检索结果-南通市图书馆

Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis

引用

Acta Pharmaceutica Sinica B 2024年第4期14卷 1624-1643页

作者： Can Cheng Hanhui Yao Heng Li Jingwen Liu Zhengyi Liu Yang Wu liang zhu Hejie Hu Zhengdong Fang liang Wu Department of General Surgery the First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery the First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Department of Comprehensive Surgery Anhui Provincial Cancer HospitalWest District of the First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Anhui Provincial Hospital Health Management Center the First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Department of Breast Surgery Henan Provincial People's HospitalPeople's Hospital of Zhengzhou UniversityPeople's Hospital of Henan UniversityZhengzhou 450003China

HMGA2,a pivotal transcription factor,functions as a versatile regulator implicated in the progression of diverse aggressive malignancies.In this study,mass spectrometry was employed to identify ubiquitin-specific proteases that potentially interact with HMGA2,and USP48 was identified as a deubiquitinating enzyme of HMGA2.The enforced expression of USP48 significantly increased HMGA2 protein levels by inhibiting its degradation,while the deprivation of USP48 promoted HMGA2 degradation,thereby suppressing tumor invasion and metastasis.We discovered that USP48 undergoes SUMOylation at lysine 258,which enhances its binding affinity to HMGA2.Through subsequent phenotypic screening of small molecules,we identified DUB-IN-2 as a remarkably potent pharmacological inhibitor of USP48.Interestingly,the small-molecule inhibitor targeting USP48 induces destabilization of HMGA2.Clinically,upregulation of USP48 or HMGA2 in cancerous tissues is indicative of poor prognosis for patients with colorectal cancer(CRC).Collectively,our study not only elucidates the regulatory mechanism of DUBs involved in HMGA2 stability and validates USP48 as a potential therapeutic target for CRC,but also identifies DUB-IN-2 as a potent inhibitor of USP48 and a promising candidate for CRC treatment.

关键词： Colorectal cancer Invasion and metastasis Post-translational modification Ubiquitination SUMOylation USP48 HMGA2 Specific inhibitors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Self-confocal NIR-II fluorescence microscopy for multifunctional in vivo imaging

引用

Journal of Innovative Optical Health Sciences 2024年第1期17卷 105-119页

作者： Jing Zhou Tianxiang Wu Runze Chen liang zhu Hequn Zhang Yifei Li Liying Chen Jun Qian State Key Laboratory of Modern Optical Instrumentations Centre for Optical and Electromagnetic ResearchCollege of Optical Science and EngineeringInternational Research Center for Advanced PhotonicsZhejiang UniversityHangzhou 310058P.R.China Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cell and Regenerative Medicine Zhejiang UniversityHangzhou 310058P.R.China College of Biomedical Engineering and Instrument Science Interdisciplinary Institute of Neuroscience and Technology(ZIINT)Zhejiang UniversityHangzhou 310027P.R.China

Fluorescence imaging in the second near-infrared window(NIR-II,900–1880 nm)with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imaging with high spatial resolution.However,the traditional NIR-IIfluorescence confocal microscope with separate excitation focus and detection pinhole makes it possess low confocal e±ciency,as well as di±cultly to adjust.Two types of upgraded NIR-IIfluorescence confocal microscopes,sharing the same pinhole by excitation and emission focus,leading to higher confocal e±ciency,are built in this work.One type is-ber-pinhole-based confocal microscope applicable to CW laser excitation.It is constructed forfluorescence intensity imaging with large depth,high stabilization and low cost,which could replace multiphotonfluorescence microscopy in some applications(e.g.,cerebrovascular and hepatocellular imaging).The other type is air-pinhole-based confocal microscope applicable to femtosecond(fs)laser excitation.It can be employed not only for NIR-IIfluorescence intensity imaging,but also for multi-channelfluorescence lifetime imaging to recognize different structures with similarfluorescence spectrum.Moreover,it can be facilely combined with multiphotonfluorescence microscopy.A single fs pulsed laser is utilized to achieve up-conversion(visible multiphotonfluorescence)and down-conversion(NIR-II one-photonfluorescence)excitation simultaneously,extending imaging spectral channels,and thus facilitates multi-structure and multi-functional observation.

关键词： Self-confocal fiber-pinhole air-pinhole multi-channe fluorescence lifetime imaging multi-color imaging

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The journey towards physiology and pathology: Tracing the path of neuregulin 4

引用

Genes & Diseases 2024年第2期11卷 687-700页

作者： Min Chen Jieying zhu Hongyang Luo Wangjing Mu liang Guo School of Exercise and Health and Shanghai Frontiers Science Research Base of Exercise and Metabolic Health Shanghai University of SportShanghai 200438China

Neuregulin 4 (Nrg4),an epidermal growth factor (EGF) family member,can bind to and activate the ErbB4 receptor tyrosine kinase.Nrg4 has five different isoforms by alternative splicing and performs a wide variety of functions.Nrg4 is involved in a spectrum of physiological processes including neurobiogenesis,lipid metabolism,glucose metabolism,thermogenesis,and angiogenesis.In pathological processes,Nrg4 inhibits inflammatory factor levels and suppresses apoptosis in inflammatory diseases.In addition,Nrg4 could ameliorate obesity,insulin resistance,and cardiovascular diseases.Furthermore,Nrg4 improves non-alcoholic fatty liver disease (NAFLD) by promoting autophagy,improving lipid metabolism,and inhibiting cell death of hepatocytes.Besides,Nrg4 is closely related to the development of cancer,hyperthyroidism,and some other diseases.Therefore,elucidation of the functional role and mechanisms of Nrg4 will provide a clearer view of the therapeutic potential and possible risks of Nrg4.

关键词： Angiogenesis Cardiovascular diseases Inflammation Insulin resistance Lipid and glucose metabolism NAFLD Neurobiogenesis Obesity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gut flora in multiple sclerosis:implications for pathogenesis and treatment

引用

Neural Regeneration Research 2024年第7期19卷 1480-1488页

作者： Weiwei Zhang Ying Wang Mingqin zhu Kangding Liu Hong-liang Zhang Department of Neurology the First Hospital of Jilin UniversityJilin UniversityChangchunJilin ProvinceChina Department of Life Sciences National Natural Science Foundation of ChinaBeijingChina

Multiple sclerosis is an inflammatory disorder chara cterized by inflammation,demyelination,and neurodegeneration in the central nervous system.Although current first-line therapies can help manage symptoms and slow down disease progression,there is no cure for multiple sclerosis.The gut-brain axis refers to complex communications between the gut flo ra and the immune,nervous,and endocrine systems,which bridges the functions of the gut and the brain.Disruptions in the gut flora,termed dys biosis,can lead to systemic inflammation,leaky gut syndrome,and increased susceptibility to infections.The pathogenesis of multiple sclerosis involves a combination of genetic and environmental factors,and gut flora may play a pivotal role in regulating immune responses related to multiple scle rosis.To develop more effective therapies for multiple scle rosis,we should further uncover the disease processes involved in multiple sclerosis and gain a better understanding of the gut-brain axis.This review provides an overview of the role of the gut flora in multiple scle rosis.

关键词： gut flora gut-brain axis multiple sclerosis pathogenesis treatment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The effect of glutathione on glucosinolate biosynthesis through the sulfur assimilation pathway in pakchoi associated with the growth conditions

引用

Horticultural Plant Journal 2024年第2期10卷 473-487页

作者： Biao zhu Zhile liang Dan Wang Chaochao He zhujun zhu Jing Yang Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable Ministry of Agriculture and Rural AffairsCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang ProvinceCollege of Horticulture ScienceZhejiang A&F UniversityHangzhouZhejiang 311300China

Glucosinolates(GSLs) are a group of nitrogen-and sulfur-containing secondary metabolites, synthesized primarily in members of the Brassicaceae family, that play an important role in food flavor, plant antimicrobial activity, resistance to insect attack, stress tolerance, and human anti-cancer effects. As a sulfur-containing compound, glutathione has a strong connection with GSLs biosynthesis as a sulfur donor or redox system, and exists in reduced(glutathione;GSH) and oxidized(glutathione disulfide;GSSG) forms. However, the mechanism of GSH regulating GSLs biosynthesis remainds unclear. Hence, the exogenous therapy to pakchoi under normal growth condition and sulfur deficiency condition were conducted in this work to explore the relevant mechanism. The results showed that exogenous application of buthionine sulfoximine, an inhibitor of GSH synthesis, decreased the transcript levels of GSLs synthesis-related genes and transcription factors, as well as sulfur assimilation-related genes under the normal growth condition. Application of exogenous GSH inhibited the expression of GSLs synthesis-and sulfur assimilation-related genes under the normal condition, while the GSLs biosynthesis and the sulfur assimilation pathway were activated by exogenous application of GSH when the content of GSH in vivo of plants decreased owing to sulfur deficiency. Moreover,exogenous application of GSSG increased the transcript levels of GSLs synthesis-and sulfur assimilation-related genes under the normal growth condition and under sulfur deficiency. The present work provides new insights into the molecular mechanisms of GSLs biosynthesis underlying glutathione regulation.

关键词： Pakchoi Glucosinolates Reduced glutathione Oxidized glutathione Sulfur assimilation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Screening of transition metal oxides for electrocatalytic nitrate reduction to ammonia at large currents

引用

Nano Research 2024年第5期17卷 3902-3910页

作者： Qiongfei Wu Weijie zhu Dongxu Ma Chao liang Zhoucheng Wang Hanfeng liang State Key Laboratory of Physical Chemistry of Solid Surfaces Tan Kah Kee Innovation LaboratoryCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China College of Chemical Engineering Tianjin UniversityTianjin 300072China

Electrochemical nitrate reduction reaction(NO_(3)RR)towards ammonia,as an emerging and appealing technology alternative to the energy-intensive Haber-Bosch process and inefficient nitrogen reduction reaction,has recently aroused wide concern and research.However,the current research of the NO_(3)RR towards ammonia lacks the overall performance comparison of various electrocatalysts.Given this,we here make a comparison of 12 common transition metal oxide catalysts for the NO_(3)RR under a high cathodic current density of 0.25 A·cm^(-2),wherein Co_(3)O_(4) catalyst displays the highest ammonia Faradaic efficiency(85.15%)and moderate activity(ca.-0.25 V vs.reversible hydrogen electrode).Other external factors,such as nitrate concentrations in the electrolyte and applied potential ranges,have also been specifically investigated for the NO_(3)RR.

关键词： nitrate reduction reaction ammonia production transition metal oxides Co_(3)O_(4)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Day-ahead P2P Energy Sharing Strategy Among Energy Hubs Considering Flexibility of Energy Storage and Loads

引用

CSEE Journal of Power and Energy Systems 2024年第3期10卷 1105-1118页

作者： Penghua Li Wanxing Sheng Qing Duan Jun liang Cunhao zhu China Electric Power Research Institute Beijing 100192China School of Engineering Cardiff UniversityUK IEEE

Multi-energy systems are one of the key technologies to tackle energy crisis and environmental pollution.An energy hub(EH)is a minimum multi-energy system.Interconnection of multiple EHs through energy routers(ERs)can realize mutual energy assistance.This paper proposes a peer-to-peer(P2P)energy sharing strategy between EHs including ERs in an interconnected system,which is divided into two levels.In the lower level,a method of determining the charging/discharging constraints of energy storage devices is proposed.Based on the Lyapunov optimization method,virtual queues are used to model the energy storage devices and flexible loads in the system.The objective is to minimize the overall operating cost of the interconnected system.In the upper level,a non-cooperative game model is introduced to minimize the cost of purchasing power from other EHs for each EH.A best response-based method is adapted to find the Nash equilibrium.The simulation outcomes demonstrate that application of the proposed strategy can reduce operating costs of an interconnected system and each EH.On basis of a real-world dataset of interconnected EHs,both analytical and numerical results show the effectiveness of the proposed strategy.

关键词： Energy hub energy router Lyapunov optimization non-cooperative game peer to peer virtual queue

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Four centrosome-related genes to predict the prognosis and drug sensitivity of patients with colon cancer

引用

World Journal of Gastrointestinal Oncology 2024年第5期16卷 1908-1924页

作者： Hui-Yan Wang Yan Diao Pei-zhu Tan Huan liang Department of Clinical Laboratory Harbin Medical University Cancer HospitalHarbin 150086Heilongjiang ProvinceChina Department of Clinical Laboratory Heilongjiang Province HospitalHarbin 150000Heilongjiang ProvinceChina Translational Medicine Center of Northern China Harbin Medical UniversityHarbin 150081Heilongjiang ProvinceChina

BACKGROUND As the primary microtubule organizing center in animal cells,centrosome abnormalities are involved in human colon cancer.AIM To explore the role of centrosome-related genes(CRGs)in colon cancer.METHODS CRGs were collected from public databases.Consensus clustering analysis was performed to separate the Cancer Genome Atlas cohort.Univariate Cox and least absolute shrinkage selection operator regression analyses were performed to identify candidate prognostic CRGs and construct a centrosome-related signature(CRS)to score colon cancer patients.A nomogram was developed to evaluate the CRS risk in colon cancer patients.An integrated bioinformatics analysis was conducted to explore the correlation between the CRS and tumor immune microenvironment and response to immunotherapy,chemotherapy,and targeted therapy.Single-cell transcriptome analysis was conducted to examine the immune cell landscape of core prognostic genes.RESULTS A total of 726 CRGs were collected from public databases.A CRS was constructed,which consisted of the following four genes:TSC1,AXIN2,COPS7A,and MTUS1.Colon cancer patients with a high-risk signature had poor survival.Patients with a high-risk signature exhibited decreased levels of plasma cells and activated memory CD4+T cells.Regarding treatment response,patients with a high-risk signature were resistant to immunotherapy,chemotherapy,and targeted therapy.COPS7A expression was relatively high in endothelial cells and fibroblasts.MTUS1 expression was high in endothelial cells,fibroblasts,and malignant cells.CONCLUSION We constructed a centrosome-related prognostic signature that can accurately predict the prognosis of colon cancer patients,contributing to the development of individualized treatment for colon cancer.

关键词： Colon cancer Centrosome Signature Prognostic Immune microenvironment Therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The use of electrokinetics promises a sustainable mining future

引用

The Innovation 2024年第3期5卷 17-18页

作者： Gaofeng Wang Xiaoliang liang Jianxi zhu Hongping He CAS Key Laboratory of Mineralogy and Metallogeny/Guangdong Provincial Key Laboratory of Mineral Physics and Material Guangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou 510640China CAS Center for Excellence in Deep Earth Science Guangzhou 510640China University of the Chinese Academy of Sciences Beijing 100049China

Moving toward a zero-carbon economy is an ambitious goal of all human beings.Utilization of various metal resources plays a central role in such a transition.Rare earth elements(REEs),especially heavy REEs(HREEs),are crucial for the development of future green and zero-carbon economies due to their irreplaceable roles in many applications of renewable energy technologies;e.g.,solar panels,wind turbines,and hybrid vehicle batteries.Ion-adsorption rare earth deposits(IADs)are the primary repositories for HREEs and supply more than 95%of the global HREE demand.1 However,current mining technologies using excessive ammonium salts have caused devastating harm to the local environment while exhibiting a low recovery rate of REEs,a long mining period,and landslide risks.Since 2018,the adoption of ammonium salt-based techniques in REE mining has been prohibited,exacerbating the scarcity of REEs that could lead to disruptions of the supply chain.Moreover,metals are currently almost exclusively extracted from their ores via physical excavation or chemical leaching.These energy-intensive and environmentally damaging techniques remain among the foremost CO_(2) emitters.The mining industry is in desperate need of a new generation of mining technology.

关键词： mining replace earth

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A novel matrix topology-based analysis method of thermal circuits for reliability optimization of HSER

引用

Chinese Journal of Aeronautics 2024年第4期37卷 479-492页

作者： Huimin liang Bo LI Jiaxin YOU Shan JIANG Mengtong zhu Aobo WANG Department of Electrical Engineering Harbin Institute of TechnologyHarbin 150001China Nanjing Research Institute of Electronics Technology Nanjing 210008China

Hermetically Sealed Electromagnetic Relay(HSER), used in aviation and aerospace,demands high reliability due to its critical applications. Given its complex operating conditions, efficient thermal analysis is essential for optimizing reliability. The commonly used Finite Element Method(FEM) is often time-consuming and may not be efficient or adaptable for complex multi-dimensional system calculations and design processes. This paper introduces an analysis method for thermal networks based on matrix perspective technology, encompassing matrix transformation, backpropagation of the heat path model, temperature rise calculation, solution comparison, and product implementation. Using the similarity theory of heat circuits, a basic thermal unit is established. Based on the fundamental connection between key components, a thermal network for a typical HSER is designed. An experimental system is set up, and the thermal network model's accuracy is confirmed using test data. Employing the topology analysis method, the topology of the thermal network is analyzed under both coil-energized and de-energized states. Potential thermal paths are identified, leading to optimized solutions for the HSER. Utilizing these solutions, the thermal path matrix topology model is backpropagated to the thermal path for temperature rise calculations. When compared to prototype HSER test data, the efficiency and accuracy of this matrix topology-based analysis method are confirmed.

关键词： Thermal networks HSER(Hermetically Sealed Electromagnetic Relay) Heat-circuit topology model Heat-circuit matrix Optimization design

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：