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Establishing Modular Cell-Free Expression System for the Biosynthesis of Bicyclomycin from a Chemically Synthesized Cyclodipeptide

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Chinese Journal of Chemistry 2024年第4期42卷 384-390页

作者： Yi-Pei Liu Yu-Heng Zhao Wen-Qi Zhang lian wu Linjuan Huang Gong-Li Tang Jun-Bin He Shaanxi Natural Carbohydrate Resource Engineering Research Center College of Food Science and TechnologyNorthwest UniversityXi'anShaanxi710069 China State Key Laboratory of Chemical Biology Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of Sciences(CAS)CASShanghai200032 China School of Chemistry and Materials Science Hangzhou Institute for Advanced StudyUniversity of CAS1 Sub-lane XiangshanHangzhouZhejiang310024 China

Cell-free expression systems have emerged as a versatile and powerful platform for metabolic engineering,biosynthesis and synthetic biology ***,successful examples of the synthesis of complex natural products using this system are still ***,a structurally unique and complex diketopiperazine alkaloid,is a clinically promising antibiotic that selectively inhibits the transcription termination factor ***,we established a modular cell-free expression system with cascade catalysis for the biosynthesis of bicyclomycin from a chemically synthesized *** six cell-free expressed biosynthetic enzymes,including five iron-andα-ketoglutarate-dependent dioxygenases and one cytochrome P450 monooxygenase,were active in converting their substrates to the corresponding *** co-expressed enzymes in the cell-free module were able to complete the related partial *** vitro biosynthesis of bicyclomycin was also achieved by reconstituting the entire biosynthetic pathways(i.e.,six enzymes)using the modular cell-free expression *** study demonstrates that the modular cell-free expression system can be used as a robust and promising platformforthe biosynthesis of complex antibiotics.

关键词： Natural products Bicyclomycin Biosynthesis Iron-andα-ketoglutarate-dependent dioxygenases Cytochrome P450 monooxygenase Cell-freeprotein synthesis Enzyme catalysis

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Epicardial adipose tissue in obesity with heart failure with preserved ejection fraction: Cardiovascular magnetic resonance biomarker study

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World Journal of Cardiology 2024年第3期16卷 149-160页

作者： Ju-Wei Shao Bing-Hua Chen Kamil Abu-Shaban Ahmad Baiyasi lian-Ming wu Jing Ma Department of Radiology The Affiliated Hospital of Yunnan UniversityKunming 650021Yunnan ProvinceChina Department of Radiology Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Radiology University of Toledo College of MedicineToledoOH 43623United States Department of Radiology Wayne State University School of MedicineDetroitMI 48201United States Department of Endocrinology Ren Ji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China

BACKGROUND Obesity has become a serious public health issue,significantly elevating the risk of various *** is a well-established contributor to Heart failure with preserved ejection fraction(HFpEF).Evaluating HFpEF in obesity is *** adipose tissue(EAT)has emerged as a valuable tool for validating prognostic biomarkers and guiding treatment ***,assessing EAT is of paramount *** magnetic resonance(CMR)imaging is acknowledged as the gold standard for analyzing cardiac function and *** hope to use CMR to assess EAT as a bioimaging marker to evaluate HFpEF in obese *** To assess the diagnostic utility of CMR for evaluating heart failure with preserved ejection fraction[HFpEF;left ventricular(LV)ejection fraction≥50%]by measuring the epicardial adipose tissue(EAT)volumes and EAT mass in obese *** Sixty-two obese patients were divided into two groups for a case-control study based on whether or not they had heart failure with *** two groups were defined as HFpEF+and HFpEF-.LV geometry,global systolic function,EAT volumes and EAT mass of all subjects were obtained using cine magnetic resonance *** Forty-five patients of HFpEF-group and seventeen patients of HFpEF+group were *** mass index(g/m2)of HFpEF+group was higher than HFpEF-group(P<0.05).In HFpEF+group,EAT volumes,EAT volume index,EAT mass,EAT mass index and the ratio of EAT/[left atrial(LA)left-right(LR)diameter]were higher compared to HFpEF-group(P<0.05).In multivariate analysis,Higher EAT/LA LR diameter ratio was associated with higher odds ratio of *** EAT/LA LR diameter ratio is highly associated with HFpEF in obese *** is plausible that there may be utility in CMR for assessing obese patients for HFpEF using EAT/LA LR diameter ratio as a diagnostic *** prospective studies,are needed to validate these proof-of-concept findings.

关键词： Heart failure with preserved ejection fraction Epicardial adipose tissue Obesity Cardiac magnetic resonance

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Chain Elongation Using Native Soil Inocula:Exceptional n-Caproate Biosynthesis Performance and Microbial Mechanisms

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Engineering 2024年第8期39卷 262-272页

作者： Lin Deng Yang Lv Tian Lan Qing-lian wu Wei-Tong Ren Hua-Zhe Wang Bing-Jie Ni Wan-Qian Guo State Key Laboratory of Urban Water Resource and the Environment Harbin Institute of TechnologyHarbin 150090China Centre for Technology in Water and Wastewater&School of Civil and Environmental Engineering University of Technology SydneySydneyNSW 2007Australia

This study demonstrates the feasibility and effectiveness of utilizing native soils as a resource for inocula to produce n-caproate through the chain elongation(CE)platform,offering new insights into anaerobic soil *** results reveal that all five of the tested soil types exhibit CE activity when supplied with high concentrations of ethanol and acetate,highlighting the suitability of soil as an ideal source for n-caproate *** with anaerobic sludge and pit mud,the native soil CE system exhibited higher selectivity(60.53%),specificity(82.32%),carbon distribution(60.00%),electron transfer efficiency(165.00%),and conductivity(0.59 ms∙cm^(-1)).Kinetic analysis further confirmed the superiority of soil in terms of a shorter lag time and higher yield.A microbial community analysis indicated a positive correlation between the relative abundances of Pseudomonas,Azotobacter,and Clostridium and n-caproate ***,metagenomics analysis revealed a higher abundance of functional genes in key microbial species,providing direct insights into the pathways involved in n-caproate formation,including in situ CO_(2)utilization,ethanol oxidation,fatty acid biosynthesis(FAB),and reverse beta-oxidation(RBO).The numerous functions in FAB and RBO are primarily associated with Pseudomonas,Clostridium,Rhodococcus,Stenotrophomonas,and Geobacter,suggesting that these genera may play roles that are involved or associated with the CE ***,this innovative inoculation strategy offers an efficient microbial source for n-caproate production,underscoring the importance of considering CE activity in anaerobic soil microbial ecology and holding potential for significant economic and environmental benefits through soil consortia exploration.

关键词： Soil Chain elongation n-caproate Reverse beta-oxidation Fatty acid biosynthesis Metagenomics

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Endoscopic polidocanol foam sclerobanding for the treatment of grade Ⅱ-Ⅲ internal hemorrhoids:A prospective,multi-center,randomized study

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World Journal of Gastroenterology 2024年第27期30卷 3326-3335页

作者： Chun-Ying Qu Fei-Yu Zhang Yi Zhang Ming-Ming Li Zheng-Hong Li Mei-Hong Cai Lei-Ming Xu Feng Shen Wen Wang wu-lian Lin Feng-Yu Gao Hao Zhang Guang-Yu Chen Department of Gastroenterology&Endoscopy Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Gastroenterology The 900th Hospital of the People’s Liberation Army Joint Service Support ForceFuzhou 350025Fujian ProvinceChina Department of Endoscopy Shandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao UniversityJinan 250014Shandong ProvinceChina Department of Endoscopy Baoshan People’s Hospital of Yunnan ProvinceBaoshan 678000Yunnan ProvinceChina Department of Gastroenterology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China

BACKGROUND Endoscopic rubber band ligation(ERBL)is a nonsurgical technique for the treatment of symptomatic internal hemorrhoids but is limited by recurrence and post-procedural *** To evaluate satisfaction,long-term recurrence,and post-procedural pain in managing internal hemorrhoids using a combination of polidocanol foam sclerotherapy and *** This was a prospective,multicenter,randomized study.A total of 195 consecutive patients diagnosed with grade II-III internal hemorrhoids were enrolled from four tertiary hospitals and randomly divided into a cap-assisted endoscopic polidocanol foam sclerobanding(EFSB)or an ERBL *** patients were followed-up for 12 ***-based severity and post-procedural pain were assessed using a hemorrhoid severity score(HSS)and a visual analog scale(VAS).Continuous variables were reported as medians and interquartile *** One hundred and ninety-five patients were enrolled,with 98 in the EFSB *** was lower in the EFSB group than in the ERBL group at 8 weeks[4.0(3.0-5.0)vs 5.0(4.0-6.0),P=0.003]and 12-month[2.0(1.0-3.0)vs 3.0(2.0-3.0),P<0.001]of *** prolapse recurrence rate was lower in the EFSB group at 12 months(11.2%vs 21.6%,P=0.038).Multiple linear regression analysis demonstrated that EFSB treatment[B=-0.915,95%confidence interval(CI):−1.301 to−0.530,P=0.001]and rubber band number(B=0.843,95%CI:0.595-1.092,P<0.001)were negatively and independently associated with the VAS score 24 hours *** median VAS was lower in the EFSB group than in the ERBL[2.0(1.0-3.0)vs 3.0(2.0-4.0),P<0.001].CONCLUSION Cap-assisted EFSB provided long-term satisfaction and effective relief from the recurrence of prolapse and pain 24 hours post-procedure.

关键词： Internal hemorrhoids Endoscopic therapy Polidocanol foam Sclerotherapy Rubber band ligation Sclerobanding

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揭示氨对正己酸生产影响的机制——影响途径、关键酶和微生物

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Engineering 2024年第4期35卷 180-190页

作者： Qing-lian wu Ke-Xin Yuan Wei-Tong Ren Lin Deng Hua-Zhe Wang Xiao-Chi Feng He-Shan Zheng Nan-Qi Ren Wan-Qian Guo State Key Laboratory of Urban Water Resource and Environment Harbin Institute of TechnologyHarbin 150090China College of Architecture and Environment Sichuan UniversityChengdu 610065China State Key Laboratory of Urban Water Resource and Environment School of Civil and Environmental EngineeringHarbin Institute of Technology(Shenzhen)Shenzhen 518055China College of Chemistry and Chemical Engineering Qiqihar UniversityQiqihar 161006China

n-Caproate,which is produced via chain elongation(CE)using waste biomass,can supply various fossilderived products,thus advancing the realization of carbon *** released from the degradation of nitrogen-rich waste biomass can act as a nutrient or an inhibitor in anaerobic bioprocesses,including CE,with the distinction being primarily dependent on its ***,the optimal concentration of ammonia and the threshold of toxicity for open-culture n-caproate production using ethanol as an electron donor,along with the underlying mechanisms,remain *** study revealed that the optimal concentration of ammonia for n-caproate production was 2.0 g∙L^(-1),whereas concentrations exceeding this threshold markedly suppressed the CE *** of the mechanism revealed the involvement of two forms of ammonia(i.e.,ammonium ions and free ammonia)in this inhibitory *** ammonia levels(5.0 g∙L^(-1))induced excessive ethanol oxidation and suppressed the reverse β-oxidation(RBO)process,directly leading to the enhanced activities of enzymes(phosphotransacetylase and acetate kinase)responsible for acetate formation and diminished activities of butyryl-coenzyme A(CoA):acetyl-CoA transferase,caproyl-CoA:butyryl-CoA transferase,and caproyl-CoA:acetyl-CoA transferase that are involved in the syntheses of n-butyrate and ***,the composition of the microbial community shifted from Paraclostridium dominance(at 0.1 g∙L^(-1)ammonia)to a co-dominance of Fermentimonas,Clostridium sensu stricto 12,and Clostridium sensu stricto 15 at 2.0 g∙L^(-1)***,these CE-functional bacteria were mostly absent in the presence of excessive ammonia(5.0 g∙L^(-1)ammonia).Metagenomic analysis revealed the upregulation of functions such as RBO,fatty acid synthesis,K^(+)efflux,adenosine triphosphatase(ATPase)metabolism,and metal cation export in the presence of 2.0 g∙L^(-1)ammonia,collectively contributing to enhanced n-caproate produc

关键词： Ammonia inhibition Medium chain fatty acids n-Caproate Enzyme Metagenomics

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地铁联络线供电回流模式转换方案设计

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城市轨道交通研究 2024年第9期27卷 176-180页

作者：王键波陈青云吴炼绍兴市轨道交通集团有限公司绍兴312099

[目的]用联络线连接的地铁线路间有可能采用不同的供电回流模式。为提高列车过轨效率,须在联络线内设置回流模式转换段,并对回流模式转换方案进行设计,设计方案应满足使用走行轨与回流轨回流的需求。[方法]以绍兴地铁1号线(以下简称“1... 详细信息

[目的]用联络线连接的地铁线路间有可能采用不同的供电回流模式。为提高列车过轨效率,须在联络线内设置回流模式转换段,并对回流模式转换方案进行设计,设计方案应满足使用走行轨与回流轨回流的需求。[方法]以绍兴地铁1号线(以下简称“1号线”)、绍兴地铁2号线(以下简称“2号线”)一期工程为例,介绍了这2条线路的概况及联络线的具体情况。从运营条件、经济性、工程可实施性等方面,针对联络线内供电回流模式转换提出了3个设计方案(方案一、方案二及方案三),分别对这3个方案进行了优缺点分析。[结果及结论]推荐采用方案三。方案三不仅可以有效解决列车动力损失的问题,减小2条线路之间产生电气导通、迷流的可能性,还可以优化转线过轨流程,提高列车转线过轨效率。

关键词：城市轨道交通供电回流模式联络线专用回流轨接触网

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上海奉浦东桥主桥设计及施工关键技术

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中国市政工程 2024年第2期 6-9,140页

作者：吴连盛上海公路投资建设发展有限公司上海200335

为缓解现行交通压力,利用上海奉浦大桥东侧先期已实施的主桥水中墩基础进行奉浦东桥的续建。奉浦东桥主桥跨径组合(85.15+125.00+125.00+125.00+85.15)m,上部结构采用预应力波形钢腹板连续梁桥,下部结构采用薄壁空心直立式桥墩。主梁为... 详细信息

为缓解现行交通压力,利用上海奉浦大桥东侧先期已实施的主桥水中墩基础进行奉浦东桥的续建。奉浦东桥主桥跨径组合(85.15+125.00+125.00+125.00+85.15)m,上部结构采用预应力波形钢腹板连续梁桥,下部结构采用薄壁空心直立式桥墩。主梁为单箱双室截面,箱内采用钢横隔板与钢横撑混合布置形式。墩顶0号节段采用钢混组合构造,钢箱与混凝土板之间设置焊钉连接件,外围焊钉包裹胶套以避免受力集中。全桥腹板均采用1600型波形钢板,为提高中支点区段腹板稳定性,沿纵向设置T形加劲肋,无需现场浇筑内衬混凝土。波形钢腹板与混凝土顶板间采用双开孔板连接件,并在各节段前端增设梅花形开孔构造并混合配置焊钉以改善连接件受力;腹板与混凝土底板采用焊接角钢的翼缘形结合部。主桥上部结构采用工厂化节段预制、现场悬臂拼装的施工工艺,提高了施工效率及安全性。

关键词：组合连续梁桥波形钢腹板 T形加劲肋节段预制悬臂拼装

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高分辨质谱非靶向筛查分析方法在食品化学危害物监测中的研究进展

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食品科学 2024年第19期45卷 317-331页

作者：石培育宋娟蒋锡兰肖全伟李绍波姚静吴恋刘川林浩陈燕秋陈世奇戴琴吴文林成都市食品检验研究院四川成都611130 国家市场监督管理总局重点实验室(营养与健康化学计量及应用) 北京100029 国家市场监督管理总局重点实验室(调味品监管技术) 重庆401120 中国科学院成都生物研究所四川成都610041 中国科学院大学北京100049

监测食品化学危害物是保障食品质量与安全的关键。基于强分离能力、高质量分辨率和高质量精度的高效液相色谱/高效凝胶色谱-高分辨率质谱联用平台,非靶向筛查可实现多种潜在及未知食品化学危害物的广泛识别。由于食品化学危害物种类多... 详细信息

监测食品化学危害物是保障食品质量与安全的关键。基于强分离能力、高质量分辨率和高质量精度的高效液相色谱/高效凝胶色谱-高分辨率质谱联用平台,非靶向筛查可实现多种潜在及未知食品化学危害物的广泛识别。由于食品化学危害物种类多、采集的数据信息量大及数据分析过程繁琐,非靶向筛查分析方法的开发和应用具有较大的挑战性。选择合适的数据采集模式和高效的数据分析策略是对潜在及未知食品化学危害物进行非靶向筛查的有效途径。本文综述非靶向筛查分析方法所涉及的数据采集模式、分析流程和分析策略,以及近5年来非靶向筛查技术在食品化学危害物筛查中的应用,以促进食品化学危害物非靶向筛查分析方法的开发和应用,为食品中风险因子的提前预警和食品安全监测提供支持。

关键词：食品化学危害物高分辨质谱非靶向筛查数据采集模式分析策略食品质量与安全

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Impacts of land use change on landscape patterns in mountain human settlement:The case study of Hantai District(Shaanxi,China)

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Journal of Mountain Science 2021年第3期18卷 749-763页

作者： WANG Lei wu lian ZHANG Wei School of Architecture Chang'an UniversityXi’an 710064China

Mountain area is an important geographical unit of land,and its ecology is sensitive and *** the past few decades,human activities have caused dramatic changes in land use in mountainous areas,which caused changes in landscape patterns and impacts on the ecological *** is unknown how the mechanism of land use affects the landscape pattern at different *** Hantai District,a typical human settlement in the mountain area in Shaanxi,China,was chosen as the study *** on the remote sensing images,the mathematical models and landscape indexes were adopted to evaluate the impact of land use change from 1998 to 2017 on the landscape pattern at different scales,and its main driving forces were *** results showed that the urbanized land expanded largest from 15.39%to 24.30%,and cultivated land experienced the largest decline from 43.54%to 35.35%.Changes in land use have made the patch morphology of most land types developed from a natural random to a sawtooth shape,and its spatial pattern evolved from a ruleset to a fragmented *** reflects the continuous strengthening of human intervention in the process of regional *** the jurisdiction of Hantai District,the biggest change in landscape pattern is in Hanzhong City and Qili *** improved economy and increasing population and urbanization rate were the main factors that cause these *** research could provide necessary information for understanding the evolution mechanism of land resources in mountainous human settlements for mountainous areas with significant geomorphic differentiation.

关键词： Land use change Land cover change Landscape pattern evolution Transition trend Driving force Mountain regions Hantai District

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烟尘超低排放技术在湿法脱硫塔上的应用研究

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山东工业技术 2024年第2期 97-100页

作者：曹茂雪武连玮山东华嘉特种设备有限公司山东淄博255000

在不对锅炉原有除尘系统进行改动且停炉时间最短的条件下,仅在湿法脱硫塔的上部,安装专利产品—全旋流微湿电除尘除雾装置,使烟尘满足超低排放标准。本文结合应用实践对“全旋流微湿电除尘除雾技术”进行分析研究,为面临烟尘排放难题的... 详细信息

在不对锅炉原有除尘系统进行改动且停炉时间最短的条件下,仅在湿法脱硫塔的上部,安装专利产品—全旋流微湿电除尘除雾装置,使烟尘满足超低排放标准。本文结合应用实践对“全旋流微湿电除尘除雾技术”进行分析研究,为面临烟尘排放难题的企业提供一种新的烟尘解决方案。

关键词：超低排放全旋流微湿电载体旋球流通间隙

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