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Using bovine pituitary extract to increase proliferation of keratocytes and maintain their phenotype in vitro

International Journal of Ophthalmology(English edition) 2013年第6期6卷 758-765页

作者： Zhong-Zhong Xu Zhi-Jie Li lian-xin du Jing Li Li-Ya Wang Department of Ophthalmology the First Affiliated Hospital of Zhengzhou University Key Laboratory of Corneal Disease Henan Eye Institute Department of Pediatrics Baylor College of Medicine

AIM:To investigate the effects of bovine pituitary extract on the proliferation of keratocytes and maintaining the keratocyte phenotype/n v/***:Single keratocytes were isolated by enzyme digestion for in vitro *** groups were designed according to the different culture media:a bovine pituitary extract(BPE)group,a fetal bovine serum(FBS)group and the control *** phenotypes and proliferation of cultured cells were evaluated by morphology,immunofluorescent staining and mRNA expression of CD34,Lumican,VSX1,α-SMA and proliferating cell nuclear antigen(PCNA).in the BPE group,cells underwent serial subcultivation,and their phenotypes were identified by immunofluorescent *** analyze the proliferation of keratocytes in different concentrations of BPE,six different concentrations were designed to ascertain the most appropriate ***:In the BPE group,the cells spread out and presented dendritic morphology,and their dendrites connected to one another to form *** the third passage,most cells maintained their *** the FBS group,the cells exhibited a dendritic appearance in early cultured stages,but their morphology subsequently changed into a fibroblast-like *** number ofdendritic cells in BPE group was more than FBS and control *** staining and realtime polymerase chain reaction(PCR)confirmed that few keratocytes underwent fibroblastic transformation in the BPE and control groups,and that proliferation was higher in the BPE group than in the control *** the proliferation was higher in the FBS group,many keratocytes underwent fibroblastic *** analysis of cell morphology and mRNA expressions of CD34,PCNA and VSX1 in six group showed that different concentrations of BPE affected the proliferation obviously but didn’t affect the keratocyte phenotype,and the concentration of 40μg/mL was the most appropriate ***:BPE can improve the proliferation of keratocytes and m

关键词： keratocyte bovine pituitary extract proliferation phenotype fibroblastic transformation

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Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury

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Neural Regeneration Research 2025年第11期20卷 3287-3301页

作者： Ziwei Fan Mengxian Jia Jian Zhou Zhoule Zhu Yumin Wu Xiaowu Lin Yiming Qian Jiashu lian xin Hua Jianhong Dong Zheyu Fang Yuqing Liu Sibing Chen Xiumin Xue Juanqing Yue Minyu Zhu Ying Wang Zhihui Huang Honglin Teng Department of Orthopedics(Spine Surgery) the First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina School of Pharmacy Hangzhou Normal UniversityHangzhouZhejiang ProvinceChina Department of Neurology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Pathology Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhouZhejiang ProvinceChina Department of Clinical Research Center Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhouZhejiang ProvinceChina

The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord *** of shifting microglia polarization from M1(neurotoxic and proinflammatory type)to M2(neuroprotective and anti-inflammatory type)after spinal cord injury appears to be *** possesses an anti-inflammatory biological ***,its roles and the underlying molecular mechanisms in spinal cord injury remain *** this study,we found that tryptanthrin inhibited microglia-derived inflammation by promoting polarization to the M2 phenotype in *** promoted M2 polarization through inactivating the cGAS/STING/NF-κB ***,we found that targeting the cGAS/STING/NF-κB pathway with tryptanthrin shifted microglia from the M1 to M2 phenotype after spinal cord injury,inhibited neuronal loss,and promoted tissue repair and functional recovery in a mouse model of spinal cord ***,using a conditional co-culture system,we found that microglia treated with tryptanthrin suppressed endoplasmic reticulum stress-related neuronal *** together,these results suggest that by targeting the cGAS/STING/NF-κB axis,tryptanthrin attenuates microglia-derived neuroinflammation and promotes functional recovery after spinal cord injury through shifting microglia polarization to the M2 phenotype.

关键词： cGAS/STING functional recovery microglia neuroinflammation neuroprotection nuclear factor-κB polarization spinal cord injury tryptanthrin

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Molecular cloning and identification of mouse epididymis-specific gene mHongl, the homologue of rat HongrES1

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Asian Journal of Andrology 2012年第4期14卷 626-634页

作者： Shuang-Gang Hu Han du Guang-xin Yao Yong-lian Zhang Shanghai Key Laboratory for Molecular Andrology State Key Laboratory of Molecular BiologyInstitute of Biochemistry and Cell BiologyShanghai Institutes for Biologica SciencesChinese Academy of SciencesShanghai 200031China Shanghai Institute of Planned Parenthood Research Shanghai 200031China

Previous studies have shown that rat epididymis-specific gene HongrES1 plays important roles in sperm capacitation and fertility. In this study, we cloned the mouse homologue gene by sequence alignment and RT-PCR methods and designated it as mHongl. The mHongl gene is located on chromosome 12p14, spanning five exons. The cDNA sequence consists of 1257 nucleotides and encodes a 419 amino-acid protein with a predicted N-terminal signal peptide of 20 amino acids. The mHongl mRNA shows similarity with HongrES1 in the expression patterns：（i） specific expression in epididymal tissue, especially in the cauda region; and （ii） androgen-dependence but testicular fluid factor independence. Its protein product shows 71% similarity with HongrES 1 and contains a classical serpin domain as does HongrES1. A polyclonal antibody against mHongl with high specificity and sensitivity was raised. Like HongrES1, the mHongl protein shows a checker-board expression pattern in the epididymal epithelium and is secreted into the epididymal lumen. The mHongl protein shows higher glycosylation than HongrES1. Although both of them are deposited onto the sperm head surface, mHongl is localized to the equatorial segment, which is different from that of HongrES 1. The mHongl protein can be removed from the sperm membrane by high ionic strength and therefore can be classed as an extrinsic membrane protein. Collectively, we conclude that mHongl is the homologue of HongrES1 and the present work paves the way for establishing animal models to elucidate the precise functions of HongrES1 and mHongl.

关键词： androgen epididymal secretory protein epididymis glycosylation sperm maturation

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抗棉铃虫组织蛋白酶B单克隆抗体的制备及鉴定

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山东大学学报（理学版） 2004年第6期39卷 116-120页

作者：杨晓梅侯立静康翠洁邵红莲张新昌杜欣军王金星赵小凡山东大学生命科学学院山东济南250100

分别以棉铃虫组织表达的组织蛋白酶B和基因重组的大肠杆菌表达的组织蛋白酶B为抗原免疫BALB c小鼠 ,选择血清抗体滴度高的免疫小鼠 ,取其脾细胞和Sp2 0骨髓瘤细胞进行细胞融合 .经ELISA法筛选阳性克隆和有限稀释法克隆细胞 ,共获得分... 详细信息

分别以棉铃虫组织表达的组织蛋白酶B和基因重组的大肠杆菌表达的组织蛋白酶B为抗原免疫BALB c小鼠 ,选择血清抗体滴度高的免疫小鼠 ,取其脾细胞和Sp2 0骨髓瘤细胞进行细胞融合 .经ELISA法筛选阳性克隆和有限稀释法克隆细胞 ,共获得分泌抗组织蛋白酶B的单克隆抗体杂交瘤细胞 3株 ,分别命名为 9D5 ,10 0E2和10 0H6 .以ELISA法和Westernblotting法对 3株杂交瘤细胞株分泌的单克隆抗体进行了特异性鉴定 ,其中 9D5和10 0E2能够同时识别两种不同来源的组织蛋白酶B ,而 10 0H6只能识别基因重组的大肠杆菌表达的组织蛋白酶B .

关键词：棉铃虫组织蛋白酶B 单克隆抗体 ELISA Westem blotting

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Resistance risk and molecular mechanism associated with resistance to picoxystrobin in Colletotrichum truncatum and Colletotrichum gloeosporioides

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Journal of Integrative Agriculture 2023年第12期22卷 3681-3693页

作者： SHI Niu-niu lian Jin-pan QIU De-zhu CHEN Fu-ru du Yi-xin Institute of Plant Protection Fujian Academy of Agricultural SciencesFuzhou 350013P.R.China Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests Fuzhou 350013P.R.China Crop Research Institute Ningxia Academy of Agriculture and Forestry SciencesYinchuan 750002P.R.China

Anthracnose,caused by Colletotrichum truncatum and ***,is amongst the most serious diseases of soybean in ***,a quinone outside inhibitor fungicide,is commonly used for the control of *** resistance risk and mechanism in *** and *** are *** this study,the sensitivities of 128 *** and 121 *** isolates to picoxystrobin were investigated,and unimodal distributions were observed with average EC_(50)values of 0.7740 and 1.1561μg mL^(-1),*** picoxystrobin-resistant mutants of *** and six mutants of *** were acquired,with EC_(50)values varying from 5.40-152.96 and 13.53-28.30μg mL^(-1),*** to the parental isolates,mutants showed similar or higher relative fitness in conidial production and germination,and ***,the resistance risk of *** and *** to picoxystrobin is moderate to *** was positive cross-resistance between picoxystrobin and pyraclostrobin,but not between picoxystrobin and fluazinam,difenoconazole,or *** G143S mutation in Cyt b protein was detected in seven high-resistant mutants of ***(RF>100),and G137R occurred in four moderate-resistant mutants(RF<_(50)).Contrastingly,there were no point mutations in Cyt b of any *** *** docking confirmed that two mutations conferred different resistance levels to *** greenhouse trials,picoxystrobin did not control mutants with the G143S mutation,those bearing G137R or no point mutation were somewhat controlled,but at a lower level compared to wild-type *** results showed that integrated management strategies should be implemented to preserve fungicide effectiveness.

关键词： Colletotrichum truncatum Colletotrichum gloeosporioides picoxystrobin point mutation Cyt b molecular docking

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Existence of multiple solutions for the laminar flow in a porous channel with suction at both slowly expanding and contracting walls

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International Journal of Minerals,Metallurgy and Materials 2011年第4期18卷 494-501页

作者： xin-hui Si lian-cunZheng xin-xin Zhang Ying Chao Applied Science School University of Science and Technology Beijing Beijing 100083 China Department of Mechanical Engineering University of Science and Technology Beijing Beijing 100083 China Chemical Defense Institute of the PLA Beijing 102205 China

The asymptotic behavior of solutions of a similarity equation for the laminar flow in a porous channel with suction at both expanding and contracting walls has been obtained by using a singular perturbation ***,in the matching process,this solution neglects exponentially small *** take into account these exponentially small terms,a method involving the inclusion of exponentially small terms in a perturbation series was used to find two of the solutions *** series involving the exponentially small terms and expansion ratio predicts dual ***,the result indicates that the expansion ratio has much important influence on the solutions.

关键词： laminar flow porous channels multiple solutions singular perturbation method

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Unsteady heat and mass transfer in MHD flow over an oscillatory stretching surface with Soret and dufour efects

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Acta Mechanica Sinica 2013年第5期29卷 667-675页

作者： lian-Cun Zheng xin Jin xin-xin Zhang Jun-Hong Zhang School of Mathematics and Physics University of Science and TechnologyBeijing School of Mechanical Engineering University of Science and Technology Beijing Naval University of Engineering

In this paper, we study the unsteady coupled heat and mass transfer of two-dimensional MHD fluid over a moving oscillatory stretching surface with Soret and dufour effects. Viscous dissipation effects are adopted in the energy equation. A uniform magnetic field is applied vertically to the flow direction. The governing equations are reduced to non-linear coupled partial differential equations and solved by means of homotopy analysis method （HAM）. The effects of some physical parameters such as magnetic parameter, dufour number, Soret number, the Prandtl num- ber and the ratio of the oscillation frequency of the sheet to its stretching rate on the flow and heat transfer characteristics are illustrated and analyzed.

关键词： Viscous conducted fluid Magnetic field Os- cillatory stretching surface Heat and mass transfer HAM solution

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Developing a new model system for potato genetics by androgenesis

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Journal of Integrative Plant Biology 2021年第4期63卷 628-633页

作者： Miru du Ting Wang Qun lian Xiaojie Zhang Guohui xin Yuanyuan Pu Glenn JBryan Jianjian Qi Inner Mongolia Potato Engineering and Technology Research Center Inner Mongolia UniversityHohhot 010021China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China Cell and Molecular Sciences The James Hutton InstituteInvergowrieDundee DD25DAUK

High heterozygosity and tetrasomic inheritance complicate studies of asexually propagated polyploids,such as *** genetics approaches,especially mutant library construction,can be an ideal choice if a proper mutagenesis genotype is ***,we aimed to generate a model system for potato research using anther cultures of Solanum verrucosum,a self-compatible diploid potato with strong late blight *** of the 23 regenerants obtained(SVA4,SVA7,SVA22,SVA23,SVA32,and SVA33)were diploids,and their homozygosity was estimated to be>99.99%with 22 polymorphic In Del *** lines-SVA4 and SVA32-had reduced stature(plant height≤80 cm),high seed yield(>1,000 seeds/plant),and good tuber set(>30 tubers/plant).We further confirmed the full homozygosity of SVA4 and SVA32 using wholegenome *** two regenerants possess all the characteristics of a model plant:diploidy,100%homozygosity,self-compatibility,and amenability to ***,we have successfully generated two lines,SVA4 and SVA32,which can potentially be used for mutagenesis and as model plants to rejuvenate current methods of conducting potato research.

关键词： diploid potato homozygous model system self-compatible Solanum verrucosum

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Influence of phosphorous on nucleation and growth of grains during solidification in a NiCrFe alloy

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Journal of Materials Science & Technology 2019年第1期35卷 134-141页

作者： xintong lian Wenru Sun Litao Chang Fang Liu xin xin Institute of Metal Research Chinese Academy of Sciences School of Materials Science and Engineering University of Science and Technology of China

The influence of phosphorus on the solidification of a NiCrFe model alloy has been investigated through a series of analytical techniques. It was found that increasing phosphorous additions greatly influenced the as-cast microstructure, changing from equiaxed grains to columnar grains in the center of ingots. It is indicated that the increase of phosphorous reduced the nucleation of grains and promoted dendrite growth during solidification. Furthermore, the addition of phosphorus in alloys also caused the segregation and phase transformation at grain boundaries and in interdendritic regions. The mechanism by which phosphorus influence the solidification of alloys was discussed based on experimental results as well.

关键词： NiCrFe alloy Phosphorus Segregation Nucleation Grain growth

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Selective sensing of Cr^(VI) and Fe^(Ⅲ) ions in aqueous solution by an exceptionally stable Tb^(Ⅲ)-organic framework with an AIE-active ligand

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Chinese Chemical Letters 2021年第8期32卷 2443-2447页

作者： Jing-Jing Pang Rui-Huan du xin lian Zhao-Quan Yao Jian Xu Xian-He Bu School of Materials Science and Engineering National Institute for Advanced MaterialsTianjin Key Laboratory of Metal and Molecule-Based Material ChemistryNankai UniversityTianjin 300350China State Key Laboratory of Elemento-Organic Chemistry College of ChemistryNankai UniversityTianjin 300071China

We herein report a new lanthanide metal-organic framework(MOF) that exhibits excellent chemical stability,especially in the aqueous solution over a wide pH range from 1 to *** contrast to many reported lanthanide MOFs,this Tb-based MOF emits cyan fluorescence inherited from the integrated AIEactive ligand,rather than Ln3+ *** remarkably,its fluorescence signal features a highly selective and sensitive "turn-off" response toward CrO_(4)^(2-),Cr_(2)O_(7)^(2- )and Fe^(3+) ions,highlighted with the low detection limits down to 68.18,69.85 and 138.8 ppm,***,the exceptional structural stability and sensing performance render this material able to be a superior luminescent sensor for heavy metal ions in wastewater.

关键词： Lanthanide metal-organic framework Aggregation-induced emission Heavy metal ions Selective luminescence sensing Aqueous-phase sensing

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