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Metabolomic-based analysis reveals bile acid-mediated ovarian failure induced by low temperature in zebrafish

Zoological Research 2024年第4期45卷 791-804页

作者： Wen-Hao Li Zhi-Qiang Li Meng-Di Bu Jia-Zhen Li liang-biao chen Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationCollege of Fisheries and Life SciencesShanghai Ocean UniversityShanghai 201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdaoShandong 266237China International Research Center for Marine Biosciences Ministry of Science and TechnologyCollege of Fisheries and Life SciencesShanghai Ocean UniversityShanghai 201306China

As ectotherms, fish are highly sensitive to temperature fluctuations, which can profoundly impact their reproductive cycles. In this study, we investigated the fertility and histological characteristics of zebrafish(Danio rerio) ovaries exposed to a temperature gradient ranging from the thermopreferendum temperature of the species,27℃, to lower temperatures of 22℃, 20℃, and 13℃ over a period of two weeks. Comparative metabolomic(six biological replicates for each temperature) and transcriptomic(four biological replicates for each temperature) analyses were conducted under the four temperature conditions. Results indicated that lower temperatures inhibited oocyte development and differential metabolites were involved in steroid hormone production,antioxidant function, and lipid and protein *** reproductive hormones, increased proteolysis,and lipid degradation significantly impeded oocyte development and egg maturation. Notably, a significant increase in bile acid content was noted in the ovaries of the cold-treated fish, indicating that bile acids play a critical role in ovarian failure. Overall, these findings provide valuable insights into the mechanisms governing the reproductive response of fish to cold stress.

关键词： old stress Ovarian failure Metabolome Zebrafish

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Study on Effectiveness of Solitary-Wave-Like Form Based on Overtopping Data from Irregular Wave Tests

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China Ocean Engineering 2024年第4期38卷 701-710页

作者： LI Xiao-liang Huang Gui-biao School of Naval Architecture&Ocean Engineering Jiangsu University of Science and TechnologyZhenjiang 212100China

The previous study conducted by Li(2022)demonstrates that the Goda graph can be adequately represented by a solitary-wave-like form across the entire range of relative water depth,with the exception of the extremely shallow ***,it remains uncertain whether this form is equally effective when applied to test data generated by irregular waves,as the Goda graph was initially developed based on overtopping data from regular wave ***,it is unclear whether this form is suitable for formulating overtopping discharge at composite vertical *** order to address these questions,a 2D overtopping experiment was conducted,incorporating both simple and composite types of vertical walls,with various relative water depths across the entire range,excluding the extremely shallow zone.A novel analysis procedure was developed,which proved to be highly productive and can be considered a general method for data ***,the study yielded two conclusions:(1)the solitary-wave-like form is remarkably effective in formulating overtopping test data generated by irregular waves,regardless of whether the vertical wall is simple or composite,and(2)the resulting formulas exhibit definitely better performance compared with existing formulas.

关键词： solitary-wave-like form overtopping discharge overtopping formula vertical wall

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Dusp 1 regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity

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Zoological Research 2023年第1期44卷 126-141页

作者： Ying Wang Hua-Min Wang Yan Zhou Ling-Hong Hu Jing-Ming Wan Ji-Hui Yang Hong-Bo Niu Xiu-Ping Hong Peng Hu liang-biao chen International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai 200120China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai 200120China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai 200120China

Temperature tolerance restricts the distribution of a species. However, the molecular and cellular mechanisms that set the thermal tolerance limits of an organism are poorly understood. Here, we report on the function of dual-specificity phosphatase 1(DUSP1) in thermal tolerance regulation. Notably, we found that dusp1-/- zebrafish grew normally but survived within a narrowed temperature range. The higher susceptibility of these mutant fish to both cold and heat challenges was attributed to accelerated cell death caused by aggravated mitochondrial dysfunction and over-production of reactive oxygen species in the gills. The DUSP1-MAPK-DRP1 axis was identified as a key pathway regulating these processes in both fish and human cells. These observations suggest that DUSP1 may play a role in maintaining mitochondrial integrity and redox homeostasis. We therefore propose that maintenance of cellular redox homeostasis may be a key mechanism for coping with cellular thermal stress and that the interplay between signaling pathways regulating redox homeostasis in the most thermosensitive tissue(i.e., gills) may play an important role in setting the thermal tolerance limit of zebrafish.

关键词： Redox homeostasis Zebrafish Ectothermal Mitochondrial fission Thermal stress MAPK

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Non-interactive SM2 threshold signature scheme with identifiable abort

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Frontiers of Computer Science 2024年第1期18卷 171-185页

作者： Huiqiang liang Jianhua chen School of Mathematics and Statistics Wuhan UniversityWuhan 430072China

A threshold signature is a special digital signature in which the N-signer share the private key x and can construct a valid signature for any subset of the included t-signer,but less than t-signer cannot obtain any *** the breakthrough achievements of threshold ECDSA signature and threshold Schnorr signature,the existing threshold SM2 signature is still limited to two parties or based on the honest majority setting,there is no more effective solution for the multiparty *** make the SM2 signature have more flexible application scenarios,promote the application of the SM2 signature scheme in the blockchain system and secure cryptocurrency *** paper designs a non-interactive threshold SM2signature schemebasedon partially homomorphic encryption and zero-knowledge *** the last round requires the message input,so make our scheme non-interactive,and the pre-signing process takes 2 rounds of communication to complete after the key *** allow arbitrary threshold t

关键词： SM2 signature secure multi-party computation threshold signature UC-secure dishonest majority

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The E3 ubiquitin ligase TRAF6 controls CTLA-4 turnover and promotes T-cell-mediated antitumor immunity

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Cellular & Molecular Immunology 2024年第1期21卷 97-99页

作者： Xiao-liang Jie Hua Guo Guang-biao Zhou State Key Laboratory of Molecular Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical College100021 BejingChina

Yu et al.[1]reported that the tumor necrosis factor(TNF)receptor OX40(CD134)-TNF receptor-associated factor 6(TRAF6)axis mediates Lys63-linked ubiquitination and subsequent lysosomal degradation of cytotoxic T-lymphocyte antigen 4(CTLA-4),leading to the enhancement of CD8^(+)T-cell-mediated antitumor *** findings unveil a previously unknown mechanism in controlling the stability of the immune checkpoint CTLA-4 and provide a potential therapeutic target for the improvement of Tcell-based immunotherapy.

关键词： immunity OX40 ubiquitin

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Devonian integrative stratigraphy,biotas,and paleogeographical evolution of the Qinghai-Tibetan Plateau and its surrounding areas

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Science China Earth Sciences 2024年第4期67卷 1036-1070页

作者： Wenkun QIE Kun liang Wen GUO biao GAO Junjun SONG Bo chen Pu HUANG Li QIAO Honghe XU Jitao chen Yucong SUN Yichun ZHANG State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and PalaeontologyChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Nanjing 211135China

The Devonian System in the Qinghai-Tibetan Plateau and its surrounding areas is widely distributed,diverse in lithotypes and rich in *** records the crucial processes of continuous subduction and reduction of the Paleo-Asian Ocean in the northern hemisphere and the transformation of the Proto-Tethys Ocean into the Paleo-Tethys Ocean in the southern ***,it is of great significance for explaining the global paleogeographic evolution,tectonic activities and biodiversity changes during this critical *** blocks on both sides of the southern Tienshan suture zone and the Longmu Co-ShuanghuChangning-Menglian suture zone belong to different sedimentary systems and paleobiogeographic realms and regions,showing important tectonic paleogeographic partitioning *** two suture zones represent the main branches of the PaleoAsian Ocean and the Paleo-Tethys Ocean,respectively,and on this basis the Devonian System can be subdivided into three types:the Tienshan type,the Tethys type and the Gondwana *** on recent research progress on the Devonian stratigraphy and paleontology in combining provenance analysis and biotic characteristics in the Qinghai-Tibetan Plateau and surrounding areas,this paper aims to establish the latest integrative stratigraphic framework and conduct paleogeographic reconstruction,providing foundation for exploring the geological evolution and dynamic mechanisms of various terranes and blocks in the northern margin of *** results indicate that the North Qiangtang,South Qiangtang,Simao,Baoshan and Tengchong terranes,as well as the Himalaya Tethys Zone and the South China Block have affinities with the Indian Block,while the Lhasa Terrane has affinities with the Australian Block,and may be located between the South China and Australian *** the Emsian(Early Devonian),the South China Block separated from the northern margin of Gondwana,a process that was accompanied by the opening of the Jinshajiang-Ailaosh

关键词： Devonian Paleo-Tethys Ocean Integrative stratigraphy Biotas Provenance Analysis Paleogeography

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The effect of glutathione on glucosinolate biosynthesis through the sulfur assimilation pathway in pakchoi associated with the growth conditions

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Horticultural Plant Journal 2024年第2期10卷 473-487页

作者： biao Zhu Zhile liang Dan Wang Chaochao He Zhujun Zhu Jing Yang Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable Ministry of Agriculture and Rural AffairsCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang ProvinceCollege of Horticulture ScienceZhejiang A&F UniversityHangzhouZhejiang 311300China

Glucosinolates(GSLs) are a group of nitrogen-and sulfur-containing secondary metabolites, synthesized primarily in members of the Brassicaceae family, that play an important role in food flavor, plant antimicrobial activity, resistance to insect attack, stress tolerance, and human anti-cancer effects. As a sulfur-containing compound, glutathione has a strong connection with GSLs biosynthesis as a sulfur donor or redox system, and exists in reduced(glutathione;GSH) and oxidized(glutathione disulfide;GSSG) forms. However, the mechanism of GSH regulating GSLs biosynthesis remainds unclear. Hence, the exogenous therapy to pakchoi under normal growth condition and sulfur deficiency condition were conducted in this work to explore the relevant mechanism. The results showed that exogenous application of buthionine sulfoximine, an inhibitor of GSH synthesis, decreased the transcript levels of GSLs synthesis-related genes and transcription factors, as well as sulfur assimilation-related genes under the normal growth condition. Application of exogenous GSH inhibited the expression of GSLs synthesis-and sulfur assimilation-related genes under the normal condition, while the GSLs biosynthesis and the sulfur assimilation pathway were activated by exogenous application of GSH when the content of GSH in vivo of plants decreased owing to sulfur deficiency. Moreover,exogenous application of GSSG increased the transcript levels of GSLs synthesis-and sulfur assimilation-related genes under the normal growth condition and under sulfur deficiency. The present work provides new insights into the molecular mechanisms of GSLs biosynthesis underlying glutathione regulation.

关键词： Pakchoi Glucosinolates Reduced glutathione Oxidized glutathione Sulfur assimilation

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Surface integrity evolution during creep feed profile grinding ofγ-TiAl blade tenon

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Chinese Journal of Aeronautics 2024年第8期37卷 496-512页

作者： Tao chen Xiaowei WANG biao ZHAO Wenfeng DING Jiuhua XU National Key Laboratory of Science and Technology on Helicopter Transmission Nanjing University of Aeronautics and AstronauticsNanjing 210016China

Gamma titanium-aluminum(γ-TiAl)intermetallic compounds are increasingly used in manufacturing key hot-end components(e.g.,blade tenon)in aero engines due to their high specific strength and lightweight *** feed profile grinding(CFPG)as a crucial precision process that is applied to produce the final profile of the blade ***,sudden surface burns and microcracks of machined c-TiAl blade tenon often occur because of its low plasticity and high strength during grinding processes,leading to poor surface *** this work,CFPG experiments based on the profile characteristics ofγ-TiAl blade tenon were performed and an associated undeformed chip thickness model considering grain–workpiece contact condition was established to explore the evolution of the surface ***,the surface integrity was analyzed at different positions of the blade tenon in terms of surface roughness and morphology,metallographic structure,microhardness,and residual *** show that the profile characteristics of blade tenon have a significant influence on machined surface integrity because of the thermomechanical effect at various detecting *** residual stress was established based on the undeformed chip thickness model considering the profile structure,with a prediction error of 10%–15%.The thermomechanical effect is more obvious at the bottom area,where the surface roughness,work hardening degree,and subsurface plastic deformation range are the largest,while the values at the bevel area are the *** on the undeformed chip thickness model,a residual stress finite element simulation was conducted by employing thermomechanical coupled *** addition,the error between the simulation and the experiment was between 10%–15%.Strain and strain rate equations were established through the relationship between material displacement and *** average strain and strain rate of the ground surface when ap is 1.0 mm are 18.8%and 33.2%l

关键词： Gamma titanium-aluminum intermetallic compounds Blade tenon Creep feed profile grinding Surface integrity Thermomechanical effect

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Thermal Performance of a 4 K High-Frequency Pulse Tube Cryocooler with Different Working Fluids

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Journal of Thermal Science 2024年第4期33卷 1468-1479页

作者： GAO Zhaozhao YANG biao FAN Xiaoyu chen Liubiao WANG Junjie Key Laboratory of Cryogenic Science and Technology Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing100190China University of Chinese Academy of Sciences Beijing100049China

The high-frequency pulse tube cryocooler(HPTC)represents a promising miniature cryocooling technology due to its compact structure and the absence of low-temperature moving ***,limited to the non-ideal gas effect of4He,the HPTC is hard to obtain high cooling performance in the liquid helium temperature range.3He as the working fluid can effectively improve the cooling performance of the HPTC,but the high cost hinders its wide *** consideration of both cooling performance and cost-effectiveness,this paper explores the feasibility of utilizing^(3)He-^(4)He mixtures as the working fluid for ***,the experimental results of a developed HPTC based4He are *** a total power consumption of 575 W,the lowest temperature of 3.26 K was *** the measured cooling power at 4.2 K was 20.8 *** the theoretical utmost efficiency of the cryocooler was calculated in terms of the thermophysical properties of the working fluids,using ^(3)He-^(4)He mixtures with different compositions as the working *** whole machine modeling of the HPTC was further carried out,and the influence of the working fluids with different components on the structural parameters such as double-inlet and inertance tube,and operating parameters such as pressure and frequency were *** calculated results show that the cooling power is expected to be increased to36 mW and 53 mW if the equimolar ^(3)He-^(4)He mixture and pure ^(3)He are used,respectively.

关键词： high-frequency pulse tube cryocooler ^(3)He-^(4)He mixture liquid helium temperature thermally coupled

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AFM probe for measuring~10^(−5)ultra-low friction coefficient:Design and application

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Friction 2024年第1期12卷 64-73页

作者： Yushan chen liang JIANG Linmao QIAN Tribology Research Institute State Key Laboratory of Traction PowerSouthwest Jiaotong UniversityChengdu 610031China

Superlubricity provides a novel approach to addressing friction and wear issues in mechanical ***,little is known regarding improving the atomic force microscope(AFM)friction coefficient measurement ***,this study established the theoretical formula for the AFM friction coefficient measurement and deduced the measurement ***,the formula was applied to the AFM probe with a rectangular cross-section *** measurement resolution is associated with the dimensional properties of the AFM probe,the mechanical properties of the cantilever material,the properties of the position-sensitive detector(PSD),and probably the anti-vibration performance of the *** is feasible to make the cantilever as short as possible and the tip as high as possible to improve the measurement *** AFM probe for measuring an ultra-low friction coefficient was designed and *** cantilever’s length,width,and thickness are 50,35,and 0.6μm,*** tip height is 23μ*** measurement resolution can reach 7.1×10^(−6) under the maximum normal ***,the AFM probe was applied to measure the superlubricity between graphene *** friction coefficient is 0.00139 under 853.08 *** work provides a promising method for measuring a~10^(−5) friction coefficient of superlubricity.

关键词： atomic force microscope(AFM) superlubricity friction coefficientμ probe

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