal Transduction and Targeted Therapy 2020年第1期5卷 670-683页

作者： Na Liu Rui Yang Ying Shi Ling Chen Yating Liu Zuli Wang Shouping Liu lianlian ouyang Haiyan Wang Weiwei Lai Chao Mao Min Wang Yan Cheng Shuang Liu Xiang Wang Hu Zhou Ya Cao Desheng Xiao Yongguang Tao Department of Pathology Key Laboratory of Carcinogenesis and Cancer Invasion(Ministry of Education)Xiangya HospitalCentral South University410078 HunanChina NHC Key Laboratory of Carcinogenesis(Central South University) Cancer Research Institute and School of Basic MedicineCentral South University410078 ChangshaHunanChina Postdoctoral Research Workstation Department of NeurosurgeryXiangya HospitalCentral South University410078 HunanChina Department of Oncology Institute of Medical SciencesNational Clinical Research Center for Geriatric DisordersXiangya HospitalCentral South University410008 ChangshaHunanChina Xiangya School of Pharmaceutical Sciences Central South University410078 ChangshaChina Hunan Key Laboratory of Tumor Models and Individualized Medicine Hunan Key Laboratory of Early Diagnosis and Precision Therapy in Lung CancerDepartment of Thoracic SurgerySecond Xiangya HospitalCentral South University410011 ChangshaChina Shanghai Institute of Material Medical Chinese Academy of Sciences(CAS)555 Zuchongzhi RoadZhangjiang Hi-Tech Park201203 ShanghaiChina

Posttranslational modifications(PTMs)of proteins,including chromatin modifiers,play crucial roles in the dynamic alteration of various protein properties and functions including stem-cell properties.However,the roles of Lymphoid-specific helicase(LSH),a DNA methylation modifier,in modulating stem-like properties in cancer are still not clearly clarified.Therefore,exploring PTMs modulation of LSH activity will be of great significance to further understand the function and activity of LSH.Here,we demonstrate that LSH is capable to undergo PTMs,including methylation and phosphorylation.The arginine methyltransferase PRMT5 can methylate LSH at R309 residue,meanwhile,LSH could as well be phosphorylated by MAPK1 kinase at S503 residue.We further show that the accumulation of phosphorylation of LSH at S503 site exhibits downregulation of LSH methylation at R309 residue,which eventually promoting stem-like properties in lung cancer.Whereas,phosphorylation-deficient LSH S503A mutant promotes the accumulation of LSH methylation at R309 residue and attenuates stem-like properties,indicating the critical roles of LSH PTMs in modulating stem-like properties.Thus,our study highlights the importance of the crosstalk between LSH PTMs in determining its activity and function in lung cancer stem-cell maintenance.

关键词： cancer residue phosphorylation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

糖尿病足风险预测模型的构建与验证

引用

护理学杂志 2022年第11期37卷 9-14页

作者：谢晓冉徐蓉张静陶静边旭娜高莲莲欧阳兰欣华中科技大学同济医学院附属同济医院护理部湖北武汉430030 华中科技大学同济医学院附属同济医院内分泌科湖北武汉430030 华中科技大学同济医学院护理学院

目的构建2型糖尿病患者糖尿病足风险预测模型,为医护人员识别高危患者并制订针对性干预方案提供参考。方法回顾性收集2018年3月至2020年3月住院2型糖尿病患者的临床资料,使用LASSO回归和多因素logistic回归分析筛选糖尿病足发生的危险因... 详细信息

目的构建2型糖尿病患者糖尿病足风险预测模型,为医护人员识别高危患者并制订针对性干预方案提供参考。方法回顾性收集2018年3月至2020年3月住院2型糖尿病患者的临床资料,使用LASSO回归和多因素logistic回归分析筛选糖尿病足发生的危险因素,建立2型糖尿病患者糖尿病足风险预测的列线图模型。计算C统计量评价模型的区分度,使用校准图和Brier分数评估模型的校准度。使用Bootstrap法进行内部验证,随后收集2020年4~12月住院2型糖尿病患者资料用于外部验证。结果开发数据集共纳入1527例患者,有319例(20.89%)患糖尿病足。最终9个预测因子进入模型:文化程度、溃疡史或截肢史、视网膜病变、是否注射胰岛素、震动感觉阈值、足背动脉搏动、足部皮肤异常变化、足部真菌感染和足部畸形。预测模型内部验证前后的C统计量分别为0.966和0.963,Brier分数分别为0.052和0.054,校准图中实际曲线和理想曲线重合度较好。外部验证数据集共纳入451例患者,有71例(15.74%)患糖尿病足。模型外部验证的C统计量为0.928,Brier分数为0.051,校准图显示模型的预测结果和观察结果之间一致性较好。结论构建的2型糖尿病患者糖尿病足风险预测模型具有良好的区分度和校准度,可为临床筛查糖尿病足高危患者提供借鉴。

关键词： 2型糖尿病糖尿病足足溃疡预测模型内部验证外部验证区分度校准度

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：