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Perfluorooctane sulfonate promotes the migration of colorectal cancer cells by inducing epithelial-mesenchymal transition

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Journal of Environmental Sciences 2024年第11期145卷 1-12页

作者： Fangfang Li Lu Chen Bi-Xia Peng Yuyang Lei Minjie Li liang-hong guo Institute of Environmental and Health Sciences China Jiliang UniversityHangzhou 310018China Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province Hangzhou 310015China College of Quality and Safety Engineering China Jiliang UniversityHangzhou 310018China College of Life Sciences China Jiliang UniversityHangzhou 310018China

The potential association between colorectal cancer(CRC)and environmental pollutants is *** studies have found that some perfluoroalkyl acids,including perfluorooctane sulfonate(PFOS),induced colorectal tumors in experimental animals and promoted the migration of and invasion by CRC cells in vitro,but the underlying mechanism is ***,we investigated the effects of PFOS on the proliferation and migration of CRC cells and the potentialmechanisms involving activating the PI3K/Akt-NF-κB signal pathway and epithelial-mesenchymal transition(EMT).It was found that PFOS promoted the growth andmigration of HCT116 cells at non-cytotoxic concentrations and increased the mRNA expression of the migration-related angiogenic cytokines vascular endothelial growth factor(VEGF)and interleukin-8(IL-8).In a mechanistic investigation,the up-stream signal pathway PI3K/Akt-NF-κB was activated by PFOS,and the process was suppressed by LY294002(PI3K/Akt inhibitor)and BAY11-7082(NF-κB inhibitor)respectively,leading to less proliferation of HCT116 ***,matrix metalloproteinases(MMP)and EMT-related markers were up-regulated after PFOS exposure,and were also suppressed respectively by LY294002 and ***,the up-regulation of EMT markers was suppressed by a MMP inhibitor *** together,our results indicated that PFOS promotes colorectal cancer cell migration and proliferation by activating the PI3K/Akt-NF-κB signal pathway and epithelial-mesenchymal *** could be a potential toxicological mechanism of PFOS-induced malignant development of colorectal cancer.

关键词： Perfluorooctane sulfonate Colorectal cancer Cell migration PI3K/Akt-NF-κB Epithelial-mesenchymal transition

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Haze and health

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National Science Review 2016年第4期3卷 412-413页

作者： liang-hong guo Research Center for Eco-environmental Science Chinese Academy of Sciences

As a general term,haze is defined as an aggregation in the atmosphere of very fine,widely dispersed,solid or liquid particles,or both,giving the air an opalescent appearance that subdues colors[1].While natural haze such as that in the forestcovered mountains and valleys has long existed,hazardous haze derived primarily from the combustion of fossil-fuel products has evolved with human activities.

关键词： PM Haze and health very

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Research on Feature Extraction of Remnant Particles of Aerospace Relays

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Chinese Journal of Aeronautics 2007年第3期20卷 253-259页

作者： WANG Shu-juan GAO hong-liang ZHAI guo-fu Department of Electrical Engineering Harbin Institute of Technology Harbin 150001 China

The existence of remnant particles, which significantly reduce the reliability of relays, is a serious problem for aerospace relays. The traditional method for detecting remnant particles-particle impact noise detection （PIND）-can be used merely to detect the existence of the particle; it is not able to provide any information about the particles＇ material. However, information on the material of the particles is very helpful for analyzing the causes of remnants. By analyzing the output acoustic signals from a PIND tester, this paper proposes three feature extraction methods： unit energy average pulse durative time, shape parameter of signal power spectral density （PSD）, and pulse linear predictive coding coefficient sequence. These methods allow identified remnants to be classified into four categories based on their material. Furthermore, we prove the validity of this new method by processing P1ND signals from actual tests.

关键词： feature extraction aerospace relays remnant particles particle impact noise detection

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In vitro fluorescence displacement investigation of thyroxine transport disruption by bisphenol A

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Journal of Environmental Sciences 2011年第2期23卷 315-321页

作者： Jie Cao liang-hong guo Bin Wan Yin Wei State Key Laboratory of Environmental Chemistry and Ecoloxicology Research Center for Eco-environmental Sciences Chinese Academy of Sciences Beijing 100085 China

Bisphenol A （BPA） is a chemical with high production volume and wide applications in many industries. Although BPA is known as an endocrine disruptor, its toxic mechanisms have not been fully characterized. Due to its structural similarity to thyroid hormones thyroxine （T4） and triiodothyronine （T3）, one possible mechanism of BPA toxicity is disruption of hormone transport by competitive binding with the transport proteins. In this study, the binding interactions of BPA, T4, and T3 with three thyroid hormone transport proteins, human serum albumin （HSA）, transthyretin （TTR）, and thyroxine-binding globulin （TBG） were investigated by fluorescence measurement. Using two site-specific fluorescence probes dansylamide and dansyl-L-proline, the binding constants of BPA with HSA at drug site I and site II were determined as 2.90 × 10^4 and 3.14 × 10^4 L/mol, respectively. By monitoring the intrinsic fluorescence of tryptophan, a binding constant of 4.70 ×10^3 L/mol was obtained. Similarly, by employing 8-anilino-l-naphthalenesulfonic acid as fluorescence probe, the binding affinity of BPA with TTR and TBG was measured to be 3.10 × 10^5 and 5.90× 10^5 L/mol, respectively. In general, BPA showed lower binding affinity with the proteins than T3 did, and even lower affinity than T4. Using these binding constants, the amount of BPA which would bind to the transport proteins in human plasma was estimated. These results suggest that the concentrations of BPA commonly found in human plasma are probably not high enough to interfere with T4 transport.

关键词： bisphenol A human serum albumin human transthyretin human thyroxine-binding globulin fluorescence displacement method

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Cytotoxicity and autophagy induction by graphene quantum dots with different functional groups

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Journal of Environmental Sciences 2019年第3期31卷 198-209页

作者： Yichun Xie Bin Wan Yu Yang Xuejing Cui Yan Xin liang-hong guo State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences University of Chinese Academy of Sciences CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology of China Institute of Environment and Health Jianghan University

Graphene quantum dots(GQDs) possess great potential in various applications due to their superior physicochemical properties and wide array of available surface ***, the toxicity of GQDs has not been systematically assessed, thus hindered their further development; especially, the risk of surface modifications of GQDs is largely unknown. In this study, we employed a lung carcinoma A549 cells as the model to investigate the cytotoxicity and autophagy induction of three types GQDs, including cGQDs(COOH-GQDs), hGQDs(OH-GQDs), and aGQDs(NH_2-GQDs). The results showed hGQDs was the most toxic, as significant cell death was induced at the concentration of 100 μg/mL,determining by WST-1 assay as well as Annexin-V-FITC/PI apoptosis analysis, whereas cGQDs and aGQDs were non-cytotoxic within the measured concentration. Autophagy detection was performed by TEM examination, LC3 fluorescence tracking, and Westernblot. Both aGQDs and hGQDs induced cellular autophagy to various degrees except for cGQDs. Further analysis on autophagy pathways indicated all GQDs significantly activated p-p38 MAPK; p-ERK1/2 was inhibited by aGQDs and hGQDs but activated by c GQDs. p-JNK was inhibited by aGQDs and c GQDs, while activated by hGQDs. Simultaneously, Akt was activated by hGQDs but inhibited by aGQDs. Inhibition of autophagy by 3-MA significantly increased the cytotoxicity of GQDs, suggesting that autophagy played a protective role against the toxicity of GQDs. In conclusion, c GQDs showed excellent biocompatibility and may be considered for biological applications. Autophagy induction may be included in the health risk assessment of GQDs as it reflects the stress status which may eventually lead to diseases.

关键词： Graphene quantum dots Cytotoxicity Autophagy Surface functional group

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Processing of a low-costγ-γ'NiPtAl coating with improved oxidation resistance

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Rare Metals 2024年第6期43卷 2832-2841页

作者： Yan Jiang liang-liang Wei Jian He hong-Bo guo School of Materials Science and Engineering Beihang UniversityBeijing 100191China Key Laboratory of High-Temperature Structural Materials and Protective Coatings Ministry of Industry and Information TechnologyBeihang UniversityBeijing 100191China

A novelγ-γ'(Ni solid solution—Ni_(3)Al)NiPtAl coating was produced on advanced single-crystal(SC)superalloy N5 by electroplating of a thin Pt coating with~3μm in thickness followed by heat treatment at 1000℃in *** comparison,a traditionalγ-γ'NiPtAl coating was also produced by electroplating of a thick Pt coating with~6μm in thickness followed by heat treatment at 1100℃in *** novel coating has a mass gain of~0.5 mg·cm^(-2)after 100 h 1-h cyclic oxidation at 1150℃,showing better oxidation resistance than the traditional *** oxide scale mostly consisting ofα-Al_(2)O_(3)grew on the novel coating because the element Cr from the SC alloy substrate contributes to the formation ofα-Al_(2)O_(3).Pt content in the novel coating has significant effect on the oxidation *** optimized Pt content in the coating is about 16 at%,which is helpful to reduce the diffusion of harmful elements from the substrate into the coating but also to reduce the cost of the coating.

关键词： γ-γ'NiPtAl coating Platinum Oxidation Al_(2)O_(3) Superalloy

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Development of indirect competitive fluorescence immunoassay for 2,2′,4,4′-tetrabromodiphenyl ether using DNA/dye conjugate as antibody multiple labels

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Journal of Environmental Sciences 2012年第7期24卷 1334-1340页

作者： Zi-Yan Fan Young Soo Keum Qing-Xiao Li Weilin L.Shelver liang-hong guo State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-environment SciencesChinese Academy of SciencesBeijing 100085China College of Life and Environmental Sciences Konkuk University in Korea1 Hwayang-dongGwangjin-guSeoul 143-701Korea Department of Molecular Bioscience and Bioengineering University of Hawaii at Manoa1955 East-West RoadHonoluluHI 96822USA USDA-ARS Bioscience Research Laboratory 1605 Albrechht BoulevardFargoND 58105USA

An indirect competitive fluorescence immunoassay using a DNA/dye conjugate as antibody multiple labels was developed on 96-well plates for the identification and quantification of 2,2＇,4,4＇-tetrabromodiphenyl ether （BDE-47） in aqueous samples. A hapten, 2,4,2＇- tribromodiphenyl ether-4＇-aldehyde, was synthesized, and was conjugated to bovine serum albumin to form a coating antigen. Specific recognition of the antigen by anti-PBDE antiserum was confirmed by a surface plasmon resonance measurement. In the immunoassay, the coating antigen was adsorbed on a 96-well plate first, and a sample, antiserum and biotinylated goat anti-rabbit secondary antibody were then added and reacted sequentially. A biotinylated, double-stranded DNA with 219 base pairs was attached to the secondary antibody by using streptavidin as a molecular bridge. In situ multiple labeling of the antibody was accomplished after addition of a DNA-binding fluorescent dye, SYBR Green I. The working range of the immunoassay for the BDE-47 standard was 3.1-390 ~tg/L, with an IC50 value of 15.6 Ixg/L. The calculated LOD of the immunoassay is 0.73 Ixg/L. The immunoassay demonstrated relatively high selectivity for BDE-47, showing very low cross-reactivity （〈 3%） with BDE-15, BDE-153 and BDE-209. With a spiked river water sample containing 50 Izg/L BDE-47, quantification by the immunoassay was 41.9 ~tg/L, which compared well with the standard GC-ECD method （45.7 Ixg/L）. The developed immunoassay provides a rapid screening tool for polybrominated diphenyl ethers in environmental samples.

关键词： polybrominated diphenyl ethers fluorescence immunoassay microplate multiple labeling

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Influence of Bath Composition on Magnetic Properties of Electrodeposited Co-Pt-W Thin Films

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Journal of Iron and Steel Research International 2007年第4期14卷 65-68页

作者： GE hong-liang WEI guo-ying WU Qiong ZHOU Qiao-ying WANG Xin-yan College of Material Science and Engineering China Jiliang University Hangzhou 310018 Zhejiang China

Effect of bath composition （[Co^2＋]/[-Pt^Ⅳ ] and [-WO4^2- ], [cit^-]） and pH on the magnetic properties of electrodeposited Co-Pt-W thin films has been investigated. Electrodeposited Co-Pt-W thin films exhibited strong perpendicular magnetic anisotropy when the ratio of [-Co^2＋ ] to [-Pt^Ⅳ ] was 10 ; cathode current efficiency and perpendicular magnetic anisotropy showed little variations when [WO4^2- ] was lower than 0. 1 mol/L, but perpendicular magnetic anisotropy had strengthened when [WO4^2-] was over 0. 1 mol/L, which could be explained by the fact that the hydrogen evolution could produce pores as magnetic domain pinnings; citrate as complexing reagent can promote the polarization of [Co^2＋] and [Pt^Ⅳ]. As a result, the equilibrium electrode potentials of cobalt and platinum moved to negative direction, which led to the co-deposition of Co, Pt, and W. It was also found out that the as-deposited Co- Pt-W hard magnetic thin films were very homogeneous, smooth, and had the maximum coercivity for the bath pH 8. 5 and the concentration of citrate 0. 26 mol/L.

关键词： electrodeposition Co-Pt-W thin film magnetic property bath composition

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Unprecedented Two‑Step Chemiluminescence of Polyamine‑Functionalized Carbon Nanodots Induced by Fenton‑Like System

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Journal of Analysis and Testing 2017年第4期1卷 315-321页

作者： Fanglan Geng Lixia Zhao Yuehui Kang liang‑hong guo State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco‑Environmental SciencesChinese Academy of SciencesP.O.Box 2871Beijing 100085People’s Republic of China

We reported an unprecedented chemiluminescence(CL)behavior of polyamine-functionalized carbon dots induced by Fe^(3+)–H_(2)O_(2) Fenton-like *** firststep CL intensity increased with the increasing of the concentration of H_(2)O_(2) and Fe^(3+),when the Fe^(3+)concentration came to 10^(−3) M,the unprecedented two-step CL behavior *** CL intensity of BPEI-CDs induced by Fenton-like system was about 10 times stronger than that of naked *** possible two-step CL mechanism was speculated based on the photoluminescence spectra,CL emission spectra,and the effects of radical scavengers on the CL *** recombination of the injected holes by strong oxidant perferrate formed through Fe^(3+)–H_(2)O_(2) reaction and the;OH generated from successive Fenton reaction with the thermally excited electrons was proposed,which further facilitate full understanding about the optical properties of carbon dots.

关键词： Polyamine-functionalized carbon nanodots Two-step chemiluminescence Fenton-like system Fe^(3+)-H_(2)O_(2)reaction

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Imbalanced rock burst assessment using variational autoencoder-enhanced gradient boosting algorithms and explainability

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Underground Space 2024年第4期17卷 226-245页

作者： Shan Lin Zenglong liang Miao Dong hongwei guo hong Zheng Key Laboratory of Urban Security and Disaster Engineering Ministry of EducationBeijing University of TechnologyBeijing 100124China Department of Civil and Environmental Engineering The Hong Kong Polytechnic University(PolyU)Hung HomKowloon 999077Hong KongChina

We conducted a study to evaluate the potential and robustness of gradient boosting algorithms in rock burst assessment,established a variational autoencoder(VAE)to address the imbalance rock burst dataset,and proposed a multilevel explainable artificial intelligence(XAI)tailored for tree-based ensemble *** collected 537 data from real-world rock burst records and selected four critical features contributing to rock burst ***,we employed data visualization to gain insight into the data’s structure and performed correlation analysis to explore the data distribution and feature ***,we set up a VAE model to generate samples for the minority class due to the imbalanced class *** conjunction with the VAE,we compared and evaluated six state-of-theart ensemble models,including gradient boosting algorithms and the classical logistic regression model,for rock burst *** results indicated that gradient boosting algorithms outperformed the classical single models,and the VAE-classifier outperformed the original classifier,with the VAE-NGBoost model yielding the most favorable *** to other resampling methods combined with NGBoost for imbalanced datasets,such as synthetic minority oversampling technique(SMOTE),SMOTE-edited nearest neighbours(SMOTE-ENN),and SMOTE-tomek links(SMOTE-Tomek),the VAE-NGBoost model yielded the best ***,we developed a multilevel XAI model using feature sensitivity analysis,Tree Shapley Additive exPlanations(Tree SHAP),and Anchor to provide an in-depth exploration of the decision-making mechanics of VAE-NGBoost,further enhancing the accountability of treebased ensemble models in predicting rock burst occurrences.

关键词： Gradient boosting VAE Ensemble learning Explainable artificial intelligence(XAI) Rock burst

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